FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n01 kx12-2.3410000000cd0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n01 kx12-2.3410000000cd0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17067317
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1066520.6248902507640773TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA250050.045.4598162
AGAGCAC265400.043.1693348
GAGCACA269450.042.8551569
TCGGAAG277550.040.989223
GATCGGA280900.040.544921
CGGAAGA293300.038.965634
AGTCACG173250.035.0999428-29
TATGCCG184200.033.19752548-49
CACACGT188900.032.67735712-13
TCACGTG187750.032.6181430-31
CGTATGC190150.032.21600746-47
CGTCTGA193050.031.86453416-17
CTCGTAT187800.030.92363444-45
ACGTCTG185100.030.83448614-15
GTCACGT186250.030.48184828-29
CACGTGA186400.030.45847930-31
TGAAACG187150.029.98338534-35
AAGAGCA383900.029.9305677
CACGTCT208650.029.48205214-15
ACACGTC194550.029.33652912-13
Produced by FastQC (version 0.11.2)