FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n02 mx12-1.3420000000cc4f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n02 mx12-1.3420000000cc4f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19582760
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC242770.12397128903178102Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT129350.019.0952479
GCCGTAT75300.016.59485848-49
AAGAGCG156700.016.0311268
GAGTGTA80500.015.10278228-29
CGAATTA61150.014.72255914-15
CCGTATC89550.014.6969448-49
AGTGTAG91550.013.9542628-29
ATCGGAA182100.013.7967713
CGCCGTA95400.013.72246546-47
TCGTGTA96100.013.66917514-15
TGTAGGG95100.013.26328918-19
CGTGTAG95000.013.20233916-17
GTAGGGA104350.013.04365818-19
GTCGTGT97500.012.74200214-15
CGCGTAT18600.012.2565371
AGATCGG202350.012.0172821
GTGTAGG111900.012.01508716-17
GATCGGA212250.011.9488892
TCGGAAG211850.011.7023684
TTAAAAA153450.011.69758256-57
Produced by FastQC (version 0.11.2)