##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	C5B16ACXX l06n01 k48-1.3410000000caca.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13484103
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79964295734021	33.0	33.0	33.0	33.0	33.0
2	32.80582208545871	33.0	33.0	33.0	33.0	33.0
3	32.72340407070459	33.0	33.0	33.0	33.0	33.0
4	36.71993064722214	37.0	37.0	37.0	37.0	37.0
5	36.73870060173821	37.0	37.0	37.0	37.0	37.0
6	36.68893051321248	37.0	37.0	37.0	37.0	37.0
7	36.7042886723722	37.0	37.0	37.0	37.0	37.0
8	36.70539634709109	37.0	37.0	37.0	37.0	37.0
9	36.704775616145916	37.0	37.0	37.0	37.0	37.0
10-11	36.6871057348049	37.0	37.0	37.0	37.0	37.0
12-13	36.68564846323111	37.0	37.0	37.0	37.0	37.0
14-15	39.11652677230365	40.0	40.0	40.0	37.0	40.0
16-17	39.10472035106822	40.0	40.0	40.0	37.0	40.0
18-19	39.07338671322816	40.0	40.0	40.0	37.0	40.0
20-21	39.07257968883803	40.0	40.0	40.0	37.0	40.0
22-23	39.03926334588218	40.0	40.0	40.0	37.0	40.0
24-25	39.012511325373296	40.0	40.0	40.0	37.0	40.0
26-27	38.95758271796055	40.0	40.0	40.0	37.0	40.0
28-29	38.91451930469531	40.0	40.0	40.0	37.0	40.0
30-31	38.85687435048516	40.0	40.0	40.0	37.0	40.0
32-33	38.790526666846134	40.0	40.0	40.0	37.0	40.0
34-35	38.71618367940381	40.0	38.5	40.0	37.0	40.0
36-37	38.64582404925267	40.0	37.0	40.0	37.0	40.0
38-39	38.559421342302116	40.0	37.0	40.0	37.0	40.0
40-41	38.4545594171151	40.0	37.0	40.0	37.0	40.0
42-43	38.33211030055169	40.0	37.0	40.0	37.0	40.0
44-45	38.216365597325975	40.0	37.0	40.0	37.0	40.0
46-47	38.122388267132045	40.0	37.0	40.0	37.0	40.0
48-49	37.990934361744344	40.0	37.0	40.0	37.0	40.0
50-51	37.869263680350116	40.0	37.0	40.0	33.0	40.0
52-53	37.713401106473306	40.0	37.0	40.0	33.0	40.0
54-55	37.534728746880674	40.0	37.0	40.0	33.0	40.0
56-57	37.36330911296065	37.0	37.0	40.0	33.0	40.0
58-59	37.20570548890052	37.0	37.0	40.0	33.0	40.0
60-61	37.02494029450828	37.0	37.0	40.0	33.0	40.0
62-63	36.82673630570754	37.0	37.0	40.0	33.0	40.0
64-65	36.64652264967124	37.0	37.0	40.0	33.0	40.0
66-67	36.39608967685874	37.0	37.0	40.0	33.0	40.0
68-69	36.19873398326904	37.0	37.0	37.0	33.0	40.0
70-71	35.98587277181137	37.0	37.0	37.0	33.0	40.0
72-73	35.71080252798425	37.0	37.0	37.0	33.0	40.0
74-75	35.44482892187934	37.0	37.0	37.0	33.0	38.5
76-77	32.87172873123262	33.0	33.0	37.0	27.0	37.0
78-79	34.9233345369729	37.0	33.0	37.0	33.0	37.0
80-81	35.11807474327362	37.0	37.0	37.0	33.0	37.0
82-83	35.071442609122755	37.0	35.0	37.0	33.0	37.0
84-85	34.97942536481663	37.0	33.0	37.0	33.0	37.0
86-87	34.92198179589699	37.0	33.0	37.0	33.0	37.0
88-89	34.84990970478347	37.0	33.0	37.0	33.0	37.0
90-91	34.77667520783548	37.0	33.0	37.0	33.0	37.0
92-93	34.70287270870001	37.0	33.0	37.0	33.0	37.0
94-95	34.64409512445878	37.0	33.0	37.0	33.0	37.0
96-97	34.57808810122557	37.0	33.0	37.0	33.0	37.0
98-99	34.53117100188273	37.0	33.0	37.0	33.0	37.0
100-101	32.80641296643907	35.0	30.0	37.0	27.5	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2306	1	0.054622033366037215
2306	2	0.05255200961652662
2306	3	0.13196690172249248
2306	4	0.15582944702111234
2306	5	0.13575910140096425
2306	6	0.13247671535738448
2306	7	0.12446365279303961
2306	8	0.14338690365190843
2306	9	0.13286820801382504
2306	10-11	0.16537525431520805
2306	12-13	0.14526844777544312
2306	14-15	0.3123503387854072
2306	16-17	0.30073298024978357
2306	18-19	0.3382640119017637
2306	20-21	0.3420863111507231
2306	22-23	0.3440650579150031
2306	24-25	0.34376699171875913
2306	26-27	0.3361187004031265
2306	28-29	0.3474898554342829
2306	30-31	0.3442519938102322
2306	32-33	0.3268953368291463
2306	34-35	0.3856084958285848
2306	36-37	0.30907055891331936
2306	38-39	0.34304918387277894
2306	40-41	0.3477186073634826
2306	42-43	0.35472235311652156
2306	44-45	0.3895069003953111
2306	46-47	0.3679227449726241
2306	48-49	0.34800554978242104
2306	50-51	0.33821736035945094
2306	52-53	0.36849151354461895
2306	54-55	0.3524182335228616
2306	56-57	0.29100543079375285
2306	58-59	0.35907848966097333
2306	60-61	0.3274930067515669
2306	62-63	0.3035926497685182
2306	64-65	0.3277853446024608
2306	66-67	0.3591792162641383
2306	68-69	0.36441193557456586
2306	70-71	0.3182003204545154
2306	72-73	0.30649520755088133
2306	74-75	0.39241688370734806
2306	76-77	0.011082429733697552
2306	78-79	0.3510907029423649
2306	80-81	0.4210220294941607
2306	82-83	0.39364618874738255
2306	84-85	0.45091840814758655
2306	86-87	0.37391223678903174
2306	88-89	0.3219027977277378
2306	90-91	0.37599011186694753
2306	92-93	0.3800507718533339
2306	94-95	0.3549471765689418
2306	96-97	0.28544613482209513
2306	98-99	0.3458037564506853
2306	100-101	0.6018561353706957
2307	1	0.040305599326636354
2307	2	0.057581113949709106
2307	3	0.12772916436997406
2307	4	0.14035965086660696
2307	5	0.1306940742801217
2307	6	0.14278567883198434
2307	7	0.15292876660983978
2307	8	0.1620950445806102
2307	9	0.1529508854268471
2307	10-11	0.15563385369774352
2307	12-13	0.15782473397108276
2307	14-15	0.35340548731151955
2307	16-17	0.3476012952166556
2307	18-19	0.3582362224457398
2307	20-21	0.37042769760532224
2307	22-23	0.3491515097989293
2307	24-25	0.3793612835362339
2307	26-27	0.37385258095453366
2307	28-29	0.3758644575765189
2307	30-31	0.36288389764578
2307	32-33	0.399155304715201
2307	34-35	0.41203014444734976
2307	36-37	0.45498471995122003
2307	38-39	0.426717215032447
2307	40-41	0.3789067544248397
2307	42-43	0.36472647134615244
2307	44-45	0.44574072439839796
2307	46-47	0.47451008186340005
2307	48-49	0.4587559338468594
2307	50-51	0.46992136896441394
2307	52-53	0.5042903418085913
2307	54-55	0.4559766176780542
2307	56-57	0.46118295983534807
2307	58-59	0.45413945011600276
2307	60-61	0.4381449640966082
2307	62-63	0.46270087258055526
2307	64-65	0.45692547166256503
2307	66-67	0.48224175766557664
2307	68-69	0.45458516247561676
2307	70-71	0.4672401507476991
2307	72-73	0.4697033118692815
2307	74-75	0.5048065500448118
2307	76-77	0.32648714376595933
2307	78-79	0.5772410399806134
2307	80-81	0.5617381373752792
2307	82-83	0.6089913551730177
2307	84-85	0.5974741432979869
2307	86-87	0.6040446664035741
2307	88-89	0.6190154306205642
2307	90-91	0.6062267863174569
2307	92-93	0.5997086923811281
2307	94-95	0.6489613229283506
2307	96-97	0.5865481651643023
2307	98-99	0.6424708608368022
2307	100-101	0.71888131947928
2201	1	0.05374739777855808
2201	2	0.056781897437211626
2201	3	0.10963136127906381
2201	4	0.11319087432336516
2201	5	0.10584216739058405
2201	6	0.12176799385450465
2201	7	0.10588406957280228
2201	8	0.10444465659359992
2201	9	0.11208355705859674
2201	10-11	0.12205054115398184
2201	12-13	0.11102746293850174
2201	14-15	0.25788750858640697
2201	16-17	0.2236453300821708
2201	18-19	0.26442599035387815
2201	20-21	0.27044735385268126
2201	22-23	0.2803305376217651
2201	24-25	0.28867848519268335
2201	26-27	0.2818763015275465
2201	28-29	0.30225550064339757
2201	30-31	0.2874495345537582
2201	32-33	0.2918411561887595
2201	34-35	0.273010894477828
2201	36-37	0.27572572594646516
2201	38-39	0.31573784046646836
2201	40-41	0.2864804831904664
2201	42-43	0.3113492933625892
2201	44-45	0.32028399138219044
2201	46-47	0.31582488465245007
2201	48-49	0.3121046110597874
2201	50-51	0.30759541035592264
2201	52-53	0.3204613168599053
2201	54-55	0.3449404407266172
2201	56-57	0.33794925684019717
2201	58-59	0.3657471583565979
2201	60-61	0.390409934322669
2201	62-63	0.3574627154874861
2201	64-65	0.3924783476413438
2201	66-67	0.3513382353818173
2201	68-69	0.3470232798979893
2201	70-71	0.2407068789205553
2201	72-73	0.2780634108820905
2201	74-75	0.2835285125621425
2201	76-77	0.08347731882304998
2201	78-79	0.3387488708725286
2201	80-81	0.35096907849501946
2201	82-83	0.3891850073928964
2201	84-85	0.4338445094082317
2201	86-87	0.4101338448735916
2201	88-89	0.37630050669894644
2201	90-91	0.34870011563099723
2201	92-93	0.35552936442046956
2201	94-95	0.4440818310982877
2201	96-97	0.3204615562339015
2201	98-99	0.3677844538475128
2201	100-101	0.38394913761605665
1101	1	0.04405929576945056
1101	2	0.06758109189374295
1101	3	0.13964268156146886
1101	4	0.13102455321899953
1101	5	0.12065228189186428
1101	6	0.13460161183232344
1101	7	0.13880568534909088
1101	8	0.13449699062790899
1101	9	0.12182190033416873
1101	10-11	0.15370809176658184
1101	12-13	0.15146613550302845
1101	14-15	0.27679852299176844
1101	16-17	0.2644090001146324
1101	18-19	0.3174119381359475
1101	20-21	0.3097411770321017
1101	22-23	0.2674188451331787
1101	24-25	0.3255176967599951
1101	26-27	0.34029999697811064
1101	28-29	0.33293747343824265
1101	30-31	0.37511354275724784
1101	32-33	0.34483441295065376
1101	34-35	0.35769729038208453
1101	36-37	0.38235348217811804
1101	38-39	0.3868085981262155
1101	40-41	0.3358655846888894
1101	42-43	0.3829920467977601
1101	44-45	0.3975683556404661
1101	46-47	0.3578387380035295
1101	48-49	0.372628609384968
1101	50-51	0.3579215792313377
1101	52-53	0.35380567104511584
1101	54-55	0.38426879753151155
1101	56-57	0.4378707255906491
1101	58-59	0.4414354408122989
1101	60-61	0.41076704864192237
1101	62-63	0.37703775737443834
1101	64-65	0.39475717278259737
1101	66-67	0.3658587496710908
1101	68-69	0.4242630889399095
1101	70-71	0.3886626615981683
1101	72-73	0.4264058333752274
1101	74-75	0.42026272173730916
1101	76-77	0.3747752833944702
1101	78-79	0.5057682001622865
1101	80-81	0.43589759812149964
1101	82-83	0.42727372992222
1101	84-85	0.4853914981670897
1101	86-87	0.48847648101382646
1101	88-89	0.512799941704948
1101	90-91	0.5058645231273573
1101	92-93	0.5427112013703166
1101	94-95	0.5232886541173087
1101	96-97	0.5066853719876079
1101	98-99	0.4899551874658741
1101	100-101	0.5874236759183518
2304	1	0.04025761616017576
2304	2	0.05515669909700449
2304	3	0.12633694586862987
2304	4	0.1329124655633862
2304	5	0.11034252656506283
2304	6	0.13600436419781659
2304	7	0.1179971627622507
2304	8	0.13095579276749447
2304	9	0.12257392288707081
2304	10-11	0.1285216827640383
2304	12-13	0.13052737204176168
2304	14-15	0.2708795643881814
2304	16-17	0.28177317501762644
2304	18-19	0.2947982080753846
2304	20-21	0.28535318306918356
2304	22-23	0.2928305155594444
2304	24-25	0.2833604083911254
2304	26-27	0.3124679069051055
2304	28-29	0.28400520103716786
2304	30-31	0.29511821859846776
2304	32-33	0.2733347572350411
2304	34-35	0.23369426143412397
2304	36-37	0.2885619335581495
2304	38-39	0.25561679778382995
2304	40-41	0.299099950623841
2304	42-43	0.28628826900050086
2304	44-45	0.2848275573935197
2304	46-47	0.31735442079669696
2304	48-49	0.26634542916571036
2304	50-51	0.29395348701628876
2304	52-53	0.28860051064997805
2304	54-55	0.30526851616914286
2304	56-57	0.2935152258082283
2304	58-59	0.29695350806722587
2304	60-61	0.31592403037669925
2304	62-63	0.3045980563149513
2304	64-65	0.35827152314970334
2304	66-67	0.30438398203232
2304	68-69	0.30531385646568765
2304	70-71	0.3481529983359195
2304	72-73	0.38051815224999075
2304	74-75	0.3840004695953425
2304	76-77	0.21500136586342933
2304	78-79	0.3701354255685416
2304	80-81	0.38604462868502054
2304	82-83	0.37522309224279127
2304	84-85	0.41967114903533087
2304	86-87	0.4212001253615014
2304	88-89	0.4732133711463149
2304	90-91	0.43450381045094133
2304	92-93	0.3901792001886051
2304	94-95	0.46373158744209064
2304	96-97	0.384441209484919
2304	98-99	0.4155348920050912
2304	100-101	0.4429058195851425
2202	1	0.04821701175927018
2202	2	0.057465180999891174
2202	3	0.12769222017187332
2202	4	0.1091495608146218
2202	5	0.09452765368557436
2202	6	0.1159947797888492
2202	7	0.11328450438961113
2202	8	0.10969829139749265
2202	9	0.1261564882341233
2202	10-11	0.12633618333185126
2202	12-13	0.11499861096492481
2202	14-15	0.2675689809428832
2202	16-17	0.23675822647962264
2202	18-19	0.27674041902358937
2202	20-21	0.2740121059520746
2202	22-23	0.26651293968225787
2202	24-25	0.26490025359020564
2202	26-27	0.2689001891681855
2202	28-29	0.3158279059188587
2202	30-31	0.3335528947209525
2202	32-33	0.2883115550406501
2202	34-35	0.310773020305497
2202	36-37	0.29580810558856996
2202	38-39	0.3088719456655795
2202	40-41	0.30977019387494664
2202	42-43	0.29911612568891854
2202	44-45	0.3260900827282782
2202	46-47	0.32487668660480296
2202	48-49	0.313877892426099
2202	50-51	0.3033782056705334
2202	52-53	0.31509190673651943
2202	54-55	0.3417684425291796
2202	56-57	0.3497280086585661
2202	58-59	0.38428571549417256
2202	60-61	0.39760823980126503
2202	62-63	0.38263989128817855
2202	64-65	0.353051758334054
2202	66-67	0.36854232962639344
2202	68-69	0.3501469853266528
2202	70-71	0.33181487055526304
2202	72-73	0.35680249669361075
2202	74-75	0.4187654086012742
2202	76-77	0.4275412417146853
2202	78-79	0.4297842414604176
2202	80-81	0.3987035246538113
2202	82-83	0.4164883837486286
2202	84-85	0.4491553179392298
2202	86-87	0.4375209832632905
2202	88-89	0.3431139160820109
2202	90-91	0.44465962064375475
2202	92-93	0.44857329583383176
2202	94-95	0.42181759143883113
2202	96-97	0.47619136592310696
2202	98-99	0.5179543197964378
2202	100-101	0.6239511930144985
1102	1	0.03299601865575141
1102	2	0.048288516310620366
1102	3	0.1141180370654169
1102	4	0.10303171176364145
1102	5	0.09289299549991625
1102	6	0.09661190016669963
1102	7	0.09274245520222024
1102	8	0.08357599103781865
1102	9	0.10187882413458027
1102	10-11	0.0896881276652266
1102	12-13	0.09373392099291777
1102	14-15	0.22019176868166568
1102	16-17	0.21769673831391856
1102	18-19	0.21452203398626324
1102	20-21	0.23958251807705722
1102	22-23	0.24734291275662912
1102	24-25	0.22986213871508454
1102	26-27	0.26136963759790177
1102	28-29	0.23881453805521602
1102	30-31	0.239121560295267
1102	32-33	0.23052505322049655
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1213	52-53	-0.6463528360769004
1213	54-55	-0.7053576231343683
1213	56-57	-1.1551132287758037
1213	58-59	-1.0411377100329062
1213	60-61	-0.690820987893936
1213	62-63	-0.6805196872448818
1213	64-65	-0.6699038118688279
1213	66-67	-0.6134541260494188
1213	68-69	-0.6780943968925186
1213	70-71	-0.5967744440739651
1213	72-73	-0.5129412347608593
1213	74-75	-0.5002226366659741
1213	76-77	-0.2983719839106769
1213	78-79	-0.6507635013854767
1213	80-81	-0.6551983309651135
1213	82-83	-0.6724366224064795
1213	84-85	-0.6018786880936275
1213	86-87	-0.5763215595954208
1213	88-89	-0.623152962236496
1213	90-91	-0.6050925724917775
1213	92-93	-0.7356852936338569
1213	94-95	-0.669387154131087
1213	96-97	-0.7525133152987777
1213	98-99	-0.6900675913450698
1213	100-101	-0.3512490597926359
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	59.0
8	540.0
9	2009.0
10	2337.0
11	1584.0
12	1209.0
13	1065.0
14	1262.0
15	1605.0
16	2553.0
17	3894.0
18	5723.0
19	8301.0
20	11185.0
21	15352.0
22	20519.0
23	26154.0
24	33505.0
25	43364.0
26	54885.0
27	69569.0
28	89235.0
29	116825.0
30	154646.0
31	179182.0
32	220563.0
33	298683.0
34	428190.0
35	696676.0
36	1606456.0
37	4616263.0
38	4767527.0
39	3180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.924735692718475	13.16562954370079	14.18107230726133	50.7285624563194
2	23.066783437012873	18.705634975096814	37.476843881166864	20.75073770672345
3	24.851578983357104	23.188542730368233	25.919255080793054	26.040623205481612
4	26.95187136427679	27.781497438501866	18.65757592792474	26.609055269296594
5	29.093031325628587	29.78080581153867	21.449449502305352	19.676713360527394
6	21.753089545518897	33.240208859276734	22.178338447874506	22.82836314732986
7	19.556221129429225	16.155646393386345	40.918124105103615	23.370008372080814
8	21.29406836017499	21.306275348876255	27.118678257521335	30.280978033427424
9	22.363652962306798	20.432608680013793	30.340320004971783	26.86341835270763
10-11	25.277233886246187	28.713677039820674	21.111376196946598	24.897712876986546
12-13	23.110969759319282	23.099218871902945	27.64943391054701	26.140377458230766
14-15	23.617788998585777	24.840457174835606	26.22417087474777	25.317582951830847
16-17	24.833967324649347	24.638639782245907	25.16827421797695	25.359118675127796
18-19	24.409774141113118	24.972719329250737	25.408620469469305	25.20888606016684
20-21	24.547670940628112	24.883145908342666	25.619284135405795	24.949899015623423
22-23	24.270237839645777	25.510210741032857	25.223001783213324	24.99654963610805
24-25	24.1612512155981	25.07721499902515	25.430623750055897	25.33091003532085
26-27	24.162404425418586	25.01224219364091	25.376141075160874	25.44921230577963
28-29	24.574205411357568	25.170953399578348	25.012069785385492	25.24277140367859
30-31	24.30777951874642	24.893904391817788	25.453154405967332	25.345161683468458
32-33	24.332707528325923	25.317660461636667	25.092342467326628	25.25728954271078
34-35	24.731060798877095	24.881901531158093	25.167462300576425	25.219575369388387
36-37	24.255094158526084	24.917331613546967	25.663656471684256	25.163917756242686
38-39	24.649770279989394	25.016162679427257	25.189200169572736	25.144866871010613
40-41	25.01987983242803	25.100512202727725	24.926621871729864	24.95298609311438
42-43	24.24589616480646	25.31096755521254	25.44206022916172	25.001076050819282
44-45	24.460532762123236	24.929529050333528	25.463775221628666	25.14616296591457
46-47	24.831203578202256	24.819293991069923	25.049081148091616	25.300421282636197
48-49	24.37263956829004	25.113624866522215	25.39168071787091	25.12205484731684
50-51	24.515942358393858	25.108251039596464	25.563887938336872	24.811918663672806
52-53	24.702592863505092	24.851590479142523	24.93391261720432	25.511904040148064
54-55	24.6468317263859	24.883651346307943	25.30904357451323	25.160473352792923
56-57	24.33339544678009	24.922089181700237	25.563876184821417	25.180639186698254
58-59	24.60813030992793	24.85561485025959	25.32666983616842	25.209585003644065
60-61	24.618466191081385	24.772043748898394	25.432756013618192	25.176734046402032
62-63	24.530863801298597	24.925481593809895	25.420231494510766	25.123423110380745
64-65	24.78513385516436	24.927574168310464	25.313071165400373	24.9742208111248
66-67	24.54123027203018	25.422993069259785	24.9326039993016	25.103172659408436
68-69	24.517697183120276	25.635989822931155	25.051469834531183	24.794843159417386
70-71	24.664788889198142	25.5368161281045	24.848543887481792	24.949851095215564
72-73	24.52204139146803	25.585074225747373	25.016481395311125	24.876402987473472
74-75	24.67775499943989	25.553648860787703	25.03596929566303	24.73262684410938
76-77	24.823495433366812	25.456113466153756	24.855618698878303	24.864772401601126
78-79	24.623707276043433	25.409577375782423	24.977548157977303	24.989167190196845
80-81	24.74559924177027	25.506921516103482	24.897986805141485	24.84949243698476
82-83	24.779118132320757	25.442789980421804	24.802568805420712	24.97552308183673
84-85	24.581242984683378	25.363276031042947	24.979730027401917	25.075750956871758
86-87	24.714438280427185	25.54680494922847	25.023443383963528	24.71531338638082
88-89	24.867518174418798	25.42517849958376	24.831760781819835	24.875542544177602
90-91	24.77875141431674	25.291051765893087	24.942108172847576	24.9880886469426
92-93	24.748305255759597	25.500172687516876	25.074776253415838	24.676745803307696
94-95	24.869440793104932	25.337980712201	24.918843728795498	24.873734765898572
96-97	24.631471592882374	25.44357974720306	24.94167761845189	24.983271041462675
98-99	24.86313512015854	25.379539549611827	24.978051931081506	24.77927339914812
100-101	25.03375827326136	25.310747426858683	24.787334744278855	24.868159555601107
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.0
2	1.0
3	1.5
4	2.5
5	2.5
6	7.5
7	9.0
8	7.5
9	12.5
10	18.5
11	33.5
12	54.5
13	78.0
14	115.5
15	156.0
16	218.5
17	326.5
18	466.5
19	683.0
20	1013.0
21	1556.0
22	2419.5
23	3762.5
24	5678.0
25	8472.0
26	12363.0
27	17562.5
28	24530.0
29	33471.5
30	44609.0
31	58832.5
32	75874.0
33	96275.0
34	122794.0
35	157445.5
36	200124.0
37	252511.5
38	311342.5
39	369734.0
40	427552.5
41	482033.0
42	527504.0
43	560725.5
44	580053.0
45	578555.5
46	563358.0
47	546110.5
48	517514.5
49	484653.5
50	458837.5
51	428658.0
52	399611.5
53	379536.0
54	366220.0
55	357936.0
56	353259.5
57	349156.5
58	333061.5
59	308329.0
60	290997.5
61	276644.5
62	261755.0
63	243651.0
64	215145.0
65	193401.5
66	184405.0
67	173354.5
68	157558.0
69	137365.5
70	116587.0
71	97312.5
72	79568.5
73	66384.0
74	53974.0
75	41018.5
76	28821.0
77	19362.0
78	13552.0
79	10573.5
80	7679.5
81	4791.0
82	2488.0
83	986.5
84	516.5
85	306.5
86	213.0
87	159.5
88	97.5
89	63.0
90	48.5
91	36.5
92	20.5
93	12.0
94	8.0
95	5.5
96	3.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11564729222255273
2	6.377880679196829E-4
3	0.002462158587782962
4	0.0014609796439555526
5	2.3731649038871922E-4
6	0.0
7	0.0
8	1.779873677915394E-4
9	0.0
10-11	7.4161403246474756E-6
12-13	1.1124210486971213E-4
14-15	2.0394385892780558E-4
16-17	6.340799977573592E-4
18-19	0.0014609796439555526
20-21	6.414961380820066E-4
22-23	1.3349052584365456E-4
24-25	0.0
26-27	0.0
28-29	1.4090666616830202E-4
30-31	0.02300486728705647
32-33	0.036568987940836704
34-35	0.05663706365933277
36-37	0.04790826649722269
38-39	0.04094451073237872
40-41	0.05566184120664163
42-43	0.06602589731033648
44-45	0.05525395348878601
46-47	0.02497014447308805
48-49	0.018236289058308142
50-51	0.026935421659119635
52-53	0.06217692048184443
54-55	0.0642015267904732
56-57	0.06496538924391189
58-59	0.04965476754367717
60-61	0.07029017799700878
62-63	0.06233636749882436
64-65	0.04580949878534746
66-67	0.013712443460273184
68-69	0.048564594915953996
70-71	0.07956035340281811
72-73	0.07844422428395867
74-75	0.06725326853406563
76-77	0.02387626377520255
78-79	0.014187076441050621
80-81	4.67216840452791E-4
82-83	0.0035708715663177594
84-85	0.00819483505873546
86-87	1.5944701697992073E-4
88-89	0.0013200729777872507
90-91	8.788126284707258E-4
92-93	0.00155738946817597
94-95	6.414961380820066E-4
96-97	0.0
98-99	7.4161403246474756E-6
100-101	7.4161403246474756E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.3484103E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.285219629361166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4215700156747	45.125332124135625
2	13.428711192436355	15.65390762380827
3	4.009041584416819	7.01003607752929
4	1.8976440982185607	4.424184121721193
5	0.86292135013132	2.5147780207634423
6	0.5571185498645825	1.9483066223069037
7	0.3688368388390421	1.5048415311393
8	0.22756824997765104	1.0611092344493402
9	0.1717569217566657	0.9009800916705234
>10	0.9367927662896196	9.76457917107151
>50	0.0706670708790764	2.841896903752291
>100	0.043994107608908976	4.9336670267759
>500	0.002555588968545259	0.992458395438401
>1k	7.962197524998546E-4	0.7309514066979993
>5k	0.0	0.0
>10k+	2.5445185591657295E-5	0.59297164873997
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT	56768	0.42099945394958793	TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTA	23197	0.1720322071108475	TruSeq Adapter, Index 21 (97% over 40bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.19718033895172707	1.4832280649294951E-5	7.4161403246474756E-6
2	0.1974324877227651	1.4832280649294951E-5	2.2248420973942427E-5
3	0.1978700400019193	2.9664561298589902E-5	2.2248420973942427E-5
4	0.1994348456104199	2.9664561298589902E-5	2.9664561298589902E-5
5	0.19978340420567833	2.9664561298589902E-5	2.9664561298589902E-5
6	0.20078458314950576	3.708070162323738E-5	3.708070162323738E-5
7	0.2009774027979466	4.449684194788485E-5	4.449684194788485E-5
8	0.20151878104164586	4.449684194788485E-5	4.449684194788485E-5
9	0.2021194884079423	7.416140324647476E-5	5.9329122597179805E-5
10-11	0.20344326945589186	7.786947340879849E-5	6.674526292182728E-5
12-13	0.2048708764683865	8.157754357112223E-5	8.528561373344598E-5
14-15	0.20633556418250437	8.157754357112223E-5	9.270175405809344E-5
16-17	0.20793003435230362	9.640982422041718E-5	9.640982422041718E-5
18-19	0.2094132624172331	9.640982422041718E-5	9.640982422041718E-5
20-21	0.21128583784920657	1.0011789438274093E-4	1.1124210486971213E-4
22-23	0.21254658170439666	1.1124210486971213E-4	1.1865824519435961E-4
24-25	0.21378507713861278	1.1865824519435961E-4	1.334905258436546E-4
26-27	0.21479367222276483	1.260743855190071E-4	1.5573894681759697E-4
28-29	0.21587272064000104	1.4090666616830205E-4	1.6315508714224446E-4
30-31	0.2168368188822052	1.6315508714224446E-4	1.7057122746689195E-4
32-33	0.21765259431791645	1.7057122746689195E-4	1.854035081161869E-4
34-35	0.2185647795778481	1.779873677915394E-4	2.150680694147768E-4
36-37	0.21942875992566951	2.0765192909012932E-4	2.1877613957710054E-4
38-39	0.22036690167673742	2.26192279901748E-4	2.336084202263955E-4
40-41	0.22172034728598558	2.3731649038871922E-4	2.5956491136266166E-4
42-43	0.22381169885753616	2.3731649038871922E-4	3.040617533105465E-4
44-45	0.2246311823634097	2.5956491136266166E-4	3.2260210412216517E-4
46-47	0.22553224341285438	2.5956491136266166E-4	3.337263146091364E-4
48-49	0.22663354025106452	2.818133323366041E-4	3.411424549337839E-4
50-51	0.22791282445706623	2.96645612985899E-4	3.559747355830788E-4
52-53	0.22905491006706194	3.11477893635194E-4	3.5968280574540253E-4
54-55	0.23087557251676288	3.1889403395984146E-4	3.633908759077263E-4
56-57	0.23289647075522935	3.337263146091364E-4	3.708070162323738E-4
58-59	0.23432778583788627	3.411424549337839E-4	3.7451508639469756E-4
60-61	0.23625969039245695	3.596828057454026E-4	3.7822315655702127E-4
62-63	0.2391742335400434	3.8563929688166873E-4	3.930554372063162E-4
64-65	0.241517733882632	4.004715775309637E-4	4.041796476932874E-4
66-67	0.24763604965046618	4.1901192834258234E-4	4.301361388295536E-4
68-69	0.25020574227295656	4.301361388295536E-4	4.3755227915420107E-4
70-71	0.25261598787846695	4.4496841947884853E-4	4.635087702904672E-4
72-73	0.2556788538325464	4.52384559803496E-4	5.005894719137046E-4
74-75	0.2589345394350666	4.7092491061511473E-4	5.042975420760284E-4
76-77	0.26264631766755264	5.080056122383521E-4	5.302540332122944E-4
78-79	0.2673555667737038	5.191298227253233E-4	5.450863138615895E-4
80-81	0.27262844254452817	5.191298227253233E-4	5.599185945108845E-4
82-83	0.27943645936255457	5.413782436992658E-4	5.969992961341217E-4
84-85	0.2888178768732336	5.562105243485607E-4	6.118315767834168E-4
86-87	0.2994674543794274	5.747508751601793E-4	6.340799977573592E-4
88-89	0.31196735889662075	6.044154364587693E-4	6.452042082443303E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	11960	0.0	36.168415	46-47
ACGTTTC	12190	0.0	35.841812	32-33
TATGCCG	12070	0.0	35.74609	48-49
AGTCACG	12355	0.0	35.53397	28-29
ATCGGAA	18380	0.0	34.17384	2
CTCGTAT	12740	0.0	33.48763	44-45
GAGCACA	19080	0.0	33.267082	9
CACACGT	13220	0.0	32.986053	12-13
AGAGCAC	19255	0.0	32.866234	8
GTTTCGG	13930	0.0	31.33255	34-35
GATCGGA	20260	0.0	31.109476	1
TCGGAAG	20365	0.0	30.912811	3
CACGTCT	14365	0.0	30.473278	14-15
CGTCTGA	14790	0.0	29.58156	16-17
CGGAAGA	21850	0.0	28.984734	4
TTCGGAA	15550	0.0	28.052992	36-37
AGCACAC	16190	0.0	27.241623	10-11
TCACGTT	16280	0.0	27.216253	30-31
CGGAATC	16490	0.0	25.777185	38-39
CGTTTCG	12015	0.0	25.745369	32-33
>>END_MODULE