FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n01 mx24-1.3410000000ca51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n01 mx24-1.3410000000ca51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17765481
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT56400.012.3900311
CGATTTA35300.011.2979434
CCGAGAT153350.010.6637211
CGAATTA59300.010.56915614-15
CTCGGGT95250.010.4806571
CGGAAAT54750.010.41912651
CTCGATT94800.010.37997151
CTCGAAT79600.010.2718711
CTGGAAT152950.010.2564871
CTCGTTT72100.010.0217261
GTTTGGT129500.09.8378281
CTCGGAT92450.09.8211121
CGGAGAT164250.09.63769151
CGGGGAT84050.09.6149141
CGACGAT137150.09.6010371
GTGCACA86600.09.4845536
CTTGGAT212450.09.4201871
CGACAAT64650.09.4118621
CCGAATT42150.09.3608371
CCGGAAT62650.09.2570561
Produced by FastQC (version 0.11.2)