FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n01 m12-2.3410000000cc6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n01 m12-2.3410000000cc6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21890980
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC499480.22816703500711252TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG341300.15590896341780952TruSeq Adapter, Index 12 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG151900.027.88283246-47
CGTATGC156550.027.06964744-45
CACACGT163750.026.58768312-13
CGTCTGA170950.025.28798516-17
CTCGTAT166900.025.01040642-43
CACGTCT199000.021.7712914-15
TGTAATC194900.021.58884636-37
TAATCTC200100.020.85026438-39
AGCACAC215650.020.59552810-11
AGAGCAC263400.020.4215288
GAGCACA275300.019.9871719
ATCGGAA259900.019.9120122
AGTCACC221900.019.49437728-29
CCAGTCA228750.019.04215226-27
TCTGAAC246150.017.98626318-19
CTTGTAA240450.017.97360234-35
CGGAAGA302800.017.7644654
TCGGAAG302550.017.2148173
GATCGGA308800.016.8706721
TGCCGTC267200.016.2985448-49
Produced by FastQC (version 0.11.2)