##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l06n02 k48-1.3420000000cac7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13484103 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40323238408962 33.0 33.0 33.0 33.0 33.0 2 32.36152504916345 33.0 33.0 33.0 33.0 33.0 3 32.346499133090276 33.0 33.0 33.0 33.0 33.0 4 36.20116755263587 37.0 37.0 37.0 37.0 37.0 5 36.19418065851321 37.0 37.0 37.0 37.0 37.0 6 36.23048029223746 37.0 37.0 37.0 37.0 37.0 7 36.24294630499337 37.0 37.0 37.0 37.0 37.0 8 36.263021796852186 37.0 37.0 37.0 37.0 37.0 9 36.22705544447413 37.0 37.0 37.0 37.0 37.0 10-11 36.21676554977368 37.0 37.0 37.0 37.0 37.0 12-13 36.2017459745005 37.0 37.0 37.0 37.0 37.0 14-15 38.50303909722434 40.0 40.0 40.0 37.0 40.0 16-17 38.48378868805734 40.0 40.0 40.0 37.0 40.0 18-19 38.45794395815577 40.0 40.0 40.0 37.0 40.0 20-21 38.42519283633476 40.0 40.0 40.0 37.0 40.0 22-23 38.450591596637906 40.0 40.0 40.0 37.0 40.0 24-25 38.37827803599542 40.0 40.0 40.0 37.0 40.0 26-27 38.33294298478735 40.0 38.5 40.0 37.0 40.0 28-29 38.274091498707776 40.0 37.0 40.0 37.0 40.0 30-31 38.21388597372773 40.0 37.0 40.0 37.0 40.0 32-33 38.14045780427516 40.0 37.0 40.0 37.0 40.0 34-35 38.087959354804696 40.0 37.0 40.0 37.0 40.0 36-37 38.02244413291711 40.0 37.0 40.0 37.0 40.0 38-39 37.93311423829972 40.0 37.0 40.0 35.0 40.0 40-41 37.837065098063995 40.0 37.0 40.0 33.0 40.0 42-43 37.731318612739756 40.0 37.0 40.0 33.0 40.0 44-45 37.65049161965019 40.0 37.0 40.0 33.0 40.0 46-47 37.493885948512855 40.0 37.0 40.0 33.0 40.0 48-49 37.37233307250767 40.0 37.0 40.0 33.0 40.0 50-51 35.94842678819644 38.5 35.0 38.5 30.0 40.0 52-53 36.29698186078822 37.0 37.0 40.0 33.0 40.0 54-55 36.98546577403035 37.0 37.0 40.0 33.0 40.0 56-57 36.96696858515542 37.0 37.0 40.0 33.0 40.0 58-59 36.817844724265306 37.0 37.0 40.0 33.0 40.0 60-61 36.68607422384714 37.0 37.0 40.0 33.0 40.0 62-63 36.52566392440046 37.0 37.0 40.0 33.0 40.0 64-65 36.33158119602023 37.0 37.0 40.0 33.0 40.0 66-67 36.07109694282222 37.0 37.0 40.0 33.0 40.0 68-69 35.82874359532852 37.0 37.0 37.0 33.0 40.0 70-71 35.609476840988236 37.0 37.0 37.0 33.0 40.0 72-73 35.38897789493302 37.0 37.0 37.0 33.0 40.0 74-75 35.19641781140355 37.0 37.0 37.0 33.0 38.5 76-77 35.00002799592973 37.0 37.0 37.0 33.0 37.0 78-79 34.841206382063376 37.0 35.0 37.0 33.0 37.0 80-81 34.72019399436507 37.0 33.0 37.0 33.0 37.0 82-83 34.62083903541823 37.0 33.0 37.0 33.0 37.0 84-85 34.51951379339063 37.0 33.0 37.0 30.0 37.0 86-87 34.4323576807445 37.0 33.0 37.0 27.0 37.0 88-89 34.34968473616673 37.0 33.0 37.0 27.0 37.0 90-91 34.25245928483341 37.0 33.0 37.0 27.0 37.0 92-93 34.199056029162634 37.0 33.0 37.0 27.0 37.0 94-95 34.13532038430736 37.0 33.0 37.0 27.0 37.0 96-97 34.07209504406782 37.0 33.0 37.0 27.0 37.0 98-99 33.996835087955056 37.0 33.0 37.0 27.0 37.0 100-101 32.30632697629201 35.0 30.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13317971761898661 2306 2 0.23020365124018838 2306 3 0.26674308479328346 2306 4 0.3460165360384906 2306 5 0.3516713565510088 2306 6 0.38574506858573443 2306 7 0.3594209913582489 2306 8 0.3435074639198419 2306 9 0.3647779519285166 2306 10-11 0.3902084822259937 2306 12-13 0.38235649061227406 2306 14-15 0.6328342116876442 2306 16-17 0.5889305550073232 2306 18-19 0.6309998942231303 2306 20-21 0.6697943045871213 2306 22-23 0.6303215141485268 2306 24-25 0.6234499751710345 2306 26-27 0.6787374815880867 2306 28-29 0.7136682668370895 2306 30-31 0.7069377249117892 2306 32-33 0.6838938778395587 2306 34-35 0.7254746674707064 2306 36-37 0.7261029061401132 2306 38-39 0.7207930249754284 2306 40-41 0.6976183610762519 2306 42-43 0.7254082370110666 2306 44-45 0.7133807886463543 2306 46-47 0.7081226170383275 2306 48-49 0.7370040422851645 2306 50-51 0.5829603783617401 2306 52-53 0.6332489818371769 2306 54-55 0.6986418747650021 2306 56-57 0.6357337611710889 2306 58-59 0.7027067993514464 2306 60-61 0.670325412828106 2306 62-63 0.640288650225834 2306 64-65 0.6745632761015941 2306 66-67 0.7060646103228478 2306 68-69 0.6787059212740587 2306 70-71 0.6755623776744955 2306 72-73 0.7436715870936865 2306 74-75 0.6991450132973895 2306 76-77 0.662214965448392 2306 78-79 0.6874951044833679 2306 80-81 0.7199749672101419 2306 82-83 0.7446875854778128 2306 84-85 0.7225654590875763 2306 86-87 0.7455675046484629 2306 88-89 0.7750879937601454 2306 90-91 0.8107898971802499 2306 92-93 0.7759531003826723 2306 94-95 0.7807035544164975 2306 96-97 0.793582293291685 2306 98-99 0.7904400075347198 2306 100-101 0.6776171524851335 2307 1 -0.08372996355510054 2307 2 0.1068175211488196 2307 3 0.21938289404843658 2307 4 0.2735466948515892 2307 5 0.291695065590865 2307 6 0.3087390215258523 2307 7 0.32926824560254886 2307 8 0.3133783000132695 2307 9 0.35474640175194594 2307 10-11 0.34783629086375356 2307 12-13 0.34557558661618515 2307 14-15 0.6218311993298542 2307 16-17 0.581179835434348 2307 18-19 0.6219749039944773 2307 20-21 0.6327576784690905 2307 22-23 0.6434222110506127 2307 24-25 0.6353978685659527 2307 26-27 0.6846139941694815 2307 28-29 0.6740940910931812 2307 30-31 0.6703814930300283 2307 32-33 0.6592548760736676 2307 34-35 0.718321709959632 2307 36-37 0.6848349193940777 2307 38-39 0.7025352681455388 2307 40-41 0.7600663288949363 2307 42-43 0.7527332320179667 2307 44-45 0.7773301571099935 2307 46-47 0.7196582062081376 2307 48-49 0.7867654823180033 2307 50-51 0.6976031769428417 2307 52-53 0.7118831561991712 2307 54-55 0.7441652766564317 2307 56-57 0.7368114791768576 2307 58-59 0.7405738176603833 2307 60-61 0.7437996396889375 2307 62-63 0.7020673971358775 2307 64-65 0.7241738318322746 2307 66-67 0.7636294681425184 2307 68-69 0.7904453164030656 2307 70-71 0.7455417555880643 2307 72-73 0.7857032510544002 2307 74-75 0.8292239196771121 2307 76-77 0.8153532936190473 2307 78-79 0.8562444534902696 2307 80-81 0.8518187309282652 2307 82-83 0.8230873507593444 2307 84-85 0.8593897454358554 2307 86-87 0.8418825571215223 2307 88-89 0.811988018751407 2307 90-91 0.8188882599605378 2307 92-93 0.8842129180461669 2307 94-95 0.8436571139866231 2307 96-97 0.8641137305435151 2307 98-99 0.8281686887352819 2307 100-101 0.7755354527219467 2201 1 0.0979918927649095 2201 2 0.1875188458529493 2201 3 0.19459788344790496 2201 4 0.24655062506946024 2201 5 0.24976819972599174 2201 6 0.272847507620277 2201 7 0.2932135815485779 2201 8 0.2707184261926514 2201 9 0.27349090813240906 2201 10-11 0.2786940904151365 2201 12-13 0.2829884631187838 2201 14-15 0.4805010901999296 2201 16-17 0.5027860458514226 2201 18-19 0.4524413251521864 2201 20-21 0.518615738499058 2201 22-23 0.5528248204557755 2201 24-25 0.5363807081562513 2201 26-27 0.508206567314545 2201 28-29 0.5295307234150783 2201 30-31 0.5683382749038799 2201 32-33 0.5660289370025069 2201 34-35 0.5486250167883355 2201 36-37 0.5862949018878467 2201 38-39 0.6202356895133576 2201 40-41 0.610623457924568 2201 42-43 0.62666482564088 2201 44-45 0.5650822303218419 2201 46-47 0.6143803039221609 2201 48-49 0.6121160710254046 2201 50-51 0.5587236584058459 2201 52-53 0.6502119559442079 2201 54-55 0.625097776825065 2201 56-57 0.6781793247484202 2201 58-59 0.669472676374447 2201 60-61 0.6432671329066011 2201 62-63 0.6737469054829717 2201 64-65 0.6564459742460329 2201 66-67 0.6609683942291227 2201 68-69 0.6434900302895414 2201 70-71 0.6996544546156329 2201 72-73 0.7154918132188968 2201 74-75 0.7076397496458569 2201 76-77 0.7059393624217307 2201 78-79 0.6539420878231468 2201 80-81 0.6856861886474874 2201 82-83 0.6743446915130775 2201 84-85 0.7121548889991018 2201 86-87 0.7430091614074286 2201 88-89 0.7213231874128709 2201 90-91 0.7594846920285434 2201 92-93 0.7503420961700229 2201 94-95 0.7319158323826755 2201 96-97 0.7418510553372784 2201 98-99 0.7317905432270706 2201 100-101 0.7014986976742321 1101 1 -0.013249052721938881 1101 2 0.1696796258116322 1101 3 0.1986567146957796 1101 4 0.28176101222756955 1101 5 0.31976451996474964 1101 6 0.35050301271547113 1101 7 0.3225358290664815 1101 8 0.3291203048311502 1101 9 0.351598723734071 1101 10-11 0.30372308608283305 1101 12-13 0.3272146420667852 1101 14-15 0.519744909756163 1101 16-17 0.5490558120773201 1101 18-19 0.5684626622262385 1101 20-21 0.5614666931915124 1101 22-23 0.6047893252676388 1101 24-25 0.609660794789157 1101 26-27 0.6223897742141133 1101 28-29 0.617803011465071 1101 30-31 0.6078938650510253 1101 32-33 0.575933369744007 1101 34-35 0.6171618247585045 1101 36-37 0.607452381375559 1101 38-39 0.6818396837937328 1101 40-41 0.687758616981931 1101 42-43 0.6342862599933383 1101 44-45 0.6064681895618023 1101 46-47 0.6721572502962445 1101 48-49 0.678007636902052 1101 50-51 0.5906128648441111 1101 52-53 0.6391809027958004 1101 54-55 0.7045792053449418 1101 56-57 0.6914225585893234 1101 58-59 0.684496206130838 1101 60-61 0.6501490619542238 1101 62-63 0.6541728953156181 1101 64-65 0.679041357852654 1101 66-67 0.6989313570954891 1101 68-69 0.6975185191278825 1101 70-71 0.6555772895946674 1101 72-73 0.7051633089476468 1101 74-75 0.7361109792002267 1101 76-77 0.7262653686563922 1101 78-79 0.7587559071251562 1101 80-81 0.7676423183306511 1101 82-83 0.7728982391916119 1101 84-85 0.801937794214588 1101 86-87 0.8026300747067125 1101 88-89 0.7140638286757479 1101 90-91 0.7326029366344002 1101 92-93 0.8061708835323742 1101 94-95 0.7715272513113831 1101 96-97 0.7706918839453678 1101 98-99 0.740982440277584 1101 100-101 0.7339969838314957 2304 1 -0.04415224651360461 2304 2 0.11583220837913899 2304 3 0.17456494882472384 2304 4 0.20439960234880772 2304 5 0.23355822297084217 2304 6 0.281264015879529 2304 7 0.23099361850736955 2304 8 0.2586286308073795 2304 9 0.20182650947167957 2304 10-11 0.25599443970569524 2304 12-13 0.26567854760862275 2304 14-15 0.45007751141011454 2304 16-17 0.47684694136959394 2304 18-19 0.4788490331954094 2304 20-21 0.4860155800033894 2304 22-23 0.5023227704255007 2304 24-25 0.5244181682013931 2304 26-27 0.4672490275626586 2304 28-29 0.5242827165245743 2304 30-31 0.49423431374767546 2304 32-33 0.5093229641162154 2304 34-35 0.5061577087260574 2304 36-37 0.5396601108532693 2304 38-39 0.5677411477299827 2304 40-41 0.5627563542475755 2304 42-43 0.5902791269626775 2304 44-45 0.5846057902263269 2304 46-47 0.5462448166009395 2304 48-49 0.586913141856023 2304 50-51 0.5470327496679346 2304 52-53 0.5317385622130786 2304 54-55 0.5777575212199082 2304 56-57 0.5620404768954401 2304 58-59 0.5882674739041747 2304 60-61 0.605245645977206 2304 62-63 0.6466282614151737 2304 64-65 0.6468857555996763 2304 66-67 0.6870954696310605 2304 68-69 0.6684093263428537 2304 70-71 0.6908251163262236 2304 72-73 0.6828750638626744 2304 74-75 0.7086752664423344 2304 76-77 0.7403278038868777 2304 78-79 0.7229048236913513 2304 80-81 0.7184167795468781 2304 82-83 0.7284699030906765 2304 84-85 0.7242014422478249 2304 86-87 0.7150502079024363 2304 88-89 0.7082310569522292 2304 90-91 0.7196966314471993 2304 92-93 0.7571371125579063 2304 94-95 0.7031036850487737 2304 96-97 0.6848640968126674 2304 98-99 0.7533125958342097 2304 100-101 0.7898017879867609 2202 1 0.12418630890214644 2202 2 0.11472663708630648 2202 3 0.1258033337097686 2202 4 0.15988129366056114 2202 5 0.18637407298157171 2202 6 0.23769846622264623 2202 7 0.17560859366042791 2202 8 0.2578596352489555 2202 9 0.24282445428133315 2202 10-11 0.20837831846068156 2202 12-13 0.21011603924468858 2202 14-15 0.3945816154360884 2202 16-17 0.39204099574653384 2202 18-19 0.40736279938217734 2202 20-21 0.38534893277468285 2202 22-23 0.44143986842795613 2202 24-25 0.45347402915718504 2202 26-27 0.39792721047236057 2202 28-29 0.43382054914729906 2202 30-31 0.5107932118845468 2202 32-33 0.4813598902572025 2202 34-35 0.4789454634100494 2202 36-37 0.4515245176329259 2202 38-39 0.48357201954485873 2202 40-41 0.5093028644237236 2202 42-43 0.4895794352038507 2202 44-45 0.5003265385279363 2202 46-47 0.4671261934663278 2202 48-49 0.49796578956768656 2202 50-51 0.4384515636683517 2202 52-53 0.44762670124532633 2202 54-55 0.509514809893787 2202 56-57 0.5396341921547574 2202 58-59 0.527152886388869 2202 60-61 0.5796581897406412 2202 62-63 0.578356318389325 2202 64-65 0.5662512707167977 2202 66-67 0.563564698968321 2202 68-69 0.5546516623668509 2202 70-71 0.5598808576638064 2202 72-73 0.5576566297995882 2202 74-75 0.5481538357188498 2202 76-77 0.5358399529849933 2202 78-79 0.5852671786449264 2202 80-81 0.5708830453430167 2202 82-83 0.5802678769077474 2202 84-85 0.6237532477725338 2202 86-87 0.6330289741751898 2202 88-89 0.6628415538986019 2202 90-91 0.6640790987073188 2202 92-93 0.6703632231195229 2202 94-95 0.6342871801283536 2202 96-97 0.6105539761403165 2202 98-99 0.6426105789964538 2202 100-101 0.7365270526233587 1102 1 0.0405919947303488 1102 2 0.05442129124750039 1102 3 0.03773366855097038 1102 4 0.08684638094018737 1102 5 0.11925983637230075 1102 6 0.16718721033511486 1102 7 0.13808305961227063 1102 8 0.14268341656164552 1102 9 0.1544375789345267 1102 10-11 0.11834818283950455 1102 12-13 0.1605944242646018 1102 14-15 0.2721914020433118 1102 16-17 0.28994302252930737 1102 18-19 0.3207526800156799 1102 20-21 0.27309949310821224 1102 22-23 0.3509932610481883 1102 24-25 0.3046751856263441 1102 26-27 0.35156031897540174 1102 28-29 0.3281302783491924 1102 30-31 0.37333734386628237 1102 32-33 0.3360117473937123 1102 34-35 0.3536720912083453 1102 36-37 0.34393923935645176 1102 38-39 0.38559882408885215 1102 40-41 0.415939495645425 1102 42-43 0.47504589054224056 1102 44-45 0.41109650604470716 1102 46-47 0.4423028085030367 1102 48-49 0.43616485627373436 1102 50-51 0.341107300673535 1102 52-53 0.39319196518354005 1102 54-55 0.4538888478753904 1102 56-57 0.4461053366114811 1102 58-59 0.44569698613999975 1102 60-61 0.5214467665152682 1102 62-63 0.5542825129036757 1102 64-65 0.545619825139056 1102 66-67 0.5594861982666544 1102 68-69 0.5061493840111098 1102 70-71 0.5118510190869046 1102 72-73 0.5519601959860196 1102 74-75 0.545884147388854 1102 76-77 0.5717099007278463 1102 78-79 0.5731819844293611 1102 80-81 0.5242408434522119 1102 82-83 0.5395203706423715 1102 84-85 0.5386764343869075 1102 86-87 0.5595549193814051 1102 88-89 0.5733905021789241 1102 90-91 0.6151130479937592 1102 92-93 0.5506734558158257 1102 94-95 0.6482867637437053 1102 96-97 0.6618907842819439 1102 98-99 0.5937875842848896 1102 100-101 0.5182787340594857 2305 1 0.09205727985035139 2305 2 0.10598960386163725 2305 3 0.1330517635975781 2305 4 0.12462465859209715 2305 5 0.16329203902051148 2305 6 0.18395541892270728 2305 7 0.1730368619277982 2305 8 0.21319937513023746 2305 9 0.17737591682318765 2305 10-11 0.183706877743397 2305 12-13 0.1864739122242014 2305 14-15 0.29967071576156457 2305 16-17 0.3351770291889409 2305 18-19 0.3775348043395823 2305 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-1.0433147333709343 1213 76-77 -1.0328753561302833 1213 78-79 -0.9689628279395137 1213 80-81 -0.9924328011109651 1213 82-83 -0.9945556292963289 1213 84-85 -1.0824447482888857 1213 86-87 -1.0461490443431813 1213 88-89 -1.071043731157033 1213 90-91 -1.1073416832994454 1213 92-93 -1.0744432382333144 1213 94-95 -1.1722247725795754 1213 96-97 -1.2243916341532852 1213 98-99 -1.1256874116494302 1213 100-101 -1.0799123505041521 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12377.0 3 1372.0 4 814.0 5 670.0 6 601.0 7 816.0 8 1467.0 9 4291.0 10 16668.0 11 27821.0 12 21793.0 13 18664.0 14 18632.0 15 19194.0 16 20757.0 17 23246.0 18 26116.0 19 28661.0 20 31679.0 21 35706.0 22 39163.0 23 44451.0 24 49808.0 25 56622.0 26 65917.0 27 85657.0 28 95258.0 29 106896.0 30 131732.0 31 168012.0 32 222778.0 33 304213.0 34 429679.0 35 680298.0 36 1536562.0 37 4489049.0 38 4661762.0 39 4901.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.55858891731902 13.235993637090745 14.26870367311237 50.93671377247786 2 23.479605638376277 18.777170887685013 37.04825480580231 20.694968668136404 3 25.08901938512336 23.47185926729347 25.385867038932027 26.05325430865114 4 26.936751007767413 27.736606973085003 18.61057781438065 26.71606420476693 5 28.884635125930448 29.88196179189889 21.382809697093947 19.85059338507672 6 21.775616577562815 33.294438559620886 22.067019280938904 22.8629255818774 7 19.885706572774136 15.820933021387754 40.94298351119339 23.350376894644718 8 21.350483384576254 21.282136393599675 27.13806144146521 30.22931878035886 9 22.175726409439378 20.700663822332416 30.315906682577122 26.80770308565108 10-11 25.25558210253567 28.741524697227117 21.10575099625398 24.897142203983236 12-13 23.458868768135336 22.94050078903707 27.62217641212843 25.97845403069916 14-15 23.89077051049446 24.567404230815473 26.437041828934717 25.10478342975535 16-17 24.967123392158136 24.620515768275812 25.302932137029455 25.109428702536597 18-19 24.71915323163057 25.03942344158866 25.300819331807663 24.940603994973106 20-21 24.53597008148789 25.242875455219693 25.460965654090085 24.760188809202333 22-23 24.82936942228896 24.989901961479692 25.26684649351206 24.91388212271929 24-25 24.409720364237568 25.374741450540217 25.274465051740343 24.941073133481872 26-27 24.213676186682523 25.641886495437525 25.310259898801796 24.834177419078156 28-29 24.662949322776043 25.244447533629323 25.034939906257346 25.057663237337284 30-31 24.645584132525926 25.052417698247385 25.288084388532067 25.01391378069462 32-33 24.584408831418123 25.229325343466474 25.329331074836848 24.85693475027855 34-35 24.68724262357113 25.302996934575265 24.969211356298228 25.04054908555538 36-37 24.20021974564588 25.26705875543233 25.332675029545516 25.200046469376268 38-39 24.35211493874855 25.130940709182553 25.516603706292873 25.000340645776024 40-41 24.98813921673742 24.96525330896726 25.01199448866338 25.034612985631945 42-43 24.693523937954772 24.97361137300251 25.394511180437757 24.938353508604962 44-45 24.994578187840833 25.022740793714686 25.242119510663493 24.740561507780992 46-47 24.805319984122445 24.906877955383205 25.107757936280215 25.180044124214128 48-49 24.383292268646787 24.969033380051055 25.0851131601392 25.562561191162956 50-51 24.56534260491515 25.113547461591008 25.340675811671957 24.980434121821883 52-53 24.61823518751191 25.147365178541005 25.34095656492335 24.89344306902374 54-55 24.24923052068002 25.095928768369795 25.34862298542533 25.306217725524853 56-57 24.260746169059757 25.12278220323961 25.836842921008984 24.77962870669165 58-59 24.54216642493148 25.314741441108 25.235783687905084 24.907308446055435 60-61 24.287515282591887 25.52583402151094 25.177239455661287 25.009411240235885 62-63 24.404375720441774 25.673841683668364 25.21630374001496 24.705478855874897 64-65 24.471902144259484 25.683430527086387 24.926315315837407 24.918352012816722 66-67 24.38523931245452 25.585516515716588 25.001587339189545 25.02765683263935 68-69 24.487931744505804 25.663695850264645 25.092295985946585 24.756076419282966 70-71 24.634215358689215 25.505001585630467 24.93605688043708 24.924726175243244 72-73 24.49484745536916 25.532191861412556 25.09284241345089 24.880118269767394 74-75 24.678374202514437 25.515571076658528 25.092272207711325 24.71378251311571 76-77 24.82826085906538 25.405043110798857 24.926170876070433 24.84052515406533 78-79 24.60201447600981 25.385211743884334 25.04536753422883 24.96740624587703 80-81 24.738146084751747 25.49224258263457 24.960380189760677 24.809231142853005 82-83 24.783521829223417 25.431895415197936 24.847032612189295 24.93755014338935 84-85 24.589844614438196 25.35487218437744 25.037679758588148 25.017603442596215 86-87 24.703074833922585 25.560519752468768 25.04379320170899 24.692612211899654 88-89 24.87800942359868 25.427331347097574 24.84426646054548 24.850392768758265 90-91 24.7933085749239 25.302654770509392 24.950997124590295 24.95303952997642 92-93 24.770259571575878 25.527795760349857 25.085894081914184 24.61605058616008 94-95 24.861988881447182 25.36546778662735 24.917578304369652 24.85496502755582 96-97 24.656661520496804 25.453612487246875 24.951298492775873 24.938427499480444 98-99 24.905788271479764 25.386649923525017 24.99403948810359 24.713522316891627 100-101 25.05060970720393 25.348161226423223 24.78833240773464 24.812896658638213 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10815.0 1 5781.5 2 676.0 3 596.0 4 533.0 5 462.0 6 409.0 7 374.0 8 363.0 9 357.5 10 383.5 11 418.0 12 438.5 13 459.5 14 523.0 15 590.5 16 693.0 17 859.0 18 1034.5 19 1259.0 20 1650.5 21 2268.0 22 3178.0 23 4485.5 24 6483.5 25 9492.5 26 13546.5 27 19004.0 28 26258.5 29 35593.0 30 47122.5 31 61578.5 32 79054.0 33 100847.5 34 127937.5 35 162073.0 36 204601.5 37 257011.5 38 315123.5 39 372082.0 40 429024.0 41 482957.0 42 527839.0 43 559841.5 44 579112.0 45 577985.0 46 560369.0 47 542009.5 48 514171.0 49 479290.5 50 452549.5 51 424006.5 52 395879.0 53 376251.5 54 362533.5 55 352778.5 56 347349.5 57 343203.0 58 328381.0 59 303574.0 60 285257.5 61 271761.5 62 257181.5 63 240548.5 64 213787.5 65 192259.5 66 183152.5 67 173122.5 68 158343.5 69 138477.5 70 118369.5 71 99020.5 72 80533.5 73 67005.0 74 54660.5 75 41888.0 76 29919.5 77 20457.5 78 14506.5 79 11656.5 80 8767.5 81 5589.0 82 2977.5 83 1270.0 84 772.5 85 556.0 86 457.0 87 361.0 88 281.0 89 230.5 90 181.0 91 161.0 92 142.5 93 104.5 94 79.0 95 59.5 96 35.5 97 25.5 98 18.0 99 6.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15632482190324415 2 0.14842663245749457 3 0.15800086961661447 4 0.15335836577338516 5 0.15954342680414116 6 0.10699265646368913 7 0.11188730907795646 8 0.10827564873985315 9 0.0955050551008102 10-11 0.08256389023430034 12-13 0.09123706634397558 14-15 0.13699094407688817 16-17 0.1464131503593528 18-19 0.13445462408585873 20-21 0.16664808923515342 22-23 0.1472437580757133 24-25 0.14327612300202688 26-27 0.13389099742118551 28-29 0.13176627321817402 30-31 0.09452983264811905 32-33 0.10660701716680747 34-35 0.12218091184856716 36-37 0.12733142130403483 38-39 0.12633024236020743 40-41 0.1607077608351108 42-43 0.14106982125544426 44-45 0.13100241076473534 46-47 0.16562836994051439 48-49 0.18730204003929662 50-51 0.16234672784685789 52-53 0.11133109855360791 54-55 0.11313322065249723 56-57 0.11599214274764884 58-59 0.11500950415463304 60-61 0.11988190834792643 62-63 0.10112278139673064 64-65 0.1192033315082212 66-67 0.13476610197949393 68-69 0.13387245707037393 70-71 0.12069026764331302 72-73 0.13053519392428253 74-75 0.16741195168859213 76-77 0.1953263038705652 78-79 0.17008917834578985 80-81 0.1427458689688146 82-83 0.12680487534098486 84-85 0.13339411601943416 86-87 0.12693094972650387 88-89 0.13046103252103608 90-91 0.14525994053887012 92-93 0.11156470697383429 94-95 0.11653352099134812 96-97 0.11742716590046813 98-99 0.12088308729175386 100-101 0.11378584100106622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3484103E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.602250638608 #Duplication Level Percentage of deduplicated Percentage of total 1 78.40207691496173 46.72940238872971 2 12.732251657134672 15.177417089247456 3 3.9618770772373018 7.0841037167056005 4 1.742920946129277 4.155280442979078 5 0.9921036791989765 2.9565806073551273 6 0.529419692376466 1.8932763118822125 7 0.3085502796676053 1.2873203772352841 8 0.17398274630860625 0.8295810601823119 9 0.17086034714072226 0.9165295111032808 >10 0.8734500975959675 9.552301386646045 >50 0.07031116862817542 2.8899394920983537 >100 0.03847104134872378 4.352448098661931 >500 0.0028875273555710574 1.1861993605519827 >1k 8.115627471286228E-4 0.7599610524689914 >5k 1.2775484446053714E-5 0.047879826721831886 >10k+ 1.2486684568533256E-5 0.18177927743077046 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 24425 0.18113922742951458 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.19288639370375618 1.4832280649294951E-5 0.0 2 0.19307179721187237 1.4832280649294951E-5 1.4832280649294951E-5 3 0.19338327510550757 1.4832280649294951E-5 1.4832280649294951E-5 4 0.1946959319429702 1.4832280649294951E-5 2.2248420973942427E-5 5 0.19500740983660536 2.2248420973942427E-5 2.9664561298589902E-5 6 0.1958231852723166 2.2248420973942427E-5 2.9664561298589902E-5 7 0.19600858878043279 2.2248420973942427E-5 3.708070162323738E-5 8 0.19646838948056092 2.9664561298589902E-5 4.449684194788485E-5 9 0.19700976772426018 5.191298227253233E-5 5.9329122597179805E-5 10-11 0.19822230666734006 5.9329122597179805E-5 5.9329122597179805E-5 12-13 0.19954979578545196 6.674526292182728E-5 5.9329122597179805E-5 14-15 0.20097369472778426 8.528561373344598E-5 5.9329122597179805E-5 16-17 0.2024643389330384 1.0382596454506466E-4 6.303719275950354E-5 18-19 0.20386598945439677 1.0753403470738839E-4 7.045333308415102E-5 20-21 0.20562361471133822 1.2236631535668334E-4 8.89936838957697E-5 22-23 0.2068398617245804 1.3349052584365456E-4 8.89936838957697E-5 24-25 0.20811914593058212 1.3349052584365456E-4 1.0382596454506466E-4 26-27 0.20925381540025317 1.483228064929495E-4 1.1124210486971213E-4 28-29 0.2103328638174894 1.483228064929495E-4 1.1124210486971213E-4 30-31 0.21131550241050517 1.668631573045682E-4 1.1124210486971213E-4 32-33 0.21225364416157308 1.965277186031581E-4 1.3349052584365456E-4 34-35 0.21329931994734838 2.224842097394243E-4 1.483228064929495E-4 36-37 0.21423375362825395 2.5956491136266166E-4 1.668631573045682E-4 38-39 0.2152683052035423 2.5956491136266166E-4 1.7057122746689195E-4 40-41 0.21681457046123126 2.669810516873091E-4 1.8540350811618688E-4 42-43 0.21842016484151744 2.818133323366041E-4 2.1877613957710054E-4 44-45 0.21935830659258537 2.96645612985899E-4 2.2248420973942427E-4 46-47 0.22051151641306804 2.96645612985899E-4 2.2248420973942427E-4 48-49 0.22191316693442642 3.1889403395984146E-4 2.2248420973942427E-4 50-51 0.22340381113968055 3.2260210412216517E-4 2.2990035006407176E-4 52-53 0.22478321324006498 3.300182444468127E-4 2.4473263071336673E-4 54-55 0.226192279901748 3.522666654207551E-4 2.521487710380142E-4 56-57 0.22765325954570356 3.7822315655702127E-4 2.6327298152498536E-4 58-59 0.22915502796144466 3.9676350736863995E-4 2.706891218496329E-4 60-61 0.2312055907612097 4.041796476932874E-4 2.743971920119566E-4 62-63 0.23344526513925323 4.486764896411723E-4 2.7810526217428034E-4 64-65 0.23572202021892003 4.67216840452791E-4 2.96645612985899E-4 66-67 0.2381396819647551 4.7092491061511473E-4 3.263101742844889E-4 68-69 0.24072791493805706 4.820491211020859E-4 3.448505250961076E-4 70-71 0.24339401738476785 5.005894719137046E-4 3.6709894607005005E-4 72-73 0.24664599491712574 5.042975420760284E-4 3.7451508639469756E-4 74-75 0.25008337595759983 5.191298227253233E-4 3.7822315655702127E-4 76-77 0.25391010436511796 5.487943840239132E-4 3.7822315655702127E-4 78-79 0.2585414839978603 5.487943840239132E-4 3.893473670439925E-4 80-81 0.26396639064533994 5.599185945108845E-4 4.004715775309637E-4 82-83 0.2709264383400216 5.636266646732082E-4 4.301361388295536E-4 84-85 0.2805377562007647 5.636266646732082E-4 4.67216840452791E-4 86-87 0.29145060668848344 5.710428049978556E-4 4.7463298077743844E-4 88-89 0.3041433308541176 6.192477171080642E-4 4.820491211020859E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTATC 7590 0.0 26.69939 48-49 TCGTGTA 7425 0.0 25.241814 14-15 CGCCGTA 8145 0.0 24.472652 46-47 GTAGGGA 7795 0.0 24.074463 18-19 AGAGCGT 11285 0.0 22.473972 9 TTAAAAA 17995 0.0 22.114641 56-57 ATCGGAA 13750 0.0 21.003939 3 AAGAGCG 12950 0.0 19.872917 8 AGCGTCG 10405 0.0 19.811256 10-11 CGTCGTG 9945 0.0 19.725248 12-13 AGTGTAG 8350 0.0 19.67791 28-29 AGATCGG 13010 0.0 19.534058 1 GTGTAGG 9870 0.0 19.494818 16-17 CGCGTAT 2225 0.0 19.427965 1 AGAGTGT 8585 0.0 19.22534 26-27 GCCGTAT 6800 0.0 18.86596 48-49 GAGTGTA 6670 0.0 18.760489 28-29 TGGTCGC 9810 0.0 18.45376 42-43 GATCGGA 15730 0.0 18.390623 2 TAGATCT 11550 0.0 18.257526 32-33 >>END_MODULE