FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n01 m48-1.3410000000cb43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n01 m48-1.3410000000cb43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15396687
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC433440.2815151077631181TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG314210.2040763704555402TruSeq Adapter, Index 1 (100% over 49bp)
CTGACATGCGTGCGAGTCGACGGGTGCGGAAACCCGGAAGGCACAAGGAA155310.10087235000620588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG120500.034.21968546-47
CACACGT123700.033.95013412-13
CGTATGC123100.033.5753144-45
CTCGTAT124900.031.45946342-43
CACGTCT139300.030.01249914-15
ATCGGAA173050.028.4250222
CGTCTGA147750.028.19945516-17
AGATCGG123300.027.9505251
AGCACAC153450.027.83152810-11
AGTCACA154600.027.05773228-29
GAGCACA187100.027.0509159
TCGGAAG186950.026.5654663
AGAGCAC195600.025.6324888
ACATGCG59350.025.5996154
CATGCGT60150.025.337585
CGCGTGT47600.024.7375093
CCAGTCA168200.024.69634426-27
TCTGAAC170200.024.60474218-19
GATCGGA208000.023.9047051
TCACATC187800.022.69283930-31
Produced by FastQC (version 0.11.2)