##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l05n02 mx48-2.3420000000cd9e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15936244 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.36136770998235 33.0 33.0 33.0 33.0 33.0 2 32.33594126696353 33.0 33.0 33.0 33.0 33.0 3 32.32999632786747 33.0 33.0 33.0 33.0 33.0 4 36.156844737066024 37.0 37.0 37.0 37.0 37.0 5 36.15246202304633 37.0 37.0 37.0 37.0 37.0 6 36.14484504629824 37.0 37.0 37.0 37.0 37.0 7 36.19524713602528 37.0 37.0 37.0 37.0 37.0 8 36.175247442245485 37.0 37.0 37.0 37.0 37.0 9 36.16251025021956 37.0 37.0 37.0 37.0 37.0 10-11 36.15137443929699 37.0 37.0 37.0 37.0 37.0 12-13 36.14433513944691 37.0 37.0 37.0 37.0 37.0 14-15 38.34395614173579 40.0 37.0 40.0 37.0 40.0 16-17 38.29654151881711 40.0 37.0 40.0 37.0 40.0 18-19 38.297893876373884 40.0 37.0 40.0 37.0 40.0 20-21 38.266515183878965 40.0 37.0 40.0 37.0 40.0 22-23 38.24738476644811 40.0 37.0 40.0 37.0 40.0 24-25 38.19489808890978 40.0 37.0 40.0 37.0 40.0 26-27 38.14831051156094 40.0 37.0 40.0 37.0 40.0 28-29 38.0930129144609 40.0 37.0 40.0 37.0 40.0 30-31 38.04407283799119 40.0 37.0 40.0 35.0 40.0 32-33 37.96085997428252 40.0 37.0 40.0 33.0 40.0 34-35 37.89083764028713 40.0 37.0 40.0 33.0 40.0 36-37 37.815385890175875 40.0 37.0 40.0 33.0 40.0 38-39 37.716072934124256 40.0 37.0 40.0 33.0 40.0 40-41 37.608507061011366 40.0 37.0 40.0 33.0 40.0 42-43 37.50430098208838 40.0 37.0 40.0 33.0 40.0 44-45 37.38755844852777 40.0 37.0 40.0 33.0 40.0 46-47 37.23643717428021 40.0 37.0 40.0 33.0 40.0 48-49 37.107456813537745 40.0 37.0 40.0 33.0 40.0 50-51 35.63542400580714 38.5 35.0 38.5 30.0 38.5 52-53 35.977764679054864 37.0 37.0 38.5 33.0 40.0 54-55 36.69182123466483 37.0 37.0 40.0 33.0 40.0 56-57 36.65636927998843 37.0 37.0 40.0 33.0 40.0 58-59 36.52291327241225 37.0 37.0 40.0 33.0 40.0 60-61 36.34573259545976 37.0 37.0 40.0 33.0 40.0 62-63 36.15417525610175 37.0 37.0 40.0 33.0 40.0 64-65 35.970961005617134 37.0 37.0 40.0 33.0 40.0 66-67 35.75025956555384 37.0 37.0 38.5 33.0 40.0 68-69 35.53912760120892 37.0 37.0 37.0 33.0 40.0 70-71 35.34227375032661 37.0 37.0 37.0 33.0 40.0 72-73 35.1368004280055 37.0 35.0 37.0 33.0 40.0 74-75 34.93754337596739 37.0 33.0 37.0 30.0 38.5 76-77 34.747394178954586 37.0 33.0 37.0 27.0 37.0 78-79 34.56600761760425 37.0 33.0 37.0 27.0 37.0 80-81 34.4349217732861 37.0 33.0 37.0 27.0 37.0 82-83 34.32811081456835 37.0 33.0 37.0 27.0 37.0 84-85 34.23052618295754 37.0 33.0 37.0 27.0 37.0 86-87 34.138589180737945 37.0 33.0 37.0 27.0 37.0 88-89 34.05653957733077 37.0 33.0 37.0 27.0 37.0 90-91 33.95529818695045 37.0 33.0 37.0 27.0 37.0 92-93 33.89021751298487 37.0 33.0 37.0 27.0 37.0 94-95 33.819082934473144 37.0 33.0 37.0 27.0 37.0 96-97 33.7340831691583 37.0 33.0 37.0 27.0 37.0 98-99 33.65440523501021 37.0 33.0 37.0 27.0 37.0 100-101 31.90842098677706 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.09447303166797383 2306 2 0.25003320617630465 2306 3 0.27368176798331945 2306 4 0.37932807505732313 2306 5 0.4133162335120346 2306 6 0.39184452064055364 2306 7 0.3824278302575266 2306 8 0.40732767811072534 2306 9 0.45738750428051134 2306 10-11 0.41684853962129154 2306 12-13 0.4090422948740198 2306 14-15 0.6873008183143341 2306 16-17 0.6912474471810626 2306 18-19 0.6948052834552456 2306 20-21 0.707119335787155 2306 22-23 0.6585925185743378 2306 24-25 0.6614180233161449 2306 26-27 0.7149311630671065 2306 28-29 0.7016318998363715 2306 30-31 0.7375646841923924 2306 32-33 0.768705824798289 2306 34-35 0.7436534430158801 2306 36-37 0.7181567301800413 2306 38-39 0.717323587990272 2306 40-41 0.780000298352931 2306 42-43 0.8045395904528831 2306 44-45 0.8054082847176787 2306 46-47 0.7892550746122708 2306 48-49 0.7985581446584717 2306 50-51 0.663546806795857 2306 52-53 0.7225701196118095 2306 54-55 0.7684904087068745 2306 56-57 0.783495302083864 2306 58-59 0.7809221309153571 2306 60-61 0.7314713463414293 2306 62-63 0.7318659269709613 2306 64-65 0.7689203891557739 2306 66-67 0.7960635535752161 2306 68-69 0.7976121233490332 2306 70-71 0.7395947582716786 2306 72-73 0.7640485735567282 2306 74-75 0.747236585190322 2306 76-77 0.79936833507697 2306 78-79 0.8205720667463794 2306 80-81 0.6760969950548912 2306 82-83 0.7607967672203912 2306 84-85 0.794878461675907 2306 86-87 0.7949867179204588 2306 88-89 0.7493714894647496 2306 90-91 0.9039438562815718 2306 92-93 0.8796015918690401 2306 94-95 0.8548903680789479 2306 96-97 0.8395551004859598 2306 98-99 0.7846981355070284 2306 100-101 0.4633064186414444 2307 1 0.04563785555995281 2307 2 0.1642368854918601 2307 3 0.18683031767733382 2307 4 0.2548570591752295 2307 5 0.31647070009614 2307 6 0.2847458044406892 2307 7 0.20794613764979175 2307 8 0.27444124154009586 2307 9 0.25611428264779335 2307 10-11 0.271709460290964 2307 12-13 0.31509859137228347 2307 14-15 0.4725531809450416 2307 16-17 0.5400305116350879 2307 18-19 0.4881119194085599 2307 20-21 0.5288552142213945 2307 22-23 0.5150800978198049 2307 24-25 0.4994304815914674 2307 26-27 0.5149757607421463 2307 28-29 0.5047987030970305 2307 30-31 0.4922385166084666 2307 32-33 0.4416316915873253 2307 34-35 0.5180580952394678 2307 36-37 0.5596222939582987 2307 38-39 0.48745688695901634 2307 40-41 0.5286311679000377 2307 42-43 0.4959778681742577 2307 44-45 0.4994271230977887 2307 46-47 0.5362097964555801 2307 48-49 0.513754587570304 2307 50-51 0.42749037966239456 2307 52-53 0.4028843885890794 2307 54-55 0.5221425855409549 2307 56-57 0.5025605306895571 2307 58-59 0.5709088797924835 2307 60-61 0.5711440059874491 2307 62-63 0.5416011068507132 2307 64-65 0.5551898891589175 2307 66-67 0.5716235267173957 2307 68-69 0.5896777937570405 2307 70-71 0.5316493246885727 2307 72-73 0.5391163614334715 2307 74-75 0.4785936618458706 2307 76-77 0.5214014380340046 2307 78-79 0.5471935293811754 2307 80-81 0.566481277618216 2307 82-83 0.5845158695425212 2307 84-85 0.5368834063651633 2307 86-87 0.6085098222761687 2307 88-89 0.5911370034248336 2307 90-91 0.479648785373179 2307 92-93 0.42571882428102725 2307 94-95 0.5154191567351774 2307 96-97 0.4538313216204912 2307 98-99 0.39205846095169505 2307 100-101 0.3496224070921201 2201 1 0.21664296586902054 2201 2 0.265696501326552 2201 3 0.3081378776443131 2201 4 0.34735165215688113 2201 5 0.3598671439243475 2201 6 0.38724096408755315 2201 7 0.36494252552770234 2201 8 0.40891599449829386 2201 9 0.4136302751408536 2201 10-11 0.387927502953481 2201 12-13 0.41213257295797945 2201 14-15 0.6325962053699996 2201 16-17 0.6763768818494853 2201 18-19 0.6616173504417233 2201 20-21 0.6684271523701995 2201 22-23 0.6068702564249762 2201 24-25 0.6590035320050802 2201 26-27 0.6289993765675774 2201 28-29 0.6907537899284648 2201 30-31 0.6469591854166694 2201 32-33 0.6953358809706316 2201 34-35 0.7132657085235365 2201 36-37 0.6872927037752135 2201 38-39 0.693593344576783 2201 40-41 0.7107283811079341 2201 42-43 0.6871063134829569 2201 44-45 0.5872928828473292 2201 46-47 0.6823720931644317 2201 48-49 0.6732722353998852 2201 50-51 0.6446583554521013 2201 52-53 0.7058024195180437 2201 54-55 0.7117050757586085 2201 56-57 0.6797211630036699 2201 58-59 0.691856259766368 2201 60-61 0.7159832131533008 2201 62-63 0.7734321664662076 2201 64-65 0.7750803647806279 2201 66-67 0.749275872764052 2201 68-69 0.7533981172997031 2201 70-71 0.7577512936711273 2201 72-73 0.7652574703404156 2201 74-75 0.7762377670214633 2201 76-77 0.8355149197999125 2201 78-79 0.8217537371387635 2201 80-81 0.815776011149687 2201 82-83 0.7730746250361733 2201 84-85 0.8305058527115534 2201 86-87 0.8300323147773341 2201 88-89 0.8123808820186014 2201 90-91 0.864943970551586 2201 92-93 0.8568677158817621 2201 94-95 0.8828758052664512 2201 96-97 0.8767197722040194 2201 98-99 0.8100415288266092 2201 100-101 0.7085632992759514 1101 1 0.22096116007212174 1101 2 0.20197411642390506 1101 3 0.2297024262486005 1101 4 0.2703810619606841 1101 5 0.32597452298490026 1101 6 0.2683521242341058 1101 7 0.35734095627774565 1101 8 0.3445267604263975 1101 9 0.346374684051618 1101 10-11 0.3488006276930875 1101 12-13 0.3621298785300482 1101 14-15 0.5680220673628682 1101 16-17 0.572908054527673 1101 18-19 0.543720105447953 1101 20-21 0.5565790399011092 1101 22-23 0.5692562012905142 1101 24-25 0.5684298964284054 1101 26-27 0.5934553832907454 1101 28-29 0.5959996274532955 1101 30-31 0.5950551400271422 1101 32-33 0.6057881511935008 1101 34-35 0.6062610202945606 1101 36-37 0.616246996104806 1101 38-39 0.6256623615636272 1101 40-41 0.6411919350277486 1101 42-43 0.5807371585527434 1101 44-45 0.6038913984501093 1101 46-47 0.6600557027342973 1101 48-49 0.6338699347706296 1101 50-51 0.6740518905873003 1101 52-53 0.7147885163077206 1101 54-55 0.6347343758944746 1101 56-57 0.6295178821245102 1101 58-59 0.6359520609929916 1101 60-61 0.7065698084556331 1101 62-63 0.7097549042500404 1101 64-65 0.7018839968770365 1101 66-67 0.7046981754648911 1101 68-69 0.6847349835291325 1101 70-71 0.7314425665343904 1101 72-73 0.7010662738854592 1101 74-75 0.700865220288641 1101 76-77 0.7250034440139004 1101 78-79 0.7653030705637605 1101 80-81 0.7559212780646405 1101 82-83 0.678511848249876 1101 84-85 0.6909523842756329 1101 86-87 0.7357712231600146 1101 88-89 0.7029320848282481 1101 90-91 0.7463501563757475 1101 92-93 0.7850206342913495 1101 94-95 0.7214338291196825 1101 96-97 0.7692132774982028 1101 98-99 0.766025259549302 1101 100-101 0.6693843960302743 2304 1 0.10998464586979395 2304 2 0.20352165390134758 2304 3 0.2035885951431169 2304 4 0.29334064905790314 2304 5 0.3178177987577513 2304 6 0.32674315163662015 2304 7 0.31738569491601254 2304 8 0.3345762062295847 2304 9 0.3721762476013524 2304 10-11 0.345743592477298 2304 12-13 0.3400375172904049 2304 14-15 0.5344570079796682 2304 16-17 0.5958374225214342 2304 18-19 0.5083905112268852 2304 20-21 0.519268659632445 2304 22-23 0.5470686256425097 2304 24-25 0.598695731932466 2304 26-27 0.5717204875113282 2304 28-29 0.6329418381371568 2304 30-31 0.5881815544272797 2304 32-33 0.5909018519387885 2304 34-35 0.5711132288302068 2304 36-37 0.6108210823355336 2304 38-39 0.6264485463895042 2304 40-41 0.5608479684797345 2304 42-43 0.5868703464810707 2304 44-45 0.6524255623825965 2304 46-47 0.6829713959474191 2304 48-49 0.6650038433968462 2304 50-51 0.578809292377862 2304 52-53 0.657283291823255 2304 54-55 0.6467553065234739 2304 56-57 0.6643285982655911 2304 58-59 0.6642902789170506 2304 60-61 0.631553507852125 2304 62-63 0.6730715625330888 2304 64-65 0.6512873747751868 2304 66-67 0.6851910276689139 2304 68-69 0.6917657523992133 2304 70-71 0.6835124638959869 2304 72-73 0.6704774178874828 2304 74-75 0.6833583142454884 2304 76-77 0.7263343049059827 2304 78-79 0.7626040517045212 2304 80-81 0.7139289694976512 2304 82-83 0.7252930636005104 2304 84-85 0.6868222448819878 2304 86-87 0.6235335608560248 2304 88-89 0.7421061089708729 2304 90-91 0.7209871306980986 2304 92-93 0.7423735331558632 2304 94-95 0.6663441071475731 2304 96-97 0.7139091452863298 2304 98-99 0.614591955652422 2304 100-101 0.7028220519243362 2202 1 0.1531300077915958 2202 2 0.22684230341317857 2202 3 0.26493994672474486 2202 4 0.3531859615728692 2202 5 0.365907625344164 2202 6 0.334935368281549 2202 7 0.3745061131232248 2202 8 0.3872492250860802 2202 9 0.39900405998228194 2202 10-11 0.3857589234971499 2202 12-13 0.39671602367860714 2202 14-15 0.6338029852140252 2202 16-17 0.652411668288515 2202 18-19 0.638845772590777 2202 20-21 0.6471790051844337 2202 22-23 0.6394401316019085 2202 24-25 0.6681865233578748 2202 26-27 0.6345875344121268 2202 28-29 0.6666604666581151 2202 30-31 0.7302326932828578 2202 32-33 0.6569224350930298 2202 34-35 0.6513500727878139 2202 36-37 0.7144586173190817 2202 38-39 0.7404552261545021 2202 40-41 0.6903169393155935 2202 42-43 0.7037956024080074 2202 44-45 0.6988711018135234 2202 46-47 0.7357700544577739 2202 48-49 0.6713890291569058 2202 50-51 0.669092416989912 2202 52-53 0.6965627782852195 2202 54-55 0.778875669797678 2202 56-57 0.8057693752656618 2202 58-59 0.8130210594431588 2202 60-61 0.7846106295633106 2202 62-63 0.8204190774135398 2202 64-65 0.8066141717324129 2202 66-67 0.8578863100170651 2202 68-69 0.869642309288686 2202 70-71 0.8380675902532104 2202 72-73 0.8519095272798438 2202 74-75 0.8471331849066388 2202 76-77 0.8544703093062438 2202 78-79 0.8814167163550195 2202 80-81 0.8665411898379674 2202 82-83 0.8955996865385956 2202 84-85 0.868265322192741 2202 86-87 0.9440402692683918 2202 88-89 0.9463479385144282 2202 90-91 0.9001305213990989 2202 92-93 0.9158514801809687 2202 94-95 0.9315942035643516 2202 96-97 0.9348160784591855 2202 98-99 0.9958409355612758 2202 100-101 1.0688880732773 1102 1 0.05623822439888926 1102 2 0.09285645864930814 1102 3 0.11142857862099476 1102 4 0.12982769939303296 1102 5 0.13882364691889393 1102 6 0.111670369967527 1102 7 0.13320136213945233 1102 8 0.17899648990153594 1102 9 0.18927203693395 1102 10-11 0.16085582121309727 1102 12-13 0.1756562230978247 1102 14-15 0.25644233452798204 1102 16-17 0.29965224626946707 1102 18-19 0.26242736460331884 1102 20-21 0.2883490991094817 1102 22-23 0.34155361438570964 1102 24-25 0.30860285032022006 1102 26-27 0.3239573821069115 1102 28-29 0.336640110899161 1102 30-31 0.38543657349481464 1102 32-33 0.387934630497746 1102 34-35 0.40259810471848567 1102 36-37 0.36754725901353424 1102 38-39 0.36482136106702256 1102 40-41 0.345290581869385 1102 42-43 0.384688143549063 1102 44-45 0.39183507427183173 1102 46-47 0.43595332117968866 1102 48-49 0.4089733956081858 1102 50-51 0.3280487858563532 1102 52-53 0.3569842455753971 1102 54-55 0.4830547075035625 1102 56-57 0.49707446398783617 1102 58-59 0.468918236265111 1102 60-61 0.4620196963770624 1102 62-63 0.40806499384196115 1102 64-65 0.4483844944669997 1102 66-67 0.4839788225857049 1102 68-69 0.4481922127745648 1102 70-71 0.46403375450723416 1102 72-73 0.4703416427913396 1102 74-75 0.4558139421626777 1102 76-77 0.5241153680056172 1102 78-79 0.4847997369099204 1102 80-81 0.4490333226520349 1102 82-83 0.4212144434753 1102 84-85 0.43927556377448695 1102 86-87 0.502112489603256 1102 88-89 0.54735631026508 1102 90-91 0.5261905472020558 1102 92-93 0.5268288617138879 1102 94-95 0.46354708642659403 1102 96-97 0.5463157433328831 1102 98-99 0.5336391214102107 1102 100-101 0.5026929376943698 2305 1 0.14376283380207866 2305 2 0.13975136922741171 2305 3 0.1466193423755584 2305 4 0.18754701140439778 2305 5 0.18660634088267614 2305 6 0.21692474530456707 2305 7 0.18278636902132916 2305 8 0.2195979613999981 2305 9 0.19853790690633843 2305 10-11 0.1977064475309689 2305 12-13 0.20954927985191318 2305 14-15 0.3146186589389117 2305 16-17 0.3595607704852952 2305 18-19 0.3416561306416739 2305 20-21 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-1.133217214877483 1213 94-95 -1.0206425890419624 1213 96-97 -1.1788919643481819 1213 98-99 -1.15680370528478 1213 100-101 -0.9783588371218883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15574.0 3 1145.0 4 633.0 5 587.0 6 623.0 7 1436.0 8 2699.0 9 6099.0 10 16035.0 11 22007.0 12 20118.0 13 20312.0 14 21527.0 15 23555.0 16 26382.0 17 30363.0 18 34278.0 19 38534.0 20 43182.0 21 48382.0 22 54279.0 23 61157.0 24 70023.0 25 80633.0 26 93219.0 27 113788.0 28 131296.0 29 154738.0 30 191827.0 31 245144.0 32 321471.0 33 435835.0 34 612875.0 35 950035.0 36 1986504.0 37 5304885.0 38 4749179.0 39 5885.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.288394690173742 13.097119053266335 15.744555323256328 49.86993093330359 2 23.341624102971057 18.88382561139191 36.25037253180404 21.524177753832994 3 25.693148052022785 23.399899526599036 24.95717442211304 25.949777999265137 4 27.575132615795678 28.091195788864194 18.446713932876353 25.88695766246377 5 28.74144932751925 29.915962838580747 21.529706793516983 19.812881040383022 6 21.908168142300077 33.46168169438934 22.252052627267414 22.378097536043164 7 19.619123461524353 15.977052971961283 41.32555172529908 23.07827184121529 8 21.73566618387579 21.03418391850917 27.384627272564504 29.84552262505053 9 22.42725979592456 20.82933453630717 30.31010981114915 26.43329585661912 10-11 24.955957977316583 29.28682308974391 21.009853045228745 24.747365887710767 12-13 23.081875892445762 23.30347711835108 28.003934621607705 25.61071236759545 14-15 23.770217672367476 25.158576078624627 26.494795321262586 24.57641092774531 16-17 24.662791498982482 25.154668953400854 25.58526048184005 24.597279065776615 18-19 24.595818570865205 25.4031681221996 25.77072361143723 24.230289695497966 20-21 24.34566136464334 25.49374865685267 25.87844427916194 24.282145699342045 22-23 24.39958385480053 25.373607961848478 25.69910593009423 24.527702253256763 24-25 24.480830510323447 25.552730171863868 25.48103435015309 24.48540496765959 26-27 24.14687092294598 25.647112119431366 25.58308735837267 24.62292959924999 28-29 24.4348859470432 25.545527451668658 25.290378550615383 24.729208050672764 30-31 24.62687115614116 25.16178825463785 25.463957778490087 24.747382810730905 32-33 24.492215697196603 25.497737346236654 25.270052393669346 24.7399945628974 34-35 24.250325773220748 25.66538204533969 25.06033491883997 25.023957262599588 36-37 24.19291944786831 25.46423571100766 25.430113794486857 24.91273104663717 38-39 24.28503712074049 25.320896668395005 25.561721540786568 24.832344670077937 40-41 24.831677109189982 25.095847665029915 25.322788259481705 24.7496869662984 42-43 24.544476703050385 25.24844725960894 25.736139446656132 24.470936590684538 44-45 24.610930247317363 25.140517657940592 25.60426834364163 24.64428375110042 46-47 24.560843545420315 25.155053422041895 25.452354763763154 24.831748268774643 48-49 24.23860834233162 25.50342848636124 25.31549228728875 24.942470884018388 50-51 24.122287333760745 25.305422318902465 25.89789639822041 24.67439394911638 52-53 24.467309987130868 25.427820882545383 25.544189620073205 24.560679510250544 54-55 24.37037404669592 25.460138154685374 25.42640519196754 24.743082606651164 56-57 23.972949673297993 25.34489329878783 25.90564311115821 24.77651391675596 58-59 24.5332087532678 25.29108931616104 25.519500412410235 24.656201518160923 60-61 24.542957007575822 25.122677295269824 25.54875706891015 24.785608628244205 62-63 24.21165144099759 25.290658516298848 25.736444046722195 24.76124599598137 64-65 24.37440384956248 25.430815327274907 25.27197275408553 24.922808069077078 66-67 24.27759099626759 25.428508522604997 25.238790213781275 25.055110267346137 68-69 24.343709149105113 25.374456981145023 25.434468488979157 24.847365380770707 70-71 24.457628457616522 25.405112091945846 25.193538259491426 24.9437211909462 72-73 24.370896384635802 25.496214432349245 25.515335697894937 24.617553485120013 74-75 24.77188888953839 25.267552504666813 25.355275822312212 24.605282783482586 76-77 24.97536688018147 25.339367212719548 25.15164663711436 24.53361926998462 78-79 24.5092960863591 25.354115408196076 25.23809917428361 24.898489331161212 80-81 24.670012000077797 25.42932775527093 25.110025326445257 24.79063491820601 82-83 24.607350567275482 25.40226885060334 25.071278567032202 24.919102015088978 84-85 24.615747993053123 25.275848081753672 24.988574302628745 25.119829622564456 86-87 24.49694620227466 25.70462087743433 25.133085604067134 24.665347316223873 88-89 24.67128579033881 25.59477123443812 25.158135996737286 24.57580697848579 90-91 24.865486534420295 25.340392975033136 25.092478547613073 24.701641942933495 92-93 24.706138859907796 25.55039064946703 25.43554825651594 24.307922234109235 94-95 24.531063158885367 25.48272346550693 25.230731990911696 24.75548138469601 96-97 24.354499262376866 25.66658982012538 25.140667965550172 24.83824295194758 98-99 24.609811469541683 25.244852308336885 25.387937849026425 24.757398373095004 100-101 24.64315796955813 25.497568619819035 25.235825047420057 24.623448363202787 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13962.0 1 7430.0 2 734.0 3 502.5 4 411.5 5 376.0 6 376.5 7 406.5 8 421.5 9 438.5 10 472.5 11 532.0 12 606.0 13 701.0 14 830.0 15 955.5 16 1153.5 17 1485.5 18 1833.5 19 2286.5 20 3017.0 21 4112.0 22 5752.0 23 8132.0 24 11221.5 25 15885.5 26 22240.0 27 30197.5 28 40815.0 29 54130.5 30 69477.0 31 87775.0 32 109468.0 33 134231.5 34 165391.0 35 208066.0 36 257067.5 37 320320.0 38 382846.0 39 433695.5 40 490302.0 41 540878.5 42 581301.5 43 619037.0 44 655908.5 45 658072.5 46 633879.5 47 638749.5 48 613005.0 49 571801.5 50 581887.0 51 556770.0 52 507807.0 53 477330.0 54 456470.5 55 462260.5 56 478305.0 57 479920.0 58 438737.0 59 361875.0 60 316587.0 61 285925.0 62 244532.5 63 219378.5 64 190548.5 65 170238.5 66 170758.0 67 174152.0 68 163126.0 69 140510.5 70 119933.0 71 107435.0 72 87142.0 73 66902.0 74 68295.5 75 61995.0 76 40663.0 77 25573.0 78 19939.0 79 21172.0 80 19366.5 81 13117.0 82 6038.5 83 1443.5 84 911.0 85 674.0 86 550.0 87 445.0 88 369.0 89 296.5 90 249.5 91 218.0 92 181.5 93 136.5 94 101.5 95 84.5 96 67.0 97 45.5 98 31.0 99 21.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12844306349727075 2 0.11382857842789054 3 0.11045890110618285 4 0.12291478468828665 5 0.12519888626203263 6 0.11362777828953924 7 0.11633858015728173 8 0.11532830446120178 9 0.11241670245510799 10-11 0.1000078814054303 12-13 0.11018593841811157 14-15 0.14471414970804913 16-17 0.1553722445514765 18-19 0.14432823694215524 20-21 0.1680634407957107 22-23 0.15065971630454453 24-25 0.1367197941999382 26-27 0.14350621137577962 28-29 0.1319476534119332 30-31 0.1016989950706076 32-33 0.10040006917564767 34-35 0.12475022345290396 36-37 0.1241666480508205 38-39 0.1327288914502062 40-41 0.16951610429659586 42-43 0.13679509425181996 44-45 0.13725944457175732 46-47 0.15759673358414944 48-49 0.18521302761177602 50-51 0.15634173271945384 52-53 0.11488905415855831 54-55 0.11658330532589736 56-57 0.11241670245510799 58-59 0.11442156633645921 60-61 0.11651114277617736 62-63 0.10130680730039024 64-65 0.11274614018209059 66-67 0.13386466723275572 68-69 0.1263158370316117 70-71 0.11543811703686264 72-73 0.12180097142086929 74-75 0.16668293984454555 76-77 0.17752928481767724 78-79 0.1737831072365609 80-81 0.1470264888012508 82-83 0.12876308871776812 84-85 0.13466473028399917 86-87 0.1259926743089526 88-89 0.12971061437061332 90-91 0.14237043559323012 92-93 0.11782575618194602 94-95 0.11697863059827648 96-97 0.12073422068587805 98-99 0.11823676896513381 100-101 0.11050596363860894 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5936244E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.74529191484039 #Duplication Level Percentage of deduplicated Percentage of total 1 77.84969566923579 38.7265583654762 2 13.312524604309353 13.244708451297276 3 3.9158061285497996 5.843787568398923 4 1.7578668350179825 3.497823954215444 5 0.9440745424689805 2.3481631852244407 6 0.48790280559068866 1.4562520494107056 7 0.32385652269251974 1.1277236081905169 8 0.24211399016446497 0.9635224893918484 9 0.18901773759378188 0.8462468280316842 >10 0.8178273201018351 7.206660243756385 >50 0.06558233498249656 2.295520960779351 >100 0.07061450601609848 7.552539729379442 >500 0.013499854883770208 4.68264305568945 >1k 0.00917533951288086 8.36428381797585 >5k 3.4038019491159803E-4 1.160486707621482 >10k+ 1.0142868465136875E-4 0.6830789851609305 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGACATGCGTGCGAGTCGACGGGTGCGGAAACCCGGAAGGCACAAGGAA 18674 0.11717943073662777 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 16802 0.105432622643077 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.11209040223028714 0.0 1.2550008646955958E-5 2 0.11231002738160886 0.0 1.2550008646955958E-5 3 0.11252337752860711 6.275004323477979E-6 1.2550008646955958E-5 4 0.11335795310362969 6.275004323477979E-6 1.8825012970433938E-5 5 0.11350855320739316 1.8825012970433938E-5 1.8825012970433938E-5 6 0.11417370366568183 5.020003458782383E-5 1.8825012970433938E-5 7 0.11438705381268008 6.275004323477979E-5 1.8825012970433938E-5 8 0.11465687899858963 6.902504755825777E-5 2.5100017293911915E-5 9 0.11514632933582092 6.902504755825777E-5 3.7650025940867876E-5 10-11 0.11598090491084348 8.78500605286917E-5 3.7650025940867876E-5 12-13 0.11689705554207128 1.1295007782260362E-4 4.392503026434585E-5 14-15 0.11806734384839991 1.3177509079303758E-4 6.588754539651879E-5 16-17 0.11907761954447987 1.757001210573834E-4 6.902504755825777E-5 18-19 0.12005024521461896 1.8825012970433936E-4 7.216254971999675E-5 20-21 0.12133034609660846 2.1648764915999026E-4 7.530005188173575E-5 22-23 0.12226218423864493 2.5100017293911916E-4 7.530005188173575E-5 24-25 0.12317205986554924 2.729626880712921E-4 8.157505620521372E-5 26-27 0.12398467292543965 2.85512696718248E-4 8.78500605286917E-5 28-29 0.1247533609550657 3.01200207526943E-4 1.0040006917564766E-4 30-31 0.1253965488982222 3.1061271401216E-4 1.0040006917564766E-4 32-33 0.1260177743262465 3.200252204973769E-4 1.0667507349912564E-4 34-35 0.12663899975427084 3.231627226591159E-4 1.1295007782260362E-4 36-37 0.12729473770607427 3.482627399530278E-4 1.192250821460816E-4 38-39 0.12802263820759774 3.576752464382448E-4 1.223625843078206E-4 40-41 0.1287599512156064 3.639502507617228E-4 1.443250994399935E-4 42-43 0.129437651682542 3.6708775292346174E-4 1.537376059252105E-4 44-45 0.1300965271365072 3.827752637321567E-4 1.7256261889564441E-4 46-47 0.1309562027288237 3.953252723791127E-4 1.757001210573834E-4 48-49 0.13182215332546365 4.0787528102606866E-4 1.819751253808614E-4 50-51 0.13286694154532272 4.423878048051975E-4 1.8511262754260038E-4 52-53 0.13403722985165137 4.643503199373704E-4 1.9138763186607834E-4 54-55 0.13514476811474524 4.8317533290780437E-4 2.0080013835129532E-4 56-57 0.13614249380217824 4.957253415547604E-4 2.1962515132172925E-4 58-59 0.13718414451987557 5.051378480399773E-4 2.1962515132172925E-4 60-61 0.1384579703975416 5.208253588486723E-4 2.1962515132172925E-4 62-63 0.14010515903245457 5.333753674956282E-4 2.2590015564520724E-4 64-65 0.14177744768466144 5.427878739808452E-4 2.2590015564520724E-4 66-67 0.14350621137577962 5.522003804660622E-4 2.2590015564520724E-4 68-69 0.14531655012310302 5.553378826278011E-4 2.2590015564520724E-4 70-71 0.14732768900877774 5.86712904245191E-4 2.2903765780694623E-4 72-73 0.1498031782143898 6.02400415053886E-4 2.447251686156412E-4 74-75 0.15240103000430966 6.118129215391029E-4 2.5100017293911916E-4 76-77 0.1551557569023165 6.337754366712759E-4 2.5727517726259713E-4 78-79 0.15856935925428853 6.431879431564928E-4 2.635501815860751E-4 80-81 0.16286146221154746 6.620129561269268E-4 2.666876837478141E-4 82-83 0.1681010908216516 6.651504582886658E-4 2.729626880712921E-4 84-85 0.1750004580753156 6.777004669356217E-4 2.792376923947701E-4 86-87 0.18351877644443698 6.839754712590997E-4 2.8237519455650905E-4 88-89 0.19363408341388347 7.122129907147505E-4 2.8237519455650905E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATGCG 5860 0.0 33.551975 4 CATGCGT 5915 0.0 32.596962 5 CGCGTGT 4795 0.0 30.505453 3 GCGTGTA 5690 0.0 26.291397 4 CGCGTAT 5860 0.0 22.8541 1 CGTGTAC 7130 0.0 21.314064 5 CTGACAT 11350 0.0 21.13042 1 GCGTATT 6675 0.0 20.490433 2 TACCCCG 7620 0.0 20.009317 9 ATGCGTG 10855 0.0 19.771925 6 GCGCGTG 10060 0.0 19.07187 2 GTGTACC 8115 0.0 19.016699 6 CCGTATC 5190 0.0 18.99668 48-49 TCCGTAC 6780 0.0 18.84206 5 TAAGTCG 7270 0.0 18.816563 9 TGTACCC 8355 0.0 18.81096 7 GTACCCC 8135 0.0 18.620962 8 TGCGTGC 11745 0.0 18.556202 7 GCCGTAT 4805 0.0 17.84887 48-49 GCCAGTA 7120 0.0 17.675888 3 >>END_MODULE