##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	C5B16ACXX l06n01 mx12-2.3410000000cd84.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21362566
Sequences flagged as poor quality	0
Sequence length	101
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81976331869495	33.0	33.0	33.0	33.0	33.0
2	32.821658034900864	33.0	33.0	33.0	33.0	33.0
3	32.74364877327939	33.0	33.0	33.0	33.0	33.0
4	36.74062624312079	37.0	37.0	37.0	37.0	37.0
5	36.752955286364006	37.0	37.0	37.0	37.0	37.0
6	36.69920659344013	37.0	37.0	37.0	37.0	37.0
7	36.720525661570804	37.0	37.0	37.0	37.0	37.0
8	36.7173553963508	37.0	37.0	37.0	37.0	37.0
9	36.71956917535094	37.0	37.0	37.0	37.0	37.0
10-11	36.700036269051196	37.0	37.0	37.0	37.0	37.0
12-13	36.69753575015286	37.0	37.0	37.0	37.0	37.0
14-15	39.13145672668723	40.0	40.0	40.0	37.0	40.0
16-17	39.11483374703208	40.0	40.0	40.0	37.0	40.0
18-19	39.084439645499515	40.0	40.0	40.0	37.0	40.0
20-21	39.07622422325109	40.0	40.0	40.0	37.0	40.0
22-23	39.04409645826255	40.0	40.0	40.0	37.0	40.0
24-25	39.01647622762172	40.0	40.0	40.0	37.0	40.0
26-27	38.95772104343645	40.0	40.0	40.0	37.0	40.0
28-29	38.917007137625696	40.0	40.0	40.0	37.0	40.0
30-31	38.86240218052457	40.0	40.0	40.0	37.0	40.0
32-33	38.790260870346756	40.0	40.0	40.0	37.0	40.0
34-35	38.70936410448071	40.0	37.0	40.0	37.0	40.0
36-37	38.628018328884274	40.0	37.0	40.0	37.0	40.0
38-39	38.530104857253576	40.0	37.0	40.0	37.0	40.0
40-41	38.417046224690424	40.0	37.0	40.0	37.0	40.0
42-43	38.299141193993265	40.0	37.0	40.0	37.0	40.0
44-45	38.1785195186758	40.0	37.0	40.0	37.0	40.0
46-47	38.05167471454506	40.0	37.0	40.0	37.0	40.0
48-49	37.917154404578554	40.0	37.0	40.0	35.0	40.0
50-51	37.772681661931436	40.0	37.0	40.0	33.0	40.0
52-53	37.595120665747736	40.0	37.0	40.0	33.0	40.0
54-55	37.41896226324123	37.0	37.0	40.0	33.0	40.0
56-57	37.243466093913995	37.0	37.0	40.0	33.0	40.0
58-59	37.07459532249075	37.0	37.0	40.0	33.0	40.0
60-61	36.89002657733158	37.0	37.0	40.0	33.0	40.0
62-63	36.69046026586881	37.0	37.0	40.0	33.0	40.0
64-65	36.505177327480226	37.0	37.0	40.0	33.0	40.0
66-67	36.280952063530194	37.0	37.0	38.5	33.0	40.0
68-69	36.07158856759062	37.0	37.0	37.0	33.0	40.0
70-71	35.85817764120658	37.0	37.0	37.0	33.0	40.0
72-73	35.622429814845276	37.0	37.0	37.0	33.0	40.0
74-75	35.404558984159486	37.0	37.0	37.0	33.0	37.0
76-77	32.753010757228324	33.0	33.0	35.0	27.0	37.0
78-79	34.90676548407153	37.0	33.0	37.0	33.0	37.0
80-81	35.12686404339254	37.0	33.0	37.0	33.0	37.0
82-83	35.09706912081629	37.0	33.0	37.0	33.0	37.0
84-85	35.016700100540355	37.0	33.0	37.0	33.0	37.0
86-87	34.95506745303912	37.0	33.0	37.0	33.0	37.0
88-89	34.88677642938587	37.0	33.0	37.0	33.0	37.0
90-91	34.813520388889614	37.0	33.0	37.0	33.0	37.0
92-93	34.73072633222058	37.0	33.0	37.0	33.0	37.0
94-95	34.66251118896485	37.0	33.0	37.0	33.0	37.0
96-97	34.5955818697061	37.0	33.0	37.0	33.0	37.0
98-99	34.55176559782191	37.0	33.0	37.0	33.0	37.0
100-101	32.76115050972809	35.0	30.0	37.0	27.5	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2306	1	0.015344945522954845
2306	2	0.05175037619151368
2306	3	0.13427150957258505
2306	4	0.1379589875113325
2306	5	0.11956172892580241
2306	6	0.12171934046455135
2306	7	0.09951577413156087
2306	8	0.1395409264010823
2306	9	0.10614707110737953
2306	10-11	0.1537313849332449
2306	12-13	0.12928710348487726
2306	14-15	0.3198536455941223
2306	16-17	0.32858272616199
2306	18-19	0.3513090711254492
2306	20-21	0.3305291383686111
2306	22-23	0.3445194505737774
2306	24-25	0.36111918052546343
2306	26-27	0.3777871949871425
2306	28-29	0.3576088933084094
2306	30-31	0.367852943642788
2306	32-33	0.3514580451353595
2306	34-35	0.39630865174557783
2306	36-37	0.3572771705931004
2306	38-39	0.3665137262303517
2306	40-41	0.37187073520500746
2306	42-43	0.360226433337445
2306	44-45	0.3719368937756755
2306	46-47	0.4146516663125439
2306	48-49	0.38311255515355214
2306	50-51	0.3960440677771331
2306	52-53	0.40960819448777386
2306	54-55	0.40898047335154786
2306	56-57	0.38865792168655844
2306	58-59	0.3916784897106851
2306	60-61	0.36488077528171203
2306	62-63	0.3998681751988329
2306	64-65	0.3609948819627533
2306	66-67	0.4097093557101559
2306	68-69	0.4004514244449524
2306	70-71	0.35041189785134463
2306	72-73	0.3173818345144852
2306	74-75	0.3590157276031718
2306	76-77	0.004163302937286062
2306	78-79	0.34057925549806356
2306	80-81	0.41183492415097334
2306	82-83	0.40422503260090537
2306	84-85	0.4789490188362322
2306	86-87	0.4420804099807256
2306	88-89	0.356919576273377
2306	90-91	0.39423148123429286
2306	92-93	0.40048221381407956
2306	94-95	0.497503303875348
2306	96-97	0.4270641039106522
2306	98-99	0.37921441274319534
2306	100-101	0.6736519654466449
2307	1	0.029486176338743064
2307	2	0.03967367116512577
2307	3	0.1341703438195978
2307	4	0.13030408993763842
2307	5	0.13472772674036548
2307	6	0.1660788138804392
2307	7	0.14058800724426845
2307	8	0.16251948759224888
2307	9	0.14765826801463788
2307	10-11	0.1272061568260554
2307	12-13	0.1563410730854855
2307	14-15	0.3676663619853713
2307	16-17	0.35353577131126457
2307	18-19	0.3835083858141459
2307	20-21	0.37535335733207376
2307	22-23	0.35998869973091985
2307	24-25	0.3657545709180354
2307	26-27	0.3733531645789938
2307	28-29	0.41277145861590725
2307	30-31	0.3905371975616063
2307	32-33	0.36005798118495846
2307	34-35	0.37000054655991477
2307	36-37	0.3787048828323023
2307	38-39	0.38155922804916287
2307	40-41	0.3652930151814857
2307	42-43	0.3542645426059252
2307	44-45	0.4002580887568925
2307	46-47	0.410953114146821
2307	48-49	0.4021554582736684
2307	50-51	0.4095003091108538
2307	52-53	0.43595730091869456
2307	54-55	0.43284943707566725
2307	56-57	0.4522527839648802
2307	58-59	0.43939031055336386
2307	60-61	0.39605092076463677
2307	62-63	0.4215583591637255
2307	64-65	0.42584982893832546
2307	66-67	0.3780193551199602
2307	68-69	0.4525600755285737
2307	70-71	0.4148769610864633
2307	72-73	0.40455330539567314
2307	74-75	0.41864570722793815
2307	76-77	0.20700888650963378
2307	78-79	0.4469699758400054
2307	80-81	0.4336024169428967
2307	82-83	0.5147281125670844
2307	84-85	0.49398622021783467
2307	86-87	0.4842331147088501
2307	88-89	0.52113225339086
2307	90-91	0.5434951650545443
2307	92-93	0.4511134083265418
2307	94-95	0.4923400251067136
2307	96-97	0.5065550503013654
2307	98-99	0.5308227271946038
2307	100-101	0.6226567023428231
2201	1	0.022232626553709167
2201	2	0.05120845148144326
2201	3	0.11457332224508576
2201	4	0.10720830391330338
2201	5	0.10976187401600157
2201	6	0.13191053156194243
2201	7	0.12061435638370455
2201	8	0.1143845485948134
2201	9	0.10975839799034048
2201	10-11	0.11797795067936079
2201	12-13	0.11373379343202572
2201	14-15	0.2469589529648104
2201	16-17	0.21823705593450882
2201	18-19	0.2319256649137813
2201	20-21	0.24992421640308748
2201	22-23	0.2626662888937048
2201	24-25	0.2633771765260846
2201	26-27	0.2687983881495768
2201	28-29	0.2890694430695433
2201	30-31	0.2759781848007705
2201	32-33	0.26379362872948064
2201	34-35	0.23099516799630493
2201	36-37	0.26166517167575165
2201	38-39	0.2908244545491101
2201	40-41	0.3028800975810526
2201	42-43	0.29062290378418965
2201	44-45	0.2970644258785953
2201	46-47	0.2572008468752358
2201	48-49	0.21786332424796484
2201	50-51	0.24131547999532188
2201	52-53	0.2819006598542799
2201	54-55	0.2900984071800323
2201	56-57	0.2652346622242874
2201	58-59	0.28259390976649
2201	60-61	0.326555940358368
2201	62-63	0.2988315949824809
2201	64-65	0.3211491290738593
2201	66-67	0.33209440112469935
2201	68-69	0.2998410765179571
2201	70-71	0.19311453919715404
2201	72-73	0.2229873010904626
2201	74-75	0.24755358728107524
2201	76-77	0.02372143958169204
2201	78-79	0.2861193658346508
2201	80-81	0.33142747943042394
2201	82-83	0.31086934362987506
2201	84-85	0.36785046194950155
2201	86-87	0.3432845847407009
2201	88-89	0.31075257453449723
2201	90-91	0.26803594936771447
2201	92-93	0.315662734276664
2201	94-95	0.38201774187167814
2201	96-97	0.3120210006038704
2201	98-99	0.34064110862286867
2201	100-101	0.30308026397315047
1101	1	0.044664761345615034
1101	2	0.05870300413702978
1101	3	0.1303768248047774
1101	4	0.11642213418561909
1101	5	0.09948744652395192
1101	6	0.13286273097129708
1101	7	0.1341676393941853
1101	8	0.13015103347025558
1101	9	0.11970628803970129
1101	10-11	0.13428759242160737
1101	12-13	0.1305475583394866
1101	14-15	0.24875535553757544
1101	16-17	0.29837466714737104
1101	18-19	0.32307754920159937
1101	20-21	0.29939938808416855
1101	22-23	0.28953359217384644
1101	24-25	0.32827423753338536
1101	26-27	0.3369757224436043
1101	28-29	0.3446267464775232
1101	30-31	0.37161499878531146
1101	32-33	0.3201709103120365
1101	34-35	0.343757737864415
1101	36-37	0.37267690014346044
1101	38-39	0.3699595601638279
1101	40-41	0.32588889528403797
1101	42-43	0.34023400502464796
1101	44-45	0.37824980883502235
1101	46-47	0.34635986524197193
1101	48-49	0.3610841750138363
1101	50-51	0.37202265708261706
1101	52-53	0.4079579503138646
1101	54-55	0.37499873233249303
1101	56-57	0.41062317826835226
1101	58-59	0.4353016955926776
1101	60-61	0.432704342508913
1101	62-63	0.4013190407671985
1101	64-65	0.4282846339583557
1101	66-67	0.3867329497036778
1101	68-69	0.43129814649407905
1101	70-71	0.43662454913300053
1101	72-73	0.4445910658307497
1101	74-75	0.43770944427038216
1101	76-77	0.3480390422977777
1101	78-79	0.48400451037654335
1101	80-81	0.4349669338731914
1101	82-83	0.47268484427909385
1101	84-85	0.5018159410125875
1101	86-87	0.4985092698732103
1101	88-89	0.5010489872693071
1101	90-91	0.4461459593363486
1101	92-93	0.5060404474180515
1101	94-95	0.5118589657548256
1101	96-97	0.4985330455699071
1101	98-99	0.5353490835940988
1101	100-101	0.5511373764331609
2304	1	0.03695147296232193
2304	2	0.049661441221559244
2304	3	0.12996788780807123
2304	4	0.1035779519610216
2304	5	0.09246934190957035
2304	6	0.12285030324468238
2304	7	0.0920612097606579
2304	8	0.10813854718180238
2304	9	0.1047445994241869
2304	10-11	0.12191946913767282
2304	12-13	0.12793875344019767
2304	14-15	0.2507044450656508
2304	16-17	0.2647779668926873
2304	18-19	0.28114023472925
2304	20-21	0.2722506239155109
2304	22-23	0.2878517247558605
2304	24-25	0.2859998135868622
2304	26-27	0.3016418939797134
2304	28-29	0.27932509568041297
2304	30-31	0.2775598129024601
2304	32-33	0.27787868769309654
2304	34-35	0.24269374346382477
2304	36-37	0.2926233438350039
2304	38-39	0.2694545892914064
2304	40-41	0.29020682623240646
2304	42-43	0.2896224047894975
2304	44-45	0.3182254961500419
2304	46-47	0.3323324938515668
2304	48-49	0.3139931886844991
2304	50-51	0.2995699750278078
2304	52-53	0.304420107086834
2304	54-55	0.32019064871983716
2304	56-57	0.3329815146630182
2304	58-59	0.36756382671111965
2304	60-61	0.3795168149266317
2304	62-63	0.3482181359034726
2304	64-65	0.34981663147605957
2304	66-67	0.30615166037751607
2304	68-69	0.3697843951945359
2304	70-71	0.37435733789414627
2304	72-73	0.3698386711138042
2304	74-75	0.44486971012683796
2304	76-77	0.28801587426660546
2304	78-79	0.4338386764240312
2304	80-81	0.4500658400959665
2304	82-83	0.43836604799625434
2304	84-85	0.5000202827063163
2304	86-87	0.4100287246037837
2304	88-89	0.49070526657626345
2304	90-91	0.46848748895965286
2304	92-93	0.4619681492583041
2304	94-95	0.4971316197134428
2304	96-97	0.40406213213577047
2304	98-99	0.47637325991252055
2304	100-101	0.47456580146847216
2202	1	0.04216797506275327
2202	2	0.04733113597671945
2202	3	0.12020616814316298
2202	4	0.10120734500693374
2202	5	0.09355503127089548
2202	6	0.09269964194664482
2202	7	0.10756767561747438
2202	8	0.10159011286479114
2202	9	0.09951539122979369
2202	10-11	0.10769982401949818
2202	12-13	0.11118926515200656
2202	14-15	0.25061115804492573
2202	16-17	0.20401021611349535
2202	18-19	0.24984996275666305
2202	20-21	0.28318046857067003
2202	22-23	0.2734703831731693
2202	24-25	0.28231934571803663
2202	26-27	0.28348370749061047
2202	28-29	0.30755511253533285
2202	30-31	0.3160306940801476
2202	32-33	0.27263046188094364
2202	34-35	0.29560556929294535
2202	36-37	0.3143295807627524
2202	38-39	0.3263519555440766
2202	40-41	0.30803838617461565
2202	42-43	0.3442830348296795
2202	44-45	0.37859189595447873
2202	46-47	0.3705400557650407
2202	48-49	0.3379978289417096
2202	50-51	0.34325320252737157
2202	52-53	0.3772420880286873
2202	54-55	0.3539856977010487
2202	56-57	0.3907282133995622
2202	58-59	0.37211674163562236
2202	60-61	0.38186213599247765
2202	62-63	0.3482108952984575
2202	64-65	0.35359718578681765
2202	66-67	0.3735933838966048
2202	68-69	0.3949089572170905
2202	70-71	0.35254326353033605
2202	72-73	0.40333853524821706
2202	74-75	0.43245628713097517
2202	76-77	0.42936401720297823
2202	78-79	0.4798954624839311
2202	80-81	0.4489448127758848
2202	82-83	0.46571067294323854
2202	84-85	0.5378558955187884
2202	86-87	0.548619257601139
2202	88-89	0.4399483313059136
2202	90-91	0.4859393989805625
2202	92-93	0.4949350200871976
2202	94-95	0.4925076378614506
2202	96-97	0.48450955668059237
2202	98-99	0.5355075821045361
2202	100-101	0.5943008284050961
1102	1	0.03578192033536709
1102	2	0.0369999976874098
1102	3	0.10491140490569961
1102	4	0.10031100697110418
1102	5	0.09303775993120666
1102	6	0.10201088327365682
1102	7	0.09532821596328489
1102	8	0.09254545289841332
1102	9	0.10852316670418816
1102	10-11	0.09138901530443633
1102	12-13	0.11039251616047352
1102	14-15	0.21855444946201885
1102	16-17	0.20440057164409353
1102	18-19	0.22043094415636233
1102	20-21	0.22993768149015636
1102	22-23	0.22954597709226476
1102	24-25	0.2303001858593845
1102	26-27	0.24465477444878303
1102	28-29	0.2390008785565314
1102	30-31	0.2144407325019344
1102	32-33	0.22214568131811774
1102	34-35	0.2364926798858633
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1213	44-45	-0.6253880011157165
1213	46-47	-0.7768369021109507
1213	48-49	-0.6679880831817187
1213	50-51	-0.6857047873431554
1213	52-53	-0.6700458087757397
1213	54-55	-0.7233842200525729
1213	56-57	-1.1228482476563926
1213	58-59	-1.0538813984390032
1213	60-61	-0.7119268115733064
1213	62-63	-0.7164802714100134
1213	64-65	-0.6765584512189662
1213	66-67	-0.6157226698458587
1213	68-69	-0.6935668066069312
1213	70-71	-0.6052653311854641
1213	72-73	-0.5457155013931043
1213	74-75	-0.5310701421550874
1213	76-77	-0.3160711175689457
1213	78-79	-0.6672780842138408
1213	80-81	-0.6625747153044301
1213	82-83	-0.6837602324747181
1213	84-85	-0.6044455700698919
1213	86-87	-0.6090640030706638
1213	88-89	-0.6246322646900566
1213	90-91	-0.6686881332695265
1213	92-93	-0.7095100339203171
1213	94-95	-0.6569986857709438
1213	96-97	-0.7153178187222053
1213	98-99	-0.615042053656552
1213	100-101	-0.289498333923774
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	7.0
7	39.0
8	409.0
9	1322.0
10	1454.0
11	979.0
12	800.0
13	809.0
14	1015.0
15	1872.0
16	3317.0
17	5617.0
18	8430.0
19	12359.0
20	17086.0
21	23548.0
22	32131.0
23	40698.0
24	52725.0
25	67718.0
26	86424.0
27	109744.0
28	139089.0
29	177502.0
30	225554.0
31	281139.0
32	368012.0
33	496914.0
34	712558.0
35	1178300.0
36	2744679.0
37	7648356.0
38	6919030.0
39	2930.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.503214174141004	12.043960367938885	9.50693126157987	57.94589419634024
2	20.7809815456101	18.247503528504293	42.78912096743955	18.18239395844606
3	23.779519538351163	23.03601369869298	24.55082703251441	28.633639730441452
4	27.794927983180784	28.33102833074746	17.183644480595472	26.690399205476282
5	28.930721839025363	29.649087025400966	21.374937138774694	20.045253996798976
6	20.804616823653113	32.21560087865849	23.37320806873107	23.606574228957328
7	19.1399572504539	14.517197980804367	41.180057676591844	25.162787092149884
8	20.798897433294027	19.228356845778052	28.19193543532347	31.780810285604456
9	22.21828594935646	18.74539322663766	31.074225820999217	27.96209500300666
10-11	25.573562367643753	27.554747818537788	20.992660829305436	25.879028984513024
12-13	23.325209535539667	22.091489518419046	27.502729017007827	27.08057192903346
14-15	24.2065207258256	23.713444407492386	25.704485771092163	26.375549095589847
16-17	25.509834750869892	24.01049095017922	24.33113358173225	26.148540717218637
18-19	25.09250189095108	24.137968322274425	24.39340704773548	26.376122739039015
20-21	25.097671555065972	24.289225860825077	24.823947782675475	25.789154801433472
22-23	25.08473183845041	24.455904534771552	24.535464353084375	25.923899273693664
24-25	24.936800663366	24.409878944317832	24.497937186010333	26.15538320630583
26-27	24.85142468372011	24.428699248957265	24.914872117890706	25.805003949431914
28-29	25.192282256878578	24.18203490357338	24.471057175994588	26.154625663553453
30-31	25.057303094408066	24.09949178290634	24.53305899672219	26.310146125963403
32-33	25.162148489791342	24.388672553541767	24.553107397095467	25.89607155957142
34-35	25.419584769200277	24.309919725092914	24.19120661158883	26.07928889411798
36-37	25.227918486009514	24.405050125604966	24.20436177695655	26.162669611428974
38-39	25.363403211546316	24.398448247687313	24.600435767984933	25.637712772781434
40-41	25.46461188291975	24.30340213246642	24.40897325502679	25.82301272958704
42-43	25.081465563931786	24.111455463660985	24.613075312888824	26.194003659518405
44-45	25.204056971271278	24.268393440688023	24.755032807332505	25.772516780708195
46-47	25.571549952565103	24.210774870346214	24.3522496301183	25.865425546970382
48-49	25.279884860909103	24.131310158969384	24.425871836127182	26.162933143994326
50-51	25.29102536575606	24.319170485552615	24.681195278115826	25.7086088705755
52-53	25.675960262018897	24.176251631973866	24.332086897898076	25.81570120810916
54-55	25.24811476993232	24.085700656174545	24.43728128530093	26.228903288592203
56-57	25.199059301334533	24.372424318868116	24.787903544309614	25.64061283548774
58-59	25.595329700565987	24.238078957659607	24.285505539306715	25.88108580246769
60-61	25.27235201500428	24.188237312261773	24.4383812341959	26.101029438538042
62-63	25.281864340885562	24.302476450997933	24.76947271781949	25.646186490297012
64-65	25.510755125464602	24.34782779735263	24.294464762366484	25.846952314816292
66-67	25.32635751869337	24.281134693500146	24.340878566399866	26.051629221406618
68-69	25.39014429494054	24.533256682012542	24.542991087527692	25.53360793551922
70-71	25.618110076954988	24.4098454240807	24.21888975774109	25.753154741223227
72-73	25.394427632768025	24.32094012381185	24.381369399821434	25.90326284359869
74-75	25.47169959362113	24.50738854818069	24.546544559308792	25.474367298889394
76-77	25.69349840895918	24.445636016701094	24.184295721722346	25.67656985261738
78-79	25.503902997922356	24.392397449524026	24.268010422039705	25.835689130513916
80-81	25.549828741713853	24.62087483329409	24.34783992675004	25.48145649824201
82-83	25.72469955506596	24.55552472172546	24.054513993144216	25.66526173006436
84-85	25.454409649929577	24.476852616055258	24.268546805748183	25.800190928266982
86-87	25.51284128067131	24.783175850819735	24.346319833223877	25.35766303528508
88-89	25.679556757226642	24.554457262385533	24.083562020988527	25.68242395939929
90-91	25.605885975170317	24.371776853353865	24.139529720328127	25.882807451147695
92-93	25.595650246394086	24.67317362101128	24.377249341787792	25.353926790806845
94-95	25.815815811130012	24.5988633310271	24.109204387892383	25.4761164699505
96-97	25.489756239957316	24.58391468515533	24.21096791462224	25.71536116026511
98-99	25.639586147056132	24.75015873562743	24.302792024403065	25.30746309291338
100-101	26.017294834894113	24.626253881615234	23.882998027905174	25.473453255585483
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	1.0
4	2.5
5	2.0
6	2.5
7	5.5
8	8.5
9	12.0
10	13.0
11	16.0
12	28.5
13	50.0
14	73.5
15	106.5
16	172.5
17	250.0
18	376.5
19	570.0
20	877.0
21	1274.5
22	1934.0
23	3078.0
24	4572.0
25	6790.0
26	10085.5
27	14688.5
28	21090.0
29	29827.0
30	40983.0
31	55619.0
32	74597.5
33	100016.5
34	133965.5
35	178547.0
36	234539.0
37	305455.5
38	389080.0
39	477182.5
40	570131.5
41	662886.0
42	746555.0
43	806837.5
44	843330.0
45	854366.0
46	843852.5
47	826052.0
48	793655.5
49	754959.0
50	720036.0
51	685016.0
52	658478.5
53	646972.0
54	649464.5
55	651594.0
56	648812.0
57	641202.5
58	619294.5
59	588472.5
60	565508.0
61	542362.0
62	514382.5
63	474917.5
64	415394.0
65	375188.0
66	355377.0
67	331652.5
68	294762.0
69	248430.5
70	209711.5
71	175278.5
72	142983.5
73	114199.0
74	89798.5
75	68684.0
76	48133.0
77	32906.5
78	22854.5
79	16313.0
80	10858.0
81	6392.0
82	3594.5
83	1915.0
84	1108.5
85	654.5
86	461.0
87	316.0
88	193.0
89	126.0
90	88.0
91	53.5
92	35.5
93	31.0
94	23.5
95	10.0
96	2.5
97	3.0
98	2.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11652158266006059
2	5.3832484356046E-4
3	0.0024716131947819376
4	0.0015634825891234227
5	1.544758246738711E-4
6	0.0
7	0.0
8	2.293731942127177E-4
9	0.0
10-11	1.1702713990444781E-5
12-13	9.59622547216472E-5
14-15	2.4107590820316246E-4
16-17	7.208871818113985E-4
18-19	0.0014043256788533738
20-21	7.208871818113985E-4
22-23	1.1000551151018093E-4
24-25	0.0
26-27	0.0
28-29	1.1936768270253675E-4
30-31	0.022761778711415097
32-33	0.035590293787740666
34-35	0.05586407550478721
36-37	0.048238587068613385
38-39	0.04106014230687456
40-41	0.056315800264818375
42-43	0.06613671784559963
44-45	0.0569032765071387
46-47	0.025275521676562634
48-49	0.01896307775011672
50-51	0.027522442762728035
52-53	0.06281548761511141
54-55	0.06543221446337485
56-57	0.06537370089342263
58-59	0.04969440468902472
60-61	0.07047608419325656
62-63	0.06303549863813177
64-65	0.045734206274658205
66-67	0.014293694867929254
68-69	0.04899224184959804
70-71	0.07974463367368882
72-73	0.07935376302640797
74-75	0.06848662281488094
76-77	0.024168444933066562
78-79	0.014153262300043917
80-81	4.493842172330796E-4
82-83	0.0035014520259410782
84-85	0.007789326432040045
86-87	1.661785386643159E-4
88-89	0.0012990012529393708
90-91	9.011089772642481E-4
92-93	0.0016126339878832906
94-95	6.834384970419751E-4
96-97	0.0
98-99	9.362171192355825E-6
100-101	1.4043256788533738E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.1362566E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.61829781670693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.71954136684543	31.55385558166835
2	14.498313293738219	12.937801207598662
3	5.20763922840652	6.970679940450239
4	2.666650012353782	4.759255376965052
5	1.7734848862628396	3.9564938414352007
6	0.9977741526404004	2.6711390577793073
7	0.776193507130421	2.4242703145177553
8	0.5683921295606265	2.0288551450726637
9	0.3481868330515803	1.3981953431656287
>10	2.2210957184216786	17.688878956874767
>50	0.13837971405033708	4.29528206284256
>100	0.07732933537192097	6.151784157162187
>500	0.0051851165601332514	1.607820089810684
>1k	0.0017566689827340843	1.239536042434932
>5k	5.698032039554049E-5	0.1774892898147795
>10k+	2.1056302996551026E-5	0.13866359240713144
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.07520164010259817	6.553519834649078E-5	0.0
2	0.07595997596917899	6.553519834649078E-5	9.362171192355825E-6
3	0.07935376302640797	7.95784551350245E-5	9.362171192355825E-6
4	0.08917936169278541	7.95784551350245E-5	9.362171192355825E-6
5	0.09107988244483364	9.830279751973616E-5	9.362171192355825E-6
6	0.09631801722695672	1.123460543082699E-4	9.362171192355825E-6
7	0.09745083994123178	1.1702713990444781E-4	9.362171192355825E-6
8	0.10033438866847737	1.2638931109680364E-4	9.362171192355825E-6
9	0.10393882457753437	1.2638931109680364E-4	1.4043256788533738E-5
10-11	0.11080597714712737	1.3809202508724842E-4	1.4043256788533738E-5
12-13	0.11839401689853177	1.6383799586622692E-4	1.872434238471165E-5
14-15	0.12591184036599348	1.7085962426049381E-4	2.8086513577067475E-5
16-17	0.13329625289396413	2.0362722343373918E-4	2.8086513577067475E-5
18-19	0.1400206323528737	2.176704802222729E-4	3.276759917324539E-5
20-21	0.14670756312701386	2.3405427980889562E-4	3.5108141971334343E-5
22-23	0.15207442776303184	2.5043807939551833E-4	3.74486847694233E-5
24-25	0.15675785390200783	2.574597077897852E-4	4.212977036560121E-5
26-27	0.1609801931097603	2.878867641649416E-4	4.212977036560121E-5
28-29	0.1644161099373549	2.995894781553864E-4	4.212977036560121E-5
30-31	0.16750328588803426	3.4171924852098763E-4	4.212977036560121E-5
32-33	0.17024406150459642	3.651246765018772E-4	4.212977036560121E-5
34-35	0.17281397749689809	3.885301044827667E-4	5.6173027154134944E-5
36-37	0.17502110935549597	4.166166180598342E-4	7.48973695388466E-5
38-39	0.17725398718487284	4.166166180598342E-4	8.425954073120242E-5
40-41	0.17939558384512422	4.212977036560121E-4	9.830279751973616E-5
42-43	0.1815184561629909	4.353409604445458E-4	1.1702713990444781E-4
44-45	0.1835968581676939	4.587463884254354E-4	1.287298538948926E-4
46-47	0.1856541952872141	4.6342747402161334E-4	1.3107039669298155E-4
48-49	0.18771387294953237	4.7747073081014707E-4	1.3809202508724842E-4
50-51	0.18990696155134174	4.961950731948588E-4	1.4745419627960424E-4
52-53	0.19196663921366003	5.196005011757483E-4	1.497947390776932E-4
54-55	0.1942346251850082	5.359843007623709E-4	1.497947390776932E-4
56-57	0.19635983804567297	5.476870147528158E-4	1.497947390776932E-4
58-59	0.19876123495651224	5.547086431470826E-4	1.497947390776932E-4
60-61	0.20123518869409227	5.687518999356164E-4	1.497947390776932E-4
62-63	0.2039806453962506	5.944978707145949E-4	1.544758246738711E-4
64-65	0.20710527003169937	6.038600419069507E-4	1.5681636747196006E-4
66-67	0.2103001109510908	6.132222130993065E-4	1.59156910270049E-4
68-69	0.21362368172437712	6.296060126859291E-4	1.7085962426049381E-4
70-71	0.21747387462723344	6.553519834649077E-4	1.7554070985667172E-4
72-73	0.22213857642382473	6.600330690610856E-4	1.7788125265476068E-4
74-75	0.22764587362772806	6.9514121103242E-4	1.7788125265476068E-4
76-77	0.23419939346237714	7.162060962152206E-4	1.7788125265476068E-4
78-79	0.2425340663663719	7.208871818113985E-4	1.7788125265476068E-4
80-81	0.25345503906225497	7.442926097922881E-4	1.872434238471165E-4
82-83	0.26795938278201226	7.46633152590377E-4	1.9426505224138336E-4
84-85	0.28612433543798066	7.536547809846439E-4	2.0596776623182814E-4
86-87	0.3089493088049441	7.700385805712666E-4	2.2001102302036187E-4
88-89	0.3376092553675434	7.957845513502451E-4	2.293731942127177E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTA	7565	0.0	15.12532	14-15
CGCGTAT	2150	0.0	15.035777	1
TATGCCG	9920	0.0	13.768723	46-47
CGTATGC	10295	0.0	13.244382	44-45
CACACGT	11280	0.0	12.605995	12-13
GCGTATT	2610	0.0	12.551817	2
CTCGTAT	11190	0.0	12.208026	42-43
CTCGGTT	9025	0.0	11.957327	1
CTCGGGT	12715	0.0	11.910965	7
CTCGTTT	9350	0.0	11.846764	1
CGTCTGA	12265	0.0	11.748614	16-17
CTCGAAT	9685	0.0	11.43699	1
TTAGGTG	10195	0.0	11.432707	18-19
AATTAGG	10555	0.0	11.200479	16-17
CTGGGAT	13985	0.0	11.047813	1
GAGTGTC	10760	0.0	10.967013	24-25
CGATGTA	12790	0.0	10.922846	34-35
CTGGAAT	18250	0.0	10.836421	1
CGGGTCG	14420	0.0	10.766043	9
CGCGAAT	3050	0.0	10.754858	1
>>END_MODULE