##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	C5B16ACXX l06n01 kx12-2.3410000000cd0d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17067317
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81850732601967	33.0	33.0	33.0	33.0	33.0
2	32.82023864676563	33.0	33.0	33.0	33.0	33.0
3	32.737425571927915	33.0	33.0	33.0	33.0	33.0
4	36.738813136241625	37.0	37.0	37.0	37.0	37.0
5	36.75398558543208	37.0	37.0	37.0	37.0	37.0
6	36.702213593384364	37.0	37.0	37.0	37.0	37.0
7	36.71584420679595	37.0	37.0	37.0	37.0	37.0
8	36.71644096140008	37.0	37.0	37.0	37.0	37.0
9	36.717363074700025	37.0	37.0	37.0	37.0	37.0
10-11	36.696396363880744	37.0	37.0	37.0	37.0	37.0
12-13	36.69393716071484	37.0	37.0	37.0	37.0	37.0
14-15	39.12835875140773	40.0	40.0	40.0	37.0	40.0
16-17	39.11478611430256	40.0	40.0	40.0	37.0	40.0
18-19	39.08112678167283	40.0	40.0	40.0	37.0	40.0
20-21	39.080132425032005	40.0	40.0	40.0	37.0	40.0
22-23	39.04819629822309	40.0	40.0	40.0	37.0	40.0
24-25	39.02392602188147	40.0	40.0	40.0	37.0	40.0
26-27	38.96970786328045	40.0	40.0	40.0	37.0	40.0
28-29	38.92846221816821	40.0	40.0	40.0	37.0	40.0
30-31	38.87233336089088	40.0	40.0	40.0	37.0	40.0
32-33	38.81094163775127	40.0	40.0	40.0	37.0	40.0
34-35	38.735551873794805	40.0	38.5	40.0	37.0	40.0
36-37	38.66543540499072	40.0	37.0	40.0	37.0	40.0
38-39	38.57865814527263	40.0	37.0	40.0	37.0	40.0
40-41	38.48219948689065	40.0	37.0	40.0	37.0	40.0
42-43	38.343308998127824	40.0	37.0	40.0	37.0	40.0
44-45	38.20821178278929	40.0	37.0	40.0	37.0	40.0
46-47	38.138292122891954	40.0	37.0	40.0	37.0	40.0
48-49	38.00835383206393	40.0	37.0	40.0	37.0	40.0
50-51	37.88936512399694	40.0	37.0	40.0	33.0	40.0
52-53	37.730957127004785	40.0	37.0	40.0	33.0	40.0
54-55	37.54980466466991	40.0	37.0	40.0	33.0	40.0
56-57	37.3869845506473	37.0	37.0	40.0	33.0	40.0
58-59	37.218456245934846	37.0	37.0	40.0	33.0	40.0
60-61	37.0396605394978	37.0	37.0	40.0	33.0	40.0
62-63	36.843951161157904	37.0	37.0	40.0	33.0	40.0
64-65	36.658915897560234	37.0	37.0	40.0	33.0	40.0
66-67	36.41209054709654	37.0	37.0	40.0	33.0	40.0
68-69	36.21186470023379	37.0	37.0	37.0	33.0	40.0
70-71	35.99429034452223	37.0	37.0	37.0	33.0	40.0
72-73	35.70347632847037	37.0	37.0	37.0	33.0	40.0
74-75	35.425413203492965	37.0	37.0	37.0	33.0	38.5
76-77	32.85523650260905	33.0	33.0	37.0	27.0	37.0
78-79	34.90687962261438	37.0	33.0	37.0	33.0	37.0
80-81	35.09428048942901	37.0	35.0	37.0	33.0	37.0
82-83	35.04520071901166	37.0	35.0	37.0	33.0	37.0
84-85	34.95447954707819	37.0	33.0	37.0	33.0	37.0
86-87	34.892701383585944	37.0	33.0	37.0	33.0	37.0
88-89	34.82188225601013	37.0	33.0	37.0	33.0	37.0
90-91	34.74876273757616	37.0	33.0	37.0	33.0	37.0
92-93	34.66956549175245	37.0	33.0	37.0	33.0	37.0
94-95	34.60943899383834	37.0	33.0	37.0	33.0	37.0
96-97	34.54260133564051	37.0	33.0	37.0	33.0	37.0
98-99	34.49747959799423	37.0	33.0	37.0	33.0	37.0
100-101	32.75855733505155	35.0	30.0	37.0	27.5	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2306	1	0.055660993333120246
2306	2	0.07775064746568461
2306	3	0.1484640710592231
2306	4	0.14387423468415506
2306	5	0.14027539956706647
2306	6	0.16389532577081667
2306	7	0.13058838069056833
2306	8	0.16047672760851128
2306	9	0.10810412389619728
2306	10-11	0.1572582892134946
2306	12-13	0.15566600296715905
2306	14-15	0.32115430704485703
2306	16-17	0.3357949604355568
2306	18-19	0.3721401796554247
2306	20-21	0.34252237956233245
2306	22-23	0.3525771170596599
2306	24-25	0.34438801528502694
2306	26-27	0.37945958195670215
2306	28-29	0.3775840606631675
2306	30-31	0.331709741615569
2306	32-33	0.34394099983507687
2306	34-35	0.3704317003667228
2306	36-37	0.340408930240109
2306	38-39	0.3626981167523482
2306	40-41	0.36258119669507494
2306	42-43	0.38035593201340134
2306	44-45	0.39357240896095647
2306	46-47	0.3830111626824291
2306	48-49	0.3858762667800377
2306	50-51	0.39937104066910933
2306	52-53	0.3829681945638228
2306	54-55	0.3616034810348907
2306	56-57	0.3375353291954468
2306	58-59	0.3598592918197596
2306	60-61	0.36325324212945276
2306	62-63	0.3741796915395952
2306	64-65	0.3816790130068739
2306	66-67	0.43536325685646915
2306	68-69	0.375417746028333
2306	70-71	0.3642985979267266
2306	72-73	0.3234417249802277
2306	74-75	0.33493596735626596
2306	76-77	0.024674787982881696
2306	78-79	0.3608779952705774
2306	80-81	0.37535861598926346
2306	82-83	0.37065880866548184
2306	84-85	0.4661694149077533
2306	86-87	0.4334953588331061
2306	88-89	0.3959070659348001
2306	90-91	0.41002805840832224
2306	92-93	0.37842631038269303
2306	94-95	0.40786729460267424
2306	96-97	0.3475052106404135
2306	98-99	0.3478470525556645
2306	100-101	0.6116029342591887
2307	1	0.04726090125209481
2307	2	0.056532656586092855
2307	3	0.13072213911149078
2307	4	0.13443264036686742
2307	5	0.12464913893389706
2307	6	0.12514872399935228
2307	7	0.1328936950815205
2307	8	0.15548494912916055
2307	9	0.15433428262635118
2307	10-11	0.11515171638178145
2307	12-13	0.15629083853674075
2307	14-15	0.349570513306368
2307	16-17	0.3559666257867846
2307	18-19	0.375679982777406
2307	20-21	0.3425305462728474
2307	22-23	0.33448445504089364
2307	24-25	0.3772360596593103
2307	26-27	0.39696314461564697
2307	28-29	0.3792086331441027
2307	30-31	0.4088446512884616
2307	32-33	0.397662608183758
2307	34-35	0.37884702966603356
2307	36-37	0.4164046112012656
2307	38-39	0.4067106025017111
2307	40-41	0.3991245953620819
2307	42-43	0.37326476083038074
2307	44-45	0.3877178641043386
2307	46-47	0.42596159239391085
2307	48-49	0.39268017803146194
2307	50-51	0.4522354184644257
2307	52-53	0.4739401975454882
2307	54-55	0.44844153900208994
2307	56-57	0.44043166130103373
2307	58-59	0.4287752569166585
2307	60-61	0.40286310356943034
2307	62-63	0.43001472398719187
2307	64-65	0.4550446295113062
2307	66-67	0.4425091285568996
2307	68-69	0.4677498077664737
2307	70-71	0.46754434466004824
2307	72-73	0.47562108567478845
2307	74-75	0.4880566921655216
2307	76-77	0.2762227907014676
2307	78-79	0.4896266798272748
2307	80-81	0.4553368563646032
2307	82-83	0.4881516833475814
2307	84-85	0.4948208662801932
2307	86-87	0.48158741794035365
2307	88-89	0.4591151032933922
2307	90-91	0.453238178552148
2307	92-93	0.4438307543886566
2307	94-95	0.4781427155445286
2307	96-97	0.47474787704399546
2307	98-99	0.5411920056381945
2307	100-101	0.6531310957079342
2201	1	0.029535770516723403
2201	2	0.049593286036262896
2201	3	0.11969396453176273
2201	4	0.12227138900042434
2201	5	0.11415627767588887
2201	6	0.1372133272138143
2201	7	0.12147041532260516
2201	8	0.11578453254866616
2201	9	0.12264979064774195
2201	10-11	0.13938390461115802
2201	12-13	0.10770556879348447
2201	14-15	0.24917346925519723
2201	16-17	0.23833984202456548
2201	18-19	0.256117558485748
2201	20-21	0.2518801378171531
2201	22-23	0.24229082451233808
2201	24-25	0.2822061917980463
2201	26-27	0.26361402024716796
2201	28-29	0.3012559157688486
2201	30-31	0.2813431555872228
2201	32-33	0.23808185693548722
2201	34-35	0.23685472791107287
2201	36-37	0.23951182897535972
2201	38-39	0.2827708238654978
2201	40-41	0.24925730653294664
2201	42-43	0.28859072214157777
2201	44-45	0.2908564362082231
2201	46-47	0.29203894046020906
2201	48-49	0.24514131749729273
2201	50-51	0.25181883796985005
2201	52-53	0.2909256578521635
2201	54-55	0.30807515682641906
2201	56-57	0.2669201702530799
2201	58-59	0.3109054678659362
2201	60-61	0.32725141715704353
2201	62-63	0.3229905416372105
2201	64-65	0.354915712989893
2201	66-67	0.3421767966663296
2201	68-69	0.2915017743366235
2201	70-71	0.23015251525123404
2201	72-73	0.23114321466701426
2201	74-75	0.23322704515101833
2201	76-77	-0.010095180610619536
2201	78-79	0.2747212598491373
2201	80-81	0.351513945545328
2201	82-83	0.3411366967904286
2201	84-85	0.40336649070776787
2201	86-87	0.3615430362373999
2201	88-89	0.34780299476087606
2201	90-91	0.2631978869457967
2201	92-93	0.34350328814349496
2201	94-95	0.43465101945625406
2201	96-97	0.30538495344662664
2201	98-99	0.35927143291387154
2201	100-101	0.3135962126311469
1101	1	0.024804355166637038
1101	2	0.06059141261629719
1101	3	0.12570128556264137
1101	4	0.11763180504397042
1101	5	0.08324213946335846
1101	6	0.11560187289506985
1101	7	0.1271876563110439
1101	8	0.13654622648037673
1101	9	0.11824593207416001
1101	10-11	0.13635692160077184
1101	12-13	0.1333586760463632
1101	14-15	0.26120299484915677
1101	16-17	0.27096150835149047
1101	18-19	0.3069369921875804
1101	20-21	0.3005653920707445
1101	22-23	0.27539791724174023
1101	24-25	0.32994817243183405
1101	26-27	0.2724895318834726
1101	28-29	0.31379413072610873
1101	30-31	0.35393975685524737
1101	32-33	0.30587594422014064
1101	34-35	0.32511146919861034
1101	36-37	0.34253135235941556
1101	38-39	0.3153857181852757
1101	40-41	0.3023843426001491
1101	42-43	0.30606930235738616
1101	44-45	0.31913758827457883
1101	46-47	0.31314688488486553
1101	48-49	0.34876468066595834
1101	50-51	0.3309335035594998
1101	52-53	0.331332155692742
1101	54-55	0.3176576660089623
1101	56-57	0.35651404807286013
1101	58-59	0.39968210581650965
1101	60-61	0.3736659504380455
1101	62-63	0.3360660223395797
1101	64-65	0.353676227312377
1101	66-67	0.31939214792245707
1101	68-69	0.34189391671273484
1101	70-71	0.4077477627131785
1101	72-73	0.4122309127251569
1101	74-75	0.3708443363148177
1101	76-77	0.31941351161032117
1101	78-79	0.4308095651553927
1101	80-81	0.3744277833486436
1101	82-83	0.3773981606822616
1101	84-85	0.4065174535413263
1101	86-87	0.3997273483038768
1101	88-89	0.42519916307290373
1101	90-91	0.4136568831718108
1101	92-93	0.41456352191735135
1101	94-95	0.4725697497742658
1101	96-97	0.44068557817396226
1101	98-99	0.43984572650570897
1101	100-101	0.5069826644970945
2304	1	0.045738397812698395
2304	2	0.0494564477040953
2304	3	0.12527527314216513
2304	4	0.122977377917735
2304	5	0.09170389257164402
2304	6	0.12533503340617358
2304	7	0.10883042849783209
2304	8	0.1105531910001929
2304	9	0.09969001385164944
2304	10-11	0.11712285098022335
2304	12-13	0.12377135775729897
2304	14-15	0.24746359558240982
2304	16-17	0.24828907863620486
2304	18-19	0.2740966621825578
2304	20-21	0.26391585625471947
2304	22-23	0.27695148966773075
2304	24-25	0.27663517633653356
2304	26-27	0.3004907398085095
2304	28-29	0.29186977494888566
2304	30-31	0.28014067360628303
2304	32-33	0.2764783296550135
2304	34-35	0.22743770764446936
2304	36-37	0.26306013169566
2304	38-39	0.27959750661369753
2304	40-41	0.2775513563663168
2304	42-43	0.28134858368539994
2304	44-45	0.3127723666120019
2304	46-47	0.35128630541409933
2304	48-49	0.30435295916978333
2304	50-51	0.331700939031613
2304	52-53	0.34514391135910216
2304	54-55	0.34075348260337535
2304	56-57	0.3395431794369941
2304	58-59	0.36512977616632725
2304	60-61	0.37850161091043333
2304	62-63	0.36855649529140067
2304	64-65	0.3622559391001019
2304	66-67	0.3247722928687651
2304	68-69	0.34520960875498474
2304	70-71	0.3528565375716326
2304	72-73	0.43594054862035847
2304	74-75	0.42499749097756734
2304	76-77	0.2408142493669132
2304	78-79	0.4031434291120988
2304	80-81	0.3973202744998332
2304	82-83	0.40778786318583826
2304	84-85	0.4469755351160458
2304	86-87	0.3927682115842188
2304	88-89	0.4595375477717951
2304	90-91	0.4351782403519806
2304	92-93	0.42879353072274284
2304	94-95	0.45011920030566444
2304	96-97	0.42231875760069926
2304	98-99	0.44470577957743274
2304	100-101	0.4502571393536172
2202	1	0.021410360601528566
2202	2	0.04087779115909029
2202	3	0.11857224571773628
2202	4	0.09128773677051072
2202	5	0.10143749353355247
2202	6	0.09106599789286918
2202	7	0.10918079270767578
2202	8	0.13907082291301265
2202	9	0.11131111690232842
2202	10-11	0.10364677688045276
2202	12-13	0.11552600879640096
2202	14-15	0.2589309657055381
2202	16-17	0.23368494814987173
2202	18-19	0.2649115017570054
2202	20-21	0.27166762851054926
2202	22-23	0.2824588849510761
2202	24-25	0.2763348655237863
2202	26-27	0.29767794368608946
2202	28-29	0.3146448840628082
2202	30-31	0.3046670646690117
2202	32-33	0.2564302390936959
2202	34-35	0.2961040622326223
2202	36-37	0.32410558463338646
2202	38-39	0.3148330055662214
2202	40-41	0.28370485867374384
2202	42-43	0.3178801628970973
2202	44-45	0.3337344588084008
2202	46-47	0.30877404544200004
2202	48-49	0.25886081066603595
2202	50-51	0.2898137037496795
2202	52-53	0.3283531354904241
2202	54-55	0.3382809768504984
2202	56-57	0.3693636383386334
2202	58-59	0.40444372582321364
2202	60-61	0.4038166484144625
2202	62-63	0.3760600580750477
2202	64-65	0.3954014447561036
2202	66-67	0.36351946300644755
2202	68-69	0.3600452429815064
2202	70-71	0.3796344606491857
2202	72-73	0.384224010893746
2202	74-75	0.4137115233483968
2202	76-77	0.440857009916094
2202	78-79	0.4793048963246349
2202	80-81	0.4143436482591909
2202	82-83	0.4166027701063655
2202	84-85	0.4377786899289333
2202	86-87	0.4749528751187171
2202	88-89	0.37391846709400767
2202	90-91	0.3887854018456878
2202	92-93	0.47763309386181874
2202	94-95	0.3853083034498468
2202	96-97	0.4325324960374175
2202	98-99	0.4815125392301809
2202	100-101	0.59163995658988
1102	1	0.026417121580855962
1102	2	0.03692779017376324
1102	3	0.1185928981324409
1102	4	0.11287107166210575
1102	5	0.0857928248545008
1102	6	0.08912873982800562
1102	7	0.09000000526960861
1102	8	0.10557685987408405
1102	9	0.09150830559825351
1102	10-11	0.10016397101473018
1102	12-13	0.09595755482592949
1102	14-15	0.22787852385720697
1102	16-17	0.20880104396410815
1102	18-19	0.21762960887241434
1102	20-21	0.23046026562359856
1102	22-23	0.23701549073198436
1102	24-25	0.23568857949201316
1102	26-27	0.26612365935413607
1102	28-29	0.24866803640473734
1102	30-31	0.2540201014731096
1102	32-33	0.2197337189791284
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1213	48-49	-0.6869156422177127
1213	50-51	-0.6911949858743895
1213	52-53	-0.6796124030426611
1213	54-55	-0.7024091384169822
1213	56-57	-1.1507591949128724
1213	58-59	-1.064831576758138
1213	60-61	-0.6993358579368021
1213	62-63	-0.6939873352183241
1213	64-65	-0.6590740731443532
1213	66-67	-0.6162958817279147
1213	68-69	-0.6489835006475744
1213	70-71	-0.5724042690060358
1213	72-73	-0.4876751093628542
1213	74-75	-0.5121144535484561
1213	76-77	-0.33576818248216256
1213	78-79	-0.6648978706621094
1213	80-81	-0.6239719251538531
1213	82-83	-0.6590647471466866
1213	84-85	-0.5977815836808844
1213	86-87	-0.5843747793856906
1213	88-89	-0.625785510165521
1213	90-91	-0.6170261534510004
1213	92-93	-0.6858101632363898
1213	94-95	-0.6659418831765151
1213	96-97	-0.7289604914913923
1213	98-99	-0.6668579626213358
1213	100-101	-0.3491415000350244
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	20.0
8	224.0
9	765.0
10	860.0
11	561.0
12	479.0
13	510.0
14	735.0
15	1442.0
16	2671.0
17	4542.0
18	6712.0
19	9612.0
20	13485.0
21	18614.0
22	25566.0
23	33216.0
24	43089.0
25	55530.0
26	71050.0
27	90098.0
28	115721.0
29	153523.0
30	205232.0
31	231681.0
32	282596.0
33	380025.0
34	543008.0
35	886950.0
36	2032654.0
37	5830016.0
38	6022303.0
39	3824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.54219762279009	13.121394349498239	9.752628354529344	56.58377967318232
2	20.76261791891786	19.75036848380136	42.10648402588445	17.38052957139633
3	22.85553422703737	23.281452905872094	25.966262243821703	27.896750623268833
4	26.38204847637923	28.84125135677906	17.99478235139882	26.781917815442892
5	28.280574694837213	30.11522512896604	22.065942067876765	19.53825810831999
6	20.739738999398675	32.4057612570271	23.928096021184817	22.926403722389406
7	18.49025245151303	15.278493977700187	41.92051978644329	24.31073378434349
8	20.347668511208454	20.17856728530806	28.57619123174315	30.897572971740335
9	22.311972057471014	19.134307987599925	31.446084935318186	27.107635019610875
10-11	25.13369486841677	28.438956905671546	21.413698598353093	25.013649627558593
12-13	22.572011599372544	22.842039625183887	28.015701368157035	26.570247407286534
14-15	23.50750261853467	24.361271039497257	26.21876152516326	25.912464816804814
16-17	25.068129206642702	24.323420187830425	24.832718635853805	25.77573196967306
18-19	24.351653315904052	24.49088053511251	25.108072184643348	26.04939396434009
20-21	24.675917404335816	24.657499051324454	25.52310891017929	25.143474634160444
22-23	24.231758087755725	25.466276981828962	25.039677490808227	25.26228743960709
24-25	24.140780299563193	24.76741071839235	25.372259740649334	25.719549241395118
26-27	24.086603653052205	24.80396010691077	25.34679586721217	25.76264037282486
28-29	24.697757385684625	24.88588039208684	24.97887406322638	25.437488159002157
30-31	24.224246756925545	24.57073105508665	25.35931365845358	25.84570852953422
32-33	24.32947978996125	25.121326171833502	25.080250364314804	25.468943673890443
34-35	24.965008259955958	24.666739400631872	25.081718720403583	25.286533619008587
36-37	24.730264768391553	25.062679024306977	24.751798684294446	25.455257523007024
38-39	24.47652677760443	25.582211669895198	25.012997955027487	24.92826359747288
40-41	24.982913948795428	24.68309170938717	24.84377211235182	25.49022222946558
42-43	24.306962465324048	24.922389980246482	25.359873712095226	25.410773842334244
44-45	24.39771131766313	24.768089178882448	25.478580243113875	25.355619260340546
46-47	25.05150395051893	24.662680441843456	24.783637987936654	25.502177619700955
48-49	24.49458886195173	24.904988961833343	25.205635903912643	25.394786272302284
50-51	24.84577177117338	24.817153791603907	25.411960006329597	24.925114430893117
52-53	24.787283583409124	24.672079107776806	24.715182583850538	25.82545472496353
54-55	24.81821009341674	24.593985881516957	25.182393786718333	25.40541023834797
56-57	24.485140414944624	24.78597273205292	25.488342802677472	25.240544050324985
58-59	24.732979334058356	24.728113807824144	25.11861625371832	25.420290604399188
60-61	24.78225186433846	24.64484599739817	25.248778056690547	25.324124081572823
62-63	24.580350391977834	24.805459199307155	25.39737236063573	25.216818048079283
64-65	25.050023385767982	24.785423342191145	25.160770926686627	25.003782345354246
66-67	24.555002225904488	25.379310591349896	24.793546406431467	25.27214077631415
68-69	24.611314853119197	25.609786658606026	24.98427298239503	24.79462550587975
70-71	24.86840635836247	25.51347343034417	24.664723650933734	24.95339656035962
72-73	24.630821108387945	25.43320972421928	24.78289195367615	25.153077213716625
74-75	24.694460310739117	25.56645505830829	24.963768757487955	24.775315873464635
76-77	24.830454287072	25.48051834114214	24.73059973650495	24.958427635280913
78-79	24.726748961933197	25.365777250066717	24.767502218296812	25.139971569703274
80-81	24.77920415589237	25.503331324220785	24.88195658149002	24.835507938396827
82-83	24.97400133013112	25.428921290380114	24.604279253473063	24.9927981260157
84-85	24.718589001054347	25.328295131225325	24.801607827629994	25.151508040090338
86-87	24.858786937238737	25.560919290373786	24.90546685927676	24.674826913110717
88-89	24.95717706376319	25.375725508530884	24.607500972412943	25.059596455292983
90-91	24.884878395187954	25.209920566795784	24.704954014051538	25.20024702396472
92-93	24.880263291269774	25.438939874131588	24.91266201559381	24.768134819004832
94-95	25.08330457933055	25.34545085309596	24.640665782748407	24.93057878482508
96-97	24.81586297365895	25.329848270820776	24.709267426157258	25.145021329363015
98-99	24.962392301097832	25.439564561892315	24.84609692449308	24.75194621251677
100-101	25.271674974309814	25.35758479416403	24.456951065327175	24.913789166198985
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	2.0
4	1.5
5	4.0
6	5.5
7	6.0
8	9.0
9	13.0
10	18.0
11	23.0
12	41.5
13	66.0
14	81.5
15	130.5
16	211.5
17	291.0
18	450.0
19	697.0
20	1056.5
21	1615.5
22	2466.0
23	3846.0
24	5964.0
25	9153.0
26	13640.0
27	19637.5
28	27666.5
29	38348.0
30	51479.5
31	67945.5
32	88628.5
33	113847.0
34	146683.5
35	188859.0
36	240110.5
37	302452.5
38	375582.5
39	452803.5
40	529067.5
41	601168.5
42	664312.0
43	705535.5
44	726896.0
45	729217.0
46	712253.5
47	684858.5
48	648596.5
49	611156.5
50	572603.0
51	536283.5
52	510090.5
53	492680.5
54	481818.0
55	472428.0
56	461275.5
57	450048.0
58	432830.5
59	412827.0
60	394022.5
61	372351.5
62	351143.0
63	324524.5
64	288501.5
65	257957.0
66	238362.5
67	219540.0
68	198290.0
69	174252.0
70	148029.5
71	121872.5
72	99145.0
73	81275.0
74	63090.0
75	46276.5
76	33138.0
77	22928.5
78	15621.5
79	10560.0
80	6808.0
81	4041.0
82	2426.5
83	1325.0
84	696.5
85	444.0
86	278.5
87	190.5
88	139.5
89	96.5
90	72.0
91	48.5
92	34.0
93	21.5
94	14.5
95	8.5
96	3.5
97	2.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11582957063491583
2	4.7459129047641176E-4
3	0.0026014633700188494
4	0.0014179147196949585
5	1.9335200723113072E-4
6	0.0
7	0.0
8	1.464787933569172E-4
9	0.0
10-11	8.78872760141503E-6
12-13	8.495770014701197E-5
14-15	2.490139487067592E-4
16-17	6.679432977075425E-4
18-19	0.0014765062370377252
20-21	5.947039010290838E-4
22-23	1.201126105526721E-4
24-25	0.0
26-27	0.0
28-29	1.5526752095833222E-4
30-31	0.022953226919028926
32-33	0.03620662814196279
34-35	0.05695095485716941
36-37	0.04793372033811759
38-39	0.040668372187614495
40-41	0.05591388500020243
42-43	0.06660976649112453
44-45	0.0554685894683974
46-47	0.02522657779192828
48-49	0.018254187228139023
50-51	0.027051703557155467
52-53	0.06262261373594924
54-55	0.06416942979379828
56-57	0.06525630244050662
58-59	0.04994926853470876
60-61	0.0703361869941245
62-63	0.06255523349100506
64-65	0.045677946920421056
66-67	0.013733851665144556
68-69	0.04861631151516082
70-71	0.07960536503775022
72-73	0.07865032330506312
74-75	0.0679368643589382
76-77	0.023427818209505336
78-79	0.014196724652152415
80-81	4.6873213874213505E-4
82-83	0.003389519278279064
84-85	0.007666700044301046
86-87	1.2890133815408715E-4
88-89	0.0013856893851564368
90-91	8.612953049386733E-4
92-93	0.0016815765477374094
94-95	7.49971421987416E-4
96-97	0.0
98-99	2.929575867138344E-6
100-101	8.78872760141503E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.7067317E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.45417115996676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.15666188785426	42.232045633007864
2	12.669779244863348	14.051842136072926
3	4.3468626691165175	7.231549993861719
4	2.0513126443142	4.550153699216146
5	1.2846038709562282	3.561832146638126
6	0.7770419825930088	2.585413146071557
7	0.5705676390711751	2.2148248860773756
8	0.3905646050662056	1.7326749166692947
9	0.267879056723854	1.3369509955561538
>10	1.383816748117024	13.209156836740183
>50	0.06805924079184097	2.5623912831161713
>100	0.030875546145977754	3.133446223602083
>500	0.0016338639559536274	0.5745293832982559
>1k	2.9724362430150496E-4	0.27871202567741693
>5k	3.321504624084086E-5	0.12100557618069685
>10k+	1.0541759825702998E-5	0.6234711182140388
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT	106652	0.6248902507640773	TruSeq Adapter, Index 19 (97% over 40bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.05146092968215215	2.929575867138344E-5	5.859151734276688E-6
2	0.05212887297985969	2.929575867138344E-5	1.1718303468553375E-5
3	0.054548702646115964	3.515491040566013E-5	1.1718303468553375E-5
4	0.062241768873221255	4.1014062139936816E-5	1.7577455202830065E-5
5	0.06368312019985332	4.1014062139936816E-5	1.7577455202830065E-5
6	0.06744469561325896	5.859151734276688E-5	2.343660693710675E-5
7	0.06832356837340046	5.859151734276688E-5	2.343660693710675E-5
8	0.07030396165958598	5.859151734276688E-5	2.343660693710675E-5
9	0.07293472078827622	6.445066907704357E-5	2.343660693710675E-5
10-11	0.07820209819739096	6.738024494418192E-5	3.515491040566013E-5
12-13	0.08347533475823998	9.081685188128866E-5	4.1014062139936816E-5
14-15	0.08857279676706069	1.0839430708411873E-4	4.1014062139936816E-5
16-17	0.09385775163137827	1.201126105526721E-4	4.1014062139936816E-5
18-19	0.09886732636418483	1.2304218641981046E-4	4.1014062139936816E-5
20-21	0.10710236412671072	1.318309140212255E-4	4.394363800707516E-5
22-23	0.11259824845346225	1.464787933569172E-4	5.2732365608490195E-5
24-25	0.11585007766598582	1.6405624855974726E-4	5.859151734276688E-5
26-27	0.11895249850928533	1.6991540029402395E-4	6.738024494418192E-5
28-29	0.12145142672395431	1.6991540029402395E-4	7.616897254559695E-5
30-31	0.12351384813441972	1.8163370376257732E-4	9.3746427748427E-5
32-33	0.1253682696583183	1.9042243136399236E-4	1.2304218641981046E-4
34-35	0.12700004341631435	2.0799988656682245E-4	1.4354921748977888E-4
36-37	0.1286757608123175	2.1092946243396078E-4	1.523379450911939E-4
38-39	0.1303046049944464	2.1092946243396078E-4	1.5526752095833225E-4
40-41	0.13191001256963822	2.138590383010991E-4	1.87492855496854E-4
42-43	0.1332898428030604	2.1678861416823747E-4	2.2557734176965248E-4
44-45	0.13464623642954543	2.3436606937106752E-4	2.2850691763679084E-4
46-47	0.13607879902857606	2.490139487067593E-4	2.2850691763679084E-4
48-49	0.13756995314494952	2.607322521753126E-4	2.2850691763679084E-4
50-51	0.13909333259586143	2.8709843497955775E-4	2.314364935039292E-4
52-53	0.14062257119850766	2.929575867138344E-4	2.3436606937106752E-4
54-55	0.14204927464580402	3.134646177838028E-4	2.3729564523820585E-4
56-57	0.14346425978963184	3.222533453852178E-4	2.402252211053442E-4
58-59	0.14499056881641093	3.310420729866329E-4	2.4315479697248257E-4
60-61	0.14669851154695257	3.57408255790878E-4	2.460843728396209E-4
62-63	0.14872284847114517	3.691265592594313E-4	2.460843728396209E-4
64-65	0.1508555797024219	3.8670401446226144E-4	2.490139487067593E-4
66-67	0.15330470512734956	3.9842231793081476E-4	2.519435245738976E-4
68-69	0.15571281649013727	4.159997731336449E-4	2.519435245738976E-4
70-71	0.15851056144325437	4.540842594064433E-4	2.578026763081743E-4
72-73	0.16197624969407903	4.658025628749967E-4	2.783097073781427E-4
74-75	0.16569681104534473	4.863095939449651E-4	2.870984349795577E-4
76-77	0.17014976636339502	4.980278974135185E-4	2.870984349795577E-4
78-79	0.17488982011642487	5.038870491477952E-4	2.9588716258097277E-4
80-81	0.18129094338612214	5.185349284834869E-4	3.193237695180795E-4
82-83	0.1901734174152856	5.273236560849019E-4	3.310420729866329E-4
84-85	0.20139076341055834	5.390419595534553E-4	3.3690122472090954E-4
86-87	0.21554061484883652	5.449011112877319E-4	3.4568995232232456E-4
88-89	0.23306533768605808	5.624785664905621E-4	3.57408255790878E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGAA	25005	0.0	45.459816	2
AGAGCAC	26540	0.0	43.169334	8
GAGCACA	26945	0.0	42.855156	9
TCGGAAG	27755	0.0	40.98922	3
GATCGGA	28090	0.0	40.54492	1
CGGAAGA	29330	0.0	38.96563	4
AGTCACG	17325	0.0	35.09994	28-29
TATGCCG	18420	0.0	33.197525	48-49
CACACGT	18890	0.0	32.677357	12-13
TCACGTG	18775	0.0	32.61814	30-31
CGTATGC	19015	0.0	32.216007	46-47
CGTCTGA	19305	0.0	31.864534	16-17
CTCGTAT	18780	0.0	30.923634	44-45
ACGTCTG	18510	0.0	30.834486	14-15
GTCACGT	18625	0.0	30.481848	28-29
CACGTGA	18640	0.0	30.458479	30-31
TGAAACG	18715	0.0	29.983385	34-35
AAGAGCA	38390	0.0	29.930567	7
CACGTCT	20865	0.0	29.482052	14-15
ACACGTC	19455	0.0	29.336529	12-13
>>END_MODULE