##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l06n01 kx24-1.3410000000c9e5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15056799 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.81198480500404 33.0 33.0 33.0 33.0 33.0 2 32.81584950426714 33.0 33.0 33.0 33.0 33.0 3 32.73483879276066 33.0 33.0 33.0 33.0 33.0 4 36.73147141035754 37.0 37.0 37.0 37.0 37.0 5 36.74706981211611 37.0 37.0 37.0 37.0 37.0 6 36.6946461860851 37.0 37.0 37.0 37.0 37.0 7 36.71692555635497 37.0 37.0 37.0 37.0 37.0 8 36.71334079707114 37.0 37.0 37.0 37.0 37.0 9 36.71384010638649 37.0 37.0 37.0 37.0 37.0 10-11 36.69573373464041 37.0 37.0 37.0 37.0 37.0 12-13 36.69348152950703 37.0 37.0 37.0 37.0 37.0 14-15 39.12176153776112 40.0 40.0 40.0 37.0 40.0 16-17 39.106455263167156 40.0 40.0 40.0 37.0 40.0 18-19 39.07659456701255 40.0 40.0 40.0 37.0 40.0 20-21 39.07023498155219 40.0 40.0 40.0 37.0 40.0 22-23 39.03868425154643 40.0 40.0 40.0 37.0 40.0 24-25 39.01222188726834 40.0 40.0 40.0 37.0 40.0 26-27 38.956903223586906 40.0 40.0 40.0 37.0 40.0 28-29 38.91570186996585 40.0 40.0 40.0 37.0 40.0 30-31 38.864466942807695 40.0 40.0 40.0 37.0 40.0 32-33 38.796322877126805 40.0 40.0 40.0 37.0 40.0 34-35 38.72317223601112 40.0 38.5 40.0 37.0 40.0 36-37 38.649645452529455 40.0 37.0 40.0 37.0 40.0 38-39 38.56047620081798 40.0 37.0 40.0 37.0 40.0 40-41 38.458433097234014 40.0 37.0 40.0 37.0 40.0 42-43 38.34876486695479 40.0 37.0 40.0 37.0 40.0 44-45 38.241560905475325 40.0 37.0 40.0 37.0 40.0 46-47 38.12657371596712 40.0 37.0 40.0 37.0 40.0 48-49 38.00103830170012 40.0 37.0 40.0 37.0 40.0 50-51 37.86812163727496 40.0 37.0 40.0 33.0 40.0 52-53 37.70002498539032 40.0 37.0 40.0 33.0 40.0 54-55 37.53358735146826 40.0 37.0 40.0 33.0 40.0 56-57 37.36772689865887 37.0 37.0 40.0 33.0 40.0 58-59 37.203544923459496 37.0 37.0 40.0 33.0 40.0 60-61 37.026828212291335 37.0 37.0 40.0 33.0 40.0 62-63 36.827648094392444 37.0 37.0 40.0 33.0 40.0 64-65 36.63843486919099 37.0 37.0 40.0 33.0 40.0 66-67 36.411454685687175 37.0 37.0 40.0 33.0 40.0 68-69 36.19540876516981 37.0 37.0 37.0 33.0 40.0 70-71 35.97842350156896 37.0 37.0 37.0 33.0 40.0 72-73 35.73871172750596 37.0 37.0 37.0 33.0 40.0 74-75 35.511137925132694 37.0 37.0 37.0 33.0 38.5 76-77 32.927668158418 33.0 33.0 37.0 27.0 37.0 78-79 35.008128985450355 37.0 33.0 37.0 33.0 37.0 80-81 35.21050134892549 37.0 35.0 37.0 33.0 37.0 82-83 35.16619714455908 37.0 35.0 37.0 33.0 37.0 84-85 35.07941734494828 37.0 33.0 37.0 33.0 37.0 86-87 35.01796660100198 37.0 33.0 37.0 33.0 37.0 88-89 34.94412969184221 37.0 33.0 37.0 33.0 37.0 90-91 34.86932607654522 37.0 33.0 37.0 33.0 37.0 92-93 34.78955792662172 37.0 33.0 37.0 33.0 37.0 94-95 34.72551028276329 37.0 33.0 37.0 33.0 37.0 96-97 34.65553133172595 37.0 33.0 37.0 33.0 37.0 98-99 34.60797510812225 37.0 33.0 37.0 33.0 37.0 100-101 32.858638612363755 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.058787850167661304 2306 2 0.07023980689898224 2306 3 0.15111633742276354 2306 4 0.13455403633722085 2306 5 0.13147814028469895 2306 6 0.1453504156295864 2306 7 0.12095858640593349 2306 8 0.14244595505429913 2306 9 0.12082511485425584 2306 10-11 0.15606472798575055 2306 12-13 0.14664004490155946 2306 14-15 0.31614025585270156 2306 16-17 0.33908264369316043 2306 18-19 0.3748275119546278 2306 20-21 0.3512777040914514 2306 22-23 0.33013793025622107 2306 24-25 0.363922120296138 2306 26-27 0.3612996051073907 2306 28-29 0.3992666175839972 2306 30-31 0.38648181956737204 2306 32-33 0.37130897654668615 2306 34-35 0.3890447495946532 2306 36-37 0.39858367804941963 2306 38-39 0.405411973171546 2306 40-41 0.3919898320748345 2306 42-43 0.3899043650360525 2306 44-45 0.4303067529979856 2306 46-47 0.4172282186268603 2306 48-49 0.38101100975443813 2306 50-51 0.41675413128093197 2306 52-53 0.41119957057146905 2306 54-55 0.4389590083210564 2306 56-57 0.3557334243055408 2306 58-59 0.4147605455777992 2306 60-61 0.38807672321553355 2306 62-63 0.42430208510707956 2306 64-65 0.41962238895599313 2306 66-67 0.38678507757089875 2306 68-69 0.36741927854895806 2306 70-71 0.3365957703990645 2306 72-73 0.31683227269763137 2306 74-75 0.3805894243994956 2306 76-77 0.04946703349923354 2306 78-79 0.34142938678332513 2306 80-81 0.4118199863431258 2306 82-83 0.4403259878511463 2306 84-85 0.484481416860028 2306 86-87 0.41685562711019486 2306 88-89 0.39896510126305174 2306 90-91 0.45648802123218246 2306 92-93 0.4488465258508185 2306 94-95 0.5005682934811091 2306 96-97 0.39033374988292735 2306 98-99 0.3822337125619626 2306 100-101 0.7329745801287046 2307 1 0.05192689054445765 2307 2 0.05183680022463477 2307 3 0.13396790590528695 2307 4 0.12193786786046701 2307 5 0.11489681735686474 2307 6 0.13451753176588 2307 7 0.12921650872426227 2307 8 0.1491855023884483 2307 9 0.13775180802298337 2307 10-11 0.14342553112692968 2307 12-13 0.1409191844058384 2307 14-15 0.32500154952617066 2307 16-17 0.33029578995834896 2307 18-19 0.3834992237791113 2307 20-21 0.3651990546755428 2307 22-23 0.325657189752242 2307 24-25 0.36322103091494284 2307 26-27 0.3656665632079452 2307 28-29 0.3634693306048504 2307 30-31 0.34467864731063 2307 32-33 0.3770708996995893 2307 34-35 0.3686932366022049 2307 36-37 0.35144699378130184 2307 38-39 0.3619249822652577 2307 40-41 0.37838471944107965 2307 42-43 0.3666195078342156 2307 44-45 0.38930649441422815 2307 46-47 0.4052259923649615 2307 48-49 0.35854427050674786 2307 50-51 0.39568063689319644 2307 52-53 0.409693292424123 2307 54-55 0.4248770694533661 2307 56-57 0.3949697920582551 2307 58-59 0.38925902625948083 2307 60-61 0.34643868942873723 2307 62-63 0.3802499277638489 2307 64-65 0.3678533930378478 2307 66-67 0.31078425853735325 2307 68-69 0.3508682254582709 2307 70-71 0.4051667177922553 2307 72-73 0.4610950148840729 2307 74-75 0.4414089351545769 2307 76-77 0.23593314237964336 2307 78-79 0.39642654569136226 2307 80-81 0.3855265126420946 2307 82-83 0.41298941167769954 2307 84-85 0.4231685160829457 2307 86-87 0.4235710640057704 2307 88-89 0.4902634247968436 2307 90-91 0.49597473246848267 2307 92-93 0.4335743248258055 2307 94-95 0.3980989295527593 2307 96-97 0.46977819987638725 2307 98-99 0.440539966737596 2307 100-101 0.5519679179900265 2201 1 0.03823746643310244 2201 2 0.06028411866587646 2201 3 0.12131182110960737 2201 4 0.1271874285524035 2201 5 0.12110573972853445 2201 6 0.14605220263319296 2201 7 0.1162111029058579 2201 8 0.09667511139350893 2201 9 0.1126121689367352 2201 10-11 0.11626631403111531 2201 12-13 0.1093801248095474 2201 14-15 0.22485155929671663 2201 16-17 0.20585834063760444 2201 18-19 0.2383156854933688 2201 20-21 0.2453490203984714 2201 22-23 0.2633048587329583 2201 24-25 0.2470869929205861 2201 26-27 0.2611659379022484 2201 28-29 0.2882327636380495 2201 30-31 0.26177402926472126 2201 32-33 0.26700940069372336 2201 34-35 0.24182841041666592 2201 36-37 0.24827468502585504 2201 38-39 0.2870616931655121 2201 40-41 0.26092191828224287 2201 42-43 0.2878428762288223 2201 44-45 0.27268704403095967 2201 46-47 0.30052016500687273 2201 48-49 0.25340584002726274 2201 50-51 0.24986799668496218 2201 52-53 0.30426401875442366 2201 54-55 0.30292856677445457 2201 56-57 0.31805378960999064 2201 58-59 0.2939897334646062 2201 60-61 0.33962256640837296 2201 62-63 0.2824183691608795 2201 64-65 0.35444886871221826 2201 66-67 0.3581574867500734 2201 68-69 0.29059445987933685 2201 70-71 0.2170739805730051 2201 72-73 0.2680632970757273 2201 74-75 0.3060035340450824 2201 76-77 0.0030760795858668644 2201 78-79 0.2644173593638257 2201 80-81 0.3513421799785519 2201 82-83 0.3503666443021416 2201 84-85 0.386527478888091 2201 86-87 0.35447119387659853 2201 88-89 0.29704775346605317 2201 90-91 0.26723174236481384 2201 92-93 0.307597190190819 2201 94-95 0.39418687535734165 2201 96-97 0.3089004149161383 2201 98-99 0.2771366184960513 2201 100-101 0.2513310907868487 1101 1 0.045308000390797076 1101 2 0.05762771356914698 1101 3 0.13496698739011492 1101 4 0.11761178529756933 1101 5 0.09905297362509913 1101 6 0.14072091549166288 1101 7 0.1405867254328257 1101 8 0.13458993045502154 1101 9 0.1298329839119745 1101 10-11 0.13739306695203624 1101 12-13 0.150306578819297 1101 14-15 0.27537734915420486 1101 16-17 0.2870498113378446 1101 18-19 0.3295121195228532 1101 20-21 0.3186996929364625 1101 22-23 0.2634160806049337 1101 24-25 0.3298351628677523 1101 26-27 0.2906266972007785 1101 28-29 0.3390544372609199 1101 30-31 0.36413863236439425 1101 32-33 0.29648583031530507 1101 34-35 0.3211656609066509 1101 36-37 0.3326837875956272 1101 38-39 0.2984981241331539 1101 40-41 0.2890751505034146 1101 42-43 0.3208348141890909 1101 44-45 0.3347384500439148 1101 46-47 0.3473395305361606 1101 48-49 0.35352740711907416 1101 50-51 0.34406354343736467 1101 52-53 0.3042144303679635 1101 54-55 0.3158091560033043 1101 56-57 0.3277257655910475 1101 58-59 0.36982193497183147 1101 60-61 0.3555294645566178 1101 62-63 0.30398007411709926 1101 64-65 0.3357927223114672 1101 66-67 0.32284732329756594 1101 68-69 0.3920497726039258 1101 70-71 0.404492980790117 1101 72-73 0.4208931604865427 1101 74-75 0.4142557067213417 1101 76-77 0.36152846051931675 1101 78-79 0.49533621743005796 1101 80-81 0.47708975378920826 1101 82-83 0.49847711628132174 1101 84-85 0.5431836422595566 1101 86-87 0.4924697420469002 1101 88-89 0.5378596139116425 1101 90-91 0.5226304750445152 1101 92-93 0.5302523665471099 1101 94-95 0.5224086855898804 1101 96-97 0.51587683431692 1101 98-99 0.5355111455224275 1101 100-101 0.5395526903353982 2304 1 0.04798283657518709 2304 2 0.05956988260579266 2304 3 0.12884005348961836 2304 4 0.11340855092635849 2304 5 0.1117434483167088 2304 6 0.13057284095452815 2304 7 0.11412826424678002 2304 8 0.13295858427468943 2304 9 0.12056007416038028 2304 10-11 0.12356906805322154 2304 12-13 0.11600378432709135 2304 14-15 0.2536704149355984 2304 16-17 0.2543367694724168 2304 18-19 0.26621074450705606 2304 20-21 0.27598453023644964 2304 22-23 0.2767046403096387 2304 24-25 0.2930647334929546 2304 26-27 0.29150916896193735 2304 28-29 0.2772545597988554 2304 30-31 0.31314595766087905 2304 32-33 0.3147023621000642 2304 34-35 0.2494915927490453 2304 36-37 0.28563709805600723 2304 38-39 0.286845486178386 2304 40-41 0.31577077019775146 2304 42-43 0.3307178540827067 2304 44-45 0.3190187345917579 2304 46-47 0.3427683878519048 2304 48-49 0.314940876102078 2304 50-51 0.35048240796920993 2304 52-53 0.3572780961348414 2304 54-55 0.3512259646597258 2304 56-57 0.3183485467881795 2304 58-59 0.3573273550475804 2304 60-61 0.3702806921842665 2304 62-63 0.3741284197509245 2304 64-65 0.36526285592888286 2304 66-67 0.32004935551567115 2304 68-69 0.3501326728576615 2304 70-71 0.3452920082832165 2304 72-73 0.3717430235334831 2304 74-75 0.3814213681314982 2304 76-77 0.23575196080832228 2304 78-79 0.39517410476665304 2304 80-81 0.38173220753869685 2304 82-83 0.36492903249828856 2304 84-85 0.43222522923543494 2304 86-87 0.4088026507178242 2304 88-89 0.42691956355417204 2304 90-91 0.4354950229849237 2304 92-93 0.42881174683084566 2304 94-95 0.48909269978159386 2304 96-97 0.44966986576064016 2304 98-99 0.41073730408266584 2304 100-101 0.4293780468359145 2202 1 0.030409455762374193 2202 2 0.035052044745533806 2202 3 0.11531047625488355 2202 4 0.09802748792704108 2202 5 0.09444734800312915 2202 6 0.09778980160247386 2202 7 0.09467662888813777 2202 8 0.09722767339723504 2202 9 0.10813342904701528 2202 10-11 0.10411762714092987 2202 12-13 0.11076460559070256 2202 14-15 0.24366098104670897 2202 16-17 0.23125470727809727 2202 18-19 0.26938806272887916 2202 20-21 0.2631191331316316 2202 22-23 0.2619909820089177 2202 24-25 0.2637170426730293 2202 26-27 0.28652484457875715 2202 28-29 0.2759581373524824 2202 30-31 0.2654243503932534 2202 32-33 0.2593985623590669 2202 34-35 0.283814825458542 2202 36-37 0.27657474753663536 2202 38-39 0.2797898220209376 2202 40-41 0.28240060008312184 2202 42-43 0.28132253362863935 2202 44-45 0.31347309219933805 2202 46-47 0.3042932069843829 2202 48-49 0.293574608525752 2202 50-51 0.2989236449850594 2202 52-53 0.3209366561169986 2202 54-55 0.28660669385947557 2202 56-57 0.28268437962135096 2202 58-59 0.326792326185263 2202 60-61 0.35533655013990995 2202 62-63 0.31194484878253803 2202 64-65 0.32302667749971903 2202 66-67 0.29603719159999287 2202 68-69 0.37546830346662574 2202 70-71 0.3513444588906083 2202 72-73 0.3673422488592095 2202 74-75 0.37723138950763513 2202 76-77 0.36206295052729587 2202 78-79 0.42481711098505315 2202 80-81 0.3725691176046695 2202 82-83 0.3731595577238096 2202 84-85 0.45815933331090974 2202 86-87 0.49347677807499934 2202 88-89 0.36714367593898345 2202 90-91 0.43116345500842357 2202 92-93 0.4435110485091798 2202 94-95 0.463165692998011 2202 96-97 0.47421178256094265 2202 98-99 0.5221890599980483 2202 100-101 0.5628351668780169 1102 1 0.05597919932145601 1102 2 0.05517598537937829 1102 3 0.11443354449037457 1102 4 0.11080268370665891 1102 5 0.09236316878718043 1102 6 0.08834823571685035 1102 7 0.07267560093710301 1102 8 0.08680102555177882 1102 9 0.09916522214438572 1102 10-11 0.09168127552840843 1102 12-13 0.11139618063099022 1102 14-15 0.2366311616833201 1102 16-17 0.2195292299877991 1102 18-19 0.2550814522500673 1102 20-21 0.24258559877927866 1102 22-23 0.22899774897542358 1102 24-25 0.2569384380513853 1102 26-27 0.27349616026824464 1102 28-29 0.21789052803848108 1102 30-31 0.24855997504491967 1102 32-33 0.21948887358058755 1102 34-35 0.22952216655327362 1102 36-37 0.22256990358656736 1102 38-39 0.21711876011748643 1102 40-41 0.22207827028368143 1102 42-43 0.2259201097835728 1102 44-45 0.24864669777158355 1102 46-47 0.2617788750293002 1102 48-49 0.2223748539533048 1102 50-51 0.25598584990601836 1102 52-53 0.20318991599395986 1102 54-55 0.23574708822659574 1102 56-57 0.22846425529821346 1102 58-59 0.2502420765784663 1102 60-61 0.2548796176897241 1102 62-63 0.27062192567288434 1102 64-65 0.28557659106740374 1102 66-67 0.3082388116712167 1102 68-69 0.20398883032836324 1102 70-71 0.3050202055080433 1102 72-73 0.2986061625362524 1102 74-75 0.3035474954999202 1102 76-77 0.18708734594730458 1102 78-79 0.34483918444841066 1102 80-81 0.34139004247159477 1102 82-83 0.3513144791243761 1102 84-85 0.38736358361074963 1102 86-87 0.3442233742622349 1102 88-89 0.3381993671977952 1102 90-91 0.36986115042041234 1102 92-93 0.36059574056729105 1102 94-95 0.39091875581324587 1102 96-97 0.3952614245489414 1102 98-99 0.4498310754847452 1102 100-101 0.3977054801452482 2305 1 0.04933740834056266 2305 2 0.04945166721370953 2305 3 0.10689080171061249 2305 4 0.08115114019004466 2305 5 0.06177840184906813 2305 6 0.09109483328629153 2305 7 0.08002086193064173 2305 8 0.05643120302858051 2305 9 0.0646098472071941 2305 10-11 0.03781796853454722 2305 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-0.6824656148672474 1213 30-31 -0.7299664671046955 1213 32-33 -0.7029828034162335 1213 34-35 -0.6175609803359166 1213 36-37 -0.7276199972471247 1213 38-39 -0.7114233977812532 1213 40-41 -0.635204345028022 1213 42-43 -0.6339675953971238 1213 44-45 -0.641724320535566 1213 46-47 -0.7786633737776043 1213 48-49 -0.6583166131071962 1213 50-51 -0.6412039640629672 1213 52-53 -0.6365043586049026 1213 54-55 -0.7181569144233819 1213 56-57 -1.1184541076564969 1213 58-59 -1.0388544407606872 1213 60-61 -0.6804190892252535 1213 62-63 -0.6840725170434823 1213 64-65 -0.6362419698055035 1213 66-67 -0.5822371901424361 1213 68-69 -0.6314636669312677 1213 70-71 -0.5312416060657554 1213 72-73 -0.5099335760052952 1213 74-75 -0.5159685894122177 1213 76-77 -0.30212647712007623 1213 78-79 -0.6526610929094971 1213 80-81 -0.6291150242446761 1213 82-83 -0.6393261963041255 1213 84-85 -0.6018856856297035 1213 86-87 -0.5790584564371599 1213 88-89 -0.6338438068165786 1213 90-91 -0.6362089947261538 1213 92-93 -0.6792146286422494 1213 94-95 -0.6672198629937682 1213 96-97 -0.7267153562411295 1213 98-99 -0.6590902418293396 1213 100-101 -0.302928325248935 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 29.0 8 323.0 9 1009.0 10 1194.0 11 838.0 12 679.0 13 676.0 14 865.0 15 1425.0 16 2602.0 17 4139.0 18 5837.0 19 8379.0 20 11635.0 21 15988.0 22 21505.0 23 27518.0 24 35526.0 25 45707.0 26 58177.0 27 74418.0 28 94779.0 29 120470.0 30 152805.0 31 194501.0 32 254487.0 33 343794.0 34 490594.0 35 803440.0 36 1843767.0 37 5173377.0 38 5263418.0 39 2897.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.557968802435038 12.69858863789038 11.66345056794989 55.0799919917247 2 22.415168935542994 19.661919705083157 39.08238574419832 18.840525615175522 3 23.208821745925164 24.868026484365817 24.636377946166522 27.286773823542497 4 26.148439065448358 29.51671191927999 18.401757529544042 25.933091485727612 5 28.024916095899165 30.805545119130105 21.55244686228583 19.617091922684892 6 20.298789935364084 33.86166608188101 22.667387669849347 23.17215631290555 7 19.01374920393106 14.68246338414958 41.634201266816405 24.669586145102954 8 20.95714451278213 20.197379716027978 27.56181689758723 31.283658873602665 9 21.605482015134825 19.478117493631945 31.361579576110433 27.554820915122797 10-11 25.018343873644316 28.352439210514746 21.207839143488545 25.421377772352393 12-13 22.90978860725542 22.562636132568677 27.802054947389333 26.72552031278657 14-15 23.66151553905858 24.1824307846146 26.264627309127697 25.891426367199124 16-17 25.048157943709597 24.464537595407656 24.828757365654877 25.658547095227867 18-19 24.522613716138537 24.850493837573897 24.858779257161494 25.768113189126073 20-21 24.552857342862815 24.932907016377275 25.203912327229922 25.310323313529988 22-23 24.451142571727992 25.00597903442695 25.17180135273288 25.371077041112176 24-25 24.230937133450475 25.09288992965902 25.249118355103235 25.427054581787274 26-27 24.365892777076986 25.123437591217098 25.365972541706906 25.144697089999006 28-29 24.59966703369957 24.77473435719552 25.092700769533778 25.532897839571135 30-31 24.359772121909117 24.81959451846876 25.193342833789668 25.627290525832453 32-33 24.57584031591651 25.056067347189547 25.18242172604347 25.18567061085047 34-35 24.792946489663713 24.956068734625052 24.853039961826724 25.39794481388451 36-37 24.59981888470334 25.012605875818245 24.843072336580356 25.54450290289806 38-39 24.701307695223505 25.108936951098208 25.14138091905994 25.048374434618342 40-41 24.83993439551324 24.923026501025593 24.98148469457903 25.255554408882137 42-43 24.405655058373213 24.793574764596592 25.282998654087628 25.517771522942567 44-45 24.702108833750376 24.962396247034146 25.18425755887766 25.15123736033782 46-47 24.918859016925335 24.835958413911303 24.917357651729315 25.32782491743405 48-49 24.661213002743416 24.795037557529827 25.002443696420727 25.541305743306026 50-51 24.720088255315943 24.999181209525926 25.114516068920835 25.166214466237296 52-53 25.02926445242611 24.749339846853243 24.87550230055081 25.34589340016984 54-55 24.55961525725838 24.810937086868538 25.07719873039494 25.552248925478143 56-57 24.722788481064004 24.98193227509915 25.18656635203953 25.108712891797314 58-59 24.93278546814545 24.85539265506521 24.827141985810407 25.38467989097893 60-61 24.627853657536406 24.84902778723485 25.02775559866539 25.495362956563355 62-63 24.848410295589332 24.93133559002048 25.124696136912732 25.095557977477455 64-65 24.872622302639527 24.92744352535313 24.870585734581628 25.329348437425715 66-67 24.72544779383124 24.859990530529476 25.008153602038856 25.406408073600424 68-69 24.871799463519125 25.082130506247385 25.038098767114626 25.00797126311886 70-71 25.0100467167343 24.890858690851992 24.854746344164955 25.244348248248755 72-73 24.793852245735096 24.92121039628285 24.94316789590889 25.341769462073167 74-75 24.887094291033694 25.11394083234753 24.993690399614447 25.005274477004324 76-77 24.997356038979692 24.977124422679818 24.832447274235623 25.193072264104867 78-79 24.81164348813765 24.954134540474744 24.943725782158904 25.2904961892287 80-81 24.96220623910204 25.15137761097737 24.864485739853798 25.02193041006679 82-83 25.1079718585745 25.03070820214186 24.700160823706426 25.16115911557721 84-85 24.798728258764683 25.045895778764 24.927331752811757 25.22804420965956 86-87 24.982365908762983 25.242733793048945 24.928881671022125 24.846018627165947 88-89 25.066503318366568 25.05086895732195 24.677643418281026 25.204984306030465 90-91 24.956050178328066 24.952709463875436 24.813060294018072 25.27818006377842 92-93 24.997408931970227 25.21582243454793 24.838939510142858 24.947829123338988 94-95 25.193856383076756 25.03014853752321 24.7108322304744 25.06516284892563 96-97 24.89241903275723 25.099468353134025 24.826017136842964 25.182095477265786 98-99 25.06190143940626 25.187203640933365 24.87010767926528 24.880787240395094 100-101 25.31601840683646 25.036559900488797 24.536533234790905 25.11088845788384 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.5 4 0.5 5 1.0 6 2.0 7 3.5 8 6.5 9 9.5 10 13.0 11 19.0 12 26.0 13 40.0 14 66.0 15 98.0 16 162.0 17 238.0 18 371.5 19 554.0 20 808.5 21 1226.0 22 1895.5 23 2966.5 24 4572.5 25 7015.5 26 10788.5 27 15871.5 28 22578.5 29 31673.5 30 42997.5 31 57199.0 32 74963.0 33 97523.0 34 126230.0 35 162146.0 36 206409.5 37 260785.5 38 323652.5 39 391580.5 40 460816.5 41 525860.0 42 583587.0 43 625467.0 44 646819.0 45 649071.0 46 633417.0 47 606842.5 48 574410.5 49 538766.0 50 502168.0 51 468793.0 52 443100.0 53 425416.5 54 416488.5 55 408496.0 56 399944.5 57 396312.0 58 386650.0 59 368825.0 60 351364.5 61 332323.5 62 318151.0 63 300662.0 64 265795.5 65 237391.5 66 223708.5 67 204510.0 68 180767.5 69 155778.0 70 129436.5 71 106201.5 72 88063.0 73 72521.0 74 54880.5 75 40064.5 76 29279.5 77 20523.5 78 13931.5 79 9343.5 80 6289.5 81 4058.0 82 2477.0 83 1388.0 84 744.5 85 458.0 86 324.0 87 210.0 88 132.5 89 92.0 90 61.5 91 43.5 92 27.0 93 17.5 94 11.5 95 9.5 96 7.0 97 2.5 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1152701845857144 2 5.313214316004352E-4 3 0.002450720103257007 4 0.0013947187579511423 5 1.7267946527014142E-4 6 0.0 7 0.0 8 2.2581160843018495E-4 9 0.0 10-11 6.641517895005439E-6 12-13 9.630200947757888E-5 14-15 2.058870547451686E-4 16-17 6.176611642355059E-4 18-19 0.0015209075979562457 20-21 5.512459852854514E-4 22-23 1.328303579001088E-4 24-25 0.0 26-27 0.0 28-29 1.328303579001088E-4 30-31 0.023158972899883966 32-33 0.03639883882357731 34-35 0.056954336708619144 36-37 0.04828051433774204 38-39 0.041413184834306414 40-41 0.0560411279980559 42-43 0.06726861399956259 44-45 0.056014561926475866 46-47 0.025453617332608345 48-49 0.018333910149162514 50-51 0.02728335551268234 52-53 0.06331359009308685 54-55 0.06480461086051557 56-57 0.06602000863530157 58-59 0.05046225296625132 60-61 0.07130001536183089 62-63 0.06323057111939928 64-65 0.046294700487135416 66-67 0.013967112133196437 68-69 0.0493298741651529 70-71 0.08024946072535072 72-73 0.07853926986738682 74-75 0.06829804927328843 76-77 0.024088785405184727 78-79 0.0144087730732143 80-81 5.379629494954406E-4 82-83 0.003725891539098051 84-85 0.008105972590854138 86-87 1.7600022421764415E-4 88-89 0.001457813177953694 90-91 9.264917463532587E-4 92-93 0.0017699645190189493 94-95 6.541895126580357E-4 96-97 0.0 98-99 3.3207589475027194E-6 100-101 1.3283035790010878E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5056799E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.30070487256675 #Duplication Level Percentage of deduplicated Percentage of total 1 78.40870205347771 46.496912999143 2 11.894401750709443 14.10692815709124 3 3.7806719344523882 6.725895318148713 4 1.7982221662326012 4.265433679002686 5 1.132711220648891 3.358528690077236 6 0.8043003225092459 2.8617345632418627 7 0.4287418313319472 1.7797284964437716 8 0.2860293280206662 1.3569392612681692 9 0.2893139760387792 1.5440870437726044 >10 1.0874718662187808 11.056994956883196 >50 0.059945341337770255 2.430657212106484 >100 0.028224452854276334 3.1823596127610383 >500 8.582527420250158E-4 0.34183448911288405 >1k 3.821841478548736E-4 0.40735010108623554 >5k 2.3319277457722164E-5 0.08461541986082634 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.010726051400433784 5.977366105504895E-5 1.3283035790010878E-5 2 0.011894958549954742 6.64151789500544E-5 3.9849107370032635E-5 3 0.015554434910102739 6.64151789500544E-5 3.9849107370032635E-5 4 0.027044260868462146 6.64151789500544E-5 3.9849107370032635E-5 5 0.029103131415913835 6.64151789500544E-5 3.9849107370032635E-5 6 0.03543913948774902 8.633973263507071E-5 3.9849107370032635E-5 7 0.03684714128149018 1.0626428632008702E-4 3.9849107370032635E-5 8 0.0399288055847727 1.1290580421509246E-4 3.9849107370032635E-5 9 0.04404654667967607 1.1290580421509246E-4 4.649062526503807E-5 10-11 0.05318859606215106 1.2618884000510335E-4 4.649062526503807E-5 12-13 0.06262287223200629 1.3947187579511423E-4 4.649062526503807E-5 14-15 0.07265488501241199 1.527549115851251E-4 4.981138421254079E-5 16-17 0.08273338841808275 1.7267946527014142E-4 5.313214316004351E-5 18-19 0.09263257084058836 1.859625010601523E-4 5.977366105504895E-5 20-21 0.1009876003525052 1.9260401895515773E-4 5.977366105504895E-5 22-23 0.10881462919176912 1.9924553685016317E-4 5.977366105504895E-5 24-25 0.11600739307206001 2.1917009053517948E-4 6.973593789755711E-5 26-27 0.12246294846600529 2.424154031676985E-4 8.633973263507071E-5 28-29 0.1279388799704373 2.6233995685271484E-4 8.633973263507071E-5 30-31 0.1328502824537938 2.9222678738023933E-4 1.0958504526758974E-4 32-33 0.1369979103792247 3.3207589475027196E-4 1.1290580421509246E-4 34-35 0.1405278771404201 3.6196272527779644E-4 1.2950959895260608E-4 36-37 0.14389180595424034 3.719250021203046E-4 1.328303579001088E-4 38-39 0.14746494258175327 3.719250021203046E-4 1.361511168476115E-4 40-41 0.15073589014504346 3.719250021203046E-4 1.693587063226387E-4 42-43 0.1532397423914605 3.8188727896281273E-4 1.9260401895515773E-4 44-45 0.15571702856629754 4.051325915953318E-4 2.2581160843018492E-4 46-47 0.15841348483166973 4.051325915953318E-4 2.2581160843018492E-4 48-49 0.16110994109704194 4.1509486843783994E-4 2.4573616211520123E-4 50-51 0.16389937861294424 4.3501942212285623E-4 2.523776800102067E-4 52-53 0.166658929298319 4.3834018107035896E-4 2.7894375159022845E-4 54-55 0.16925244203631862 4.4498169896536443E-4 2.95547546327742E-4 56-57 0.1717762188364207 4.582647347553753E-4 2.9886830527524474E-4 58-59 0.17429667487757525 4.649062526503807E-4 3.1215134106525567E-4 60-61 0.1768038478829398 4.947930831779052E-4 3.2211361790776376E-4 62-63 0.17964309678305462 5.512459852854514E-4 3.353966536977747E-4 64-65 0.182545440103172 5.744912979179705E-4 3.420381715927801E-4 66-67 0.1855075570843444 5.844535747604787E-4 3.4867968948778556E-4 68-69 0.18845639102972683 5.944158516029867E-4 3.586419663302937E-4 70-71 0.19175722542354454 6.076988873929977E-4 3.6196272527779644E-4 72-73 0.1953768526763225 6.342649589730194E-4 3.918495558053209E-4 74-75 0.1995012352891209 6.50868753710533E-4 3.9849107370032634E-4 76-77 0.20399422214509205 6.741140663430521E-4 4.051325915953318E-4 78-79 0.20927090811267388 6.774348252905547E-4 4.117741094903372E-4 80-81 0.21498261350237857 6.873971021330629E-4 4.2505714528034814E-4 82-83 0.22190639590792172 6.973593789755711E-4 4.6822701159788345E-4 84-85 0.23050384082300626 7.040008968705766E-4 4.981138421254079E-4 86-87 0.24134279802765513 7.206046916080901E-4 5.014346010729107E-4 88-89 0.2543136824766008 7.538122810831174E-4 5.280006726529324E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAATTA 4940 0.0 12.206877 14-15 CTCGGAT 7705 0.0 12.153656 1 CTCGAAT 7025 0.0 11.9091215 1 CTCGATT 7730 0.0 11.806878 1 CGGAAAT 4770 0.0 11.4602165 1 CGCAAAT 3600 0.0 11.355579 1 CTCGACT 7795 0.0 11.281556 1 CGCGTAT 1060 0.0 11.211082 1 CTCGGTT 6595 0.0 10.95575 1 CTCGTTT 6530 0.0 10.773626 1 CGACAAT 5235 0.0 10.260655 1 CTCGGGT 7600 0.0 10.257549 1 CTGGGAT 10005 0.0 10.214914 1 CCGGGAT 5460 0.0 10.099008 1 CTCAGAT 11575 0.0 9.897133 1 CGCGAAT 1830 0.0 9.870653 1 TTAGGTG 6610 0.0 9.589501 18-19 CTGGAAT 14175 0.0 9.557299 1 CGAGAAT 6035 0.0 9.530626 1 CTTTGAT 18920 0.0 9.421576 1 >>END_MODULE