FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n01 kx24-1.3410000000c9e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n01 kx24-1.3410000000c9e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15056799
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA49400.012.20687714-15
CTCGGAT77050.012.1536561
CTCGAAT70250.011.90912151
CTCGATT77300.011.8068781
CGGAAAT47700.011.46021651
CGCAAAT36000.011.3555791
CTCGACT77950.011.2815561
CGCGTAT10600.011.2110821
CTCGGTT65950.010.955751
CTCGTTT65300.010.7736261
CGACAAT52350.010.2606551
CTCGGGT76000.010.2575491
CTGGGAT100050.010.2149141
CCGGGAT54600.010.0990081
CTCAGAT115750.09.8971331
CGCGAAT18300.09.8706531
TTAGGTG66100.09.58950118-19
CTGGAAT141750.09.5572991
CGAGAAT60350.09.5306261
CTTTGAT189200.09.4215761
Produced by FastQC (version 0.11.2)