FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n01 k48-1.3410000000caca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n01 k48-1.3410000000caca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13484103
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT567680.42099945394958793TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTA231970.1720322071108475TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC119600.036.16841546-47
ACGTTTC121900.035.84181232-33
TATGCCG120700.035.7460948-49
AGTCACG123550.035.5339728-29
ATCGGAA183800.034.173842
CTCGTAT127400.033.4876344-45
GAGCACA190800.033.2670829
CACACGT132200.032.98605312-13
AGAGCAC192550.032.8662348
GTTTCGG139300.031.3325534-35
GATCGGA202600.031.1094761
TCGGAAG203650.030.9128113
CACGTCT143650.030.47327814-15
CGTCTGA147900.029.5815616-17
CGGAAGA218500.028.9847344
TTCGGAA155500.028.05299236-37
AGCACAC161900.027.24162310-11
TCACGTT162800.027.21625330-31
CGGAATC164900.025.77718538-39
CGTTTCG120150.025.74536932-33
Produced by FastQC (version 0.11.2)