##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l06n01 mx24-1.3410000000ca51.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17765481 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.80919655369871 33.0 33.0 33.0 33.0 33.0 2 32.81057377506413 33.0 33.0 33.0 33.0 33.0 3 32.73375322627065 33.0 33.0 33.0 33.0 33.0 4 36.727538984168234 37.0 37.0 37.0 37.0 37.0 5 36.74208016095933 37.0 37.0 37.0 37.0 37.0 6 36.68831471548673 37.0 37.0 37.0 37.0 37.0 7 36.70900500808281 37.0 37.0 37.0 37.0 37.0 8 36.706734819057246 37.0 37.0 37.0 37.0 37.0 9 36.7063839138383 37.0 37.0 37.0 37.0 37.0 10-11 36.688827254381685 37.0 37.0 37.0 37.0 37.0 12-13 36.68557786304801 37.0 37.0 37.0 37.0 37.0 14-15 39.107806256413774 40.0 40.0 40.0 37.0 40.0 16-17 39.09220017741146 40.0 40.0 40.0 37.0 40.0 18-19 39.0591014394713 40.0 40.0 40.0 37.0 40.0 20-21 39.049491764394105 40.0 40.0 40.0 37.0 40.0 22-23 39.01543377857318 40.0 40.0 40.0 37.0 40.0 24-25 38.98393471586837 40.0 40.0 40.0 37.0 40.0 26-27 38.923966060924556 40.0 40.0 40.0 37.0 40.0 28-29 38.87670494257938 40.0 40.0 40.0 37.0 40.0 30-31 38.8178206376737 40.0 40.0 40.0 37.0 40.0 32-33 38.740010276107924 40.0 38.5 40.0 37.0 40.0 34-35 38.655214598467666 40.0 37.0 40.0 37.0 40.0 36-37 38.56997395679858 40.0 37.0 40.0 37.0 40.0 38-39 38.46771427128823 40.0 37.0 40.0 37.0 40.0 40-41 38.349232171085035 40.0 37.0 40.0 37.0 40.0 42-43 38.22385113017768 40.0 37.0 40.0 37.0 40.0 44-45 38.09874126121325 40.0 37.0 40.0 37.0 40.0 46-47 37.965213691652934 40.0 37.0 40.0 35.0 40.0 48-49 37.820942281270064 40.0 37.0 40.0 33.0 40.0 50-51 37.669927963110034 40.0 37.0 40.0 33.0 40.0 52-53 37.486023823390994 38.5 37.0 40.0 33.0 40.0 54-55 37.30145584012051 37.0 37.0 40.0 33.0 40.0 56-57 37.12342891250735 37.0 37.0 40.0 33.0 40.0 58-59 36.951567959235106 37.0 37.0 40.0 33.0 40.0 60-61 36.764980300842964 37.0 37.0 40.0 33.0 40.0 62-63 36.56228787163151 37.0 37.0 40.0 33.0 40.0 64-65 36.37764184375306 37.0 37.0 40.0 33.0 40.0 66-67 36.143952955734775 37.0 37.0 37.0 33.0 40.0 68-69 35.93904535993143 37.0 37.0 37.0 33.0 40.0 70-71 35.737877544660904 37.0 37.0 37.0 33.0 40.0 72-73 35.50647455028097 37.0 37.0 37.0 33.0 38.5 74-75 35.291293858016004 37.0 35.0 37.0 33.0 37.0 76-77 32.62196880568559 33.0 33.0 35.0 27.0 37.0 78-79 34.7990082002283 37.0 33.0 37.0 33.0 37.0 80-81 35.03557224259788 37.0 33.0 37.0 33.0 37.0 82-83 35.0020582611864 37.0 33.0 37.0 33.0 37.0 84-85 34.916147499749655 37.0 33.0 37.0 33.0 37.0 86-87 34.856500986379146 37.0 33.0 37.0 33.0 37.0 88-89 34.78424217728752 37.0 33.0 37.0 33.0 37.0 90-91 34.704855162660664 37.0 33.0 37.0 33.0 37.0 92-93 34.619224297951746 37.0 33.0 37.0 33.0 37.0 94-95 34.54916697724086 37.0 33.0 37.0 33.0 37.0 96-97 34.47502865247499 37.0 33.0 37.0 33.0 37.0 98-99 34.42565484154355 37.0 33.0 37.0 27.0 37.0 100-101 32.61281785728177 35.0 30.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.04751361157033074 2306 2 0.06452003182077704 2306 3 0.13941179407763826 2306 4 0.1321145144997331 2306 5 0.12970875539713234 2306 6 0.1579672548178621 2306 7 0.13320640484769086 2306 8 0.16616506186236535 2306 9 0.13976223725259018 2306 10-11 0.15922845984839995 2306 12-13 0.15378286990100776 2306 14-15 0.3259104613841899 2306 16-17 0.3445682719392664 2306 18-19 0.37219542998875 2306 20-21 0.3434019448574688 2306 22-23 0.366081597338642 2306 24-25 0.35172115650876634 2306 26-27 0.36518211232148445 2306 28-29 0.36917688386502334 2306 30-31 0.38398221130989896 2306 32-33 0.35903621390903595 2306 34-35 0.3822193960257181 2306 36-37 0.3483333230063863 2306 38-39 0.4053209436981575 2306 40-41 0.3697006131999885 2306 42-43 0.3562458521831289 2306 44-45 0.38916361127282073 2306 46-47 0.39485970498620304 2306 48-49 0.37420213157663795 2306 50-51 0.3857267441506522 2306 52-53 0.40549733258495735 2306 54-55 0.398416955376355 2306 56-57 0.322042169913793 2306 58-59 0.4010457576448516 2306 60-61 0.3543220261805544 2306 62-63 0.37814443883056015 2306 64-65 0.3820121229054152 2306 66-67 0.4261448780719377 2306 68-69 0.42704655996961094 2306 70-71 0.39072638118384617 2306 72-73 0.35864134591726327 2306 74-75 0.41261972259153623 2306 76-77 0.07713067663595297 2306 78-79 0.4429869322492763 2306 80-81 0.4528542420056496 2306 82-83 0.49769642700324823 2306 84-85 0.5035459648360785 2306 86-87 0.4210960507098491 2306 88-89 0.4199740769091136 2306 90-91 0.4655170100008661 2306 92-93 0.44889073345967745 2306 94-95 0.4811234918179039 2306 96-97 0.37229191586721555 2306 98-99 0.4092154668258914 2306 100-101 0.6582105635108135 2307 1 0.021471392542139256 2307 2 0.05036570569971843 2307 3 0.13061613353589507 2307 4 0.13743946554265563 2307 5 0.11450793735265563 2307 6 0.14780683447972365 2307 7 0.13656846941859158 2307 8 0.12442113375142583 2307 9 0.14407194358730635 2307 10-11 0.13160361573570611 2307 12-13 0.15549737247461337 2307 14-15 0.3282664012276868 2307 16-17 0.34324503434326914 2307 18-19 0.3725741927684254 2307 20-21 0.36671810516621406 2307 22-23 0.3308024026013996 2307 24-25 0.34152510076675213 2307 26-27 0.35480635611341427 2307 28-29 0.3982681529981562 2307 30-31 0.3683538346556361 2307 32-33 0.3796493990266896 2307 34-35 0.4011082796616563 2307 36-37 0.3870580158565673 2307 38-39 0.3913025313718137 2307 40-41 0.3744922254472556 2307 42-43 0.3654131633270623 2307 44-45 0.37411725746971314 2307 46-47 0.4038474315784981 2307 48-49 0.38124573741320233 2307 50-51 0.3980047842892134 2307 52-53 0.44706764573677304 2307 54-55 0.4406177436059906 2307 56-57 0.3874621767406552 2307 58-59 0.38442317060941633 2307 60-61 0.3379458897566394 2307 62-63 0.40075677507381613 2307 64-65 0.42243858430244074 2307 66-67 0.38072648775442275 2307 68-69 0.40257417404195905 2307 70-71 0.40469696225363094 2307 72-73 0.4179868121396737 2307 74-75 0.4238008977125318 2307 76-77 0.22087790560865272 2307 78-79 0.4802426168434337 2307 80-81 0.4645782360657691 2307 82-83 0.524524988743039 2307 84-85 0.5284586640992757 2307 86-87 0.5459813875467532 2307 88-89 0.5426499317694535 2307 90-91 0.5162482838424438 2307 92-93 0.49723725444533073 2307 94-95 0.5518974407115564 2307 96-97 0.5570858311683651 2307 98-99 0.540290012605098 2307 100-101 0.6443628848423373 2201 1 0.019460044759128436 2201 2 0.0461683726887685 2201 3 0.12161594014104082 2201 4 0.1284931127232838 2201 5 0.12345307090618007 2201 6 0.13407361639112025 2201 7 0.11218034196811288 2201 8 0.10210004766798164 2201 9 0.10887972925414147 2201 10-11 0.12827513707810567 2201 12-13 0.10097524460772433 2201 14-15 0.23279417594869045 2201 16-17 0.22272034360712212 2201 18-19 0.2595874719167597 2201 20-21 0.25205743563427774 2201 22-23 0.25239767074775443 2201 24-25 0.2609229900935688 2201 26-27 0.2486862615273182 2201 28-29 0.3043932865275707 2201 30-31 0.2822924094888535 2201 32-33 0.28777348446967466 2201 34-35 0.2447857128935098 2201 36-37 0.2358032621405215 2201 38-39 0.25757764493316415 2201 40-41 0.26908068964242915 2201 42-43 0.27284352094566344 2201 44-45 0.28867903558640506 2201 46-47 0.2909946164262607 2201 48-49 0.25165528502252243 2201 50-51 0.2546598248771659 2201 52-53 0.30365279552651003 2201 54-55 0.3397840757495061 2201 56-57 0.32441766303226416 2201 58-59 0.30804766910453196 2201 60-61 0.34479238435871906 2201 62-63 0.35383233700812866 2201 64-65 0.35317021097357326 2201 66-67 0.3424516214904969 2201 68-69 0.3002519341334988 2201 70-71 0.23382834383165374 2201 72-73 0.2541144995832809 2201 74-75 0.2602811458641341 2201 76-77 0.023454703427340462 2201 78-79 0.2832533561990047 2201 80-81 0.29204207183133946 2201 82-83 0.3089636675066103 2201 84-85 0.4158759852172267 2201 86-87 0.36103361362826547 2201 88-89 0.33380634859511815 2201 90-91 0.29775924009990007 2201 92-93 0.33508806762503696 2201 94-95 0.4046841537447676 2201 96-97 0.31639175203009273 2201 98-99 0.3355812767891848 2201 100-101 0.3578049601568054 1101 1 0.04665435699838838 1101 2 0.06029468529736448 1101 3 0.13278857912436592 1101 4 0.12697036084093583 1101 5 0.12685212910164267 1101 6 0.13770930087820688 1101 7 0.130487360174655 1101 8 0.14584426631436997 1101 9 0.13721612639012193 1101 10-11 0.14637608307963745 1101 12-13 0.13603769581771985 1101 14-15 0.2728090332929938 1101 16-17 0.28094346398518155 1101 18-19 0.3339970813448758 1101 20-21 0.3288701575501207 1101 22-23 0.311289455559681 1101 24-25 0.33518755155009927 1101 26-27 0.32561205035157315 1101 28-29 0.3313011542048727 1101 30-31 0.3775889268266184 1101 32-33 0.3399089549102712 1101 34-35 0.33757201056081954 1101 36-37 0.3781993236851804 1101 38-39 0.3566919541505982 1101 40-41 0.3260351858777284 1101 42-43 0.35710250040559544 1101 44-45 0.38395771571700976 1101 46-47 0.3538324111598783 1101 48-49 0.37094897358907986 1101 50-51 0.365562891197996 1101 52-53 0.37953294628238865 1101 54-55 0.3496154826644684 1101 56-57 0.39272875231903015 1101 58-59 0.4167062691565775 1101 60-61 0.42046860337444514 1101 62-63 0.34749706466544694 1101 64-65 0.40917774037348664 1101 66-67 0.3763050458145685 1101 68-69 0.40082100399659737 1101 70-71 0.3803780403892816 1101 72-73 0.4074789745818066 1101 74-75 0.38768375471305916 1101 76-77 0.3457459241638219 1101 78-79 0.5103432053591916 1101 80-81 0.4389355858031294 1101 82-83 0.46082364752894023 1101 84-85 0.5160086940384545 1101 86-87 0.4742299152407483 1101 88-89 0.49939359142456397 1101 90-91 0.4700910457289069 1101 92-93 0.472519719644211 1101 94-95 0.516928787893157 1101 96-97 0.45980168042944314 1101 98-99 0.46653185303590305 1101 100-101 0.4619628760994914 2304 1 0.04652906084383801 2304 2 0.04902356394559604 2304 3 0.12486367871039761 2304 4 0.11623947745422925 2304 5 0.10859338766083226 2304 6 0.14250798087508088 2304 7 0.11777842476889333 2304 8 0.13776998684862463 2304 9 0.13387934334448204 2304 10-11 0.11670139745907449 2304 12-13 0.13184076280722223 2304 14-15 0.25652102136870525 2304 16-17 0.2721660615534631 2304 18-19 0.26834140819535435 2304 20-21 0.2698315858611906 2304 22-23 0.2730298679630252 2304 24-25 0.27998580846904986 2304 26-27 0.32203997089102643 2304 28-29 0.2996137567199142 2304 30-31 0.2666661938734052 2304 32-33 0.28913586136448544 2304 34-35 0.22342835282435658 2304 36-37 0.2829950746810397 2304 38-39 0.2790900249152557 2304 40-41 0.30683330610996506 2304 42-43 0.30508897492978804 2304 44-45 0.30546016306008283 2304 46-47 0.29830424718789317 2304 48-49 0.3288086223693085 2304 50-51 0.34902396152006077 2304 52-53 0.332083455277683 2304 54-55 0.33163759987657926 2304 56-57 0.3486492397047982 2304 58-59 0.3586655104802219 2304 60-61 0.37151686029339004 2304 62-63 0.33875025402211634 2304 64-65 0.3817578973679403 2304 66-67 0.31115072000551436 2304 68-69 0.33053897374325913 2304 70-71 0.3305057773780149 2304 72-73 0.4102708007163045 2304 74-75 0.41082481540195914 2304 76-77 0.22970362288027246 2304 78-79 0.4565154891989138 2304 80-81 0.4774571562683789 2304 82-83 0.46165060214894993 2304 84-85 0.4623162000917205 2304 86-87 0.48974776262026865 2304 88-89 0.4717932894120125 2304 90-91 0.469059061037818 2304 92-93 0.47176924449515667 2304 94-95 0.46905963613185264 2304 96-97 0.40314764101159284 2304 98-99 0.4427864418041594 2304 100-101 0.44334191486644414 2202 1 0.05086404413988532 2202 2 0.07752718058213048 2202 3 0.1419426232110368 2202 4 0.11663691300238099 2202 5 0.11204746852319403 2202 6 0.11653782607259444 2202 7 0.12519149381304828 2202 8 0.13087411328786658 2202 9 0.12119144511431301 2202 10-11 0.1216783876591947 2202 12-13 0.12722884175569504 2202 14-15 0.24032573917229882 2202 16-17 0.2440741338839274 2202 18-19 0.2785836850984822 2202 20-21 0.2944636078516112 2202 22-23 0.276341868703156 2202 24-25 0.27281893548609304 2202 26-27 0.2997474819390149 2202 28-29 0.3057779949842896 2202 30-31 0.32103479934558266 2202 32-33 0.30299965600628553 2202 34-35 0.3214983945076213 2202 36-37 0.356875319286452 2202 38-39 0.3269250802942949 2202 40-41 0.3202835671412174 2202 42-43 0.37366335776881243 2202 44-45 0.3608510487046672 2202 46-47 0.35525146569244725 2202 48-49 0.34743332850339925 2202 50-51 0.3470969262538759 2202 52-53 0.3873889764944849 2202 54-55 0.36675210683457493 2202 56-57 0.4061917033993794 2202 58-59 0.38406445012235224 2202 60-61 0.41796704243066074 2202 62-63 0.392060119232589 2202 64-65 0.4065498996178434 2202 66-67 0.411866909959528 2202 68-69 0.4110087817527699 2202 70-71 0.4260236553795309 2202 72-73 0.42953667657009476 2202 74-75 0.42541327826332775 2202 76-77 0.48961460180138516 2202 78-79 0.5153797606101236 2202 80-81 0.43952901449986115 2202 82-83 0.44307755312910757 2202 84-85 0.5066020903280588 2202 86-87 0.49930142699049185 2202 88-89 0.4168646572520984 2202 90-91 0.45667249242672625 2202 92-93 0.4849784815619387 2202 94-95 0.44292989837773433 2202 96-97 0.4915317786662783 2202 98-99 0.5147828938451227 2202 100-101 0.6403636810927864 1102 1 0.0358612553152966 1102 2 0.0520313731830484 1102 3 0.1132662745227293 1102 4 0.10242638882402133 1102 5 0.10159657137786837 1102 6 0.09491843617917795 1102 7 0.10531416903529589 1102 8 0.09121208892292287 1102 9 0.10320557807884256 1102 10-11 0.09722537301424694 1102 12-13 0.10506890890430043 1102 14-15 0.20793351268037696 1102 16-17 0.20810864670621498 1102 18-19 0.21483122391065024 1102 20-21 0.21627101454748754 1102 22-23 0.22825191063814287 1102 24-25 0.24516024933670622 1102 26-27 0.2537717133567696 1102 28-29 0.24067378833057518 1102 30-31 0.24113561270701922 1102 32-33 0.23214719371255654 1102 34-35 0.24254354597788108 1102 36-37 0.22956394361920474 1102 38-39 0.22575124500509247 1102 40-41 0.212748628296751 1102 42-43 0.25044234416311184 1102 44-45 0.24104094368654927 1102 46-47 0.26914664930168897 1102 48-49 0.24708219730557346 1102 50-51 0.25574930717773015 1102 52-53 0.2155949613924264 1102 54-55 0.2610542286755546 1102 56-57 0.28150094559106975 1102 58-59 0.32564274527545933 1102 60-61 0.3146244632758055 1102 62-63 0.26594731428623675 1102 64-65 0.30985491686236344 1102 66-67 0.31817331816776573 1102 68-69 0.24952379764789612 1102 70-71 0.2840426990817875 1102 72-73 0.29128359680977667 1102 74-75 0.2722697048212339 1102 76-77 0.20788738415387087 1102 78-79 0.32537647337659337 1102 80-81 0.32532778892066005 1102 82-83 0.3310516875005973 1102 84-85 0.3634159556150962 1102 86-87 0.3361796708544702 1102 88-89 0.3743377149344056 1102 90-91 0.3535131540748395 1102 92-93 0.3573595628243922 1102 94-95 0.39235940347560216 1102 96-97 0.36927863914723247 1102 98-99 0.42107893111980843 1102 100-101 0.4154577595565456 2305 1 0.027510180882188706 2305 2 0.05239078215835491 2305 3 0.09754607378884828 2305 4 0.08590117314518864 2305 5 0.06909648202732654 2305 6 0.08488656813741358 2305 7 0.08306144021238282 2305 8 0.04879208250497413 2305 9 0.05481160391768469 2305 10-11 0.05193886261451297 2305 12-13 0.07946419685291062 2305 14-15 0.1697862778933299 2305 16-17 0.14713158370729218 2305 18-19 0.1196653463000672 2305 20-21 0.1680303219594137 2305 22-23 0.17554318920331724 2305 24-25 0.18163815318634846 2305 26-27 0.19666288382212116 2305 28-29 0.16855289695045172 2305 30-31 0.1504293783708377 2305 32-33 0.1435610279670101 2305 34-35 0.13682541702985418 2305 36-37 0.19586476520461815 2305 38-39 0.18868558181669215 2305 40-41 0.13338809723152423 2305 42-43 0.21530397696353276 2305 44-45 0.2255361398764535 2305 46-47 0.16976751134426138 2305 48-49 0.2130380875704958 2305 50-51 0.20776443427242697 2305 52-53 0.17221747073409688 2305 54-55 0.22605748447555385 2305 56-57 0.23287102865935339 2305 58-59 0.20772960508837457 2305 60-61 0.16129666203116955 2305 62-63 0.20938435923156362 2305 64-65 0.24090857382240927 2305 66-67 0.15769919360718632 2305 68-69 0.19489372896360635 2305 70-71 0.1557632952515533 2305 72-73 0.18600870866997354 2305 74-75 0.19222241688256503 2305 76-77 0.04698972846365734 2305 78-79 0.18630419289653588 2305 80-81 0.2098170793741403 2305 82-83 0.29085854029543157 2305 84-85 0.32012642984955164 2305 86-87 0.29193684168946277 2305 88-89 0.2775290057506439 2305 90-91 0.2894662787732116 2305 92-93 0.2931942144365962 2305 94-95 0.27535503475964873 2305 96-97 0.3461695785434813 2305 98-99 0.3629944127689271 2305 100-101 0.38333038620067583 2203 1 0.0465759794026539 2203 2 0.023115670184935766 2203 3 0.0906784080885501 2203 4 0.06956425070477223 2203 5 0.0232062631822032 2203 6 0.05854370688201982 2203 7 0.05539888836511864 2203 8 0.0592866650209416 2203 9 0.013349746771780246 2203 10-11 0.054253031450940625 2203 12-13 0.03921962961950243 2203 14-15 0.09331809962352366 2203 16-17 0.10247011424810637 2203 18-19 0.11499077079454878 2203 20-21 0.12749474613184475 2203 22-23 0.14057758185821712 2203 24-25 0.0837768830345027 2203 26-27 0.10471993382143552 2203 28-29 0.12602616252127774 2203 30-31 0.08413578090206641 2203 32-33 0.1460654338570393 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-0.7193181890033884 1213 98-99 -0.6458281240023211 1213 100-101 -0.25356363545383687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 10.0 7 56.0 8 556.0 9 1827.0 10 1961.0 11 1194.0 12 919.0 13 895.0 14 1091.0 15 1950.0 16 3264.0 17 5483.0 18 7898.0 19 11266.0 20 15715.0 21 21258.0 22 28218.0 23 36161.0 24 46734.0 25 59634.0 26 75489.0 27 95395.0 28 120485.0 29 152202.0 30 194854.0 31 248487.0 32 323374.0 33 434503.0 34 618757.0 35 1025508.0 36 2392624.0 37 6415993.0 38 5419844.0 39 1876.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.13120263801625 12.41509578357077 11.385602661369505 55.06809891704347 2 24.688825011936117 19.224947631368515 36.41788991481534 19.66833744188002 3 25.045185598272298 24.54397150181792 23.320677219101334 27.090165680808447 4 27.435894303879305 28.72023448498925 17.184341706561543 26.65952950456991 5 29.612228814230495 30.1146759516547 20.330753213076324 19.942342021038474 6 21.187909294434526 33.56371831418468 21.439008603257072 23.809363788123722 7 19.981294061219057 14.4160521181498 40.342031831279996 25.260621989351144 8 21.802816555746357 19.601347716526515 26.22141383697588 32.37442189075125 9 22.420760800115687 19.000864654325994 30.299900126543157 28.278474419015165 10-11 25.881605788304057 27.48334973217723 20.33281960296035 26.302224876558363 12-13 23.720956858552782 21.902412027648243 26.88631957232987 27.490311541469104 14-15 24.54502186168579 23.56045865789488 25.286582379616064 26.607937100803262 16-17 25.91619318901426 23.75696003424192 23.931890367759546 26.39495640898427 18-19 25.312599492131373 24.194383926709843 24.075423985647465 26.41759259551132 20-21 25.44334474449731 24.23597766236085 24.371283179262825 25.949394413879016 22-23 25.364438182481212 24.277328793288035 24.23352188177042 26.124711142460328 24-25 25.09907556119646 24.430588172647845 24.324553891898564 26.14578237425713 26-27 25.208830540529693 24.42515910489561 24.47139202141501 25.89461833315968 28-29 25.46232505966145 24.022063162544804 24.191687353590464 26.323924424203284 30-31 25.189720409050704 24.05531514277355 24.389364154663927 26.365600293511815 32-33 25.373994211641243 24.313285929533514 24.329032639328467 25.983687219496776 34-35 25.658295201003483 24.146119851459634 23.965896157811077 26.22968878972581 36-37 25.384004641087653 24.286364208863702 23.990270452946152 26.339360697102492 38-39 25.499001453685217 24.383974493075495 24.257309885538128 25.85971416770116 40-41 25.705980236014277 24.055470791897882 24.13928107157984 26.099267900508 42-43 25.086924837235248 24.11685041278948 24.493118222735312 26.30310652723996 44-45 25.510581171359654 24.180118501459617 24.37325608393241 25.93604424324832 46-47 25.721218797736235 23.987347904913747 24.07596898622494 26.215464311125082 48-49 25.42032588356193 23.995298194004082 24.258442591649157 26.32593333078484 50-51 25.572766746758298 24.179046223885845 24.342026863414183 25.90616016594167 52-53 25.889244267552407 23.884294299493682 24.039967877363534 26.186493555590378 54-55 25.372759805352345 23.99298048217617 24.303370983972787 26.330888728498696 56-57 25.54844766295409 24.22428208041096 24.34249224103247 25.88477801560248 58-59 25.75528290867888 24.088436029259793 24.004124619370955 26.152156442690373 60-61 25.352822017077358 24.06413268715283 24.326991930254188 26.25605336551562 62-63 25.55925915318202 24.105489364766402 24.341904552620136 25.993346929431443 64-65 25.73121690714174 24.010217926377702 24.04317316610032 26.215392000380234 66-67 25.41953421873692 24.1040562297764 24.252851704497893 26.223557846988786 68-69 25.69944378903196 24.269578010255934 24.205366140143784 25.825612060568314 70-71 25.799259751550192 24.091215531556607 24.017218853252878 26.092305863640323 72-73 25.457262865353503 24.114817525380285 24.261703509560743 26.16621609970547 74-75 25.68811561392813 24.35552797533935 24.244935489077584 25.71142092165493 76-77 25.86592091643928 24.179911970229146 23.960175356889014 25.993991756442558 78-79 25.56796888181375 24.193670333298073 24.226893908452006 26.011466876436167 80-81 25.873951003383294 24.40150766354733 24.044490040018587 25.680051293050788 82-83 25.929840756422685 24.234136331001658 23.875413496087873 25.960609416487785 84-85 25.46708145988478 24.285384646908987 24.239851628278984 26.00768226492724 86-87 25.77629876500764 24.52297306896857 24.061653589038592 25.639074576985198 88-89 25.87556248194156 24.284770307314226 23.885324468229722 25.9543427425145 90-91 25.65493187542178 24.183703182171605 24.048335518395643 26.113029424010968 92-93 25.77230704661885 24.477334590550253 24.0837159665076 25.666642396323297 94-95 25.917725614213595 24.28227034421314 23.920583310475177 25.87942073109809 96-97 25.588859654292502 24.38429896719374 24.095927940256725 25.930913438257036 98-99 25.824495758755063 24.47275627995293 24.046347862437376 25.656400098854636 100-101 26.074619943391898 24.27424062909423 23.674245531861132 25.976893895652744 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 1.0 3 2.0 4 3.0 5 5.5 6 5.0 7 5.0 8 10.0 9 9.0 10 9.0 11 11.5 12 22.5 13 36.0 14 47.0 15 72.5 16 118.5 17 181.0 18 269.5 19 400.0 20 594.5 21 883.0 22 1341.5 23 2043.0 24 3151.0 25 4746.0 26 7115.5 27 10696.0 28 15682.5 29 22707.5 30 31700.5 31 43431.0 32 59296.5 33 79636.0 34 106270.5 35 141283.0 36 184762.0 37 240629.5 38 307200.5 39 377686.0 40 449039.0 41 518811.5 42 586486.5 43 637892.0 44 665919.5 45 677198.5 46 676029.0 47 664322.5 48 642680.5 49 618537.0 50 593131.5 51 564967.0 52 547004.0 53 542414.0 54 543353.0 55 547746.5 56 555514.5 57 556502.5 58 544081.0 59 512332.0 60 487009.5 61 472443.0 62 448759.0 63 416275.0 64 367516.0 65 332057.0 66 318111.0 67 294764.0 68 258313.0 69 222576.0 70 189304.0 71 157674.0 72 131002.5 73 106818.0 74 82863.5 75 61377.0 76 43723.5 77 31147.0 78 21479.5 79 13966.5 80 8872.0 81 5514.0 82 3143.0 83 1748.0 84 971.5 85 631.0 86 441.0 87 299.0 88 222.0 89 152.0 90 105.0 91 73.0 92 44.5 93 26.5 94 16.0 95 10.0 96 3.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1161353300819719 2 4.2216701028246855E-4 3 0.0023528774706409582 4 0.0014072233676082286 5 1.6886680411298742E-4 6 0.0 7 0.0 8 1.3509344329038994E-4 9 0.0 10-11 5.628893470432914E-6 12-13 7.317561511562788E-5 14-15 2.223412920821001E-4 16-17 6.923538968632484E-4 18-19 0.001446625621901259 20-21 7.317561511562789E-4 22-23 1.0976342267344183E-4 24-25 0.0 26-27 0.0 28-29 1.7731014431863682E-4 30-31 0.022602821730523366 32-33 0.03498638736547578 34-35 0.054988660312659136 36-37 0.04748815976330728 38-39 0.04033665060912226 40-41 0.05574011759096194 42-43 0.06550343331542782 44-45 0.05630863583147566 46-47 0.025161153812835128 48-49 0.018969370995358923 50-51 0.02692018302234541 52-53 0.06204729272458201 54-55 0.06504186405085233 56-57 0.06477730605774197 58-59 0.04892071315153245 60-61 0.07004313589933198 62-63 0.06260736762489008 64-65 0.04538013915863016 66-67 0.01411726482384575 68-69 0.048630825137805166 70-71 0.07885235418055948 72-73 0.0785652806135674 74-75 0.06762834060051626 76-77 0.0237370437648156 78-79 0.014027202528318822 80-81 6.2762162195327E-4 82-83 0.0036672240959870436 84-85 0.008156266638657293 86-87 1.519801237016887E-4 88-89 0.001373450006785631 90-91 9.006229552692664E-4 92-93 0.0017759158899215844 94-95 6.867250033928156E-4 96-97 0.0 98-99 8.443340205649371E-6 100-101 1.4072233676082287E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7765481E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.964227551510945 #Duplication Level Percentage of deduplicated Percentage of total 1 73.88500532698058 36.91607218801855 2 13.149935336396071 13.140527228706958 3 5.03185575647531 7.542383580687381 4 2.5928230645798838 5.181936063979011 5 1.2923751299772006 3.2286262538047192 6 0.8791149666159691 2.6354580141563537 7 0.6015440373699275 2.103897821578396 8 0.41188636517403027 1.6463667259935983 9 0.31388987897084464 1.411493880511396 >10 1.672699090482455 15.015513244604906 >50 0.11441283525269116 3.9051618455979105 >100 0.04955746919935813 4.592377874136829 >500 0.0031806274148174405 1.119400180258702 >1k 0.0016724707177852315 1.404134524537232 >5k 4.764439305732386E-5 0.1566505734281153 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0264220259502121 1.6886680411298742E-5 0.0 2 0.02815572513910544 1.6886680411298742E-5 1.1257786940865828E-5 3 0.03321610036902463 2.814446735216457E-5 1.1257786940865828E-5 4 0.05050806111019454 3.94022542930304E-5 1.1257786940865828E-5 5 0.053767190429575194 3.94022542930304E-5 1.6886680411298742E-5 6 0.062373768545867125 5.628893470432914E-5 2.2515573881731657E-5 7 0.06451837695810206 5.628893470432914E-5 2.2515573881731657E-5 8 0.06938736981002654 5.628893470432914E-5 2.2515573881731657E-5 9 0.0756579571360888 5.628893470432914E-5 2.814446735216457E-5 10-11 0.08785295483978171 7.599006185084433E-5 2.814446735216457E-5 12-13 0.10092887437159737 8.724784879171016E-5 3.095891408738103E-5 14-15 0.11493074687929924 1.0976342267344182E-4 3.94022542930304E-5 16-17 0.12889040268597288 1.4072233676082287E-4 4.2216701028246856E-5 18-19 0.1429176052142917 1.4635123023125577E-4 4.503114776346331E-5 20-21 0.1535899872342325 1.5198012370168867E-4 4.503114776346331E-5 22-23 0.16421733810640984 1.716812508482039E-4 5.628893470432914E-5 24-25 0.17383993149411492 1.8856793125950262E-4 5.628893470432914E-5 26-27 0.1818779913698931 1.9982571820036847E-4 5.91033814395456E-5 28-29 0.18963742101888487 2.1389795187645074E-4 6.191782817476206E-5 30-31 0.19640053652361003 2.364135257581824E-4 7.036116838041143E-5 32-33 0.20214482231018682 2.5611465290469764E-4 8.443340205649371E-5 34-35 0.20784407694900014 2.673724398455634E-4 9.850563573257599E-5 36-37 0.21284253435074457 3.152180343442432E-4 1.1257786940865828E-4 38-39 0.2179788996425146 3.208469278146761E-4 1.1257786940865828E-4 40-41 0.22295765591711253 3.2366137454989256E-4 1.1539231614387475E-4 42-43 0.22750017294775188 3.321047147555419E-4 1.322789965551735E-4 44-45 0.23183160647325002 3.433625016964078E-4 1.4072233676082287E-4 46-47 0.23648107247982758 3.5462028863727363E-4 1.4072233676082287E-4 48-49 0.2408096915585905 3.7713586251900523E-4 1.4635123023125577E-4 50-51 0.24510735172326603 3.883936494598711E-4 1.6042346390733806E-4 52-53 0.24905883493950995 3.9965143640073694E-4 1.6605235737777096E-4 54-55 0.25356757860932666 4.1090922334160275E-4 1.6886680411298742E-4 56-57 0.25764008303518493 4.193525635472521E-4 1.716812508482039E-4 58-59 0.2614705450418145 4.474970308994167E-4 1.8012459105385325E-4 60-61 0.26577101965322525 4.981570721333129E-4 1.8575348452428618E-4 62-63 0.27018970102751505 5.403737731615598E-4 1.9138237799471908E-4 64-65 0.2744423300444272 5.60074900308075E-4 1.9419682472993554E-4 66-67 0.27897358928812566 5.657037937785079E-4 2.0545461167080137E-4 68-69 0.28344855959711984 5.825904741898066E-4 2.1952684534688366E-4 70-71 0.2882303046002526 6.135493882771877E-4 2.1952684534688366E-4 72-73 0.2936903312665725 6.191782817476205E-4 2.307846322877495E-4 74-75 0.29941210147926756 6.388794088941357E-4 2.3359907902296593E-4 76-77 0.3053674707709856 6.670238762463003E-4 2.420424192286153E-4 78-79 0.31210244180835855 6.754672164519497E-4 2.5330020616948115E-4 80-81 0.32048949307930363 6.979827903336814E-4 2.589290996399141E-4 82-83 0.3307622236628437 7.064261305393307E-4 2.870735669920786E-4 84-85 0.3427461378613954 7.092405772745472E-4 2.9833135393294446E-4 86-87 0.35738407533125616 7.317561511562789E-4 3.0396024740337734E-4 88-89 0.3749377796188012 7.655295119788764E-4 3.152180343442432E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAAT 5640 0.0 12.390031 1 CGATTTA 3530 0.0 11.297943 4 CCGAGAT 15335 0.0 10.663721 1 CGAATTA 5930 0.0 10.569156 14-15 CTCGGGT 9525 0.0 10.480657 1 CGGAAAT 5475 0.0 10.4191265 1 CTCGATT 9480 0.0 10.3799715 1 CTCGAAT 7960 0.0 10.271871 1 CTGGAAT 15295 0.0 10.256487 1 CTCGTTT 7210 0.0 10.021726 1 GTTTGGT 12950 0.0 9.837828 1 CTCGGAT 9245 0.0 9.821112 1 CGGAGAT 16425 0.0 9.6376915 1 CGGGGAT 8405 0.0 9.614914 1 CGACGAT 13715 0.0 9.601037 1 GTGCACA 8660 0.0 9.484553 6 CTTGGAT 21245 0.0 9.420187 1 CGACAAT 6465 0.0 9.411862 1 CCGAATT 4215 0.0 9.360837 1 CCGGAAT 6265 0.0 9.257056 1 >>END_MODULE