##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	C5B16ACXX l06n01 m48-1.3410000000cb43.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15396687
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80756457541808	33.0	33.0	33.0	33.0	33.0
2	32.8148127580953	33.0	33.0	33.0	33.0	33.0
3	32.73681182191987	33.0	33.0	33.0	33.0	33.0
4	36.73267807548468	37.0	37.0	37.0	37.0	37.0
5	36.748116916321024	37.0	37.0	37.0	37.0	37.0
6	36.69851897359477	37.0	37.0	37.0	37.0	37.0
7	36.71361150616363	37.0	37.0	37.0	37.0	37.0
8	36.7127306023692	37.0	37.0	37.0	37.0	37.0
9	36.71099600842701	37.0	37.0	37.0	37.0	37.0
10-11	36.69312271529583	37.0	37.0	37.0	37.0	37.0
12-13	36.692739678347685	37.0	37.0	37.0	37.0	37.0
14-15	39.12221427895495	40.0	40.0	40.0	37.0	40.0
16-17	39.106532691091275	40.0	40.0	40.0	37.0	40.0
18-19	39.07399556151268	40.0	40.0	40.0	37.0	40.0
20-21	39.069175855818855	40.0	40.0	40.0	37.0	40.0
22-23	39.03171406290197	40.0	40.0	40.0	37.0	40.0
24-25	38.998829066279	40.0	40.0	40.0	37.0	40.0
26-27	38.94079430854183	40.0	40.0	40.0	37.0	40.0
28-29	38.898000524398526	40.0	40.0	40.0	37.0	40.0
30-31	38.831529893411485	40.0	40.0	40.0	37.0	40.0
32-33	38.742829317761675	40.0	38.5	40.0	37.0	40.0
34-35	38.663565382604716	40.0	37.0	40.0	37.0	40.0
36-37	38.58627343012169	40.0	37.0	40.0	37.0	40.0
38-39	38.49112500630818	40.0	37.0	40.0	37.0	40.0
40-41	38.36615273142852	40.0	37.0	40.0	37.0	40.0
42-43	38.21954956933268	40.0	37.0	40.0	37.0	40.0
44-45	38.12806966199936	40.0	37.0	40.0	37.0	40.0
46-47	38.00454363981031	40.0	37.0	40.0	37.0	40.0
48-49	37.88047841071264	40.0	37.0	40.0	33.0	40.0
50-51	37.74607780881692	40.0	37.0	40.0	33.0	40.0
52-53	37.56178663630689	40.0	37.0	40.0	33.0	40.0
54-55	37.372866513425905	37.0	37.0	40.0	33.0	40.0
56-57	37.19843389035576	37.0	37.0	40.0	33.0	40.0
58-59	37.021762961083766	37.0	37.0	40.0	33.0	40.0
60-61	36.827121055328334	37.0	37.0	40.0	33.0	40.0
62-63	36.612642576938796	37.0	37.0	40.0	33.0	40.0
64-65	36.416161574239965	37.0	37.0	40.0	33.0	40.0
66-67	36.19712039349764	37.0	37.0	37.0	33.0	40.0
68-69	35.98957350370245	37.0	37.0	37.0	33.0	40.0
70-71	35.75216343619897	37.0	37.0	37.0	33.0	40.0
72-73	35.49076317522075	37.0	37.0	37.0	33.0	40.0
74-75	35.264309263414916	37.0	35.0	37.0	33.0	37.0
76-77	32.61012576926451	33.0	33.0	35.0	27.0	37.0
78-79	34.77450746384596	37.0	33.0	37.0	33.0	37.0
80-81	35.00708395903612	37.0	33.0	37.0	33.0	37.0
82-83	34.97547628265743	37.0	33.0	37.0	33.0	37.0
84-85	34.888530207829774	37.0	33.0	37.0	33.0	37.0
86-87	34.82716411654013	37.0	33.0	37.0	33.0	37.0
88-89	34.74745956711337	37.0	33.0	37.0	33.0	37.0
90-91	34.67243722626822	37.0	33.0	37.0	33.0	37.0
92-93	34.598838763170285	37.0	33.0	37.0	33.0	37.0
94-95	34.53398175854325	37.0	33.0	37.0	33.0	37.0
96-97	34.46571833927649	37.0	33.0	37.0	33.0	37.0
98-99	34.41033051461005	37.0	33.0	37.0	27.0	37.0
100-101	32.61883147329033	35.0	30.0	37.0	24.5	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2306	1	-0.004736854015717995
2306	2	0.04021060568540946
2306	3	0.1448758403924728
2306	4	0.1532010307838476
2306	5	0.14791181604950054
2306	6	0.12430282630309364
2306	7	0.12513146020727106
2306	8	0.17380624978496684
2306	9	0.12679871233883233
2306	10-11	0.16818774668322334
2306	12-13	0.15141255285612232
2306	14-15	0.32919741448582585
2306	16-17	0.33752381882394644
2306	18-19	0.3801610718195576
2306	20-21	0.3621058824386978
2306	22-23	0.365587057013677
2306	24-25	0.3889534630456737
2306	26-27	0.39888935148364624
2306	28-29	0.399931257140949
2306	30-31	0.37482119531067326
2306	32-33	0.323981827920953
2306	34-35	0.3818349451791505
2306	36-37	0.35934259265870594
2306	38-39	0.3798739546043919
2306	40-41	0.37826740310640616
2306	42-43	0.33865876540561146
2306	44-45	0.3697439706181598
2306	46-47	0.40910049565804485
2306	48-49	0.38496199748752247
2306	50-51	0.39843731840021235
2306	52-53	0.37197344654605047
2306	54-55	0.3674008957219925
2306	56-57	0.39105203746576933
2306	58-59	0.41858002992032795
2306	60-61	0.38572116304406023
2306	62-63	0.42147030862324186
2306	64-65	0.4101603885742193
2306	66-67	0.446425713195886
2306	68-69	0.4413495400783276
2306	70-71	0.43908706153820987
2306	72-73	0.4223551065744502
2306	74-75	0.4380033608979019
2306	76-77	0.11763850284120991
2306	78-79	0.4717518991498153
2306	80-81	0.5184573367132259
2306	82-83	0.5433159718976484
2306	84-85	0.5844326556387429
2306	86-87	0.5044704860393381
2306	88-89	0.44155551506884194
2306	90-91	0.5025609460040172
2306	92-93	0.43743897930220044
2306	94-95	0.5171209275549202
2306	96-97	0.4209482734135861
2306	98-99	0.40010926373377487
2306	100-101	0.7442071167823698
2307	1	0.01761023283270191
2307	2	0.05152978341524772
2307	3	0.13982862975489496
2307	4	0.13052760576721312
2307	5	0.12056572326361703
2307	6	0.1539886324036388
2307	7	0.14325119889699067
2307	8	0.12553791436850048
2307	9	0.1419272091084096
2307	10-11	0.13045851452415747
2307	12-13	0.15773304346264894
2307	14-15	0.37801589560674387
2307	16-17	0.34533769869145914
2307	18-19	0.377005831898515
2307	20-21	0.3753279980919757
2307	22-23	0.36924411041339766
2307	24-25	0.40707160766461925
2307	26-27	0.3981597771732339
2307	28-29	0.41319284198651474
2307	30-31	0.3864864776862831
2307	32-33	0.3864305068124878
2307	34-35	0.3704067967714124
2307	36-37	0.3911783538893445
2307	38-39	0.38470245873220676
2307	40-41	0.4053676264165915
2307	42-43	0.4079711304595506
2307	44-45	0.38438869879019677
2307	46-47	0.43873235840370484
2307	48-49	0.4166402007631689
2307	50-51	0.4431292603979742
2307	52-53	0.43713842203115405
2307	54-55	0.3860992821015401
2307	56-57	0.40403650904852384
2307	58-59	0.40073039058202653
2307	60-61	0.3708773673141579
2307	62-63	0.4060623393661089
2307	64-65	0.44630434923306694
2307	66-67	0.4129687819912675
2307	68-69	0.40419678767283784
2307	70-71	0.4671508687479786
2307	72-73	0.44722745657526275
2307	74-75	0.4621767427470971
2307	76-77	0.2745808914657175
2307	78-79	0.5357504611704513
2307	80-81	0.5094538974736551
2307	82-83	0.5401813273709664
2307	84-85	0.5503417943699489
2307	86-87	0.5556913593376223
2307	88-89	0.5696920884211565
2307	90-91	0.6032142089584838
2307	92-93	0.4889930133915996
2307	94-95	0.5434095137914099
2307	96-97	0.5439912167874752
2307	98-99	0.5588342060570994
2307	100-101	0.6407979605163803
2201	1	0.05751193311090219
2201	2	0.06816751342240934
2201	3	0.13477267563914097
2201	4	0.12113157732903801
2201	5	0.12252080541570365
2201	6	0.14892227552694948
2201	7	0.1288353075544677
2201	8	0.11791417671169313
2201	9	0.11763899148775181
2201	10-11	0.11911382604651521
2201	12-13	0.12068583955494461
2201	14-15	0.25708565737077294
2201	16-17	0.23046908556207057
2201	18-19	0.2305021043132811
2201	20-21	0.25935467544163515
2201	22-23	0.2685890655413985
2201	24-25	0.27304163964567607
2201	26-27	0.25012270385627033
2201	28-29	0.27596460880840823
2201	30-31	0.26575044201288023
2201	32-33	0.23457355029517402
2201	34-35	0.28141531684492094
2201	36-37	0.2636262275650125
2201	38-39	0.30301838760959043
2201	40-41	0.28261876265284513
2201	42-43	0.2835669008243329
2201	44-45	0.2724545134495102
2201	46-47	0.2569432087805765
2201	48-49	0.25837832802842087
2201	50-51	0.21316062578369355
2201	52-53	0.2920259044950697
2201	54-55	0.3217240782923483
2201	56-57	0.2758898861668655
2201	58-59	0.3049004752987159
2201	60-61	0.36919870138120103
2201	62-63	0.3145538158957848
2201	64-65	0.33190508107791317
2201	66-67	0.3124264160043069
2201	68-69	0.25102170580948524
2201	70-71	0.19569910189210304
2201	72-73	0.22373724748401713
2201	74-75	0.26695736338006526
2201	76-77	-0.013974712777589104
2201	78-79	0.2513468323477568
2201	80-81	0.29603287098864683
2201	82-83	0.32591826107862687
2201	84-85	0.402031542427828
2201	86-87	0.34424001071519683
2201	88-89	0.3343626881806614
2201	90-91	0.28083620362207995
2201	92-93	0.28299594909319126
2201	94-95	0.3406811986650311
2201	96-97	0.28896323336056184
2201	98-99	0.32860497166634417
2201	100-101	0.30126813771149585
1101	1	0.05888305677456174
1101	2	0.0667208849188583
1101	3	0.1352557170625488
1101	4	0.1307086238577142
1101	5	0.10650524952316687
1101	6	0.14536754557521903
1101	7	0.14835087608379638
1101	8	0.1390321538138224
1101	9	0.13092048237662368
1101	10-11	0.13969304926283144
1101	12-13	0.12944307411910216
1101	14-15	0.2908025015843876
1101	16-17	0.31211501209175196
1101	18-19	0.3452140644062851
1101	20-21	0.3223573196906315
1101	22-23	0.31497063035551065
1101	24-25	0.36641403646626003
1101	26-27	0.3236041877949063
1101	28-29	0.3479754701322406
1101	30-31	0.366376753080182
1101	32-33	0.3440511452606856
1101	34-35	0.3216515301034448
1101	36-37	0.35129883036532306
1101	38-39	0.35458689798439735
1101	40-41	0.3343111767858389
1101	42-43	0.38007120049325493
1101	44-45	0.40662994689152043
1101	46-47	0.37038223309377116
1101	48-49	0.39336095243765357
1101	50-51	0.3799783969060968
1101	52-53	0.400114478938427
1101	54-55	0.37903675362785094
1101	56-57	0.42031469092840723
1101	58-59	0.4277557271500072
1101	60-61	0.41591597840498906
1101	62-63	0.392007028168635
1101	64-65	0.38702162390400474
1101	66-67	0.3937984242434922
1101	68-69	0.4538576075710523
1101	70-71	0.42298004308047155
1101	72-73	0.41681397493365324
1101	74-75	0.3857893761684963
1101	76-77	0.3197935789806934
1101	78-79	0.4611980341068005
1101	80-81	0.40041957581843235
1101	82-83	0.4530732924178764
1101	84-85	0.4970215593611229
1101	86-87	0.4634398015762713
1101	88-89	0.47431572669760413
1101	90-91	0.4857704133170273
1101	92-93	0.5343641662943952
1101	94-95	0.5113007332519359
1101	96-97	0.5141491679777914
1101	98-99	0.5145390834261363
1101	100-101	0.5355855455260823
2304	1	0.05893700339142782
2304	2	0.06561140746512706
2304	3	0.1313226484330272
2304	4	0.11806245300520146
2304	5	0.09079352829662213
2304	6	0.11308186559997324
2304	7	0.13257837253893
2304	8	0.13103430613603706
2304	9	0.124348478884869
2304	10-11	0.11900159650691933
2304	12-13	0.13655943192188147
2304	14-15	0.26454009840424675
2304	16-17	0.2788192571547299
2304	18-19	0.28923903781017657
2304	20-21	0.2846340162609238
2304	22-23	0.3000846818788432
2304	24-25	0.3188374388904691
2304	26-27	0.31535941579787874
2304	28-29	0.30383823110901176
2304	30-31	0.2896937695120343
2304	32-33	0.3275073236416972
2304	34-35	0.2654389936733139
2304	36-37	0.30207006376296164
2304	38-39	0.29551375136014
2304	40-41	0.3190444535655459
2304	42-43	0.3168320556333484
2304	44-45	0.3017170097106927
2304	46-47	0.3117732367564159
2304	48-49	0.31372782484250905
2304	50-51	0.3217767021447955
2304	52-53	0.3496543392376239
2304	54-55	0.3753744572940363
2304	56-57	0.3831809359575189
2304	58-59	0.3708934526781107
2304	60-61	0.396406978834527
2304	62-63	0.4169691469897572
2304	64-65	0.4015351421194495
2304	66-67	0.3257571067390117
2304	68-69	0.41087788066040076
2304	70-71	0.4112839500533454
2304	72-73	0.4434556055871539
2304	74-75	0.4741447664424001
2304	76-77	0.26303873587082194
2304	78-79	0.49139496081237866
2304	80-81	0.49563246788979143
2304	82-83	0.44161611023564973
2304	84-85	0.5140900903473309
2304	86-87	0.4624881148977309
2304	88-89	0.5482742059695696
2304	90-91	0.5386814291114987
2304	92-93	0.4633430910878218
2304	94-95	0.4887036978525501
2304	96-97	0.4433693591618919
2304	98-99	0.5065577245687436
2304	100-101	0.47045848461598183
2202	1	0.03581504042499972
2202	2	0.055109592422361686
2202	3	0.14371125991868894
2202	4	0.11077029326344956
2202	5	0.10651439602971635
2202	6	0.11076367711515189
2202	7	0.1262017982711754
2202	8	0.12632019267110906
2202	9	0.12842382268081565
2202	10-11	0.12996396146953515
2202	12-13	0.13285449422765794
2202	14-15	0.25812233369843085
2202	16-17	0.23061799238944758
2202	18-19	0.26697577669084893
2202	20-21	0.3004021844028273
2202	22-23	0.2873989839404274
2202	24-25	0.27655476827459324
2202	26-27	0.287875174637108
2202	28-29	0.3133519842449317
2202	30-31	0.34263611727358523
2202	32-33	0.3089907929518816
2202	34-35	0.31496150447859605
2202	36-37	0.3369538007233288
2202	38-39	0.341456260992409
2202	40-41	0.2985287846158329
2202	42-43	0.2968263785135363
2202	44-45	0.3398353437694155
2202	46-47	0.35146541361947214
2202	48-49	0.29081275766535697
2202	50-51	0.33236313994780176
2202	52-53	0.34353027473570563
2202	54-55	0.3830974000883032
2202	56-57	0.41972086313737833
2202	58-59	0.3818831284738309
2202	60-61	0.40948056957078194
2202	62-63	0.39214527944439226
2202	64-65	0.38980791321751695
2202	66-67	0.3798178133739327
2202	68-69	0.38338777713996564
2202	70-71	0.4009790728190552
2202	72-73	0.4022679782498173
2202	74-75	0.35830436546343236
2202	76-77	0.37807807484720257
2202	78-79	0.43706909616987133
2202	80-81	0.4144319872619562
2202	82-83	0.43137324953905676
2202	84-85	0.5147901337207585
2202	86-87	0.4898722542595664
2202	88-89	0.41246313472399976
2202	90-91	0.4710659698709705
2202	92-93	0.4824610490186245
2202	94-95	0.4831083097789275
2202	96-97	0.4700869629526281
2202	98-99	0.4915188045235439
2202	100-101	0.5817496543417064
1102	1	0.06316816406880577
1102	2	0.06114375985384868
1102	3	0.12241694905129208
1102	4	0.1216628678692544
1102	5	0.09786851352512826
1102	6	0.10324032210475309
1102	7	0.09494635317181377
1102	8	0.09285416060532725
1102	9	0.09537411963948728
1102	10-11	0.09319013109062269
1102	12-13	0.10954594946198881
1102	14-15	0.22448998692554056
1102	16-17	0.2195653589752382
1102	18-19	0.21564359828053625
1102	20-21	0.2351071828747564
1102	22-23	0.23284155434706832
1102	24-25	0.2387640573710499
1102	26-27	0.2653980054167917
1102	28-29	0.23344118798220848
1102	30-31	0.24725082808587473
1102	32-33	0.23957777591899543
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1213	36-37	-0.7058486187000028
1213	38-39	-0.6666065263550607
1213	40-41	-0.5988235901660559
1213	42-43	-0.6371643134442948
1213	44-45	-0.6379650358991071
1213	46-47	-0.7565029710268263
1213	48-49	-0.6622184579011545
1213	50-51	-0.6442050483290203
1213	52-53	-0.5707026104787545
1213	54-55	-0.639144419535981
1213	56-57	-1.0532774851386222
1213	58-59	-1.0013807249616278
1213	60-61	-0.633959483787855
1213	62-63	-0.6547588238701536
1213	64-65	-0.600447275813714
1213	66-67	-0.5246589832636701
1213	68-69	-0.5689172017027957
1213	70-71	-0.5201607095652605
1213	72-73	-0.4829693622454201
1213	74-75	-0.4713801624788303
1213	76-77	-0.26108493917215725
1213	78-79	-0.6230562465285772
1213	80-81	-0.6016034399582324
1213	82-83	-0.6383317939345048
1213	84-85	-0.5633106084569803
1213	86-87	-0.5353900736688431
1213	88-89	-0.5641007095593764
1213	90-91	-0.5735944800459691
1213	92-93	-0.626916362151114
1213	94-95	-0.6198350250176858
1213	96-97	-0.6515355367051967
1213	98-99	-0.5665928438386345
1213	100-101	-0.22160094923713558
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	36.0
8	387.0
9	1347.0
10	1634.0
11	1067.0
12	771.0
13	787.0
14	951.0
15	1670.0
16	2943.0
17	4689.0
18	6820.0
19	9686.0
20	13458.0
21	18355.0
22	24810.0
23	31594.0
24	40406.0
25	51950.0
26	66067.0
27	83342.0
28	108527.0
29	145997.0
30	178157.0
31	203386.0
32	265190.0
33	363119.0
34	526470.0
35	865098.0
36	2010402.0
37	5616879.0
38	4748536.0
39	2154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.290020011021507	12.675114319665909	15.88042154818007	48.154444121132514
2	23.916385544359738	18.32445692626044	35.19635478436176	22.56280274501806
3	26.44308225861314	22.76974004134621	24.42968075580075	26.357496944239905
4	28.32220241824451	27.28487292336978	17.988020361566274	26.40490429681944
5	29.761593779223055	28.952944475887044	20.99019904444223	20.29526270044767
6	22.84156325318557	32.713414255937	21.626009543481658	22.819012947395763
7	20.38897069220151	16.21746288665867	39.709354356557355	23.684212064582468
8	22.509195829171716	21.184440463492912	26.115057206585274	30.1913065007501
9	23.078705178588095	20.67675338207499	29.39427813269179	26.85026330664512
10-11	25.73678684729862	28.239975033586774	20.5810991132182	25.442139005896397
12-13	23.795920223035168	22.68371255482109	26.992915678222733	26.52745154392101
14-15	24.234559389685618	24.54342517904051	25.620740349121284	25.601275082152586
16-17	25.322515480947676	24.35806469180685	24.729796438970418	25.589623388275058
18-19	25.10892029790346	24.58087699289665	25.120500878627038	25.189701830572858
20-21	25.052992287897702	24.53388025132854	25.218220539886133	25.19490692088763
22-23	24.94616530367705	24.93826424403876	24.763255610677867	25.352314841606322
24-25	24.91544122446602	24.655213163715025	24.817975451472122	25.611370160346837
26-27	24.84470278718736	24.542056500273617	24.84052656394608	25.772714148592947
28-29	25.166225932693862	24.790436760168514	24.424341245328126	25.61899606180949
30-31	25.157223082397778	24.347698757803418	24.89146894999021	25.603609209808592
32-33	24.959094932956493	24.785618335241903	24.519474697268716	25.73581203453289
34-35	24.862358805330675	24.796470261206586	24.445445939339844	25.895724994122897
36-37	24.830259890038786	24.65242894990495	24.55979986920163	25.95751129085463
38-39	25.13519423672869	24.613832044761278	24.52444165279732	25.72653206571271
40-41	25.402492817864818	24.59042211930297	24.522596573987894	25.484488488844313
42-43	24.956971321786074	24.454512695690106	25.019961439675146	25.568554542848666
44-45	25.235194010912732	24.331995980630996	24.742004111973706	25.690805896482566
46-47	25.268873350522973	24.44936658841469	24.75087127611096	25.53088878495138
48-49	25.00995041972355	24.701424071200506	24.725735770769873	25.562889738306072
50-51	24.847029985913576	24.4415754383919	24.862927361758366	25.84846721393616
52-53	25.43003819597497	24.399833821756776	24.61007172082705	25.560056261441204
54-55	25.001725519108287	24.47407412414145	24.752740585782217	25.771459770968047
56-57	24.81639278205457	24.426701068783654	25.06420825845782	25.692697890703954
58-59	25.371155075793805	24.3503137281404	24.689865307437984	25.58866588862781
60-61	25.21368787930488	24.108723098296327	24.806736698432815	25.870852323965977
62-63	25.111975793120845	24.147826179072492	25.150043565909495	25.590154461897164
64-65	25.251704582540498	24.693055256697885	24.309156851419026	25.746083309342595
66-67	24.988935161230298	24.813157932429768	24.35313606291032	25.844770843429615
68-69	25.065528340337078	24.787878051428855	24.534657323023122	25.611936285210945
70-71	25.257573293959517	24.792088688979224	24.275786717732768	25.674551299328492
72-73	25.11202058650264	24.836307104661337	24.59616525752726	25.45550705130876
74-75	25.434785660554827	24.706984218717523	24.48311480637002	25.375115314357632
76-77	25.6318963727076	24.67402981372617	24.284986357931736	25.409087455634488
78-79	25.23095109400485	24.674154582421128	24.40118359208062	25.693710731493397
80-81	25.483666879938877	24.767846457519614	24.23686230965683	25.511624352884688
82-83	25.3739927049814	24.705460741819895	24.219182459739457	25.701364093459244
84-85	25.312245551942258	24.620492716178486	24.20022908708352	25.867032644795735
86-87	25.297973793790028	24.957237467668303	24.254471230636053	25.490317507905612
88-89	25.460612483363494	24.859874659041463	24.275250903883343	25.404261953711703
90-91	25.561537379627648	24.633706054314704	24.282010347968573	25.522746218089075
92-93	25.446050628423254	24.879097788282813	24.516201985908356	25.158649597385573
94-95	25.361926178536155	24.683869708978857	24.425893570253496	25.52831054223149
96-97	25.233233617076195	24.921426278263628	24.238146167419004	25.607193937241174
98-99	25.456922235433222	24.562314048846034	24.504973781855437	25.475789933865308
100-101	25.485231870197	24.702290632617	24.325901181783575	25.486576315402427
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	4.0
2	2.5
3	1.5
4	1.5
5	3.5
6	8.5
7	7.5
8	10.5
9	18.0
10	24.5
11	32.5
12	46.0
13	66.0
14	77.5
15	103.0
16	162.5
17	261.5
18	388.0
19	568.5
20	838.5
21	1215.0
22	1729.0
23	2524.5
24	3787.0
25	5539.0
26	8019.5
27	11456.5
28	16342.0
29	23034.5
30	31178.0
31	42053.5
32	56253.0
33	74074.5
34	98879.0
35	134026.0
36	177256.5
37	235562.0
38	298514.0
39	353353.5
40	410612.5
41	468063.0
42	521786.0
43	569536.0
44	611749.0
45	624413.0
46	614869.5
47	625166.0
48	610540.5
49	580754.0
50	582492.5
51	557310.5
52	516319.0
53	490107.5
54	474123.5
55	473574.0
56	484084.5
57	490467.0
58	466104.5
59	407527.5
60	366526.5
61	338230.0
62	300565.5
63	276143.0
64	241696.0
65	216160.0
66	213786.5
67	210193.0
68	194473.5
69	167522.5
70	141595.0
71	124960.5
72	102693.5
73	80942.0
74	74579.5
75	61959.5
76	39757.5
77	24795.0
78	18346.5
79	16047.5
80	13079.5
81	8775.5
82	3970.5
83	1114.0
84	595.0
85	358.5
86	258.0
87	159.0
88	112.5
89	94.0
90	66.5
91	41.0
92	23.0
93	16.0
94	10.0
95	7.0
96	6.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11471298987892654
2	4.351585506674261E-4
3	0.0022991959244219227
4	0.0014873329567588144
5	1.6237259353262166E-4
6	0.0
7	0.0
8	2.143318234630606E-4
9	0.0
10-11	1.2989807482609734E-5
12-13	7.79388448956584E-5
14-15	2.0783691972175574E-4
16-17	6.52737826001139E-4
18-19	0.0014451160824403328
20-21	6.949547003196207E-4
22-23	1.169082673434876E-4
24-25	0.0
26-27	3.2474518706524334E-6
28-29	1.4288788230870707E-4
30-31	0.02280685448759204
32-33	0.03612465460913767
34-35	0.0557230266485251
36-37	0.04774728485420273
38-39	0.041115988134330456
40-41	0.05637251702265559
42-43	0.06590054081114984
44-45	0.05650241509748169
46-47	0.02527491790928789
48-49	0.018601404315097138
50-51	0.027340297299022834
52-53	0.06231535394594954
54-55	0.06503347116168563
56-57	0.06518934885147694
58-59	0.049146936610453926
60-61	0.07022289925098822
62-63	0.06271479052603979
64-65	0.04590922709541345
66-67	0.014298530586482665
68-69	0.04899755382440391
70-71	0.07912416482844653
72-73	0.07948463198608896
74-75	0.06809906572758152
76-77	0.02387851360490734
78-79	0.014249818808422876
80-81	5.715515292348283E-4
82-83	0.003666373161966597
84-85	0.007998473957416943
86-87	2.0134201598045085E-4
88-89	0.0013217129113555404
90-91	8.995441681707239E-4
92-93	0.0016659428096446982
94-95	7.014496040609256E-4
96-97	0.0
98-99	1.6237259353262164E-5
100-101	9.7423556119573E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.5396687E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.86060907022049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.28181729074693	30.387427038638936
2	17.42010064251159	15.281124484901953
3	5.469287372974364	7.196588260761653
4	2.57036575452143	4.5095123010618705
5	1.4164631624234125	3.1063468514710766
6	0.8776155784645548	2.3095652280581556
7	0.589547013981375	1.810052377612703
8	0.4684691929969938	1.6437875308386256
9	0.2927791813242854	1.1557325894367314
>10	1.417597672956423	10.844827806390606
>50	0.09361825336625101	2.8362406568475875
>100	0.08119181783915398	7.367921919026046
>500	0.012981202679406893	4.04474106811924
>1k	0.00787704810737408	6.127779702824275
>5k	2.1436872277810887E-4	0.6551360930972611
>10k+	7.44463835071967E-5	0.7232160909132944
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	43344	0.2815151077631181	TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG	31421	0.2040763704555402	TruSeq Adapter, Index 1 (100% over 49bp)
CTGACATGCGTGCGAGTCGACGGGTGCGGAAACCCGGAAGGCACAAGGAA	15531	0.10087235000620588	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.22819194804700518	1.2989807482609734E-5	0.0
2	0.22843875438917477	1.2989807482609734E-5	0.0
3	0.22864659130889653	1.2989807482609734E-5	6.494903741304867E-6
4	0.22927659697180308	1.2989807482609734E-5	6.494903741304867E-6
5	0.22945195937281831	1.2989807482609734E-5	6.494903741304867E-6
6	0.229828663789814	3.89694224478292E-5	6.494903741304867E-6
7	0.23004949051701837	3.89694224478292E-5	6.494903741304867E-6
8	0.23038722551156623	3.89694224478292E-5	6.494903741304867E-6
9	0.23067949617992495	5.84541336717438E-5	6.494903741304867E-6
10-11	0.2311796037680054	8.118629676631083E-5	6.494903741304867E-6
12-13	0.23214409697358918	1.0067100799022544E-4	1.2989807482609734E-5
14-15	0.23300467171931208	1.1041336360218273E-4	1.2989807482609734E-5
16-17	0.2337678229089154	1.1690826734348761E-4	1.2989807482609734E-5
18-19	0.23437834386059808	1.2340317108479248E-4	1.2989807482609734E-5
20-21	0.243750489959301	1.3314552669674979E-4	1.2989807482609734E-5
22-23	0.2520899463631364	1.363929785674022E-4	1.2989807482609734E-5
24-25	0.2526127861143115	1.4288788230870707E-4	1.6237259353262167E-5
26-27	0.2530901615392974	1.4288788230870707E-4	2.92270668358719E-5
28-29	0.25359026912737787	1.4938278605001193E-4	4.5464326189134064E-5
30-31	0.2540968716191996	1.6886749727392653E-4	4.5464326189134064E-5
32-33	0.25457749449605616	1.8185730475653625E-4	4.5464326189134064E-5
34-35	0.25503538520981817	1.8835220849784112E-4	6.494903741304867E-5
36-37	0.25541208962681383	2.2082672720436547E-4	7.144394115435353E-5
38-39	0.25581802111064544	2.2407417907501789E-4	7.144394115435353E-5
40-41	0.2563051388912433	2.3706398655762761E-4	8.443374863696326E-5
42-43	0.25673704999004004	2.6304360152284707E-4	1.0067100799022544E-4
44-45	0.25732159132675747	2.8577576461741414E-4	1.0391845986087787E-4
46-47	0.25787690559663906	2.9876557210002387E-4	1.071659117315303E-4
48-49	0.25844845712587394	2.9876557210002387E-4	1.2015571921414003E-4
50-51	0.2590070188476261	3.020130239706763E-4	1.2989807482609734E-4
52-53	0.2595883127324729	3.0850792771198115E-4	1.363929785674022E-4
54-55	0.26020208113602616	3.247451870652433E-4	1.4288788230870707E-4
56-57	0.2610334288149132	3.539722539011152E-4	1.4938278605001193E-4
58-59	0.26185828159005897	3.637146095130725E-4	1.4938278605001193E-4
60-61	0.2630500964265884	3.767044169956823E-4	1.4938278605001193E-4
62-63	0.26419319948505804	3.9943658009024925E-4	1.558776897913168E-4
64-65	0.26643718872767885	4.091789357022066E-4	1.558776897913168E-4
66-67	0.2675802917861485	4.221687431848163E-4	1.558776897913168E-4
68-69	0.26888576743815085	4.286636469261212E-4	1.558776897913168E-4
70-71	0.2702789242906607	4.6113816563264555E-4	1.6237259353262166E-4
72-73	0.2719318772928228	4.676330693739504E-4	1.6886749727392653E-4
74-75	0.27375045034038814	4.8711778059786496E-4	1.6886749727392653E-4
76-77	0.27575737559645136	4.8711778059786496E-4	1.7211494914457897E-4
78-79	0.2779104361866939	4.936126843391699E-4	1.8835220849784112E-4
80-81	0.2804402011939322	5.130973955630845E-4	2.0134201598045087E-4
82-83	0.28358048715285306	5.195922993043894E-4	2.143318234630606E-4
84-85	0.2873962431008697	5.358295586576514E-4	2.143318234630606E-4
86-87	0.2920108722090668	5.390770105283039E-4	2.143318234630606E-4
88-89	0.29741463212183245	5.747989811054807E-4	2.2732163094567033E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	12050	0.0	34.219685	46-47
CACACGT	12370	0.0	33.950134	12-13
CGTATGC	12310	0.0	33.57531	44-45
CTCGTAT	12490	0.0	31.459463	42-43
CACGTCT	13930	0.0	30.012499	14-15
ATCGGAA	17305	0.0	28.425022	2
CGTCTGA	14775	0.0	28.199455	16-17
AGATCGG	12330	0.0	27.950525	1
AGCACAC	15345	0.0	27.831528	10-11
AGTCACA	15460	0.0	27.057732	28-29
GAGCACA	18710	0.0	27.050915	9
TCGGAAG	18695	0.0	26.565466	3
AGAGCAC	19560	0.0	25.632488	8
ACATGCG	5935	0.0	25.599615	4
CATGCGT	6015	0.0	25.33758	5
CGCGTGT	4760	0.0	24.737509	3
CCAGTCA	16820	0.0	24.696344	26-27
TCTGAAC	17020	0.0	24.604742	18-19
GATCGGA	20800	0.0	23.904705	1
TCACATC	18780	0.0	22.692839	30-31
>>END_MODULE