FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n02 k48-1.3420000000cac7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n02 k48-1.3420000000cac7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13484103
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC244250.18113922742951458Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC75900.026.6993948-49
TCGTGTA74250.025.24181414-15
CGCCGTA81450.024.47265246-47
GTAGGGA77950.024.07446318-19
AGAGCGT112850.022.4739729
TTAAAAA179950.022.11464156-57
ATCGGAA137500.021.0039393
AAGAGCG129500.019.8729178
AGCGTCG104050.019.81125610-11
CGTCGTG99450.019.72524812-13
AGTGTAG83500.019.6779128-29
AGATCGG130100.019.5340581
GTGTAGG98700.019.49481816-17
CGCGTAT22250.019.4279651
AGAGTGT85850.019.2253426-27
GCCGTAT68000.018.8659648-49
GAGTGTA66700.018.76048928-29
TGGTCGC98100.018.4537642-43
GATCGGA157300.018.3906232
TAGATCT115500.018.25752632-33
Produced by FastQC (version 0.11.2)