##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l06n02 kx12-2.3420000000cd0a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17067317 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44449394125626 33.0 33.0 33.0 33.0 33.0 2 32.387656126619085 33.0 33.0 33.0 33.0 33.0 3 32.37030758847451 33.0 33.0 33.0 33.0 33.0 4 36.23391591074332 37.0 37.0 37.0 37.0 37.0 5 36.22499136800471 37.0 37.0 37.0 37.0 37.0 6 36.273994266351295 37.0 37.0 37.0 37.0 37.0 7 36.27243127903466 37.0 37.0 37.0 37.0 37.0 8 36.29888306404575 37.0 37.0 37.0 37.0 37.0 9 36.250255268593186 37.0 37.0 37.0 37.0 37.0 10-11 36.23816329772278 37.0 37.0 37.0 37.0 37.0 12-13 36.22497677871689 37.0 37.0 37.0 37.0 37.0 14-15 38.548753737919085 40.0 40.0 40.0 37.0 40.0 16-17 38.52569369866394 40.0 40.0 40.0 37.0 40.0 18-19 38.50653453615469 40.0 40.0 40.0 37.0 40.0 20-21 38.47373649882989 40.0 40.0 40.0 37.0 40.0 22-23 38.51491561327419 40.0 40.0 40.0 37.0 40.0 24-25 38.44115038702334 40.0 40.0 40.0 37.0 40.0 26-27 38.403502231780195 40.0 40.0 40.0 37.0 40.0 28-29 38.33221624699418 40.0 40.0 40.0 37.0 40.0 30-31 38.2828586356016 40.0 37.0 40.0 37.0 40.0 32-33 38.207642595494065 40.0 37.0 40.0 37.0 40.0 34-35 38.14367557009693 40.0 37.0 40.0 37.0 40.0 36-37 38.07134917573747 40.0 37.0 40.0 37.0 40.0 38-39 37.98267659761637 40.0 37.0 40.0 37.0 40.0 40-41 37.89169949793515 40.0 37.0 40.0 35.0 40.0 42-43 37.78261020756807 40.0 37.0 40.0 33.0 40.0 44-45 37.70712593549413 40.0 37.0 40.0 33.0 40.0 46-47 37.539038942090315 40.0 37.0 40.0 33.0 40.0 48-49 37.416005544398104 40.0 37.0 40.0 33.0 40.0 50-51 35.98810387127631 38.5 35.0 38.5 30.0 40.0 52-53 36.34165706888787 37.0 37.0 40.0 33.0 40.0 54-55 37.02083578807378 37.0 37.0 40.0 33.0 40.0 56-57 37.00921752962109 37.0 37.0 40.0 33.0 40.0 58-59 36.859042109547744 37.0 37.0 40.0 33.0 40.0 60-61 36.748833926269725 37.0 37.0 40.0 33.0 40.0 62-63 36.587625869959524 37.0 37.0 40.0 33.0 40.0 64-65 36.38813619621643 37.0 37.0 40.0 33.0 40.0 66-67 36.13324109466063 37.0 37.0 40.0 33.0 40.0 68-69 35.88426133410424 37.0 37.0 37.0 33.0 40.0 70-71 35.66126119881643 37.0 37.0 37.0 33.0 40.0 72-73 35.43793412286185 37.0 37.0 37.0 33.0 40.0 74-75 35.24138489371235 37.0 37.0 37.0 33.0 38.5 76-77 35.04764410246789 37.0 37.0 37.0 33.0 37.0 78-79 34.89441474603184 37.0 37.0 37.0 33.0 37.0 80-81 34.77084072440911 37.0 33.0 37.0 33.0 37.0 82-83 34.67337294432394 37.0 33.0 37.0 33.0 37.0 84-85 34.572261855803106 37.0 33.0 37.0 33.0 37.0 86-87 34.48556571604078 37.0 33.0 37.0 30.0 37.0 88-89 34.406876634446995 37.0 33.0 37.0 27.0 37.0 90-91 34.31220211706386 37.0 33.0 37.0 27.0 37.0 92-93 34.25433347256631 37.0 33.0 37.0 27.0 37.0 94-95 34.19358279921795 37.0 33.0 37.0 27.0 37.0 96-97 34.13003022091873 37.0 33.0 37.0 27.0 37.0 98-99 34.05558673926312 37.0 33.0 37.0 27.0 37.0 100-101 32.35527883498033 35.0 30.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.051116782046264575 2306 2 0.15873381736587788 2306 3 0.1677016011276038 2306 4 0.2413149390982241 2306 5 0.23283825079830223 2306 6 0.29373875603515387 2306 7 0.3009410441124345 2306 8 0.2360480690738811 2306 9 0.27926369117543715 2306 10-11 0.2893848585652563 2306 12-13 0.3044041979908485 2306 14-15 0.5296103259241818 2306 16-17 0.4797332459692001 2306 18-19 0.5496788459297974 2306 20-21 0.5847008088484316 2306 22-23 0.5309611089514732 2306 24-25 0.53716346470204 2306 26-27 0.541796964652292 2306 28-29 0.6051863229159977 2306 30-31 0.6007385862080028 2306 32-33 0.562491072572449 2306 34-35 0.5764723887173488 2306 36-37 0.5965356235205306 2306 38-39 0.6352742682332391 2306 40-41 0.6201666899797686 2306 42-43 0.6346706162577007 2306 44-45 0.6501635439249043 2306 46-47 0.6202084630323554 2306 48-49 0.6208248548688999 2306 50-51 0.5605221869362396 2306 52-53 0.5770742250472551 2306 54-55 0.6397735950575196 2306 56-57 0.553264239127536 2306 58-59 0.5916710781206973 2306 60-61 0.6010788993902949 2306 62-63 0.6352592654340796 2306 64-65 0.670781594401312 2306 66-67 0.6946091209560095 2306 68-69 0.7062670680582954 2306 70-71 0.6828315359701307 2306 72-73 0.6615257299312844 2306 74-75 0.696069640671503 2306 76-77 0.5910987264608423 2306 78-79 0.6167621048554537 2306 80-81 0.6636665736731828 2306 82-83 0.6655966268688402 2306 84-85 0.6940140879632963 2306 86-87 0.6825698980201054 2306 88-89 0.7039287649070687 2306 90-91 0.7656587071047625 2306 92-93 0.7112367605450842 2306 94-95 0.7194840457799572 2306 96-97 0.6993311545600065 2306 98-99 0.7405855169654814 2306 100-101 0.6641337539080396 2307 1 -0.10735412473265171 2307 2 0.06488472063694672 2307 3 0.16372227551153884 2307 4 0.20519341406095037 2307 5 0.2532212640404268 2307 6 0.26871376261236435 2307 7 0.2568709529083648 2307 8 0.25002487342361235 2307 9 0.24584605985035068 2307 10-11 0.2824785071665019 2307 12-13 0.2710671923782826 2307 14-15 0.516046735202444 2307 16-17 0.5029041220544386 2307 18-19 0.5393856116931985 2307 20-21 0.5276808908444011 2307 22-23 0.5672203170546055 2307 24-25 0.5644535418747125 2307 26-27 0.5559647823318485 2307 28-29 0.5966191695353018 2307 30-31 0.6021681990292009 2307 32-33 0.6045188832363095 2307 34-35 0.6255058667494495 2307 36-37 0.6295941936336575 2307 38-39 0.6455357125899965 2307 40-41 0.652186882034492 2307 42-43 0.6723922136443505 2307 44-45 0.6824490718561549 2307 46-47 0.6417890777828603 2307 48-49 0.67865322240376 2307 50-51 0.6332930586366672 2307 52-53 0.6888120352726403 2307 54-55 0.6662753380199007 2307 56-57 0.6898776851505133 2307 58-59 0.6837082613181025 2307 60-61 0.6776194479301552 2307 62-63 0.6508946793602348 2307 64-65 0.64911339305079 2307 66-67 0.7276424784058051 2307 68-69 0.7210275477566199 2307 70-71 0.6753949512994311 2307 72-73 0.7401233577485229 2307 74-75 0.794877849037718 2307 76-77 0.7655700826828848 2307 78-79 0.8021386505013339 2307 80-81 0.7710863316316079 2307 82-83 0.7707237315781299 2307 84-85 0.7726288713536391 2307 86-87 0.7623617839273251 2307 88-89 0.8089914677045797 2307 90-91 0.8119528840757368 2307 92-93 0.8063107542309567 2307 94-95 0.8377894807532087 2307 96-97 0.878674912781527 2307 98-99 0.8110514579459291 2307 100-101 0.7557009401748118 2201 1 0.0744262952454875 2201 2 0.16737962417344932 2201 3 0.15501910388628204 2201 4 0.21620565295952332 2201 5 0.22282397993843972 2201 6 0.25298404505985417 2201 7 0.2713682680323046 2201 8 0.21739807119594445 2201 9 0.23978195150987602 2201 10-11 0.2365313497374828 2201 12-13 0.24321152016213432 2201 14-15 0.4372197712174568 2201 16-17 0.4554470542197748 2201 18-19 0.43025628523886184 2201 20-21 0.48341908403730116 2201 22-23 0.5118573879176012 2201 24-25 0.519992012787938 2201 26-27 0.46556868925122075 2201 28-29 0.49718243954445995 2201 30-31 0.5058630981339931 2201 32-33 0.5060056564362938 2201 34-35 0.5283842330005228 2201 36-37 0.5336468884810657 2201 38-39 0.5455063155976418 2201 40-41 0.575062284581243 2201 42-43 0.5286990041279935 2201 44-45 0.4949152893200335 2201 46-47 0.5217473551043383 2201 48-49 0.5686508034301383 2201 50-51 0.5096569165856764 2201 52-53 0.5534288957033979 2201 54-55 0.5454987828599158 2201 56-57 0.5841869112266878 2201 58-59 0.5928107574772881 2201 60-61 0.6046694265102275 2201 62-63 0.6182581089108652 2201 64-65 0.6310497168017903 2201 66-67 0.625138416007367 2201 68-69 0.6659935344024177 2201 70-71 0.6526923022463436 2201 72-73 0.6298795676785076 2201 74-75 0.6131091269205413 2201 76-77 0.6915180987552105 2201 78-79 0.67906077432324 2201 80-81 0.660261461628366 2201 82-83 0.6412264810832582 2201 84-85 0.7048094098799425 2201 86-87 0.6769204412677112 2201 88-89 0.6956568387123454 2201 90-91 0.7317188622274173 2201 92-93 0.6984538632952777 2201 94-95 0.6935300467561021 2201 96-97 0.7374502658408844 2201 98-99 0.7147315200636868 2201 100-101 0.6612618096757998 1101 1 -0.0557279612542132 1101 2 0.09744631192313591 1101 3 0.11541837071158056 1101 4 0.1710157367073748 1101 5 0.2114725183041415 1101 6 0.27725021548177153 1101 7 0.26696027183677273 1101 8 0.2876487301196775 1101 9 0.28446472628607467 1101 10-11 0.2509624374355468 1101 12-13 0.2847426623458347 1101 14-15 0.46395291686746276 1101 16-17 0.483157720497168 1101 18-19 0.5150484081354065 1101 20-21 0.492486280684723 1101 22-23 0.5154559729818686 1101 24-25 0.5048719217340576 1101 26-27 0.5307542589166232 1101 28-29 0.5340969332948049 1101 30-31 0.5528339183142563 1101 32-33 0.5514102676403354 1101 34-35 0.5731599852023308 1101 36-37 0.546518182005272 1101 38-39 0.6019580053190978 1101 40-41 0.6236215943854191 1101 42-43 0.5975460417811931 1101 44-45 0.6073522932816786 1101 46-47 0.606354951968747 1101 48-49 0.6108917263805935 1101 50-51 0.5029263451913977 1101 52-53 0.5407219014910609 1101 54-55 0.6171920281414742 1101 56-57 0.6272920911245379 1101 58-59 0.5948169831992729 1101 60-61 0.6251114519374994 1101 62-63 0.6280007500162696 1101 64-65 0.6398880940252738 1101 66-67 0.6728212048336175 1101 68-69 0.6454130028245544 1101 70-71 0.6327564508654362 1101 72-73 0.6454377257354267 1101 74-75 0.6685224372514753 1101 76-77 0.6852209358183998 1101 78-79 0.7219403794071653 1101 80-81 0.6971201421329809 1101 82-83 0.7106287637884421 1101 84-85 0.7319815453119887 1101 86-87 0.7191860708816051 1101 88-89 0.6761044764219335 1101 90-91 0.6952983975611673 1101 92-93 0.738594704969735 1101 94-95 0.7293130199714639 1101 96-97 0.7007713784051575 1101 98-99 0.6598059430827306 1101 100-101 0.6744661817669595 2304 1 -0.05796902948519289 2304 2 0.09700354884213169 2304 3 0.1418220760632991 2304 4 0.16899037356713364 2304 5 0.18096234892239949 2304 6 0.2442045945444704 2304 7 0.23030102591806667 2304 8 0.23610375010782292 2304 9 0.18469180774486205 2304 10-11 0.25666656538500376 2304 12-13 0.2679290034993471 2304 14-15 0.4317244406741665 2304 16-17 0.476124173881864 2304 18-19 0.4836940758689465 2304 20-21 0.5025946149215912 2304 22-23 0.5216643368829637 2304 24-25 0.5327325091841288 2304 26-27 0.4784611609632279 2304 28-29 0.5156253404202289 2304 30-31 0.4904475939876676 2304 32-33 0.49087291114450693 2304 34-35 0.5162909940250842 2304 36-37 0.5449881221088972 2304 38-39 0.6019515221387621 2304 40-41 0.5480675934241503 2304 42-43 0.5879058019657109 2304 44-45 0.6215252589007534 2304 46-47 0.6262016244603004 2304 48-49 0.6617674720343416 2304 50-51 0.5849979225532849 2304 52-53 0.5731953744292682 2304 54-55 0.6252967222926031 2304 56-57 0.6284922568827298 2304 58-59 0.6660574103874595 2304 60-61 0.6477880326483358 2304 62-63 0.6459745314482603 2304 64-65 0.6301003251874349 2304 66-67 0.65201685513005 2304 68-69 0.6680957586913365 2304 70-71 0.6747783723463172 2304 72-73 0.6477732987875484 2304 74-75 0.6637338605096303 2304 76-77 0.6729080855799836 2304 78-79 0.6930655115936375 2304 80-81 0.7078412395869762 2304 82-83 0.6904513464246449 2304 84-85 0.6978009864512842 2304 86-87 0.6977259225511361 2304 88-89 0.6876542181977428 2304 90-91 0.6791311645989637 2304 92-93 0.7099404119661301 2304 94-95 0.7261926300700665 2304 96-97 0.7099554487440898 2304 98-99 0.7587710602868967 2304 100-101 0.8265649368555259 2202 1 0.09714058818813243 2202 2 0.1167884244389441 2202 3 0.13707537055996255 2202 4 0.2032251044324056 2202 5 0.20148195773227684 2202 6 0.23683091348385688 2202 7 0.20419205623701941 2202 8 0.2413649360133192 2202 9 0.2128771496654167 2202 10-11 0.2415111234135452 2202 12-13 0.2509380363718918 2202 14-15 0.40708485448072906 2202 16-17 0.4067154206473944 2202 18-19 0.4355670790283668 2202 20-21 0.42911571121469905 2202 22-23 0.46483888199452394 2202 24-25 0.4793023818763018 2202 26-27 0.47561703321973425 2202 28-29 0.496745697304938 2202 30-31 0.5011835005893985 2202 32-33 0.5001515113831871 2202 34-35 0.4761115426279332 2202 36-37 0.4571877774827584 2202 38-39 0.5397570931676654 2202 40-41 0.5068110035121052 2202 42-43 0.5272317349628182 2202 44-45 0.5720568909889465 2202 46-47 0.535027056917059 2202 48-49 0.571500510290285 2202 50-51 0.5008362822161487 2202 52-53 0.5202993775730747 2202 54-55 0.5919689592379029 2202 56-57 0.5631258091074116 2202 58-59 0.5577405543861929 2202 60-61 0.5778014212223042 2202 62-63 0.57996068791185 2202 64-65 0.5823135581074936 2202 66-67 0.6014742164622007 2202 68-69 0.5668558297244743 2202 70-71 0.6058434851585304 2202 72-73 0.6439967666272608 2202 74-75 0.5949821908934183 2202 76-77 0.5986777971570731 2202 78-79 0.6213007780375861 2202 80-81 0.6087489302386473 2202 82-83 0.5728436057138353 2202 84-85 0.6140015386329836 2202 86-87 0.6361877860924068 2202 88-89 0.6581401501403761 2202 90-91 0.6530100858939321 2202 92-93 0.67654828861955 2202 94-95 0.6819328746116113 2202 96-97 0.6724763447853235 2202 98-99 0.6607854538460529 2202 100-101 0.7202974566053584 1102 1 0.034186666372654884 1102 2 0.11817103364274573 1102 3 0.11026670050164711 1102 4 0.18394578426780583 1102 5 0.1728047142793585 1102 6 0.20406200691319754 1102 7 0.19484694492299326 1102 8 0.1831845651999373 1102 9 0.21298978648003697 1102 10-11 0.1904171585701846 1102 12-13 0.20478304613368437 1102 14-15 0.3159935539667771 1102 16-17 0.3241023701268446 1102 18-19 0.3699517633632894 1102 20-21 0.3316024611934978 1102 22-23 0.34994618564486757 1102 24-25 0.342093847544092 1102 26-27 0.3809640978604065 1102 28-29 0.38395174243350993 1102 30-31 0.4004245808772495 1102 32-33 0.4038859806343993 1102 34-35 0.39131070737935403 1102 36-37 0.3535158038936217 1102 38-39 0.4111080444584658 1102 40-41 0.4252685680133581 1102 42-43 0.47361117944991094 1102 44-45 0.4164860484799604 1102 46-47 0.4538313888936898 1102 48-49 0.4858909511080114 1102 50-51 0.38859298284592825 1102 52-53 0.44560085847266606 1102 54-55 0.4813250130855451 1102 56-57 0.4792217420027569 1102 58-59 0.47321317893607073 1102 60-61 0.5263880099773814 1102 62-63 0.5409893798750502 1102 64-65 0.5309161790690595 1102 66-67 0.5494901801183403 1102 68-69 0.5128406227974409 1102 70-71 0.5228417690659981 1102 72-73 0.5519698501616688 1102 74-75 0.5417022966812439 1102 76-77 0.5798752763244508 1102 78-79 0.574176523523299 1102 80-81 0.5175488435499886 1102 82-83 0.5674772296662027 1102 84-85 0.5748235861166222 1102 86-87 0.5924676036108139 1102 88-89 0.5996712695710471 1102 90-91 0.6237407673747555 1102 92-93 0.5803287332801474 1102 94-95 0.6249903278215356 1102 96-97 0.6382138264794648 1102 98-99 0.5690730361574623 1102 100-101 0.5924808233397201 2305 1 0.12242616857825084 2305 2 0.08589846432002446 2305 3 0.12873256481063322 2305 4 0.13796010916777135 2305 5 0.14600544631670687 2305 6 0.16149905824833155 2305 7 0.1482138391152077 2305 8 0.19174885893954752 2305 9 0.17929792392991573 2305 10-11 0.1836763151839591 2305 12-13 0.15749100684506345 2305 14-15 0.26848373110564694 2305 16-17 0.2981238120431513 2305 18-19 0.31138360245008556 2305 20-21 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-0.990498099298172 1213 60-61 -0.9816594100570626 1213 62-63 -0.9972633737382282 1213 64-65 -1.0764749232723787 1213 66-67 -1.0399891619865187 1213 68-69 -1.1541988557526324 1213 70-71 -1.226142409373331 1213 72-73 -0.9426840971546113 1213 74-75 -0.9596788130672067 1213 76-77 -0.9194123076228138 1213 78-79 -0.9021765311127226 1213 80-81 -0.9701339994848865 1213 82-83 -0.970960473410095 1213 84-85 -1.0527667039035222 1213 86-87 -1.0140815553951938 1213 88-89 -1.0292957720933558 1213 90-91 -1.0417740026269726 1213 92-93 -1.0095763712074515 1213 94-95 -1.1329915266750632 1213 96-97 -1.177661469391353 1213 98-99 -1.0876476861725592 1213 100-101 -1.0679495695387153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15477.0 3 1538.0 4 1006.0 5 781.0 6 790.0 7 1471.0 8 2423.0 9 6704.0 10 27957.0 11 43792.0 12 29701.0 13 23170.0 14 22172.0 15 22282.0 16 23741.0 17 26432.0 18 29177.0 19 32762.0 20 36268.0 21 39743.0 22 44967.0 23 50369.0 24 57585.0 25 65841.0 26 77223.0 27 99616.0 28 111998.0 29 131174.0 30 162592.0 31 209272.0 32 275463.0 33 374899.0 34 529772.0 35 845544.0 36 1912027.0 37 5723371.0 38 6002121.0 39 6096.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.382689888323924 13.055565873710131 9.751072711536498 56.810671526429445 2 21.22639444672828 19.673587680300784 41.68800646196502 17.412011411005924 3 23.097216410203323 23.624397088006884 25.25513271443181 28.023253787357984 4 26.48757339131191 28.80034189083399 17.943353880778425 26.768730837075672 5 28.160049876359537 30.14493439115596 21.958432298322975 19.736583434161528 6 20.8108924020679 32.413630775859474 23.851059999862752 22.924416822209874 7 18.994969067714344 14.813230231580082 41.87908283001526 24.312717870690317 8 20.475634383488288 20.138188058932865 28.511786162776854 30.874391394801993 9 22.10630966163621 19.43372757783397 31.368081062382302 27.091881698147525 10-11 25.17913071400898 28.42441203122276 21.387398906102145 25.00905834866612 12-13 23.10379952476435 22.55287463556612 27.972048846110752 26.37127699355878 14-15 23.80412433735703 24.028482023604774 26.3789970157415 25.788396623296695 16-17 25.241226268737986 24.315032283200296 24.967212191338103 25.476529256723612 18-19 24.85922334454509 24.526754531426896 24.92343020704073 25.690591916987287 20-21 24.707723226723957 24.93034070603715 25.277841233119965 25.08409483411893 22-23 24.947109636079794 24.758992095313904 25.088227863000462 25.20567040560584 24-25 24.42520989208735 25.18509371967037 25.072874725176852 25.31682166306543 26-27 24.24903295625477 25.38122787876249 25.370922797310108 24.99881636767263 28-29 24.723955199227284 24.87750751217445 25.026231523459412 25.372305765138854 30-31 24.782916472731472 24.62930484448428 25.138675209521118 25.44910347326313 32-33 24.59242302946618 25.028409959037457 25.33952210504497 25.039644906451393 34-35 24.933216831622765 25.075714379572663 24.8023987854726 25.18867000333197 36-37 24.32482503383549 24.95191343535463 25.132277467991692 25.59098406281819 38-39 24.423038678797557 24.89777541036789 25.52090220218006 25.158283708654494 40-41 25.144485281819946 24.713992966164707 24.930418667538316 25.21110308447703 42-43 24.854251951853918 24.57646758973177 25.206432886195067 25.362847572219238 44-45 25.023464495637498 24.79378127872195 25.26968586630548 24.913068359335067 46-47 25.04944986955927 24.720782718906104 24.875832110382724 25.353935301151896 48-49 24.533685325162715 24.585097037001315 24.89318282814192 25.98803480969405 50-51 24.853262565840204 24.841546164718398 25.23007118073125 25.075120088710147 52-53 24.80593446405546 24.92971479259683 25.16715447366203 25.09719626968568 54-55 24.397769802269632 24.825483335179545 25.096748568278386 25.679998294272437 56-57 24.440867483906537 24.86113101814592 25.860846908464623 24.83715458948292 58-59 24.659612850668704 25.250314191452556 25.04676687456148 25.04330608331726 60-61 24.3798868839084 25.44942239861454 24.92629721088379 25.244393506593266 62-63 24.51907606652953 25.60492554317033 25.09382560222179 24.782172788078345 64-65 24.66076463256453 25.57690426067092 24.809754605027283 24.952576501737266 66-67 24.487180134039125 25.443139092726646 24.845510738301734 25.2241700349325 68-69 24.57070534399674 25.601366874032177 25.046134433314215 24.781793348656866 70-71 24.82069133832776 25.432078755641573 24.766254279549138 24.98097562648153 72-73 24.621960205472863 25.335911521827388 24.865041101612384 25.17708717108737 74-75 24.679492333462484 25.477702408550922 25.059984598025725 24.782820659960862 76-77 24.82934712801842 25.38709495625956 24.798779893604657 24.984778022117364 78-79 24.705370272979014 25.274282204004724 24.86191473654242 25.158432786473846 80-81 24.784348575115096 25.45482276578253 24.951051086263824 24.809777572838545 82-83 24.96757923508231 25.370182775325418 24.675636890718117 24.986601098874154 84-85 24.722469352554967 25.27596274812007 24.877640051323844 25.12392784800112 86-87 24.857779623614537 25.512454602334827 24.95527953979805 24.674486234252583 88-89 24.962837106392104 25.31210392005498 24.668924868988647 25.056134104564272 90-91 24.89789413848282 25.13737820874799 24.768501568024963 25.19622608474423 92-93 24.895091014676098 25.397988404574857 24.9564769033944 24.750443677354642 94-95 25.096315954164165 25.300499119291825 24.688131464183463 24.915053462360547 96-97 24.839033246274074 25.270092431417 24.76827621199834 25.122598110310584 98-99 25.000300636322443 25.40566105527749 24.88055058976047 24.7134877186396 100-101 25.300373625578214 25.318436956160745 24.501997289943173 24.879192128317865 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13637.0 1 7253.0 2 803.0 3 703.5 4 613.5 5 537.5 6 463.5 7 398.5 8 394.0 9 399.0 10 432.0 11 453.0 12 474.0 13 541.5 14 612.0 15 686.5 16 767.5 17 931.0 18 1154.5 19 1337.5 20 1745.5 21 2430.5 22 3266.0 23 4715.5 24 7065.5 25 10381.0 26 15028.0 27 21229.0 28 29378.0 29 40394.5 30 54033.0 31 71121.5 32 91782.5 33 117372.0 34 150062.0 35 190873.5 36 241357.5 37 302147.5 38 373303.5 39 449486.0 40 524578.5 41 596481.5 42 660129.5 43 702465.5 44 724494.5 45 727188.0 46 711229.5 47 683174.5 48 646295.5 49 609074.0 50 571033.0 51 534891.0 52 508962.5 53 491662.5 54 479856.5 55 470050.5 56 458352.0 57 446374.5 58 427997.5 59 408123.5 60 390517.5 61 367990.5 62 346926.5 63 321918.0 64 287217.5 65 257787.0 66 238909.5 67 220891.5 68 199805.0 69 175224.0 70 149400.5 71 123367.0 72 100686.0 73 83087.0 74 64688.0 75 47651.5 76 33972.5 77 23615.5 78 16365.5 79 11091.5 80 7322.0 81 4509.5 82 2756.0 83 1680.5 84 1076.0 85 754.5 86 582.5 87 467.5 88 372.5 89 293.5 90 243.0 91 209.0 92 166.5 93 128.0 94 101.5 95 77.5 96 47.0 97 28.0 98 14.0 99 7.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15325197276174105 2 0.14544172349995022 3 0.1551503379236467 4 0.15011146743216874 5 0.1569022242921954 6 0.10504287229211245 7 0.11007588363185614 8 0.10597447741786245 9 0.09406282194207795 10-11 0.08195781445906232 12-13 0.08997020445568568 14-15 0.13406910998371915 16-17 0.14294572486114834 18-19 0.13130359036514058 20-21 0.16273500984366787 22-23 0.143637104765793 24-25 0.1397788533487718 26-27 0.13082313992292988 28-29 0.12881931002980726 30-31 0.09300524505404101 32-33 0.10451554863602758 34-35 0.11927475185467054 36-37 0.1244220166532326 38-39 0.12320038351663593 40-41 0.15718346357544072 42-43 0.13781310794192198 44-45 0.1282187469770439 46-47 0.16162763016588957 48-49 0.1834822661347416 50-51 0.15879180072649965 52-53 0.108781011098581 54-55 0.11058270025687106 56-57 0.11321931853729558 58-59 0.11245176966010534 60-61 0.11696917564723266 62-63 0.0992159458923743 64-65 0.1163686125944693 66-67 0.13149694237237172 68-69 0.13062099918809733 70-71 0.11780703434523424 72-73 0.12747756428265788 74-75 0.1637222769108935 76-77 0.19181105032501594 78-79 0.1663295994326466 80-81 0.13934527612043535 82-83 0.12388004511781202 84-85 0.13017570365629233 86-87 0.12391227045235054 88-89 0.12745119809985367 90-91 0.14191451415591566 92-93 0.1090417433507563 94-95 0.11360016340002357 96-97 0.11449954319123502 98-99 0.11801503423180106 100-101 0.11112467179229166 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7067317E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.14560564919071 #Duplication Level Percentage of deduplicated Percentage of total 1 76.60702069378212 43.01147573832483 2 12.52354956263616 14.062845501437293 3 4.038901380362972 6.802996924734944 4 2.1547114873833575 4.839103258336285 5 1.2366193778132297 3.471537196242459 6 0.8312572382899773 2.800286465643865 7 0.5638043861240424 2.2158597108623175 8 0.3437482481629681 1.5439962867166523 9 0.24154311409181484 1.2205425987968863 >10 1.3524364842652552 13.254008091111805 >50 0.07152552317353261 2.7036207743963567 >100 0.033488348618901015 3.289112997254098 >500 0.0011581382722168861 0.419964296334151 >1k 2.0399372251922473E-4 0.18459598240174757 >5k 2.157931625740161E-5 0.09010289472030904 >10k+ 1.044398477575156E-5 0.08995128268595148 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.05151952119949492 4.68732138742135E-5 0.0 2 0.05190622521395718 4.68732138742135E-5 1.1718303468553375E-5 3 0.05373428055505151 5.2732365608490195E-5 1.1718303468553375E-5 4 0.060314107952644226 5.2732365608490195E-5 1.1718303468553375E-5 5 0.06145664254082818 5.2732365608490195E-5 1.7577455202830065E-5 6 0.06474362666375741 6.445066907704357E-5 1.7577455202830065E-5 7 0.0654467248718706 7.030982081132026E-5 1.7577455202830065E-5 8 0.06714001972307657 7.030982081132026E-5 1.7577455202830065E-5 9 0.06950125787199007 8.202812427987363E-5 1.7577455202830065E-5 10-11 0.07431748059756552 8.495770014701197E-5 1.7577455202830065E-5 12-13 0.079198153992218 9.3746427748427E-5 2.6366182804245094E-5 14-15 0.08397629223152064 1.0546473121698038E-4 2.929575867138344E-5 16-17 0.08900637399539718 1.201126105526721E-4 2.929575867138344E-5 18-19 0.09384896290377685 1.259717622869488E-4 2.929575867138344E-5 20-21 0.10203419787656139 1.3476048988836383E-4 2.929575867138344E-5 22-23 0.10765019481386559 1.464787933569172E-4 2.929575867138344E-5 24-25 0.1108903057229206 1.4940836922405554E-4 4.68732138742135E-5 26-27 0.11396636038341586 1.5819709682547058E-4 6.445066907704357E-5 28-29 0.11650337308435765 1.5819709682547058E-4 8.202812427987363E-5 30-31 0.11863317473976724 1.8163370376257732E-4 8.202812427987363E-5 32-33 0.12061063845008563 1.8163370376257732E-4 9.960557948270369E-5 34-35 0.1223742431221029 1.87492855496854E-4 1.259717622869488E-4 36-37 0.1241612844010573 1.933520072311307E-4 1.318309140212255E-4 38-39 0.1259190299213403 1.9921115896540738E-4 1.4061964162264052E-4 40-41 0.1276679867140219 2.0799988656682242E-4 1.611266726926089E-4 42-43 0.1291972253166681 2.314364935039292E-4 1.933520072311307E-4 44-45 0.13066787240197156 2.5487310044103594E-4 2.1092946243396078E-4 46-47 0.13215023779074356 2.6366182804245095E-4 2.1971819003537582E-4 48-49 0.13373220875899827 2.665914039095893E-4 2.4315479697248257E-4 50-51 0.13539034869979857 2.8123928324528104E-4 2.460843728396209E-4 52-53 0.13706899567166886 2.8416885911241934E-4 2.5487310044103594E-4 54-55 0.1386246004571193 2.9881673844811107E-4 2.578026763081743E-4 56-57 0.14014212075629695 3.046758901823878E-4 2.578026763081743E-4 58-59 0.14175924663495731 3.134646177838028E-4 2.6366182804245095E-4 60-61 0.1435843724001845 3.3690122472090954E-4 2.6366182804245095E-4 62-63 0.14576397684533543 3.5447867992373963E-4 2.753801315110043E-4 64-65 0.14802560941476625 3.57408255790878E-4 2.783097073781427E-4 66-67 0.15033997434980553 3.603378316580163E-4 2.870984349795577E-4 68-69 0.1528447617162088 3.6326740752515464E-4 2.929575867138344E-4 70-71 0.1558007037661514 3.7791528686084637E-4 2.929575867138344E-4 72-73 0.15932498353431884 3.8963359032939974E-4 2.9881673844811107E-4 74-75 0.16318323495134002 3.9842231793081476E-4 3.046758901823878E-4 76-77 0.16759224663138322 4.1599977313364485E-4 3.046758901823878E-4 78-79 0.17241432850869295 4.247885007350599E-4 3.046758901823878E-4 80-81 0.17870998704717328 4.394363800707516E-4 3.1639419365094116E-4 82-83 0.18745477101058122 4.5115468353930496E-4 3.5154910405660127E-4 84-85 0.19883031410267943 4.6873213874213505E-4 3.57408255790878E-4 86-87 0.21292743317534912 4.745912904764117E-4 3.66196983392293E-4 88-89 0.23052832498511627 4.8630959394496513E-4 3.8377443859512303E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAGCGT 12120 0.0 26.169912 8 GAGCGTC 12375 0.0 25.483007 9 TTAAAAA 23325 0.0 23.372099 56-57 AAGAGCG 15895 0.0 20.968903 7 ATCGGAA 17560 0.0 20.397003 2 TCGGGGG 11595 0.0 20.342861 38-39 CCGTATC 9635 0.0 19.973 48-49 TCGTGTA 9375 0.0 18.952522 14-15 CGCCGTA 10035 0.0 18.821987 46-47 TTTAAAA 14645 0.0 18.644724 56-57 TCGGAAG 19800 0.0 17.825298 3 GTAGGGA 10520 0.0 17.702547 18-19 GTGTAGG 11205 0.0 17.277716 16-17 GATCGGA 21180 0.0 17.0455 1 GCCGTAT 8550 0.0 17.005955 46-47 ATCGGGA 9305 0.0 16.897839 2 GGGGCGC 8120 0.0 16.64794 42-43 CGGAAGA 21200 0.0 16.625555 4 CATTAAA 14605 0.0 16.273296 54-55 CGGGAGA 10045 0.0 15.132411 4 >>END_MODULE