FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n01 mx12-2.3410000000cd84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n01 mx12-2.3410000000cd84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21362566
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA75650.015.1253214-15
CGCGTAT21500.015.0357771
TATGCCG99200.013.76872346-47
CGTATGC102950.013.24438244-45
CACACGT112800.012.60599512-13
GCGTATT26100.012.5518172
CTCGTAT111900.012.20802642-43
CTCGGTT90250.011.9573271
CTCGGGT127150.011.9109657
CTCGTTT93500.011.8467641
CGTCTGA122650.011.74861416-17
CTCGAAT96850.011.436991
TTAGGTG101950.011.43270718-19
AATTAGG105550.011.20047916-17
CTGGGAT139850.011.0478131
GAGTGTC107600.010.96701324-25
CGATGTA127900.010.92284634-35
CTGGAAT182500.010.8364211
CGGGTCG144200.010.7660439
CGCGAAT30500.010.7548581
Produced by FastQC (version 0.11.2)