FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n01 kx48-1.3410000000cbc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n01 kx48-1.3410000000cbc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16111605
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC396780.24626969193944365TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT38550.036.7516441
AGTCACG71350.032.99390828-29
GAGCACA142450.031.5624649
ATCGGAA140650.030.5502782
TATGCCG79750.029.69588746-47
ACATGCG51700.029.6621134
ACGGCTA80400.029.4595132-33
CACACGT81900.029.27612-13
CGTATGC81400.029.20948844-45
AGAGCAC153750.028.347348
TCGGAAG155850.027.9057463
GCGTATT51400.027.8965262
ACGTCTG76600.027.83098614-15
ACACGTC79100.027.28108812-13
CTCGTAT85750.027.23398842-43
TAAGTCG52750.027.0011449
CATGCGT57500.026.834755
CACGTCT89500.026.76388514-15
GATCGGA165500.026.112641
TCGTATG84150.025.43680242-43
Produced by FastQC (version 0.11.2)