##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	C5B16ACXX l06n01 mx48-1.3410000000cc1b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12373852
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80112692474421	33.0	33.0	33.0	33.0	33.0
2	32.80689319704163	33.0	33.0	33.0	33.0	33.0
3	32.7221204035736	33.0	33.0	33.0	33.0	33.0
4	36.723342496742326	37.0	37.0	37.0	37.0	37.0
5	36.74317940767354	37.0	37.0	37.0	37.0	37.0
6	36.69611483958269	37.0	37.0	37.0	37.0	37.0
7	36.698938374242715	37.0	37.0	37.0	37.0	37.0
8	36.70606089356815	37.0	37.0	37.0	37.0	37.0
9	36.705777392520936	37.0	37.0	37.0	37.0	37.0
10-11	36.68675518343035	37.0	37.0	37.0	37.0	37.0
12-13	36.68494790466218	37.0	37.0	37.0	37.0	37.0
14-15	39.122339025874886	40.0	40.0	40.0	37.0	40.0
16-17	39.11172103076714	40.0	40.0	40.0	37.0	40.0
18-19	39.071944411489646	40.0	40.0	40.0	37.0	40.0
20-21	39.07929580053164	40.0	40.0	40.0	37.0	40.0
22-23	39.04468798398429	40.0	40.0	40.0	37.0	40.0
24-25	39.017333850445276	40.0	40.0	40.0	37.0	40.0
26-27	38.966555483288474	40.0	40.0	40.0	37.0	40.0
28-29	38.91913670051977	40.0	40.0	40.0	37.0	40.0
30-31	38.858868321683495	40.0	40.0	40.0	37.0	40.0
32-33	38.79482399660186	40.0	40.0	40.0	37.0	40.0
34-35	38.711102048093025	40.0	38.5	40.0	37.0	40.0
36-37	38.65311085020251	40.0	37.0	40.0	37.0	40.0
38-39	38.56705082620998	40.0	37.0	40.0	37.0	40.0
40-41	38.471173002554096	40.0	37.0	40.0	37.0	40.0
42-43	38.32659421657864	40.0	37.0	40.0	37.0	40.0
44-45	38.21702053653139	40.0	37.0	40.0	37.0	40.0
46-47	38.13155523437649	40.0	37.0	40.0	37.0	40.0
48-49	37.999606710990236	40.0	37.0	40.0	37.0	40.0
50-51	37.88399594564409	40.0	37.0	40.0	33.0	40.0
52-53	37.72451100918292	40.0	37.0	40.0	33.0	40.0
54-55	37.52189447554407	38.5	37.0	40.0	33.0	40.0
56-57	37.36066723603935	37.0	37.0	40.0	33.0	40.0
58-59	37.20963241680926	37.0	37.0	40.0	33.0	40.0
60-61	37.016496681874	37.0	37.0	40.0	33.0	40.0
62-63	36.81890663473266	37.0	37.0	40.0	33.0	40.0
64-65	36.64224943857418	37.0	37.0	40.0	33.0	40.0
66-67	36.34165727050881	37.0	37.0	40.0	33.0	40.0
68-69	36.17104633221732	37.0	37.0	37.0	33.0	40.0
70-71	35.947038642453464	37.0	37.0	37.0	33.0	40.0
72-73	35.582497390464994	37.0	37.0	37.0	33.0	40.0
74-75	35.27053398569823	37.0	37.0	37.0	33.0	38.5
76-77	32.69228212039388	33.0	33.0	37.0	27.0	37.0
78-79	34.72518800127882	37.0	33.0	37.0	33.0	37.0
80-81	34.90576576315928	37.0	33.0	37.0	33.0	37.0
82-83	34.85624367416064	37.0	33.0	37.0	33.0	37.0
84-85	34.76146461910163	37.0	33.0	37.0	33.0	37.0
86-87	34.69924583710877	37.0	33.0	37.0	33.0	37.0
88-89	34.62354152126598	37.0	33.0	37.0	33.0	37.0
90-91	34.54862883441632	37.0	33.0	37.0	33.0	37.0
92-93	34.47731405709395	37.0	33.0	37.0	33.0	37.0
94-95	34.41633361220096	37.0	33.0	37.0	30.0	37.0
96-97	34.35453886954524	37.0	33.0	37.0	27.0	37.0
98-99	34.30780831223777	37.0	33.0	37.0	27.0	37.0
100-101	32.59600227964582	35.0	30.0	37.0	24.5	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2306	1	0.004128049811377821
2306	2	0.03471936794948505
2306	3	0.14558085704041446
2306	4	0.1686891144742333
2306	5	0.13646611937134878
2306	6	0.12331261304186825
2306	7	0.13325350964392868
2306	8	0.15078659855021215
2306	9	0.13417283783991252
2306	10-11	0.14269534951908014
2306	12-13	0.14521365399659913
2306	14-15	0.3367681293948763
2306	16-17	0.3603770495994567
2306	18-19	0.3790698013013625
2306	20-21	0.34362715497353946
2306	22-23	0.33382279686890115
2306	24-25	0.3504846121682448
2306	26-27	0.36278411661021437
2306	28-29	0.3776090925587994
2306	30-31	0.38665856691392975
2306	32-33	0.3778064112314894
2306	34-35	0.39963348375540164
2306	36-37	0.36411945732038475
2306	38-39	0.40224302994970174
2306	40-41	0.4312077671828689
2306	42-43	0.4052103688151547
2306	44-45	0.40938597095401974
2306	46-47	0.41844744478105156
2306	48-49	0.39890393718265926
2306	50-51	0.38702304809823573
2306	52-53	0.38400007481953224
2306	54-55	0.39704812422296243
2306	56-57	0.4171938239415667
2306	58-59	0.42863655649282606
2306	60-61	0.3876705065532917
2306	62-63	0.42415371608122854
2306	64-65	0.35511691474554397
2306	66-67	0.4541578904385517
2306	68-69	0.43606292310418127
2306	70-71	0.43421491317972993
2306	72-73	0.33246890704537435
2306	74-75	0.3928816403559452
2306	76-77	0.09890012178855301
2306	78-79	0.4351994242832049
2306	80-81	0.5154499251079656
2306	82-83	0.5534157656974728
2306	84-85	0.5822807919609332
2306	86-87	0.46878624958954873
2306	88-89	0.4335925587592371
2306	90-91	0.4699876387059163
2306	92-93	0.46579505031507296
2306	94-95	0.48365875195066366
2306	96-97	0.4347246683864512
2306	98-99	0.4525917970800677
2306	100-101	0.6855496796431098
2307	1	0.044196281368698465
2307	2	0.07168216390090976
2307	3	0.1692906598018311
2307	4	0.1506959851051306
2307	5	0.14049968410638058
2307	6	0.16413370951296002
2307	7	0.1634554480611783
2307	8	0.18069647140487888
2307	9	0.18068921278135974
2307	10-11	0.17048408793288417
2307	12-13	0.15990285388352987
2307	14-15	0.36885707090495856
2307	16-17	0.3637848025013781
2307	18-19	0.4016364373095769
2307	20-21	0.3895388538599249
2307	22-23	0.3706285723368623
2307	24-25	0.4038754637037556
2307	26-27	0.41216068371827674
2307	28-29	0.428433071746845
2307	30-31	0.3822236193454742
2307	32-33	0.39585401265239284
2307	34-35	0.4140732420715665
2307	36-37	0.4577545613288265
2307	38-39	0.4515503227053159
2307	40-41	0.44949487977955727
2307	42-43	0.45744073241792194
2307	44-45	0.45821638706970447
2307	46-47	0.48451347112727916
2307	48-49	0.46053859214652704
2307	50-51	0.49144627199577684
2307	52-53	0.5027695579072287
2307	54-55	0.5091083505136567
2307	56-57	0.48879494671887613
2307	58-59	0.4724511072994986
2307	60-61	0.41036782365703317
2307	62-63	0.45280080134963185
2307	64-65	0.4889252968903648
2307	66-67	0.4605484727753648
2307	68-69	0.4948013337994013
2307	70-71	0.4540073513394347
2307	72-73	0.47220902093809514
2307	74-75	0.47896483446852756
2307	76-77	0.28416496013613113
2307	78-79	0.5171233688599912
2307	80-81	0.5100805045672487
2307	82-83	0.48875722253617226
2307	84-85	0.49589726041187276
2307	86-87	0.5077156265484817
2307	88-89	0.518106605344407
2307	90-91	0.5428638428570096
2307	92-93	0.508288589079747
2307	94-95	0.5410978668643054
2307	96-97	0.5125488421397506
2307	98-99	0.5792126567765408
2307	100-101	0.685843072022557
2201	1	0.008261827229929963
2201	2	0.06461215213616356
2201	3	0.14350649070566845
2201	4	0.13600917637182164
2201	5	0.14029790232779504
2201	6	0.15583533049669285
2201	7	0.13820190370876162
2201	8	0.11502814903188607
2201	9	0.13260167918422638
2201	10-11	0.14546419833467183
2201	12-13	0.1277615508866532
2201	14-15	0.29245691951349073
2201	16-17	0.25387310304844135
2201	18-19	0.3057453410993958
2201	20-21	0.30092181675863827
2201	22-23	0.3101160569564385
2201	24-25	0.29879147772386716
2201	26-27	0.3135137725076689
2201	28-29	0.3328107336356325
2201	30-31	0.31739017080425924
2201	32-33	0.2734194899953337
2201	34-35	0.2472659897394891
2201	36-37	0.29074866282981304
2201	38-39	0.2976910532290944
2201	40-41	0.3045123029263124
2201	42-43	0.3141620073121345
2201	44-45	0.27683814550624675
2201	46-47	0.26933628201982174
2201	48-49	0.2490272862507581
2201	50-51	0.2929396914667777
2201	52-53	0.28467678404833663
2201	54-55	0.29828878294620154
2201	56-57	0.2791216605692597
2201	58-59	0.2615325439926508
2201	60-61	0.31124633245385525
2201	62-63	0.3032473469065735
2201	64-65	0.35102535177735206
2201	66-67	0.3393267426833688
2201	68-69	0.3103753983077553
2201	70-71	0.20958341643694212
2201	72-73	0.23001270552988018
2201	74-75	0.2730694176611479
2201	76-77	0.08387489710654705
2201	78-79	0.32331922378123323
2201	80-81	0.3765730517524304
2201	82-83	0.3612727973087786
2201	84-85	0.45072809399080427
2201	86-87	0.412029008398342
2201	88-89	0.3812940541732033
2201	90-91	0.3425584463418616
2201	92-93	0.4266979839097971
2201	94-95	0.43745985441620405
2201	96-97	0.4265307705416035
2201	98-99	0.40975321107205076
2201	100-101	0.43087883884207656
1101	1	0.05020767340848664
1101	2	0.08101098413718688
1101	3	0.13586297709845496
1101	4	0.14405443705016552
1101	5	0.11589262300215353
1101	6	0.14689356159266964
1101	7	0.15498505407872898
1101	8	0.1331921791357331
1101	9	0.1166679316784851
1101	10-11	0.12446585017582379
1101	12-13	0.13374614909467653
1101	14-15	0.2826652198160531
1101	16-17	0.2909568622094554
1101	18-19	0.3357659779216533
1101	20-21	0.30612035656563563
1101	22-23	0.27763346109230014
1101	24-25	0.3316947486801851
1101	26-27	0.3103532786846799
1101	28-29	0.3247767917590352
1101	30-31	0.3678572093414303
1101	32-33	0.3266098617946085
1101	34-35	0.3382057338168778
1101	36-37	0.3739985763127862
1101	38-39	0.3527288604201715
1101	40-41	0.33037893951087227
1101	42-43	0.32775224196807073
1101	44-45	0.3520673434984687
1101	46-47	0.3220044192297138
1101	48-49	0.3421683401725417
1101	50-51	0.3257834894405818
1101	52-53	0.3263055137237316
1101	54-55	0.28881123630943506
1101	56-57	0.3639684542525927
1101	58-59	0.3763826021084995
1101	60-61	0.3985626380859415
1101	62-63	0.36969021242510536
1101	64-65	0.37661291091633586
1101	66-67	0.3384060904962709
1101	68-69	0.35983786503448556
1101	70-71	0.3663438861244188
1101	72-73	0.4053366506210807
1101	74-75	0.4337897645052635
1101	76-77	0.35200468924294626
1101	78-79	0.5312139500277127
1101	80-81	0.4589369592809689
1101	82-83	0.49637785817310487
1101	84-85	0.5566875993726867
1101	86-87	0.524007617439814
1101	88-89	0.5466560388851036
1101	90-91	0.5638846694967654
1101	92-93	0.5452969727578747
1101	94-95	0.5429071065898441
1101	96-97	0.49314005341751965
1101	98-99	0.5213404063763178
1101	100-101	0.5091154241544302
2304	1	0.024976081604087597
2304	2	0.040069682376817184
2304	3	0.1046183924019175
2304	4	0.10585084501581576
2304	5	0.0898770463573868
2304	6	0.13120758617264983
2304	7	0.10546014359312039
2304	8	0.12347193452318805
2304	9	0.1007583410520354
2304	10-11	0.11987916490274841
2304	12-13	0.12598561819836362
2304	14-15	0.2597346222762056
2304	16-17	0.2898717592433755
2304	18-19	0.2735466922809593
2304	20-21	0.234049099637879
2304	22-23	0.25562824827066066
2304	24-25	0.26848315934914524
2304	26-27	0.28814663278831176
2304	28-29	0.2963637716991272
2304	30-31	0.2651331442137561
2304	32-33	0.3072437223832907
2304	34-35	0.24914991877139414
2304	36-37	0.31338188241717546
2304	38-39	0.2815480763641318
2304	40-41	0.28513451808586154
2304	42-43	0.3278150418942971
2304	44-45	0.3010081953513932
2304	46-47	0.3305038669463727
2304	48-49	0.3002642511011331
2304	50-51	0.3118844692932967
2304	52-53	0.32905320713390296
2304	54-55	0.34581451959610376
2304	56-57	0.3554920166165587
2304	58-59	0.3649989253037731
2304	60-61	0.3309219704599329
2304	62-63	0.3391889464773854
2304	64-65	0.37268320311800096
2304	66-67	0.333336779993644
2304	68-69	0.34081073621151603
2304	70-71	0.37162971217140495
2304	72-73	0.3966514387631648
2304	74-75	0.42327906325157016
2304	76-77	0.25148436887860015
2304	78-79	0.4333707956239792
2304	80-81	0.41333510540396645
2304	82-83	0.3965510554295335
2304	84-85	0.4882650029794888
2304	86-87	0.42295522259615126
2304	88-89	0.46879973720435686
2304	90-91	0.494735679010752
2304	92-93	0.42083608825285523
2304	94-95	0.5035532934061919
2304	96-97	0.42215986068812583
2304	98-99	0.4950139901055053
2304	100-101	0.46707379206728206
2202	1	0.05210295855998481
2202	2	0.06048670461616723
2202	3	0.12754950175650492
2202	4	0.12257783039878234
2202	5	0.10818175527015939
2202	6	0.08966844389323114
2202	7	0.1001248189335513
2202	8	0.1115512627044879
2202	9	0.11357330970938762
2202	10-11	0.11563240224824511
2202	12-13	0.11965338673803672
2202	14-15	0.24979001472890872
2202	16-17	0.21588427269991683
2202	18-19	0.2558711130824065
2202	20-21	0.26692956082007413
2202	22-23	0.2740917236896152
2202	24-25	0.26344408400222363
2202	26-27	0.2676975151350405
2202	28-29	0.27897633600766625
2202	30-31	0.268002527859025
2202	32-33	0.26776800827076386
2202	34-35	0.2964122008250598
2202	36-37	0.3360659792677225
2202	38-39	0.29303085587823574
2202	40-41	0.28732796694996665
2202	42-43	0.3223528256168606
2202	44-45	0.33107458905107734
2202	46-47	0.3375645242389993
2202	48-49	0.28941156088559694
2202	50-51	0.3148321037629529
2202	52-53	0.29993911446381105
2202	54-55	0.3288444732893012
2202	56-57	0.34132333671017534
2202	58-59	0.3535984077957721
2202	60-61	0.3374897232528937
2202	62-63	0.29953320003938444
2202	64-65	0.34122625251490035
2202	66-67	0.32717210030229893
2202	68-69	0.32036830584782905
2202	70-71	0.3613130183483051
2202	72-73	0.363896665248312
2202	74-75	0.3524664479002979
2202	76-77	0.3836353016005347
2202	78-79	0.4000302584546347
2202	80-81	0.347781179088841
2202	82-83	0.419832436906745
2202	84-85	0.4520570728553466
2202	86-87	0.41480673043691496
2202	88-89	0.3611740575832201
2202	90-91	0.391110840247741
2202	92-93	0.4206974551372369
2202	94-95	0.362559403633945
2202	96-97	0.38887269667227997
2202	98-99	0.43182449151037616
2202	100-101	0.49124408836666333
1102	1	0.05946575423183731
1102	2	0.06636228218450668
1102	3	0.1113220958961989
1102	4	0.11870675738778402
1102	5	0.09714947347806913
1102	6	0.10490178189220245
1102	7	0.09200822278580745
1102	8	0.061166790577253494
1102	9	0.09289087005447527
1102	10-11	0.10051721700021687
1102	12-13	0.0990828963585102
1102	14-15	0.2153570501895885
1102	16-17	0.22310391926340856
1102	18-19	0.22566740710524869
1102	20-21	0.23345724878212337
1102	22-23	0.2383141688546786
1102	24-25	0.24414105751417026
1102	26-27	0.26377280030595074
1102	28-29	0.24129538284103802
1102	30-31	0.2521438899493731
1102	32-33	0.23663537092568276
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1213	48-49	-0.6360396750437403
1213	50-51	-0.6501788916387738
1213	52-53	-0.6134735355240934
1213	54-55	-0.6836013435290624
1213	56-57	-1.095805389082777
1213	58-59	-0.9793674363798459
1213	60-61	-0.6363869298960552
1213	62-63	-0.6694679879296572
1213	64-65	-0.6341583100782344
1213	66-67	-0.5620470745247346
1213	68-69	-0.6235739478358795
1213	70-71	-0.5425069300070646
1213	72-73	-0.44671345493391357
1213	74-75	-0.4291170449867323
1213	76-77	-0.24221339940577735
1213	78-79	-0.5928775183773993
1213	80-81	-0.5765966563778377
1213	82-83	-0.601251198808157
1213	84-85	-0.5508989559188251
1213	86-87	-0.5276479449892904
1213	88-89	-0.586422976942039
1213	90-91	-0.5822911212984181
1213	92-93	-0.6917940031758789
1213	94-95	-0.6092923543957127
1213	96-97	-0.6926104521458072
1213	98-99	-0.6010604426195343
1213	100-101	-0.2936541604272733
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	49.0
8	471.0
9	1428.0
10	1697.0
11	1210.0
12	940.0
13	921.0
14	1054.0
15	1454.0
16	2395.0
17	3645.0
18	5373.0
19	7489.0
20	10589.0
21	14406.0
22	19582.0
23	25371.0
24	33016.0
25	42125.0
26	53001.0
27	68430.0
28	88286.0
29	117787.0
30	175107.0
31	199222.0
32	203044.0
33	270101.0
34	389485.0
35	634752.0
36	1462131.0
37	4292057.0
38	4243608.0
39	3624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.822816918661417	13.358419940219665	14.46261576948025	49.35614737163867
2	22.935140184166634	19.9242043305392	36.59466126557465	20.545994219719514
3	24.67585271864758	23.194110465701737	26.704439197765684	25.425597617885
4	26.847191649959846	27.899173803382322	18.402983944400088	26.85065060225774
5	29.927073485597745	29.67916309797794	21.112863555702173	19.28089986072214
6	22.761400411125006	33.02344330609417	22.04899492898412	22.1661613537967
7	19.347693830506458	17.36924766838976	40.38274419315828	22.900314307945496
8	21.247930410557572	22.132935224345637	26.952259166946764	29.666875198150034
9	23.40079710020776	20.390214785177648	29.947295312728812	26.261692801885783
10-11	25.504120523832054	29.168888926782415	20.79542089219351	24.531569657192023
12-13	22.8754621441809	23.579285466989887	27.419918983947106	26.125333404882106
14-15	23.424370787807636	25.366134719926777	25.882563489960038	25.326931002305553
16-17	25.148831026678703	24.62694859866408	24.93431263114441	25.289907743512806
18-19	24.297295042072207	24.888934505473014	25.806015071925927	25.007755380528852
20-21	24.847374728962958	24.86842732651407	26.007425384502863	24.276772560020106
22-23	24.076686964601198	26.44976655179796	25.082943148515696	24.39060333508515
24-25	24.04693380848583	24.9896354021367	25.713868244100546	25.249562545276927
26-27	23.980520535582635	24.88401044735344	25.132671908984843	26.00279710807908
28-29	24.917309367057836	25.66028858319719	24.77310989837263	24.64929215137235
30-31	24.251514564485692	24.674614119649757	25.709141295398485	25.364730020466062
32-33	24.16497339398946	25.72283141681963	24.76734015040393	25.344855038786985
34-35	24.09256354591213	25.70069858707797	25.367757367084597	24.838980499925302
36-37	24.091288944405783	24.86945846056049	25.63781621260003	25.4014363824337
38-39	24.18581885464722	24.864436712453188	25.610167690227314	25.339576742672282
40-41	24.41049870727444	24.71522356381481	26.394824763598685	24.479452965312063
42-43	24.112865611405603	25.57527282049395	25.90948891886925	24.402372649231197
44-45	24.22009997634397	24.710092335336988	25.844603370525444	25.225204317793597
46-47	25.11982029169134	24.66059051484783	24.844674480578664	25.374914712882163
48-49	24.134971797182203	25.610276321535363	25.64283489999937	24.61191698128306
50-51	24.708342727377154	24.892770475910005	26.007513726082376	24.391373070630465
52-53	24.442599781133797	24.75779460662409	24.812444441928587	25.987161170313527
54-55	24.97871838867482	24.804721276900725	25.594840899915067	24.621719434509387
56-57	24.00583343691362	24.781484680617023	25.89073453640658	25.321947346062778
58-59	24.36886731043824	24.671254546815142	25.682599889849158	25.27727825289746
60-61	25.036861056653027	24.471334020675837	25.80251321177181	24.689291710899326
62-63	24.162072557396613	24.590662458251515	25.91580619693471	25.331458787417162
64-65	24.979758400930653	24.67001382751457	25.63179904680543	24.71842872474934
66-67	24.198214262404324	26.24281280283645	24.755824330843467	24.80314860391576
68-69	24.207655007471253	26.364719423755417	24.90479561960196	24.52282994917137
70-71	24.36478342073243	26.323947514601294	24.70346907935411	24.607799985312163
72-73	24.235507659714322	26.324563669920725	25.00198256948365	24.437946100881305
74-75	24.531455709712624	26.149186357212066	24.934533779542694	24.384824153532616
76-77	24.786455849758557	26.053598856215427	24.686588104093968	24.473357189932052
78-79	24.38887615190093	25.998331649909005	24.84726222990294	24.765529968287122
80-81	24.56740587214207	26.0196499356179	24.750830000951204	24.662114191288822
82-83	24.541377372517115	25.949191718015584	24.684150978117597	24.825279931349705
84-85	24.457908383618356	25.801665262318963	24.783592315632436	24.95683403843024
86-87	24.500039640108227	26.083255864735825	24.838350386392055	24.578354108763893
88-89	24.632646510945474	25.94704992441817	24.808871447200264	24.611432117436095
90-91	24.742193931842266	25.642584670648056	24.825628696386758	24.789592701122917
92-93	24.64453649142495	25.86627549079563	25.065912331487443	24.423275686291973
94-95	24.59245030232103	25.76414280307686	24.90178213312082	24.741624761481283
96-97	24.374317714483734	25.88811471157082	24.902318211014645	24.8352493629308
98-99	24.629245358993142	25.632522390665702	25.028203629904855	24.710028620436297
100-101	24.725344173397932	25.74808568068952	24.897049018696688	24.629521127215863
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	2.0
4	2.0
5	8.0
6	11.0
7	12.0
8	20.5
9	29.5
10	38.5
11	46.0
12	67.0
13	100.0
14	139.0
15	203.5
16	290.5
17	453.0
18	684.5
19	961.0
20	1420.5
21	2096.5
22	3042.5
23	4548.0
24	6561.0
25	9474.0
26	13586.0
27	18869.5
28	26002.5
29	34981.0
30	45346.5
31	57746.5
32	73972.5
33	95397.0
34	122513.0
35	156263.5
36	194877.0
37	241537.0
38	289593.0
39	334714.0
40	383436.5
41	430162.5
42	469029.0
43	500758.0
44	524051.0
45	523463.5
46	507843.5
47	504809.0
48	481743.5
49	449410.0
50	441963.5
51	415951.0
52	383973.0
53	366528.5
54	353949.5
55	352041.5
56	355613.0
57	354791.0
58	332489.5
59	289233.5
60	261904.5
61	241940.5
62	214906.0
63	193797.0
64	166542.5
65	148231.0
66	146717.0
67	144644.5
68	131778.0
69	110952.5
70	91976.5
71	78543.5
72	63813.5
73	50707.5
74	47048.0
75	39786.5
76	26058.5
77	16049.5
78	11877.0
79	10930.5
80	8847.0
81	5636.0
82	2528.5
83	699.0
84	384.0
85	223.0
86	146.5
87	93.5
88	70.5
89	58.5
90	37.0
91	21.0
92	19.0
93	16.0
94	9.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11531574807909453
2	4.6873035171262755E-4
3	0.002505282914326113
4	0.0013172939194682465
5	2.1012050249186753E-4
6	0.0
7	0.0
8	1.6971271355112376E-4
9	0.0
10-11	4.040778894074376E-6
12-13	6.465246230519001E-5
14-15	2.0607972359779314E-4
16-17	7.111770853570901E-4
18-19	0.0014910474119134444
20-21	6.626877386281976E-4
22-23	1.2122336682223127E-4
24-25	0.0
26-27	4.040778894074376E-6
28-29	1.6163115576297503E-4
30-31	0.02332741655549137
32-33	0.03685594429285238
34-35	0.057415427305902794
36-37	0.04885705760825327
38-39	0.041882673237080906
40-41	0.05623147908993901
42-43	0.067476966752148
44-45	0.056785065798427195
46-47	0.025363969118104852
48-49	0.018623949922788798
50-51	0.027679335424409472
52-53	0.06343618785807362
54-55	0.06569902403875527
56-57	0.06661628084771015
58-59	0.05064308187943414
60-61	0.07142076695276459
62-63	0.06331900527014547
64-65	0.04653360974416051
66-67	0.01426799027497662
68-69	0.04943892976899998
70-71	0.07984579094690966
72-73	0.07957505876100668
74-75	0.06873768976709921
76-77	0.024171939344352914
78-79	0.01413060379257809
80-81	5.737906029585614E-4
82-83	0.003365968818763955
84-85	0.0078108256022457676
86-87	1.6971271355112376E-4
88-89	0.0014344765073964034
90-91	9.132160300608089E-4
92-93	0.0017698611556045767
94-95	6.869324119926438E-4
96-97	0.0
98-99	1.2122336682223127E-5
100-101	1.6163115576297503E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.2373852E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.293133311352065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55341937565817	39.26664745475477
2	14.302093195667151	14.671983458334728
3	3.8136393825196646	5.868405397470106
4	1.7527030026531207	3.5960651508117434
5	0.877810117216347	2.2512815682215837
6	0.5691441194620074	1.7515911115762097
7	0.41164911257593645	1.4780340966200098
8	0.25570756496927727	1.0492833774952286
9	0.22220517524380695	1.0257839708627643
>10	1.0718203379176243	9.71707244128724
>50	0.0824253274000233	2.961777763329005
>100	0.07386373268321784	7.537533179112274
>500	0.008986686498847044	3.1459389207978457
>1k	0.004389155778670842	3.7555658968392858
>5k	9.645773297723564E-5	0.29019914215340575
>10k+	4.7256023094128426E-5	1.6328370703335586
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT	176977	1.4302498526732017	TruSeq Adapter, Index 27 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA	14880	0.12025357988765342	TruSeq Adapter, Index 27 (97% over 44bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.1272279642588258	4.848934672889251E-5	0.0
2	0.12742192164574137	4.848934672889251E-5	8.081557788148751E-6
3	0.1276805314949621	4.848934672889251E-5	8.081557788148751E-6
4	0.12870688933405702	4.848934672889251E-5	8.081557788148751E-6
5	0.12886043893203183	6.465246230519001E-5	8.081557788148751E-6
6	0.129466555766143	7.273402009333876E-5	8.081557788148751E-6
7	0.12962010536411783	8.081557788148751E-5	8.081557788148751E-6
8	0.12999185702237265	8.081557788148751E-5	8.081557788148751E-6
9	0.13030703777611047	8.081557788148751E-5	8.081557788148751E-6
10-11	0.1310586226504083	1.0910103014000815E-4	8.081557788148751E-6
12-13	0.13183445219807055	1.333457035044544E-4	1.6163115576297503E-5
14-15	0.13280019835375434	1.5354959797482626E-4	1.6163115576297503E-5
16-17	0.1337538221727559	2.101205024918675E-4	1.6163115576297503E-5
18-19	0.13453369249931224	2.303243969622394E-4	1.6163115576297503E-5
20-21	0.1358024970720516	2.8285452258520625E-4	2.0203894470371878E-5
22-23	0.13642477702173905	3.2326231152595005E-4	2.4244673364446254E-5
24-25	0.13710362787594355	3.636701004666938E-4	3.2326231152595005E-5
26-27	0.137616806795491	3.636701004666938E-4	4.0407788940743756E-5
28-29	0.1381703935039792	3.9195555272521443E-4	4.0407788940743756E-5
30-31	0.13876438800140814	4.0003711051336317E-4	4.0407788940743756E-5
32-33	0.1393826271722015	4.364041205600326E-4	4.0407788940743756E-5
34-35	0.13992409154400748	4.808526883948507E-4	4.0407788940743756E-5
36-37	0.14046151513691935	5.172196984415201E-4	4.0407788940743756E-5
38-39	0.14094236782531422	5.333828140178176E-4	4.0407788940743756E-5
40-41	0.14158081089057797	5.535867084881895E-4	4.444856783481813E-5
42-43	0.1424091705638632	5.939944974289331E-4	5.657090451704126E-5
44-45	0.14299508350350398	6.465246230519001E-4	5.657090451704126E-5
46-47	0.14377495383006036	6.465246230519001E-4	5.657090451704126E-5
48-49	0.14456694649329893	6.546061808400488E-4	6.465246230519001E-5
50-51	0.14558522277460567	6.869324119926438E-4	6.465246230519001E-5
52-53	0.1465913767192302	7.030955275689414E-4	6.465246230519001E-5
54-55	0.1478359366186051	7.192586431452389E-4	6.465246230519001E-5
56-57	0.1490845372968741	7.31380979827462E-4	7.677479898741314E-5
58-59	0.15050689146758828	7.394625376156106E-4	8.081557788148751E-5
60-61	0.15197773498503134	7.515848742978338E-4	8.081557788148751E-5
62-63	0.15440624310037004	7.758295476622801E-4	8.081557788148751E-5
64-65	0.15668928317552205	8.000742210267263E-4	8.081557788148751E-5
66-67	0.16246759699404842	8.041149999208008E-4	8.081557788148751E-5
68-69	0.1648516565415523	8.081557788148751E-4	8.889713566963626E-5
70-71	0.16712661505891618	8.404820099674701E-4	8.889713566963626E-5
72-73	0.17022185169177714	8.445227888615446E-4	8.889713566963626E-5
74-75	0.1732524358623329	8.687674622259908E-4	8.889713566963626E-5
76-77	0.1767436688268132	9.212975878489576E-4	9.697869345778502E-5
78-79	0.1809056710877098	9.212975878489576E-4	1.0506025124593377E-4
80-81	0.18553640370031904	9.212975878489576E-4	1.1314180903408252E-4
82-83	0.19104398533294242	9.212975878489576E-4	1.1314180903408252E-4
84-85	0.1982325309855007	9.334199245311808E-4	1.1314180903408252E-4
86-87	0.2067343297786332	9.495830401074783E-4	1.2122336682223127E-4
88-89	0.21695345960174728	9.980723868363707E-4	1.2122336682223127E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGAA	27865	0.0	63.589386	2
GAGCACA	28415	0.0	62.48945	9
TCGGAAG	28775	0.0	61.59464	3
AGAGCAC	29305	0.0	60.54327	8
GATCGGA	30915	0.0	57.433598	1
CGGAAGA	31535	0.0	56.638786	4
AAGAGCA	37630	0.0	47.199562	7
GAAGAGC	40910	0.0	43.624187	6
CGTATGC	22815	0.0	41.790394	46-47
TATGCCG	22905	0.0	41.54125	48-49
CACACGT	23410	0.0	41.17133	12-13
CTCGTAT	23310	0.0	40.80188	44-45
GGAAGAG	44110	0.0	40.685623	5
CACGTCT	25115	0.0	38.358173	14-15
CGTCTGA	25405	0.0	37.995148	16-17
TCGTATG	23115	0.0	37.991276	44-45
ACGTCTG	23335	0.0	37.937057	14-15
TATCTCG	23455	0.0	37.285915	40-41
ACACGTC	23770	0.0	37.28199	12-13
GCACACG	23860	0.0	37.17996	10-11
>>END_MODULE