##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	C5B16ACXX l06n01 m12-2.3410000000cc6b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21890980
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81477462406891	33.0	33.0	33.0	33.0	33.0
2	32.81748788770535	33.0	33.0	33.0	33.0	33.0
3	32.73594681462411	33.0	33.0	33.0	33.0	33.0
4	36.73573197728014	37.0	37.0	37.0	37.0	37.0
5	36.75075236467257	37.0	37.0	37.0	37.0	37.0
6	36.6986057271077	37.0	37.0	37.0	37.0	37.0
7	36.717428730920226	37.0	37.0	37.0	37.0	37.0
8	36.71565923499085	37.0	37.0	37.0	37.0	37.0
9	36.716335495258775	37.0	37.0	37.0	37.0	37.0
10-11	36.69673708075198	37.0	37.0	37.0	37.0	37.0
12-13	36.694065729355195	37.0	37.0	37.0	37.0	37.0
14-15	39.1227776234778	40.0	40.0	40.0	37.0	40.0
16-17	39.108999254487465	40.0	40.0	40.0	37.0	40.0
18-19	39.07871166571803	40.0	40.0	40.0	37.0	40.0
20-21	39.073264490671505	40.0	40.0	40.0	37.0	40.0
22-23	39.03866866627259	40.0	40.0	40.0	37.0	40.0
24-25	39.010198789638466	40.0	40.0	40.0	37.0	40.0
26-27	38.95227198599606	40.0	40.0	40.0	37.0	40.0
28-29	38.90749710611402	40.0	40.0	40.0	37.0	40.0
30-31	38.851604176697435	40.0	40.0	40.0	37.0	40.0
32-33	38.78133982580954	40.0	40.0	40.0	37.0	40.0
34-35	38.70238819824421	40.0	37.0	40.0	37.0	40.0
36-37	38.621610521776546	40.0	37.0	40.0	37.0	40.0
38-39	38.51954560736888	40.0	37.0	40.0	37.0	40.0
40-41	38.40816646856376	40.0	37.0	40.0	37.0	40.0
42-43	38.282563252079164	40.0	37.0	40.0	37.0	40.0
44-45	38.173258574992985	40.0	37.0	40.0	37.0	40.0
46-47	38.04867550470559	40.0	37.0	40.0	37.0	40.0
48-49	37.92202441370829	40.0	37.0	40.0	35.0	40.0
50-51	37.78508461019105	40.0	37.0	40.0	33.0	40.0
52-53	37.606673547735184	40.0	37.0	40.0	33.0	40.0
54-55	37.43451293637836	37.0	37.0	40.0	33.0	40.0
56-57	37.269445680366985	37.0	37.0	40.0	33.0	40.0
58-59	37.102991437569266	37.0	37.0	40.0	33.0	40.0
60-61	36.92545450226531	37.0	37.0	40.0	33.0	40.0
62-63	36.72748899318349	37.0	37.0	40.0	33.0	40.0
64-65	36.529294759759495	37.0	37.0	40.0	33.0	40.0
66-67	36.31356944732488	37.0	37.0	38.5	33.0	40.0
68-69	36.105300288063845	37.0	37.0	37.0	33.0	40.0
70-71	35.86699965008419	37.0	37.0	37.0	33.0	40.0
72-73	35.61293681233093	37.0	37.0	37.0	33.0	40.0
74-75	35.376025422342906	37.0	37.0	37.0	33.0	37.0
76-77	32.756706917643704	33.0	33.0	35.0	27.0	37.0
78-79	34.876073592867925	37.0	33.0	37.0	33.0	37.0
80-81	35.088515840770945	37.0	33.0	37.0	33.0	37.0
82-83	35.050751176968774	37.0	33.0	37.0	33.0	37.0
84-85	34.965090987246796	37.0	33.0	37.0	33.0	37.0
86-87	34.90612921395022	37.0	33.0	37.0	33.0	37.0
88-89	34.83415007002884	37.0	33.0	37.0	33.0	37.0
90-91	34.75958349055182	37.0	33.0	37.0	33.0	37.0
92-93	34.67937209754885	37.0	33.0	37.0	33.0	37.0
94-95	34.61102259926234	37.0	33.0	37.0	33.0	37.0
96-97	34.5441058371987	37.0	33.0	37.0	33.0	37.0
98-99	34.500510301503176	37.0	33.0	37.0	33.0	37.0
100-101	32.72356066288489	35.0	30.0	37.0	24.5	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2306	1	0.011152613435569947
2306	2	0.04888157246085001
2306	3	0.14183143719164093
2306	4	0.14281232808953348
2306	5	0.12993406760000425
2306	6	0.12964505957904038
2306	7	0.12736359152234655
2306	8	0.14312567610364368
2306	9	0.10894192011085835
2306	10-11	0.15797989040288485
2306	12-13	0.13538384967583994
2306	14-15	0.3005815788169528
2306	16-17	0.3157230828407265
2306	18-19	0.3498737440552091
2306	20-21	0.3279812031169911
2306	22-23	0.3175443615528124
2306	24-25	0.3288694586756762
2306	26-27	0.3249440043721421
2306	28-29	0.34394021802794583
2306	30-31	0.3521521673674357
2306	32-33	0.2932331854695889
2306	34-35	0.3284186623556735
2306	36-37	0.31429756753991
2306	38-39	0.36803187730424725
2306	40-41	0.3544675647237199
2306	42-43	0.33839820151057154
2306	44-45	0.31504105265624816
2306	46-47	0.32475527028879014
2306	48-49	0.3188814183216735
2306	50-51	0.31465056092250876
2306	52-53	0.3703869419384347
2306	54-55	0.3351133664226822
2306	56-57	0.29981120840724174
2306	58-59	0.3221729299676568
2306	60-61	0.3185488232619136
2306	62-63	0.30816377442753407
2306	64-65	0.2822748398335122
2306	66-67	0.33132078962657374
2306	68-69	0.34376691906555834
2306	70-71	0.31537043569129963
2306	72-73	0.25509429741637035
2306	74-75	0.2965211698861694
2306	76-77	-0.061591878302060366
2306	78-79	0.29384721671554814
2306	80-81	0.3399876917138869
2306	82-83	0.34003492865668505
2306	84-85	0.43685839957242933
2306	86-87	0.35775892860379344
2306	88-89	0.2995504752306317
2306	90-91	0.3558061863848181
2306	92-93	0.3824383466952739
2306	94-95	0.4191395484695306
2306	96-97	0.2922067582165795
2306	98-99	0.30691338954109426
2306	100-101	0.5834578312802989
2307	1	0.04167091200037021
2307	2	0.0707802949584746
2307	3	0.14719172557333593
2307	4	0.12874281619106398
2307	5	0.12741880132087147
2307	6	0.15515851403603875
2307	7	0.14636077732637887
2307	8	0.15185964441353406
2307	9	0.14047181937019815
2307	10-11	0.13925201392552822
2307	12-13	0.16295143651328914
2307	14-15	0.36474545645184975
2307	16-17	0.3758836513838091
2307	18-19	0.39124300137589074
2307	20-21	0.386758317538586
2307	22-23	0.360615871907882
2307	24-25	0.3905348985877666
2307	26-27	0.39415880804946823
2307	28-29	0.4071743834573667
2307	30-31	0.38186903870290223
2307	32-33	0.37821697553308553
2307	34-35	0.41366604737626744
2307	36-37	0.4428260597012681
2307	38-39	0.4463830143065195
2307	40-41	0.41327026274498024
2307	42-43	0.42379714838895666
2307	44-45	0.4377318706603859
2307	46-47	0.4434104851490872
2307	48-49	0.48521712338051515
2307	50-51	0.48719044617376994
2307	52-53	0.4634530524763605
2307	54-55	0.47659921785326986
2307	56-57	0.47408878626945494
2307	58-59	0.4812063522144783
2307	60-61	0.4107693119506095
2307	62-63	0.4623988038277034
2307	64-65	0.44398630925481797
2307	66-67	0.440160342710449
2307	68-69	0.4751833697395398
2307	70-71	0.4762984597985991
2307	72-73	0.4813378805462847
2307	74-75	0.4715372644867699
2307	76-77	0.26146450207993155
2307	78-79	0.49939817631022976
2307	80-81	0.4865777334025694
2307	82-83	0.5273232872800335
2307	84-85	0.5215077155256864
2307	86-87	0.5191387024615537
2307	88-89	0.5331308895171816
2307	90-91	0.5187840439890223
2307	92-93	0.46556879369528303
2307	94-95	0.5306845147914458
2307	96-97	0.5445525855734132
2307	98-99	0.5427771925222089
2307	100-101	0.62943874214033
2201	1	0.048120435057875
2201	2	0.06425084244220614
2201	3	0.12313469643736141
2201	4	0.10449122918824827
2201	5	0.10730347226618164
2201	6	0.11800580411859585
2201	7	0.11021975301383691
2201	8	0.08626165510533923
2201	9	0.10839060491899488
2201	10-11	0.1166988336457706
2201	12-13	0.11958568543105486
2201	14-15	0.2546779407894988
2201	16-17	0.21576193944946453
2201	18-19	0.24107753783202668
2201	20-21	0.2525789233694553
2201	22-23	0.2331242370381048
2201	24-25	0.2427587356127745
2201	26-27	0.268247470122688
2201	28-29	0.27610034303716446
2201	30-31	0.26105317781262016
2201	32-33	0.24499937185815668
2201	34-35	0.2197291162827284
2201	36-37	0.22357967726757266
2201	38-39	0.23436921222421603
2201	40-41	0.22395105460025633
2201	42-43	0.2265025679177768
2201	44-45	0.24739248389339963
2201	46-47	0.2649870832026693
2201	48-49	0.20230250564062402
2201	50-51	0.19144390666845368
2201	52-53	0.2575234370734094
2201	54-55	0.24059749760736082
2201	56-57	0.22745443405823096
2201	58-59	0.24110296639714335
2201	60-61	0.2565896898800517
2201	62-63	0.27989412173761963
2201	64-65	0.3295887975668137
2201	66-67	0.29051278653520285
2201	68-69	0.26253904309700005
2201	70-71	0.19348601513437558
2201	72-73	0.20654032751903628
2201	74-75	0.2241776228024719
2201	76-77	-0.013986554202212176
2201	78-79	0.2475780445361977
2201	80-81	0.2976957434962557
2201	82-83	0.30187882756928985
2201	84-85	0.3644709828430237
2201	86-87	0.3350609512376579
2201	88-89	0.314106864006213
2201	90-91	0.26793544453895635
2201	92-93	0.29188574151690005
2201	94-95	0.40227133288522765
2201	96-97	0.29441915393094575
2201	98-99	0.38443464202428146
2201	100-101	0.2804336821820286
1101	1	0.03984125396265625
1101	2	0.05962371823382995
1101	3	0.1274016283263819
1101	4	0.11790754248426794
1101	5	0.10937960577687988
1101	6	0.13265360603256937
1101	7	0.13656645691365554
1101	8	0.12344116292698004
1101	9	0.1312647472796158
1101	10-11	0.12551987677537113
1101	12-13	0.1438456062617277
1101	14-15	0.2647842639211504
1101	16-17	0.2734493387325685
1101	18-19	0.3189889151776484
1101	20-21	0.29844336068335764
1101	22-23	0.2866568252019519
1101	24-25	0.3337435914608946
1101	26-27	0.3127481565405432
1101	28-29	0.33076618595017493
1101	30-31	0.3543145941062633
1101	32-33	0.33693182053148973
1101	34-35	0.34049399331974683
1101	36-37	0.34302380658525067
1101	38-39	0.3282402370613937
1101	40-41	0.30679654909844345
1101	42-43	0.3433646733855369
1101	44-45	0.3539571023638075
1101	46-47	0.31074315647265394
1101	48-49	0.32066546379050465
1101	50-51	0.3036881862093779
1101	52-53	0.31751556674381476
1101	54-55	0.29762202294846674
1101	56-57	0.33350330711135
1101	58-59	0.36522709138262854
1101	60-61	0.3475973149638989
1101	62-63	0.33249222710305304
1101	64-65	0.34746300194532154
1101	66-67	0.3447008166181291
1101	68-69	0.38063068289299906
1101	70-71	0.38586050609362843
1101	72-73	0.38443420296787423
1101	74-75	0.4083390153884707
1101	76-77	0.27817882203610367
1101	78-79	0.42691866437122883
1101	80-81	0.3808085465331885
1101	82-83	0.40236123870221974
1101	84-85	0.45287898886724065
1101	86-87	0.4294004246000682
1101	88-89	0.4369928034102557
1101	90-91	0.42039198582347836
1101	92-93	0.45052233887174253
1101	94-95	0.4907111093218788
1101	96-97	0.44788803048964354
1101	98-99	0.48442359940710844
1101	100-101	0.49144622969910756
2304	1	0.052142304722814004
2304	2	0.051695082773257184
2304	3	0.1257542187308971
2304	4	0.11612220152045438
2304	5	0.10461999893465901
2304	6	0.1268457026382066
2304	7	0.10523570943870197
2304	8	0.11244325305421654
2304	9	0.11538565836745107
2304	10-11	0.12581449312989434
2304	12-13	0.11734245451720682
2304	14-15	0.25898954727214374
2304	16-17	0.265545847123569
2304	18-19	0.2744966807270899
2304	20-21	0.269370887653416
2304	22-23	0.2783796356651962
2304	24-25	0.29266963067561136
2304	26-27	0.31147131999018995
2304	28-29	0.29242189442136635
2304	30-31	0.2608994738494985
2304	32-33	0.28462655182810437
2304	34-35	0.23898597812546996
2304	36-37	0.27849442060826135
2304	38-39	0.2817942875251802
2304	40-41	0.30857475642632437
2304	42-43	0.30304839290695895
2304	44-45	0.33843866726353866
2304	46-47	0.3439670836168176
2304	48-49	0.3229158222983912
2304	50-51	0.3406303390132095
2304	52-53	0.31750346484705005
2304	54-55	0.32774963226327003
2304	56-57	0.3539280910352218
2304	58-59	0.3913980503831809
2304	60-61	0.38507271191583214
2304	62-63	0.341293906362246
2304	64-65	0.3776753418258849
2304	66-67	0.3328680790078735
2304	68-69	0.3710447704689699
2304	70-71	0.37932885308560316
2304	72-73	0.3636874999459607
2304	74-75	0.3560302025581805
2304	76-77	0.22354972982834198
2304	78-79	0.3897433274663058
2304	80-81	0.3794493392869569
2304	82-83	0.3196926719225033
2304	84-85	0.41450723467698936
2304	86-87	0.3554487092664189
2304	88-89	0.40741350726607806
2304	90-91	0.41318128458295433
2304	92-93	0.39358271147445123
2304	94-95	0.4291500117507283
2304	96-97	0.3793846668754881
2304	98-99	0.42753355088758127
2304	100-101	0.4044832313781157
2202	1	0.041132462868411324
2202	2	0.05567533744559938
2202	3	0.12312340193125237
2202	4	0.10517847117027657
2202	5	0.09660835985113891
2202	6	0.08035298748873032
2202	7	0.10040033620110478
2202	8	0.10725663730850954
2202	9	0.10419891889529964
2202	10-11	0.10679844096718227
2202	12-13	0.11052397300390027
2202	14-15	0.2508008507904407
2202	16-17	0.22799508793436019
2202	18-19	0.2761438666186322
2202	20-21	0.2675460353182544
2202	22-23	0.2611533687318328
2202	24-25	0.2532214243209481
2202	26-27	0.24936193028245413
2202	28-29	0.288982896908621
2202	30-31	0.30263677928933674
2202	32-33	0.2603101277698556
2202	34-35	0.2659067217481237
2202	36-37	0.28674094068909994
2202	38-39	0.30032371803782354
2202	40-41	0.2571450758798548
2202	42-43	0.30220042874559283
2202	44-45	0.3329339681491703
2202	46-47	0.3474993158323727
2202	48-49	0.29617070180955807
2202	50-51	0.3212548278685503
2202	52-53	0.34062303570762964
2202	54-55	0.346693499385502
2202	56-57	0.3848725778047779
2202	58-59	0.38191365112856346
2202	60-61	0.39873888844505245
2202	62-63	0.336996694303366
2202	64-65	0.3623517515775774
2202	66-67	0.34934012590030505
2202	68-69	0.38844119416555145
2202	70-71	0.38828621939877195
2202	72-73	0.3650402762898395
2202	74-75	0.3847596888887139
2202	76-77	0.4239687342785601
2202	78-79	0.4379332059472745
2202	80-81	0.40336435542018023
2202	82-83	0.41700427777357874
2202	84-85	0.4218420870773656
2202	86-87	0.4876119936663983
2202	88-89	0.36885638511245844
2202	90-91	0.4396103236579805
2202	92-93	0.4674872131152412
2202	94-95	0.41111875407362675
2202	96-97	0.4496677130266775
2202	98-99	0.45772768618246573
2202	100-101	0.5351787482756194
1102	1	0.04046574310775952
1102	2	0.0374744963140472
1102	3	0.11851118956095519
1102	4	0.10960040095947932
1102	5	0.08873406093526626
1102	6	0.08517471556566392
1102	7	0.09456643107816376
1102	8	0.09218246568359234
1102	9	0.09960798172681251
1102	10-11	0.08660690371331725
1102	12-13	0.11013176569762351
1102	14-15	0.2039858776577148
1102	16-17	0.21516619402372328
1102	18-19	0.2257700955662827
1102	20-21	0.24153029527801806
1102	22-23	0.22610031290142274
1102	24-25	0.2143302382886887
1102	26-27	0.25407885432017707
1102	28-29	0.23770352275888484
1102	30-31	0.22537619235352935
1102	32-33	0.24484811835406362
1102	34-35	0.256582342487107
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1213	54-55	-0.6922898771517154
1213	56-57	-1.110079684283825
1213	58-59	-1.0231631868209448
1213	60-61	-0.6546680464001753
1213	62-63	-0.69799220212564
1213	64-65	-0.6184970605862716
1213	66-67	-0.5626747768635809
1213	68-69	-0.6256990370203539
1213	70-71	-0.5296352298338505
1213	72-73	-0.49281692851309344
1213	74-75	-0.4542643380881728
1213	76-77	-0.24754316084224115
1213	78-79	-0.5950120550854905
1213	80-81	-0.5979886858061434
1213	82-83	-0.6174546166882422
1213	84-85	-0.5714437810551658
1213	86-87	-0.5841122897131541
1213	88-89	-0.6044895475319905
1213	90-91	-0.5863432238070985
1213	92-93	-0.6402728999444136
1213	94-95	-0.6128196176139795
1213	96-97	-0.6865671757411675
1213	98-99	-0.6367637935906032
1213	100-101	-0.29310516948657295
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	46.0
8	349.0
9	1125.0
10	1377.0
11	913.0
12	753.0
13	781.0
14	1130.0
15	1943.0
16	3499.0
17	5827.0
18	8904.0
19	12725.0
20	17977.0
21	24590.0
22	33392.0
23	42796.0
24	55027.0
25	71294.0
26	91345.0
27	115565.0
28	147772.0
29	195269.0
30	250648.0
31	291769.0
32	374027.0
33	506036.0
34	725485.0
35	1200895.0
36	2793062.0
37	7662311.0
38	7248316.0
39	4030.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.8908279201105	12.579897229942766	11.974827290454334	52.5544475594924
2	22.188254252317996	19.129761959361712	39.0091555918893	19.672828196430988
3	24.921545546341576	23.465428328243128	24.22352292383038	27.389503201584915
4	27.865775684906712	28.523130626470923	17.42999018437541	26.181103504246963
5	28.99757954594354	29.786015525879346	21.200465909768855	20.01593901840826
6	21.310672249483577	32.66027834295221	22.894904659361984	23.134144748202228
7	19.2281935299379	15.065127280733892	41.09676679618729	24.60991239314092
8	21.327428118299025	20.08710638326836	27.240017590844573	31.345447907588042
9	22.65878914511822	19.552208261119418	30.22949634963807	27.559506244124293
10-11	25.625783499160786	28.08793485809393	20.783982807528808	25.502298835216475
12-13	23.409663992176498	22.441917643323464	27.41477322366373	26.73364514083631
14-15	24.10372675433719	24.030141512662876	25.795049855902707	26.071081877097228
16-17	25.319039344982485	24.19683224045205	24.531529394185497	25.952599020379967
18-19	24.91217096990142	24.35049138598162	24.757488728024413	25.979848916092546
20-21	25.00188948932534	24.405256254380973	25.01597300843086	25.576881247862833
22-23	24.971692646574805	24.829307355480836	24.600651273551108	25.59834872439325
24-25	24.77108836607589	24.63082283205229	24.692537748424236	25.905551053447585
26-27	24.736417116465713	24.582262296668816	24.918951205449716	25.762369381415755
28-29	25.10571721242954	24.523154502034355	24.530034057912886	25.841094227623216
30-31	24.880760787912042	24.320970342428673	24.828455773671692	25.969813095987593
32-33	24.978770809718846	24.655945456226693	24.619131818254413	25.74615191580005
34-35	25.12896306186372	24.412063203995437	24.497806617259744	25.9611671168811
36-37	25.075039971082937	24.490019209108308	24.65840654366966	25.776534276139095
38-39	25.18777924107573	24.62578736809182	24.80721437881722	25.37921901201522
40-41	25.224403746775565	24.678652006364604	24.54310762981309	25.553836617046745
42-43	24.86022477993301	24.314053320364142	24.87633570206629	25.94938619763656
44-45	25.13008863354407	24.42140741487957	24.813059364441376	25.635444587134987
46-47	25.325880029835268	24.451797052186315	24.612894523627233	25.609428394351184
48-49	25.079902340340265	24.3782745133408	24.731988546093802	25.80983460022513
50-51	25.12222927928134	24.467809148690208	24.709313642615296	25.70064792941316
52-53	25.530856963023112	24.295625400100516	24.561937788069514	25.61157984880686
54-55	24.97088361991238	24.27083803293352	24.77618932624749	25.982089020906614
56-57	25.013775520080877	24.46157766382127	24.99939718886369	25.525249627234164
58-59	25.40721814700781	24.335203083824606	24.55428569051983	25.703293078647754
60-61	25.089472379696964	24.345076808878037	24.660487232591983	25.90496357883302
62-63	25.202261644414435	24.407805495325853	24.99894411372197	25.390988746537747
64-65	25.27241698725839	24.698904052637207	24.41650181491739	25.612177145187015
66-67	25.087683409762278	24.74092912421899	24.49373862888885	25.67764883712988
68-69	25.161114218285917	24.912443749503694	24.584384742254613	25.342057289955775
70-71	25.31238945689512	24.784269576880284	24.37269234571683	25.530648620507773
72-73	25.135927829442977	24.74257031490728	24.495888677473538	25.625613178176206
74-75	25.242416282327056	24.837795462325303	24.60412100194639	25.31566725340125
76-77	25.40929722396364	24.760518742239437	24.347497088136453	25.482686945660465
78-79	25.264155054816804	24.746379280526025	24.406034471159575	25.583431193497592
80-81	25.330706850897762	24.89124749715926	24.42570650040927	25.352339151533705
82-83	25.465061608797136	24.804908292827417	24.25202891994954	25.478001178425906
84-85	25.21380575581621	24.76400191426691	24.49765055510291	25.52454177481397
86-87	25.29795197502675	24.983707874818005	24.463134161779408	25.255205988375835
88-89	25.475659559496222	24.777006994154462	24.245441296579294	25.501892149770022
90-91	25.388547615159812	24.66056323104532	24.31694148206788	25.633947671726993
92-93	25.416076288689087	24.881802206121275	24.480002569134925	25.222118936054716
94-95	25.539097393147763	24.77509243426693	24.317277376438938	25.36853279614637
96-97	25.253558771695005	24.803795901325568	24.42890861898371	25.513736707995715
98-99	25.457851299696415	24.862128701196646	24.448506239614016	25.231513759492923
100-101	25.736899466072277	24.752953020189413	24.102006771922206	25.408140741816105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	1.0
3	2.5
4	3.0
5	5.0
6	8.0
7	10.0
8	13.5
9	15.0
10	14.5
11	35.0
12	54.5
13	78.5
14	116.5
15	153.5
16	225.0
17	362.5
18	579.5
19	824.0
20	1222.0
21	1915.5
22	2865.0
23	4406.0
24	6690.5
25	10195.5
26	15275.0
27	22119.0
28	31686.5
29	44265.0
30	59679.5
31	79157.5
32	103934.0
33	134753.0
34	174261.5
35	224702.5
36	284655.5
37	357713.0
38	441807.5
39	529820.0
40	621276.0
41	709455.0
42	786009.5
43	839161.0
44	868023.5
45	871241.5
46	852889.0
47	827357.5
48	789858.5
49	747818.5
50	710617.5
51	673540.0
52	644250.0
53	628166.5
54	624026.0
55	621092.0
56	615708.0
57	607965.0
58	589306.5
59	562384.5
60	544385.0
61	528549.0
62	506718.0
63	472973.0
64	419198.5
65	381880.0
66	366671.0
67	346168.5
68	310142.5
69	264419.0
70	223719.5
71	188468.0
72	156288.0
73	126262.5
74	98325.5
75	73712.0
76	52184.0
77	36543.5
78	25246.5
79	17329.0
80	11415.5
81	6821.0
82	3876.5
83	2237.0
84	1334.0
85	783.0
86	517.5
87	345.5
88	224.0
89	155.5
90	100.5
91	77.5
92	58.0
93	35.5
94	19.0
95	11.0
96	7.5
97	4.0
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11455859902114934
2	4.385367854705454E-4
3	0.0026129483467619997
4	0.0016947619521830452
5	2.1013220970463633E-4
6	0.0
7	0.0
8	2.147003012199545E-4
9	0.0
10-11	1.1420228788295454E-5
12-13	7.537351000274999E-5
14-15	1.8957579788570451E-4
16-17	6.646573154787954E-4
18-19	0.0013544391342918407
20-21	6.600892239634772E-4
22-23	1.1420228788295454E-4
24-25	0.0
26-27	2.2840457576590907E-6
28-29	1.461789284901818E-4
30-31	0.022534395445064588
32-33	0.0355648764925097
34-35	0.056331420521146154
36-37	0.04784847457720029
38-39	0.04066515066936245
40-41	0.05558225351263397
42-43	0.06631270048211638
44-45	0.05592714442204049
46-47	0.02499659677182109
48-49	0.018039393393991496
50-51	0.026860378110070908
52-53	0.06242297055682295
54-55	0.06449688410477741
56-57	0.06537167362996084
58-59	0.049687131412115865
60-61	0.07086937176864626
62-63	0.0622356788046949
64-65	0.04548220317226547
66-67	0.01371569477474284
68-69	0.04850171166389079
70-71	0.07967893625593737
72-73	0.07875389772408545
74-75	0.06769683221125779
76-77	0.023212757035089338
78-79	0.013843601337171748
80-81	4.7736556335075E-4
82-83	0.0034808857346724544
84-85	0.007585315961185841
86-87	1.6445129455145454E-4
88-89	0.001237952800651227
90-91	8.884937997293863E-4
92-93	0.0015668553897541361
94-95	7.012020476013409E-4
96-97	0.0
98-99	1.1420228788295454E-5
100-101	9.136183030636363E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.189098E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.874221103268376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.08113598538817	36.448647346132724
2	13.75170358783335	13.71711010572419
3	4.882137278476474	7.304783822497337
4	2.5902416806233606	5.167451451612438
5	1.5271614073221325	3.8082992844581263
6	1.0786330213587145	3.2277589077918547
7	0.6200185524394572	2.1646059660745722
8	0.38758317732248115	1.546432726535096
9	0.30323217422260323	1.3611121652522615
>10	1.6167853455996128	14.214619916299014
>50	0.10268648976381509	3.4412311730720355
>100	0.05374655819226589	4.941336985885528
>500	0.0037347156943322492	1.2299259615483829
>1k	0.0011630277408768712	0.9431338559654711
>5k	9.403177781083979E-6	0.04611442657260798
>10k+	2.7594844589468987E-5	0.43743590457841625
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC	49948	0.22816703500711252	TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG	34130	0.15590896341780952	TruSeq Adapter, Index 12 (100% over 49bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.16716474091155353	5.0249006668499994E-5	0.0
2	0.1677905694491521	5.0249006668499994E-5	4.568091515318181E-6
3	0.17000609383408144	5.481709818381817E-5	4.568091515318181E-6
4	0.17604511081733207	5.481709818381817E-5	4.568091515318181E-6
5	0.1771597251470697	7.30894642450909E-5	9.136183030636363E-6
6	0.1804761595871907	9.592992182168181E-5	9.136183030636363E-6
7	0.18133496079207054	1.0049801333699999E-4	9.136183030636363E-6
8	0.1832901039606267	1.0506610485231817E-4	9.136183030636363E-6
9	0.1856563753655615	1.1420228788295454E-4	1.3704274545954543E-5
10-11	0.1902747158875482	1.4161083697486362E-4	1.3704274545954543E-5
12-13	0.195059791749844	1.5759915727847726E-4	1.5988320303613633E-5
14-15	0.20010524882851294	1.735874775820909E-4	2.740854909190909E-5
16-17	0.20518496659354674	1.8957579788570454E-4	3.197664060722727E-5
18-19	0.20976219429189558	1.987119809163409E-4	3.426068636488636E-5
20-21	0.21528501693391525	2.192683927352727E-4	3.654473212254545E-5
22-23	0.2189075135055626	2.421088503118636E-4	3.654473212254545E-5
24-25	0.2220252359647672	2.695173994037727E-4	3.654473212254545E-5
26-27	0.22489399743638705	2.9007381122270453E-4	3.654473212254545E-5
28-29	0.22734934662587059	3.060621315263181E-4	3.882877788020454E-5
30-31	0.2297041978020171	3.220504518299318E-4	4.5680915153181815E-5
32-33	0.23154285463693267	3.540270924371591E-4	5.481709818381817E-5
34-35	0.23341805620397077	3.654473212254545E-4	6.395328121445453E-5
36-37	0.23496892327342128	4.156963278939545E-4	7.30894642450909E-5
38-39	0.23664769690530074	4.2026441940927267E-4	7.30894642450909E-5
40-41	0.23818029160868998	4.225484651669318E-4	8.450969303338635E-5
42-43	0.23977683959329366	4.5224106001649994E-4	9.364587606402272E-5
44-45	0.24133684284577483	4.7051342607777266E-4	1.0278205909465908E-4
46-47	0.2428694375491641	4.7964960910840907E-4	1.0506610485231817E-4
48-49	0.2444157365270993	4.887857921390454E-4	1.0506610485231817E-4
50-51	0.24606938565564446	5.207624327462727E-4	1.0963419636763635E-4
52-53	0.24776871569934283	5.413188445652045E-4	1.1420228788295452E-4
54-55	0.24964391726638094	5.618752563841362E-4	1.1877037939827272E-4
56-57	0.251338679218564	5.778635766877499E-4	1.210544251559318E-4
58-59	0.25327098192954356	5.869997597183862E-4	1.2562251667124998E-4
60-61	0.25567379806660095	6.007040342643408E-4	1.279065624289091E-4
62-63	0.25801494496820154	6.372487663868862E-4	1.279065624289091E-4
64-65	0.260376648281621	6.783615900247499E-4	1.279065624289091E-4
66-67	0.2628068729677703	6.897818188130453E-4	1.279065624289091E-4
68-69	0.26548834268726207	7.103382306319772E-4	1.301906081865682E-4
70-71	0.2685809406431325	7.605872373004772E-4	1.3247465394422727E-4
72-73	0.2724729546141835	7.628712830581363E-4	1.3932679121720455E-4
74-75	0.276764676592825	7.834276948770681E-4	1.4161083697486362E-4
76-77	0.2817164877954299	8.199724269996136E-4	1.4161083697486362E-4
78-79	0.28755222470624886	8.405288388185453E-4	1.438948827325227E-4
80-81	0.2949433967780337	8.679373879104545E-4	1.461789284901818E-4
82-83	0.3044770037705027	8.770735709410908E-4	1.5988320303613634E-4
84-85	0.31708950444429623	8.999140285176817E-4	1.6216724879379541E-4
86-87	0.3324908249881915	9.113342573059772E-4	1.713034318244318E-4
88-89	0.3518915096537478	9.524470809438408E-4	1.7815556909740908E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	15190	0.0	27.882832	46-47
CGTATGC	15655	0.0	27.069647	44-45
CACACGT	16375	0.0	26.587683	12-13
CGTCTGA	17095	0.0	25.287985	16-17
CTCGTAT	16690	0.0	25.010406	42-43
CACGTCT	19900	0.0	21.77129	14-15
TGTAATC	19490	0.0	21.588846	36-37
TAATCTC	20010	0.0	20.850264	38-39
AGCACAC	21565	0.0	20.595528	10-11
AGAGCAC	26340	0.0	20.421528	8
GAGCACA	27530	0.0	19.987171	9
ATCGGAA	25990	0.0	19.912012	2
AGTCACC	22190	0.0	19.494377	28-29
CCAGTCA	22875	0.0	19.042152	26-27
TCTGAAC	24615	0.0	17.986263	18-19
CTTGTAA	24045	0.0	17.973602	34-35
CGGAAGA	30280	0.0	17.764465	4
TCGGAAG	30255	0.0	17.214817	3
GATCGGA	30880	0.0	16.870672	1
TGCCGTC	26720	0.0	16.29854	48-49
>>END_MODULE