##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l06n01 kx48-1.3410000000cbc9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16111605 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.817603087960514 33.0 33.0 33.0 33.0 33.0 2 32.82529990028927 33.0 33.0 33.0 33.0 33.0 3 32.75827281018868 33.0 33.0 33.0 33.0 33.0 4 36.74991405263473 37.0 37.0 37.0 37.0 37.0 5 36.7628580765231 37.0 37.0 37.0 37.0 37.0 6 36.71454277832655 37.0 37.0 37.0 37.0 37.0 7 36.731237390688264 37.0 37.0 37.0 37.0 37.0 8 36.73031761888403 37.0 37.0 37.0 37.0 37.0 9 36.72848757153617 37.0 37.0 37.0 37.0 37.0 10-11 36.711190598329594 37.0 37.0 37.0 37.0 37.0 12-13 36.70996893233169 37.0 37.0 37.0 37.0 37.0 14-15 39.16251614907391 40.0 40.0 40.0 37.0 40.0 16-17 39.14782686144552 40.0 40.0 40.0 37.0 40.0 18-19 39.11549314919277 40.0 40.0 40.0 37.0 40.0 20-21 39.11756876487476 40.0 40.0 40.0 37.0 40.0 22-23 39.08922053389467 40.0 40.0 40.0 37.0 40.0 24-25 39.0595184030393 40.0 40.0 40.0 37.0 40.0 26-27 39.00680084324311 40.0 40.0 40.0 37.0 40.0 28-29 38.96861346215973 40.0 40.0 40.0 37.0 40.0 30-31 38.913353790637245 40.0 40.0 40.0 37.0 40.0 32-33 38.844629166367966 40.0 40.0 40.0 37.0 40.0 34-35 38.775438697758545 40.0 40.0 40.0 37.0 40.0 36-37 38.70821982043378 40.0 37.0 40.0 37.0 40.0 38-39 38.62447021882674 40.0 37.0 40.0 37.0 40.0 40-41 38.517746090473295 40.0 37.0 40.0 37.0 40.0 42-43 38.4117588222899 40.0 37.0 40.0 37.0 40.0 44-45 38.31193617892196 40.0 37.0 40.0 37.0 40.0 46-47 38.19923896470898 40.0 37.0 40.0 37.0 40.0 48-49 38.08781431148542 40.0 37.0 40.0 37.0 40.0 50-51 37.96954695078485 40.0 37.0 40.0 37.0 40.0 52-53 37.797389366236324 40.0 37.0 40.0 33.0 40.0 54-55 37.62933202495965 40.0 37.0 40.0 33.0 40.0 56-57 37.45939082419163 37.0 37.0 40.0 33.0 40.0 58-59 37.2916209465165 37.0 37.0 40.0 33.0 40.0 60-61 37.10997169431599 37.0 37.0 40.0 33.0 40.0 62-63 36.91217482677858 37.0 37.0 40.0 33.0 40.0 64-65 36.71903081660703 37.0 37.0 40.0 33.0 40.0 66-67 36.51156141799653 37.0 37.0 40.0 33.0 40.0 68-69 36.2997522903522 37.0 37.0 37.0 33.0 40.0 70-71 36.06718498870845 37.0 37.0 37.0 33.0 40.0 72-73 35.81185170564944 37.0 37.0 37.0 33.0 40.0 74-75 35.588845835036295 37.0 37.0 37.0 33.0 38.5 76-77 32.99458890036095 33.0 33.0 37.0 27.0 37.0 78-79 35.08213129604407 37.0 33.0 37.0 33.0 37.0 80-81 35.28311341421292 37.0 37.0 37.0 33.0 37.0 82-83 35.23707302903715 37.0 37.0 37.0 33.0 37.0 84-85 35.15429654587485 37.0 37.0 37.0 33.0 37.0 86-87 35.0924358870516 37.0 33.0 37.0 33.0 37.0 88-89 35.01793797079807 37.0 33.0 37.0 33.0 37.0 90-91 34.94922492203601 37.0 33.0 37.0 33.0 37.0 92-93 34.881188807694826 37.0 33.0 37.0 33.0 37.0 94-95 34.826749724810156 37.0 33.0 37.0 33.0 37.0 96-97 34.76386976964741 37.0 33.0 37.0 33.0 37.0 98-99 34.71462765503499 37.0 33.0 37.0 33.0 37.0 100-101 33.00665892690393 35.0 33.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.0802893310371644 2306 2 0.07925073825735751 2306 3 0.14080346758253626 2306 4 0.12921397584730698 2306 5 0.12536018981420227 2306 6 0.13605610857475625 2306 7 0.1150286581904183 2306 8 0.15182383834508073 2306 9 0.12377838397704721 2306 10-11 0.16031613098724762 2306 12-13 0.13571586346237297 2306 14-15 0.31071894745194584 2306 16-17 0.34791994854179364 2306 18-19 0.38387140256271834 2306 20-21 0.33762263476421595 2306 22-23 0.36232438316569215 2306 24-25 0.3518607057502905 2306 26-27 0.35680469918438007 2306 28-29 0.36658709869505657 2306 30-31 0.35786977563175526 2306 32-33 0.31902779611434795 2306 34-35 0.3581206394415517 2306 36-37 0.3482009176499403 2306 38-39 0.3673529224193217 2306 40-41 0.3593004555315886 2306 42-43 0.3716665128363914 2306 44-45 0.3846179026635852 2306 46-47 0.40684959953041044 2306 48-49 0.3605430052870986 2306 50-51 0.381181368012399 2306 52-53 0.37776677832438565 2306 54-55 0.3601605905955765 2306 56-57 0.30098085190862633 2306 58-59 0.35361964185243266 2306 60-61 0.3152571585637247 2306 62-63 0.35784795576583406 2306 64-65 0.34741343299086225 2306 66-67 0.3615876688163837 2306 68-69 0.3752880847045077 2306 70-71 0.34357679605011526 2306 72-73 0.30130578692404697 2306 74-75 0.3129439344896454 2306 76-77 0.04189031178574254 2306 78-79 0.34053445864078924 2306 80-81 0.4421569160710632 2306 82-83 0.41961286224549355 2306 84-85 0.44792451573140113 2306 86-87 0.37272646745567073 2306 88-89 0.33806143509681874 2306 90-91 0.4121150045007482 2306 92-93 0.4271172556946894 2306 94-95 0.4287944249922475 2306 96-97 0.3597416443406303 2306 98-99 0.3532393670399827 2306 100-101 0.6426614975267384 2307 1 0.06345367149113201 2307 2 0.06486339045027734 2307 3 0.14898458575224538 2307 4 0.14496642092494483 2307 5 0.11955104879455547 2307 6 0.14725057028029198 2307 7 0.13176300462125567 2307 8 0.14364808599846413 2307 9 0.15767124249772735 2307 10-11 0.1356165610756861 2307 12-13 0.14551531299090215 2307 14-15 0.3465003454838609 2307 16-17 0.318761246824117 2307 18-19 0.3708625975746358 2307 20-21 0.3526578880005644 2307 22-23 0.3404912076150026 2307 24-25 0.37551813718904015 2307 26-27 0.3869503847345612 2307 28-29 0.4080916606142537 2307 30-31 0.368956934157211 2307 32-33 0.3679630224569408 2307 34-35 0.4095347609438207 2307 36-37 0.42910825920893103 2307 38-39 0.42014707872274926 2307 40-41 0.4260909496341938 2307 42-43 0.42657289529436326 2307 44-45 0.44231991436998896 2307 46-47 0.45823659529168737 2307 48-49 0.4652270925854296 2307 50-51 0.464835842758653 2307 52-53 0.4763621882417013 2307 54-55 0.43400265283474226 2307 56-57 0.4810807194392055 2307 58-59 0.4554262401094533 2307 60-61 0.4395912043561694 2307 62-63 0.4330692514745138 2307 64-65 0.4338375933194598 2307 66-67 0.4193676886109614 2307 68-69 0.4578674379894778 2307 70-71 0.4347249083778948 2307 72-73 0.45315963851408014 2307 74-75 0.43322608434332466 2307 76-77 0.23466032648960322 2307 78-79 0.47752069912085204 2307 80-81 0.45202854743101994 2307 82-83 0.5159787726684542 2307 84-85 0.5285420984162386 2307 86-87 0.4708808129130446 2307 88-89 0.5345281034582285 2307 90-91 0.5177560898891684 2307 92-93 0.45002325939284304 2307 94-95 0.49820856751507847 2307 96-97 0.4681968383055306 2307 98-99 0.5151143802497771 2307 100-101 0.632187366580446 2201 1 0.03194593049198602 2201 2 0.04896651408491692 2201 3 0.12115756582521442 2201 4 0.1188174401574642 2201 5 0.1181723722831336 2201 6 0.12715712985883698 2201 7 0.12620402507023698 2201 8 0.11338608992225829 2201 9 0.1255717515564001 2201 10-11 0.13749900698847028 2201 12-13 0.10508405039583124 2201 14-15 0.253093445712409 2201 16-17 0.22144193981710458 2201 18-19 0.24708439526289538 2201 20-21 0.25068102156242134 2201 22-23 0.24866992356161433 2201 24-25 0.23856316891978935 2201 26-27 0.2549981781723787 2201 28-29 0.2783851626320839 2201 30-31 0.2345170364292315 2201 32-33 0.21014740256877218 2201 34-35 0.1957377149574029 2201 36-37 0.20488304777351374 2201 38-39 0.24067363976033818 2201 40-41 0.23677396430937137 2201 42-43 0.2566435734027337 2201 44-45 0.2421597189214637 2201 46-47 0.2626734802887185 2201 48-49 0.23565813792264834 2201 50-51 0.21900101772141767 2201 52-53 0.2405932767461465 2201 54-55 0.24612618919886842 2201 56-57 0.23302040102006316 2201 58-59 0.2546105613084322 2201 60-61 0.30850258661455143 2201 62-63 0.2724860376774174 2201 64-65 0.27354675570916953 2201 66-67 0.31403408852587233 2201 68-69 0.26677041561262627 2201 70-71 0.1823585861515511 2201 72-73 0.2165379233393736 2201 74-75 0.23754329981040456 2201 76-77 -0.009709674191938689 2201 78-79 0.23830293043265272 2201 80-81 0.3024538307703395 2201 82-83 0.3109527346047045 2201 84-85 0.3803504567386682 2201 86-87 0.36175563027650526 2201 88-89 0.3066735197753587 2201 90-91 0.29104499776997983 2201 92-93 0.31176839834856196 2201 94-95 0.40088107446355536 2201 96-97 0.3107249656433311 2201 98-99 0.35735544066709934 2201 100-101 0.34342188073903657 1101 1 0.05556656629273249 1101 2 0.06267331503141094 1101 3 0.14074541597776147 1101 4 0.11825261481516236 1101 5 0.11486336621025117 1101 6 0.14124224004124386 1101 7 0.11623616967964523 1101 8 0.10823084284394469 1101 9 0.11842226779635467 1101 10-11 0.1346237556679526 1101 12-13 0.11902934704580304 1101 14-15 0.2598261733538081 1101 16-17 0.2726882421316148 1101 18-19 0.3087180119521449 1101 20-21 0.28279105451826325 1101 22-23 0.276917874293126 1101 24-25 0.30954929781403706 1101 26-27 0.2852297931525314 1101 28-29 0.306429662977429 1101 30-31 0.3490850236461114 1101 32-33 0.3139816121821397 1101 34-35 0.3293548087172695 1101 36-37 0.3400144541071626 1101 38-39 0.3190567208960928 1101 40-41 0.29399852705376617 1101 42-43 0.3271424050661409 1101 44-45 0.3745379901333834 1101 46-47 0.30383830573398285 1101 48-49 0.3185656963922483 1101 50-51 0.2947583477639526 1101 52-53 0.33105188756447745 1101 54-55 0.3049836872162146 1101 56-57 0.3366763410248339 1101 58-59 0.40098719359097146 1101 60-61 0.3958252482994098 1101 62-63 0.3760162208890492 1101 64-65 0.32710357593610695 1101 66-67 0.32972955494153666 1101 68-69 0.3511806845598784 1101 70-71 0.3360291859839606 1101 72-73 0.3489144386080625 1101 74-75 0.34983141997738443 1101 76-77 0.2961695142658982 1101 78-79 0.44162747706282346 1101 80-81 0.3748767441269152 1101 82-83 0.4204702245715666 1101 84-85 0.4833132496437855 1101 86-87 0.44489135104542044 1101 88-89 0.485167576523466 1101 90-91 0.4970496837665124 1101 92-93 0.4521600957782468 1101 94-95 0.5143637876345082 1101 96-97 0.5192376626022366 1101 98-99 0.49299845163425715 1101 100-101 0.506139458653827 2304 1 0.058776988078072634 2304 2 0.06412598531419178 2304 3 0.13539151798408255 2304 4 0.11945421197276573 2304 5 0.10224447628378641 2304 6 0.11969555401571341 2304 7 0.12082180713561996 2304 8 0.1273613025669249 2304 9 0.11942042217570759 2304 10-11 0.12088502725406869 2304 12-13 0.11330848893349099 2304 14-15 0.26253910316999907 2304 16-17 0.27178062043373785 2304 18-19 0.29573903734024753 2304 20-21 0.2803779986567676 2304 22-23 0.3024142939343193 2304 24-25 0.29418284284620455 2304 26-27 0.3207158946670745 2304 28-29 0.3014997838248732 2304 30-31 0.29954078099839876 2304 32-33 0.3195490080447101 2304 34-35 0.2567082257255464 2304 36-37 0.28870923421744266 2304 38-39 0.28699949745814024 2304 40-41 0.2930124277235109 2304 42-43 0.30036424246686266 2304 44-45 0.3032764659257836 2304 46-47 0.34052540534023024 2304 48-49 0.31233201022384804 2304 50-51 0.3084421280105758 2304 52-53 0.3081535337662231 2304 54-55 0.3167620508251403 2304 56-57 0.3319452964513303 2304 58-59 0.33034123263239934 2304 60-61 0.3459561183917472 2304 62-63 0.3309420697105807 2304 64-65 0.38987948840701137 2304 66-67 0.3176983117933503 2304 68-69 0.3808955879940541 2304 70-71 0.37208226189518 2304 72-73 0.4082421185657594 2304 74-75 0.4160980617745196 2304 76-77 0.2681219218167996 2304 78-79 0.4406786027657006 2304 80-81 0.418610757247059 2304 82-83 0.39853715025957825 2304 84-85 0.46168346857029974 2304 86-87 0.4467942976883492 2304 88-89 0.43922734195469815 2304 90-91 0.4351719369687359 2304 92-93 0.4179776814663754 2304 94-95 0.4640085203605224 2304 96-97 0.42125944917268043 2304 98-99 0.46200144580247837 2304 100-101 0.47477829448850883 2202 1 0.03039207633546681 2202 2 0.04572213924739543 2202 3 0.11045404346749876 2202 4 0.07570337780655478 2202 5 0.08897726855056476 2202 6 0.09313466199141374 2202 7 0.08912713955214002 2202 8 0.09789763393555262 2202 9 0.09166139064385703 2202 10-11 0.09166554561559082 2202 12-13 0.09529706190947707 2202 14-15 0.225195429057635 2202 16-17 0.20405535070058534 2202 18-19 0.23137939954940379 2202 20-21 0.24479188739088187 2202 22-23 0.24894205265658087 2202 24-25 0.22572477676285274 2202 26-27 0.2394069602070772 2202 28-29 0.2533554585479223 2202 30-31 0.23881467445384885 2202 32-33 0.222942329529225 2202 34-35 0.24513494842507555 2202 36-37 0.25201285364938286 2202 38-39 0.24317282440228638 2202 40-41 0.2075103607812352 2202 42-43 0.2532520513617911 2202 44-45 0.27978595418099417 2202 46-47 0.2858431136618478 2202 48-49 0.2549927573737065 2202 50-51 0.2788614304755086 2202 52-53 0.27769660284134545 2202 54-55 0.265249764522693 2202 56-57 0.28370939249931126 2202 58-59 0.3119631487983199 2202 60-61 0.30324354900416495 2202 62-63 0.28267875866216485 2202 64-65 0.28801918918118474 2202 66-67 0.3159894900073468 2202 68-69 0.30452363935371807 2202 70-71 0.3177423372345345 2202 72-73 0.3141519966432611 2202 74-75 0.3230462183150351 2202 76-77 0.35402951471064625 2202 78-79 0.4333573254566403 2202 80-81 0.35899511979677357 2202 82-83 0.3932319092930925 2202 84-85 0.41618062468309347 2202 86-87 0.4144161933495738 2202 88-89 0.3262499810975896 2202 90-91 0.4347939823262337 2202 92-93 0.4530694331942584 2202 94-95 0.4048198495641344 2202 96-97 0.3965894825865206 2202 98-99 0.43844815281302374 2202 100-101 0.554941856448977 1102 1 0.028590054261371733 1102 2 0.028637375992438763 1102 3 0.10037474233866561 1102 4 0.10844303897507501 1102 5 0.0876878906872065 1102 6 0.07549605387110603 1102 7 0.08193998929335322 1102 8 0.0943813944611378 1102 9 0.08933456678881413 1102 10-11 0.08116950999660588 1102 12-13 0.09617510864723045 1102 14-15 0.2068476293414463 1102 16-17 0.1949135333194434 1102 18-19 0.22312136998084497 1102 20-21 0.23127435786317818 1102 22-23 0.21444089464802119 1102 24-25 0.22435502385195605 1102 26-27 0.21733624232282267 1102 28-29 0.20218525864370207 1102 30-31 0.2048660104695017 1102 32-33 0.2029245524598906 1102 34-35 0.20724689398078056 1102 36-37 0.2086072885891639 1102 38-39 0.2089398083590268 1102 40-41 0.1901527276170043 1102 42-43 0.21998556215062592 1102 44-45 0.2270383095413635 1102 46-47 0.2154777989394958 1102 48-49 0.18872623236524788 1102 50-51 0.2183687381883388 1102 52-53 0.16802485048294358 1102 54-55 0.21592380047803061 1102 56-57 0.21436193657218183 1102 58-59 0.2350725959733424 1102 60-61 0.23763589675930064 1102 62-63 0.2126845875416663 1102 64-65 0.27432166007224623 1102 66-67 0.2818159109740179 1102 68-69 0.202841774598582 1102 70-71 0.2615020928403595 1102 72-73 0.2678140931892301 1102 74-75 0.23166114011146277 1102 76-77 0.07773556087293798 1102 78-79 0.2596516507756519 1102 80-81 0.26420322025072096 1102 82-83 0.2900016428869563 1102 84-85 0.3127159873400416 1102 86-87 0.29238188063390425 1102 88-89 0.30495599686595654 1102 90-91 0.2895616747991454 1102 92-93 0.3411978360475061 1102 94-95 0.3539863468128033 1102 96-97 0.30655254383567865 1102 98-99 0.33251924352943263 1102 100-101 0.3941444819438047 2305 1 0.046937511893354156 2305 2 0.050866095313601534 2305 3 0.10141703135213476 2305 4 0.08075026405764874 2305 5 0.060688453965127565 2305 6 0.08680182182447282 2305 7 0.06549297727850245 2305 8 0.04978249798683976 2305 9 0.07556743432633084 2305 10-11 0.04593595384336879 2305 12-13 0.07512247374341285 2305 14-15 0.15920448486969008 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-0.6490399812383103 1213 46-47 -0.7989450843467196 1213 48-49 -0.6875400926522488 1213 50-51 -0.6904263747867887 1213 52-53 -0.6809670254582372 1213 54-55 -0.6953234942562077 1213 56-57 -1.0893479532047436 1213 58-59 -1.0112376062043609 1213 60-61 -0.6806406183619771 1213 62-63 -0.664099278286109 1213 64-65 -0.6374694475697993 1213 66-67 -0.6079520227656161 1213 68-69 -0.6818596255257177 1213 70-71 -0.6048490832316276 1213 72-73 -0.5749660627081425 1213 74-75 -0.5290105099559241 1213 76-77 -0.2706878006160949 1213 78-79 -0.6799690481181244 1213 80-81 -0.6389661131781637 1213 82-83 -0.6663640795711956 1213 84-85 -0.6081751940705971 1213 86-87 -0.6199113702698256 1213 88-89 -0.6515735952407056 1213 90-91 -0.621970368966835 1213 92-93 -0.7023065209492074 1213 94-95 -0.6553444870125489 1213 96-97 -0.746450368843881 1213 98-99 -0.7040785365139612 1213 100-101 -0.410844010658181 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 22.0 8 226.0 9 710.0 10 898.0 11 590.0 12 469.0 13 450.0 14 656.0 15 1236.0 16 2274.0 17 3908.0 18 5946.0 19 8751.0 20 12263.0 21 16657.0 22 22504.0 23 28365.0 24 36612.0 25 46863.0 26 60008.0 27 76134.0 28 98049.0 29 129441.0 30 157798.0 31 192117.0 32 251292.0 33 344664.0 34 498186.0 35 811217.0 36 1869287.0 37 5641864.0 38 5787784.0 39 4364.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.032159699829545 12.91688701164975 15.639841438610905 49.4111118499098 2 22.482518116211885 18.32470225061099 37.16996939335889 22.022810239818227 3 25.297848257835653 22.661388110549517 25.823326644828477 26.217436986786353 4 27.471619917018934 27.538547736454966 18.86350318566614 26.126329160859957 5 28.865654520511434 29.40166053308389 22.0498892317963 19.682795714608375 6 21.7190962663248 33.11872405014895 22.903137210724818 22.25904247280144 7 19.240280530710628 16.270328126837764 41.77579452822981 22.7135968142218 8 21.55631807138941 21.139654973584836 27.862696006561244 29.44133094846452 9 22.25107926863897 20.794669432374988 30.716660444443615 26.237590854542425 10-11 24.880898733580505 29.184781068825266 21.47168520677131 24.462634990822917 12-13 22.824403082860414 23.484191866659014 28.19847668097179 25.492928369508782 14-15 23.479940029456458 25.308286870623743 26.56052621542032 24.65124688449948 16-17 24.58729316090243 25.100352251562967 25.591724965097907 24.7206296224367 18-19 24.313026873204276 25.45453653933568 25.723507853934457 24.508928733525583 20-21 24.351162324685994 25.370221184163455 25.87713601916298 24.401480471987576 22-23 24.037863426266433 25.64128680037166 25.59416854574874 24.726681227613163 24-25 24.165590578964665 25.446359937448815 25.568129308036042 24.81992017555048 26-27 24.127990352295754 25.413175782301018 25.431265227765948 25.02756863763728 28-29 24.385730031704828 25.459829850999448 25.238321531938713 24.916118585357015 30-31 24.32524245716247 25.189424168778828 25.514128256469483 24.971205117589218 32-33 24.352335691915666 25.455282346892904 25.25945750950594 24.932924451685487 34-35 24.39261258617705 25.425242064259105 25.14478986423597 25.037355485327872 36-37 24.130755919522304 25.31149031926706 25.48815369002243 25.06960007118821 38-39 24.2822939880026 25.46792031781869 25.437438921948345 24.812346772230363 40-41 24.616329954981143 25.247108744242464 25.395952318014704 24.74060898276169 42-43 24.24179496524504 25.267571818509527 25.764344510222024 24.726288706023407 44-45 24.472105215191934 25.186003787317162 25.608173053410738 24.733717944080173 46-47 24.500666489896243 25.294261606115555 25.50467883915843 24.70039306482978 48-49 24.32938836026896 25.466780564705367 25.406502052526797 24.797329022498875 50-51 23.969523339812135 25.417116893865522 25.825572259218742 24.7877875071036 52-53 24.530930748453525 25.349689736231003 25.521808518219913 24.59757099709556 54-55 24.200414434761246 25.49927128595887 25.492926946122363 24.80738733315753 56-57 23.934749864782415 25.314234733999214 25.89256881051039 24.85844659070798 58-59 24.564431077284603 25.15636527982556 25.542517862134012 24.736685780755824 60-61 24.331140756442597 24.977559790274604 25.778568833408162 24.912730619874633 62-63 24.28685012912611 25.177631465357425 25.87919221996416 24.65632618555231 64-65 24.301508508139907 25.554666886901945 25.338059077284576 24.805765527673575 66-67 24.248225613961175 25.579086918370685 25.254684334758217 24.918003132909927 68-69 24.201770636349508 25.564049378606835 25.497719211607762 24.73646077343589 70-71 24.45878009294308 25.50731470589377 25.217263963785584 24.81664123737756 72-73 24.35987147456211 25.645597944119746 25.451742613939853 24.54278796737829 74-75 24.6597918691655 25.4350427223818 25.383267616028082 24.521897792424618 76-77 24.879785493428127 25.36431568448934 25.255618986364563 24.500279835717965 78-79 24.476590271958802 25.46733186020879 25.302774705276548 24.753303162555856 80-81 24.579625956330798 25.576136867828286 25.143489769382015 24.700747406458902 82-83 24.53692677769016 25.520468772742603 25.14062902966016 24.801975419907077 84-85 24.46908450244585 25.452600505584595 25.100669532893676 24.97764545907588 86-87 24.407578959912524 25.764044520159494 25.269617453660263 24.55875906626772 88-89 24.500111535972614 25.638467410082576 25.242545741231332 24.618875312713477 90-91 24.612443420686922 25.497205280169005 25.27896302841444 24.61138827072963 92-93 24.51577508401613 25.704807120177243 25.5467786805201 24.232639115286528 94-95 24.450164308153898 25.583342824486493 25.331004354344522 24.635488513015087 96-97 24.23816869889747 25.87399269036201 25.218645193945605 24.66919341679491 98-99 24.482199289406566 25.466492969911624 25.44111374634084 24.610193994340975 100-101 24.585360960957082 25.6387259766315 25.26610013503311 24.509812927378306 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.5 5 4.0 6 7.0 7 11.0 8 14.5 9 27.5 10 52.0 11 80.5 12 107.0 13 139.0 14 195.0 15 284.5 16 433.0 17 703.0 18 1009.5 19 1288.5 20 1712.0 21 2524.0 22 3819.0 23 5700.5 24 8266.5 25 11907.5 26 16882.0 27 23681.0 28 32762.5 29 44052.0 30 57722.5 31 74279.0 32 94899.0 33 119692.5 34 151683.0 35 196184.5 36 249213.0 37 318967.5 38 391633.0 39 453371.0 40 517510.5 41 576852.0 42 627741.0 43 669324.0 44 708197.0 45 711439.0 46 685145.5 47 676889.0 48 642913.5 49 595709.5 50 582881.5 51 551312.0 52 507196.5 53 479695.0 54 456921.5 55 445322.0 56 449005.0 57 446393.0 58 415805.5 59 359790.5 60 320483.0 61 300208.0 62 267799.0 63 238658.0 64 207411.5 65 187923.5 66 182998.5 67 176943.5 68 168227.0 69 144828.0 70 115773.0 71 92129.0 72 72552.5 73 62364.5 74 58078.5 75 48107.0 76 31387.5 77 18667.5 78 12885.5 79 12672.5 80 10952.0 81 6530.0 82 2883.5 83 720.5 84 355.5 85 236.5 86 158.0 87 104.0 88 72.0 89 45.0 90 26.5 91 23.0 92 17.5 93 11.0 94 7.5 95 4.5 96 3.5 97 2.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12050320250527494 2 4.282627336010286E-4 3 0.0023647550942317666 4 0.0013530619699278874 5 1.986146010903321E-4 6 0.0 7 0.0 8 1.6758106966996772E-4 9 0.0 10-11 3.1033531420364392E-6 12-13 5.896370969869234E-5 14-15 1.7689112909607702E-4 16-17 6.703242786798709E-4 18-19 0.0013747854419221424 20-21 7.541148135148547E-4 22-23 9.310059426109316E-5 24-25 0.0 26-27 0.0 28-29 1.27237478823494E-4 30-31 0.02301446690134223 32-33 0.03652646648176889 34-35 0.057210315173441745 36-37 0.048499202903745466 38-39 0.041581828750146245 40-41 0.05666412502044334 42-43 0.06753517107699698 44-45 0.05696204692207884 46-47 0.025348188464153636 48-49 0.01890252398814395 50-51 0.027678806673822998 52-53 0.06346046840150314 54-55 0.06603314815625135 56-57 0.06643348071157405 58-59 0.050435695264376204 60-61 0.07199468954210335 62-63 0.06387321436939399 64-65 0.047264068353214964 66-67 0.014278527806509657 68-69 0.05010363647817831 70-71 0.08095096671002051 72-73 0.08069959510551555 74-75 0.0695368338536105 76-77 0.024454422759247137 78-79 0.01432818145678224 80-81 5.617069187085955E-4 82-83 0.0037240237704437265 84-85 0.00833870989265191 86-87 1.7068442281200413E-4 88-89 0.0013096150259393772 90-91 9.372126488950046E-4 92-93 0.0016416738121372762 94-95 6.827376912480165E-4 96-97 0.0 98-99 6.2067062840728784E-6 100-101 6.2067062840728784E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6111605E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.28467383743538 #Duplication Level Percentage of deduplicated Percentage of total 1 75.97842847502801 38.20550494547696 2 14.326753536193849 14.408322574336632 3 4.239039158572036 6.394761044187342 4 1.8214418469345042 3.663624367478297 5 1.0967103049161748 2.7573859988432075 6 0.5540382474623952 1.6715779540266509 7 0.4003685285562698 1.4092680611258173 8 0.2375685559738881 0.9556845880941979 9 0.20594631930976687 0.932034914506075 >10 0.9704777084719852 8.827103521881757 >50 0.08365569916279221 2.9521900565530363 >100 0.06926254638947209 7.130249857353296 >500 0.00972413331016821 3.3851174257837178 >1k 0.00628206419312935 5.849921588487873 >5k 2.2864300638520175E-4 0.8367064884235621 >10k+ 7.423251926810957E-5 0.6205466134414617 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC 39678 0.24626969193944365 TruSeq Adapter, Index 11 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.03226245926461082 1.2413412568145755E-5 6.206706284072878E-6 2 0.03232452632745155 1.2413412568145755E-5 1.2413412568145755E-5 3 0.032454867159417075 1.2413412568145755E-5 1.2413412568145755E-5 4 0.032808649417609234 1.8620118852218633E-5 1.2413412568145755E-5 5 0.032914163424438474 1.8620118852218633E-5 1.2413412568145755E-5 6 0.033137604850665095 4.965365027258302E-5 3.7240237704437266E-5 7 0.033243118857494335 6.206706284072878E-5 3.7240237704437266E-5 8 0.03336725298317579 7.448047540887453E-5 3.7240237704437266E-5 9 0.03354104075912983 7.448047540887453E-5 4.3446943988510147E-5 10-11 0.033866892839043664 7.758382855091098E-5 5.58603565566559E-5 12-13 0.03416171138753712 9.620394740312961E-5 5.58603565566559E-5 14-15 0.03456514729600186 1.0551400682923892E-4 6.827376912480165E-5 16-17 0.03487237925706346 1.1792741939738468E-4 7.448047540887453E-5 18-19 0.035111337449000274 1.2103077253942113E-4 8.689388797702029E-5 20-21 0.03558304712658981 1.3965089139163976E-4 1.0241065368720248E-4 22-23 0.035803385199674395 1.582710102438584E-4 1.0861735997127537E-4 24-25 0.0360082065070488 1.7689112909607702E-4 1.1792741939738468E-4 26-27 0.03620682110813914 1.8620118852218635E-4 1.2103077253942113E-4 28-29 0.036383712237235216 1.8930454166422278E-4 1.3965089139163976E-4 30-31 0.0365699134257574 2.0482130737440495E-4 1.427542445336762E-4 32-33 0.03678404479255791 2.1102801365847784E-4 1.427542445336762E-4 34-35 0.03697334933422213 2.2033807308458715E-4 1.8620118852218635E-4 36-37 0.037202997466732834 2.54474957646988E-4 1.9551124794829565E-4 38-39 0.037469885836947964 2.6378501707309727E-4 1.9861460109033208E-4 40-41 0.03768091385060644 2.6688837021513373E-4 2.2964813251069647E-4 42-43 0.03791987204254325 2.8240513592531593E-4 2.5137160450495154E-4 44-45 0.0381122799373495 3.04128607919571E-4 2.54474957646988E-4 46-47 0.038366754894996496 3.32058786197899E-4 2.54474957646988E-4 48-49 0.03865536673720588 3.4757555190808117E-4 2.6378501707309727E-4 50-51 0.03903397582053433 3.4757555190808117E-4 2.6688837021513373E-4 52-53 0.03943741172899907 3.5067890505011763E-4 2.6688837021513373E-4 54-55 0.039915328112872676 3.661956707602998E-4 2.6688837021513373E-4 56-57 0.04041496796874054 3.8481578961251843E-4 2.6688837021513373E-4 58-59 0.04085874746805176 4.003325553227006E-4 2.6688837021513373E-4 60-61 0.041442177858754606 4.2826273360102856E-4 2.6688837021513373E-4 62-63 0.04218698261284335 4.499862055952836E-4 2.6688837021513373E-4 64-65 0.04314591873373261 4.686063244475023E-4 2.7309507649920665E-4 66-67 0.04389693019410543 4.81019737015648E-4 2.8240513592531593E-4 68-69 0.0447038020110349 4.8412309015768447E-4 2.9171519535142526E-4 70-71 0.045802389023315807 5.08949915293976E-4 3.04128607919571E-4 72-73 0.04701269674871002 5.213633278621217E-4 3.103353142036439E-4 74-75 0.04839679225005827 5.58603565566559E-4 3.134386673456803E-4 76-77 0.05004467276847961 5.772236844187777E-4 3.289554330558625E-4 78-79 0.05163048622406023 5.958438032709964E-4 3.475755519080811E-4 80-81 0.053560771878406896 6.206706284072878E-4 3.724023770443727E-4 82-83 0.05621413881484806 6.237739815493242E-4 3.910224958965913E-4 84-85 0.05963093062423018 6.392907472595064E-4 4.003325553227006E-4 86-87 0.06349460528606554 6.517041598276521E-4 4.034359084647371E-4 88-89 0.06819618529625074 6.672209255378344E-4 4.127459678908464E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAT 3855 0.0 36.751644 1 AGTCACG 7135 0.0 32.993908 28-29 GAGCACA 14245 0.0 31.562464 9 ATCGGAA 14065 0.0 30.550278 2 TATGCCG 7975 0.0 29.695887 46-47 ACATGCG 5170 0.0 29.662113 4 ACGGCTA 8040 0.0 29.45951 32-33 CACACGT 8190 0.0 29.276 12-13 CGTATGC 8140 0.0 29.209488 44-45 AGAGCAC 15375 0.0 28.34734 8 TCGGAAG 15585 0.0 27.905746 3 GCGTATT 5140 0.0 27.896526 2 ACGTCTG 7660 0.0 27.830986 14-15 ACACGTC 7910 0.0 27.281088 12-13 CTCGTAT 8575 0.0 27.233988 42-43 TAAGTCG 5275 0.0 27.001144 9 CATGCGT 5750 0.0 26.83475 5 CACGTCT 8950 0.0 26.763885 14-15 GATCGGA 16550 0.0 26.11264 1 TCGTATG 8415 0.0 25.436802 42-43 >>END_MODULE