FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n01 mx48-1.3410000000cc1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n01 mx48-1.3410000000cc1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12373852
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT1769771.4302498526732017TruSeq Adapter, Index 27 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA148800.12025357988765342TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA278650.063.5893862
GAGCACA284150.062.489459
TCGGAAG287750.061.594643
AGAGCAC293050.060.543278
GATCGGA309150.057.4335981
CGGAAGA315350.056.6387864
AAGAGCA376300.047.1995627
GAAGAGC409100.043.6241876
CGTATGC228150.041.79039446-47
TATGCCG229050.041.5412548-49
CACACGT234100.041.1713312-13
CTCGTAT233100.040.8018844-45
GGAAGAG441100.040.6856235
CACGTCT251150.038.35817314-15
CGTCTGA254050.037.99514816-17
TCGTATG231150.037.99127644-45
ACGTCTG233350.037.93705714-15
TATCTCG234550.037.28591540-41
ACACGTC237700.037.2819912-13
GCACACG238600.037.1799610-11
Produced by FastQC (version 0.11.2)