FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n02 mx48-2.3420000000cd9e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n02 mx48-2.3420000000cd9e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15936244
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTGCGGAAACCCGGAAGGCACAAGGAA186740.11717943073662777No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC168020.105432622643077Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG58600.033.5519754
CATGCGT59150.032.5969625
CGCGTGT47950.030.5054533
GCGTGTA56900.026.2913974
CGCGTAT58600.022.85411
CGTGTAC71300.021.3140645
CTGACAT113500.021.130421
GCGTATT66750.020.4904332
TACCCCG76200.020.0093179
ATGCGTG108550.019.7719256
GCGCGTG100600.019.071872
GTGTACC81150.019.0166996
CCGTATC51900.018.9966848-49
TCCGTAC67800.018.842065
TAAGTCG72700.018.8165639
TGTACCC83550.018.810967
GTACCCC81350.018.6209628
TGCGTGC117450.018.5562027
GCCGTAT48050.017.8488748-49
GCCAGTA71200.017.6758883
Produced by FastQC (version 0.11.2)