##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l05n02 mx0-1.3420000000ca34.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26242500 Sequences flagged as poor quality 0 Sequence length 101 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38220028579594 33.0 33.0 33.0 33.0 33.0 2 32.31992146327522 33.0 33.0 33.0 33.0 33.0 3 32.30257818424312 33.0 33.0 33.0 33.0 33.0 4 36.12507554539392 37.0 37.0 37.0 37.0 37.0 5 36.12072458797752 37.0 37.0 37.0 37.0 37.0 6 36.131226636181765 37.0 37.0 37.0 37.0 37.0 7 36.17065649233114 37.0 37.0 37.0 37.0 37.0 8 36.15425439649424 37.0 37.0 37.0 37.0 37.0 9 36.120452548347146 37.0 37.0 37.0 37.0 37.0 10-11 36.111071601409925 37.0 37.0 37.0 37.0 37.0 12-13 36.10664285033819 37.0 37.0 37.0 37.0 37.0 14-15 38.302505515861675 40.0 37.0 40.0 37.0 40.0 16-17 38.24480556349433 40.0 37.0 40.0 37.0 40.0 18-19 38.24337665999809 40.0 37.0 40.0 37.0 40.0 20-21 38.20761958654853 40.0 37.0 40.0 37.0 40.0 22-23 38.230652586453274 40.0 37.0 40.0 37.0 40.0 24-25 38.15560352481661 40.0 37.0 40.0 37.0 40.0 26-27 38.097912908450034 40.0 37.0 40.0 37.0 40.0 28-29 38.024891816709534 40.0 37.0 40.0 37.0 40.0 30-31 37.97750907878441 40.0 37.0 40.0 35.0 40.0 32-33 37.87901362293989 40.0 37.0 40.0 33.0 40.0 34-35 37.792352253024674 40.0 37.0 40.0 33.0 40.0 36-37 37.703501724302185 40.0 37.0 40.0 33.0 40.0 38-39 37.59288733923978 40.0 37.0 40.0 33.0 40.0 40-41 37.467562560731636 40.0 37.0 40.0 33.0 40.0 42-43 37.32728638658664 40.0 37.0 40.0 33.0 40.0 44-45 37.19670069543679 40.0 37.0 40.0 33.0 40.0 46-47 37.000020024768986 40.0 37.0 40.0 33.0 40.0 48-49 36.8367024864247 38.5 37.0 40.0 33.0 40.0 50-51 35.295765247213495 37.0 35.0 38.5 30.0 38.5 52-53 35.63915450128608 37.0 35.0 38.5 33.0 40.0 54-55 36.32964193579117 37.0 37.0 40.0 33.0 40.0 56-57 36.280148042297796 37.0 37.0 40.0 33.0 40.0 58-59 36.12678965418691 37.0 37.0 40.0 33.0 40.0 60-61 35.965484062112985 37.0 37.0 40.0 33.0 40.0 62-63 35.78500472515957 37.0 37.0 38.5 33.0 40.0 64-65 35.59756941983424 37.0 37.0 37.0 33.0 40.0 66-67 35.38576149376013 37.0 37.0 37.0 33.0 40.0 68-69 35.17446792416881 37.0 33.0 37.0 33.0 40.0 70-71 34.99977199199772 37.0 33.0 37.0 30.0 40.0 72-73 34.818763818233776 37.0 33.0 37.0 27.0 37.0 74-75 34.64595560636373 37.0 33.0 37.0 27.0 37.0 76-77 34.49280636372296 37.0 33.0 37.0 27.0 37.0 78-79 34.34200102886539 37.0 33.0 37.0 27.0 37.0 80-81 34.22535339620844 37.0 33.0 37.0 27.0 37.0 82-83 34.13266628560541 37.0 33.0 37.0 27.0 37.0 84-85 34.045241002191105 37.0 33.0 37.0 27.0 37.0 86-87 33.96591866247499 37.0 33.0 37.0 27.0 37.0 88-89 33.89270938363342 37.0 33.0 37.0 27.0 37.0 90-91 33.789927045822616 37.0 33.0 37.0 27.0 37.0 92-93 33.72622728398591 37.0 33.0 37.0 27.0 37.0 94-95 33.65755831189864 37.0 33.0 37.0 27.0 37.0 96-97 33.56855271029818 37.0 33.0 37.0 27.0 37.0 98-99 33.4882026102696 37.0 33.0 37.0 27.0 37.0 100-101 31.676244965228157 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12744145043541977 2306 2 0.2653856864786093 2306 3 0.2577298909033061 2306 4 0.36586339838928694 2306 5 0.37927902737936847 2306 6 0.36915306965918404 2306 7 0.38446097847336347 2306 8 0.3862144267276193 2306 9 0.4025959323286372 2306 10-11 0.39434411890403 2306 12-13 0.4056041820719969 2306 14-15 0.6684132707060542 2306 16-17 0.6617034349213071 2306 18-19 0.677270657960996 2306 20-21 0.704554105790379 2306 22-23 0.6381168490746916 2306 24-25 0.6505308816641246 2306 26-27 0.6990239349516045 2306 28-29 0.6522969596740751 2306 30-31 0.6834655751616125 2306 32-33 0.7268611450122577 2306 34-35 0.6973205505091329 2306 36-37 0.6617883887379605 2306 38-39 0.6915850211045793 2306 40-41 0.7336485926265297 2306 42-43 0.7622243780557199 2306 44-45 0.7763877212200114 2306 46-47 0.7480886452221469 2306 48-49 0.7355183655823581 2306 50-51 0.5960556215807387 2306 52-53 0.6815666216086029 2306 54-55 0.6991107036265305 2306 56-57 0.693478178906993 2306 58-59 0.7435641156315143 2306 60-61 0.6679045220619244 2306 62-63 0.6947976474873201 2306 64-65 0.7118104459007313 2306 66-67 0.7283710131517154 2306 68-69 0.7724060939910657 2306 70-71 0.7471243184106058 2306 72-73 0.7376253275779874 2306 74-75 0.7316560034828825 2306 76-77 0.7782542087006021 2306 78-79 0.7467863587027779 2306 80-81 0.6365175584130682 2306 82-83 0.7553447114485792 2306 84-85 0.7842118639778377 2306 86-87 0.7222496740432405 2306 88-89 0.7547532420684888 2306 90-91 0.7947965602066702 2306 92-93 0.7740289874087338 2306 94-95 0.6922195666436934 2306 96-97 0.7670142957972956 2306 98-99 0.694551779245252 2306 100-101 0.36021038574842024 2307 1 -0.0050236550816933345 2307 2 0.13540393469886425 2307 3 0.17331031190194324 2307 4 0.20855152119476372 2307 5 0.29328536476889866 2307 6 0.2585047936279352 2307 7 0.21647106243783298 2307 8 0.2200264628189572 2307 9 0.24111068188024376 2307 10-11 0.2440343009799335 2307 12-13 0.2550586700362487 2307 14-15 0.4450974918301185 2307 16-17 0.48981634650814954 2307 18-19 0.4313826987881484 2307 20-21 0.49065039760449736 2307 22-23 0.47640152616577325 2307 24-25 0.47931722020864953 2307 26-27 0.4739165156327303 2307 28-29 0.4521774655170532 2307 30-31 0.47120134486743126 2307 32-33 0.4360900244051038 2307 34-35 0.5008444186344008 2307 36-37 0.5073621061305715 2307 38-39 0.45469838476586943 2307 40-41 0.4947461205650612 2307 42-43 0.4497384800953341 2307 44-45 0.4523226433256511 2307 46-47 0.4772267671034598 2307 48-49 0.42054850700180424 2307 50-51 0.33644565918873326 2307 52-53 0.33071283227929626 2307 54-55 0.4583359129755422 2307 56-57 0.46468192368470795 2307 58-59 0.49628370648092357 2307 60-61 0.4903885584891938 2307 62-63 0.49935718239108695 2307 64-65 0.5503211123023348 2307 66-67 0.5835114977874554 2307 68-69 0.5729235765594751 2307 70-71 0.5212480850650607 2307 72-73 0.5284803896576307 2307 74-75 0.5105248589223734 2307 76-77 0.5151749316227949 2307 78-79 0.5089406878468736 2307 80-81 0.5226660059472579 2307 82-83 0.5066351997937417 2307 84-85 0.5153348599124428 2307 86-87 0.5530845827165365 2307 88-89 0.5481077935148164 2307 90-91 0.5006152480741193 2307 92-93 0.46844699341249196 2307 94-95 0.4731790321541425 2307 96-97 0.3904139618456668 2307 98-99 0.3348613452597533 2307 100-101 0.3324358571004389 2201 1 0.2113533365898661 2201 2 0.28476398674116865 2201 3 0.2785866920187132 2201 4 0.3267785772056797 2201 5 0.37160544647910143 2201 6 0.3653561534055143 2201 7 0.357790663312727 2201 8 0.39188391233919617 2201 9 0.39252685390319897 2201 10-11 0.3881057144886171 2201 12-13 0.37930669014896523 2201 14-15 0.6245121448926128 2201 16-17 0.6545512842846009 2201 18-19 0.6515648230199389 2201 20-21 0.6588130199825599 2201 22-23 0.6122115033469839 2201 24-25 0.6763186070003968 2201 26-27 0.617665215665653 2201 28-29 0.6609246902514059 2201 30-31 0.6347203536117192 2201 32-33 0.6430603933262375 2201 34-35 0.6573224674264253 2201 36-37 0.6481274071450898 2201 38-39 0.6364920931063764 2201 40-41 0.6887530634706209 2201 42-43 0.6663980909862062 2201 44-45 0.5806598154668379 2201 46-47 0.6825733232600797 2201 48-49 0.6632890897316273 2201 50-51 0.6317072824378869 2201 52-53 0.7227628778599495 2201 54-55 0.689532977558386 2201 56-57 0.6259540782106754 2201 58-59 0.6572563325109115 2201 60-61 0.6790659367736467 2201 62-63 0.7627823485779928 2201 64-65 0.7245767445933708 2201 66-67 0.7100835013416287 2201 68-69 0.7265867876520886 2201 70-71 0.7488798016293927 2201 72-73 0.7688942670099408 2201 74-75 0.7508255009628542 2201 76-77 0.7689625772459081 2201 78-79 0.8187823360524362 2201 80-81 0.7717161842881453 2201 82-83 0.7382076096511767 2201 84-85 0.7526277256635652 2201 86-87 0.8186471018070591 2201 88-89 0.7988434233007524 2201 90-91 0.7905351989391534 2201 92-93 0.7726352728982562 2201 94-95 0.7883731157133056 2201 96-97 0.7765915575152889 2201 98-99 0.7891763431143701 2201 100-101 0.6053995559565983 1101 1 0.15795251987475467 1101 2 0.16472834332126496 1101 3 0.18117113770563265 1101 4 0.21982298291960944 1101 5 0.24581741824483316 1101 6 0.20487248134219982 1101 7 0.2804839440407534 1101 8 0.2908088296888778 1101 9 0.27607897514715063 1101 10-11 0.2901065010753143 1101 12-13 0.2882626162206279 1101 14-15 0.5089488362408545 1101 16-17 0.5309376742648197 1101 18-19 0.46588353418987083 1101 20-21 0.5160742924983879 1101 22-23 0.48517332705954885 1101 24-25 0.5212815235897423 1101 26-27 0.5005665606548604 1101 28-29 0.5122962593764768 1101 30-31 0.5509708245486848 1101 32-33 0.5500365846356772 1101 34-35 0.55739529970203 1101 36-37 0.5909489345133636 1101 38-39 0.5771604993830977 1101 40-41 0.5574615034879784 1101 42-43 0.5506271666031637 1101 44-45 0.543012966185124 1101 46-47 0.5888236475729443 1101 48-49 0.5761184324641704 1101 50-51 0.5771446376014993 1101 52-53 0.6211452305383247 1101 54-55 0.5908895518439934 1101 56-57 0.5704922105719206 1101 58-59 0.576288426917209 1101 60-61 0.6496819813195813 1101 62-63 0.6520029592337337 1101 64-65 0.625544112916856 1101 66-67 0.6280316568243052 1101 68-69 0.645538136132636 1101 70-71 0.6918046679103824 1101 72-73 0.6956799515450882 1101 74-75 0.6765526962894342 1101 76-77 0.7261738948972152 1101 78-79 0.7293929792040217 1101 80-81 0.6945544422322172 1101 82-83 0.5996552735768361 1101 84-85 0.6624166508910321 1101 86-87 0.662102501202952 1101 88-89 0.6361613678630533 1101 90-91 0.6734524106318034 1101 92-93 0.7185759213934588 1101 94-95 0.7066430200820975 1101 96-97 0.7180776415062127 1101 98-99 0.7264813093348224 1101 100-101 0.5541128193075089 2304 1 0.09638177407910575 2304 2 0.1689339232764766 2304 3 0.18730050349057592 2304 4 0.24309653758622574 2304 5 0.23532443566614347 2304 6 0.2510799002976043 2304 7 0.2829722831859556 2304 8 0.26380145568764135 2304 9 0.27967389469667836 2304 10-11 0.26645398616238936 2304 12-13 0.26875217522243133 2304 14-15 0.4684097940818148 2304 16-17 0.50833350178101 2304 18-19 0.47223859996655904 2304 20-21 0.4957970715450415 2304 22-23 0.5031911905210862 2304 24-25 0.546043524278268 2304 26-27 0.49814706472997017 2304 28-29 0.5212745579988365 2304 30-31 0.5433173112324354 2304 32-33 0.5322616588488458 2304 34-35 0.5119027377494305 2304 36-37 0.5345084984334676 2304 38-39 0.5411992549163855 2304 40-41 0.4519251334513683 2304 42-43 0.5157259116486514 2304 44-45 0.571869239111571 2304 46-47 0.5837192634961923 2304 48-49 0.5525848099514334 2304 50-51 0.4976843638410813 2304 52-53 0.5394430519921372 2304 54-55 0.570790700208498 2304 56-57 0.5832077279412928 2304 58-59 0.6107296272095795 2304 60-61 0.5449001426024083 2304 62-63 0.6175441552072201 2304 64-65 0.6041903405551494 2304 66-67 0.6304608825432183 2304 68-69 0.6556493068495115 2304 70-71 0.632304284565194 2304 72-73 0.5845212170898009 2304 74-75 0.6277778199374282 2304 76-77 0.6877054199725166 2304 78-79 0.711543199790043 2304 80-81 0.6612498385411385 2304 82-83 0.6686804238835933 2304 84-85 0.6190208787484011 2304 86-87 0.5542400115703927 2304 88-89 0.6776519971882848 2304 90-91 0.6921514774255257 2304 92-93 0.6988602767099508 2304 94-95 0.6093642553245289 2304 96-97 0.6213139210017289 2304 98-99 0.6009858168225364 2304 100-101 0.6844413521381192 2202 1 0.13955834932004052 2202 2 0.20588339131082023 2202 3 0.22634252224912643 2202 4 0.30072374470010743 2202 5 0.3110346043958927 2202 6 0.3265442574441124 2202 7 0.32872151023929064 2202 8 0.34217879531628626 2202 9 0.35979633460501503 2202 10-11 0.34646447440589156 2202 12-13 0.34829707910938623 2202 14-15 0.5861883559768799 2202 16-17 0.5801462949227911 2202 18-19 0.5870397236778899 2202 20-21 0.5824412034179645 2202 22-23 0.6412403653815844 2202 24-25 0.644143131927585 2202 26-27 0.6406115624893189 2202 28-29 0.6546549568479705 2202 30-31 0.6784937194684559 2202 32-33 0.688007251338675 2202 34-35 0.6520712820583157 2202 36-37 0.6902476179066781 2202 38-39 0.7149793755312359 2202 40-41 0.69414478215581 2202 42-43 0.7251100558036754 2202 44-45 0.7141524462085798 2202 46-47 0.750279383548424 2202 48-49 0.6656312841889545 2202 50-51 0.6274263267892977 2202 52-53 0.6845339993430599 2202 54-55 0.7753048694951303 2202 56-57 0.8020532592858487 2202 58-59 0.8044924936209128 2202 60-61 0.8054701004659464 2202 62-63 0.8143212462111578 2202 64-65 0.8310128240908554 2202 66-67 0.8498617536036406 2202 68-69 0.8779372493032938 2202 70-71 0.8469129629380348 2202 72-73 0.8480432754347333 2202 74-75 0.8340733460057379 2202 76-77 0.8845064817907229 2202 78-79 0.8778201022666963 2202 80-81 0.8735113989140189 2202 82-83 0.9231644254344218 2202 84-85 0.8758206604202385 2202 86-87 0.9350513728439367 2202 88-89 0.911672059430984 2202 90-91 0.891667279477609 2202 92-93 0.9233284463817313 2202 94-95 0.9286928394745928 2202 96-97 0.9706661035637936 2202 98-99 0.9814336565263417 2202 100-101 1.0709659386444308 1102 1 0.08661342031547292 1102 2 0.07908543062013962 1102 3 0.13252254361694327 1102 4 0.17447655123526573 1102 5 0.1591337212506616 1102 6 0.13746821232672346 1102 7 0.16009014425639378 1102 8 0.1596444919882387 1102 9 0.17878712091150817 1102 10-11 0.15712005450814104 1102 12-13 0.16055887117573064 1102 14-15 0.28424846584950814 1102 16-17 0.31421765947943925 1102 18-19 0.26968044579410133 1102 20-21 0.2956839710879393 1102 22-23 0.3199497744263837 1102 24-25 0.2736496588480648 1102 26-27 0.31219371395103934 1102 28-29 0.3369200794376468 1102 30-31 0.34597003446400265 1102 32-33 0.3392217284506671 1102 34-35 0.3793573022847738 1102 36-37 0.3535750250238294 1102 38-39 0.4049810668883893 1102 40-41 0.36241697029532105 1102 42-43 0.3561735088547948 1102 44-45 0.3888592460740128 1102 46-47 0.4077711812513911 1102 48-49 0.3364102534605635 1102 50-51 0.25697374741883294 1102 52-53 0.29337375835561375 1102 54-55 0.3884367913812241 1102 56-57 0.4079718569724804 1102 58-59 0.4556817458636857 1102 60-61 0.4555406506586124 1102 62-63 0.37448314816492 1102 64-65 0.4061944648569451 1102 66-67 0.46177794209934575 1102 68-69 0.4179829866121523 1102 70-71 0.4241424575649475 1102 72-73 0.4631441273392767 1102 74-75 0.4416561569212476 1102 76-77 0.46490413962594346 1102 78-79 0.4710539508219682 1102 80-81 0.45879091762268587 1102 82-83 0.3971527195062876 1102 84-85 0.42448773519263483 1102 86-87 0.46824627538352104 1102 88-89 0.4893149042881788 1102 90-91 0.5225727670678921 1102 92-93 0.5319151438161285 1102 94-95 0.4178426770913717 1102 96-97 0.5002383864969033 1102 98-99 0.4917477205813867 1102 100-101 0.5258533719438994 2305 1 0.12931655588767654 2305 2 0.1444360017304831 2305 3 0.1160373410525466 2305 4 0.18598731361161924 2305 5 0.14648865101627706 2305 6 0.21074084148824568 2305 7 0.18669850502517704 2305 8 0.1869407080028722 2305 9 0.19666273621713515 2305 10-11 0.18297675055854512 2305 12-13 0.20262949863329993 2305 14-15 0.308029091923558 2305 16-17 0.3441269805469034 2305 18-19 0.335094991227777 2305 20-21 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-1.033582150786735 1213 84-85 -1.1689002921491891 1213 86-87 -1.0818488741290935 1213 88-89 -1.0543798691835278 1213 90-91 -1.1251106373619635 1213 92-93 -1.1404929088306162 1213 94-95 -0.9833548908292258 1213 96-97 -1.139481222623317 1213 98-99 -1.134734319804096 1213 100-101 -0.9704748783854704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25357.0 3 1812.0 4 1070.0 5 858.0 6 953.0 7 1226.0 8 2565.0 9 13009.0 10 53272.0 11 70092.0 12 41914.0 13 33151.0 14 32838.0 15 35021.0 16 39353.0 17 45407.0 18 51792.0 19 59317.0 20 67563.0 21 76317.0 22 86489.0 23 99554.0 24 114399.0 25 132005.0 26 155409.0 27 188720.0 28 222059.0 29 267817.0 30 336449.0 31 432510.0 32 571601.0 33 775786.0 34 1087729.0 35 1715612.0 36 3719269.0 37 9687607.0 38 5991354.0 39 5244.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.37269530830599 10.879603822375104 12.215208414554645 55.53249245476426 2 24.494629331515604 17.135377957251492 36.054836737491996 22.315155973740904 3 26.17344291979083 22.060212278079394 23.41401710082501 28.352327701304763 4 28.768334809704537 26.9032426575109 16.847029655336442 27.48139287744812 5 30.285940894087325 28.05177179394418 20.652819283190883 21.009468028777604 6 22.732047395911678 31.301098643363957 22.166995057861612 23.79985890286275 7 20.586337569872644 14.703078236190345 39.993276493045634 24.717307700891382 8 22.00530222139294 19.498167058695437 27.120874251181306 31.375656468730316 9 23.284294267962768 19.329947158815962 29.77639918126016 27.609359391961114 10-11 26.028060622363025 27.800815559731706 20.341799904152317 25.829323913752955 12-13 24.33204450111382 21.810887947624863 27.000313057424325 26.85675449383699 14-15 25.08131412571965 23.812993500059758 24.932677391372174 26.173014982848418 16-17 26.426306426341917 23.737863436656305 23.898953433104726 25.93687670389705 18-19 26.041314996434117 24.0714617953473 24.30285007580336 25.584373132415223 20-21 25.83407273210563 24.267695588119555 24.54781849562357 25.35041318415125 22-23 25.91964177377257 24.076247177577628 24.090745087831138 25.913365960818663 24-25 25.89461950217686 24.350680855254645 23.949908305939204 25.80479133662929 26-27 25.558195410646206 24.37656462484702 24.028338944673695 26.03690101983308 28-29 25.83641087235979 24.17829240587031 23.661874813538127 26.32342190823178 30-31 26.093804272341618 23.783925732530946 23.93784986528539 26.18442012984204 32-33 26.117328211468116 24.17582314887221 23.744611740755083 25.962236898904585 34-35 25.706915147901043 24.38369191196024 23.609782441570015 26.299610498568697 36-37 25.554291581933768 24.073397466413383 24.056648485883574 26.315662465769268 38-39 25.62184092091012 23.779895144248346 24.408827809658167 26.189436125183363 40-41 26.57408615933235 23.441194067313877 23.97053707405903 26.014182699294743 42-43 26.23742418260108 23.720365238034415 24.25728449438262 25.78492608498188 44-45 26.17242817845606 23.68782594318595 24.266038895475074 25.873706982882915 46-47 26.31024990564626 23.570333314009055 23.882615665434404 26.23680111491028 48-49 25.72169432302941 23.750058026748498 23.845575017591266 26.682672632630823 50-51 25.572356701848875 23.837982237645686 24.66720798225692 25.922453078248527 52-53 25.891226432970498 23.99334539841335 24.210607252423134 25.904820916193017 54-55 25.58788832650657 24.049082118489924 24.104979332775645 26.25805022222786 56-57 25.088100539885588 23.728155210916565 25.00089553034795 26.1828487188499 58-59 26.043714476546796 23.82775560978148 24.15847173940345 25.970058174268274 60-61 25.966024798927577 23.762398409896605 24.10357303066316 26.168003760512658 62-63 25.530295791562985 24.139395905557105 24.395427800260638 25.934880502619272 64-65 25.542659686518988 24.31559023842395 23.914568089056775 26.227181986000286 66-67 25.660163755310638 24.429625604848596 23.730245777959826 26.17996486188094 68-69 25.5652012673418 24.4173044406467 24.009518894092906 26.0079753979186 70-71 25.917265028503213 24.184156041477298 23.663838608912 26.2347403211075 72-73 25.83013168372671 24.42876915016626 23.883734742507443 25.857364423599584 74-75 26.08584158494635 24.04601265566355 24.030426524675047 25.837719234715053 76-77 26.26578235913628 24.032258116849746 23.869705159546186 25.832254364467786 78-79 25.952581742660886 24.05225265618057 23.790830582548306 26.20433501861024 80-81 25.925063693523125 24.186070473347463 23.620986210906775 26.267879622222633 82-83 26.061008554670728 24.01751889061703 23.58815997736377 26.333312577348472 84-85 25.878840974104346 24.161673573625023 23.524416520686344 26.435068931584293 86-87 25.79811851013045 24.688629336499545 23.646646633661554 25.866605519708447 88-89 26.0271014053008 24.28952361402689 23.611003138883806 26.072371841788506 90-91 26.233999883956926 24.028874309878088 23.593750945044142 26.143374861120844 92-93 25.910221942487667 24.410360294353563 24.116920049789123 25.562497713369652 94-95 25.995289512365737 24.346664620303844 23.66187699883038 25.996168868500035 96-97 25.565387048632747 24.693474528881083 23.63380212104255 26.107336301443617 98-99 25.89586583474498 24.412436630890753 23.9096040067904 25.782093527573867 100-101 26.13838537237969 24.47686290935711 23.50822099819295 25.87653072007025 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 22721.0 1 12076.5 2 1131.5 3 709.0 4 569.5 5 557.0 6 549.5 7 513.0 8 501.5 9 512.0 10 515.0 11 580.0 12 713.0 13 852.5 14 943.5 15 1076.0 16 1324.5 17 1715.0 18 2035.5 19 2167.0 20 2534.5 21 3373.5 22 4833.0 23 7150.0 24 9970.5 25 13959.0 26 19574.5 27 26719.0 28 37036.0 29 50784.5 30 65572.5 31 82122.5 32 103829.5 33 129159.5 34 159942.5 35 211215.0 36 267674.5 37 341228.0 38 414600.0 39 467306.5 40 533413.5 41 601559.5 42 664873.5 43 732512.0 44 814576.0 45 849521.0 46 852481.0 47 903851.0 48 899498.0 49 889454.5 50 987610.0 51 1000774.0 52 957270.5 53 984713.5 54 1028729.5 55 1070626.0 56 1119040.5 57 1108224.5 58 958489.5 59 779339.5 60 704121.0 61 642640.5 62 558421.0 63 500616.0 64 432215.0 65 397415.0 66 391495.5 67 384520.0 68 358262.5 69 300069.0 70 256545.5 71 220057.5 72 177517.0 73 146408.0 74 143693.0 75 130856.0 76 87479.5 77 55977.0 78 42370.5 79 41504.5 80 34193.0 81 20879.0 82 9426.5 83 2637.5 84 1680.5 85 1201.0 86 963.5 87 796.0 88 649.5 89 544.5 90 456.5 91 376.0 92 291.0 93 213.0 94 159.5 95 118.0 96 79.5 97 51.5 98 26.5 99 16.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1266876250357245 2 0.112649328379537 3 0.10927693626750501 4 0.12132228255692104 5 0.1234562255882633 6 0.11185291035533962 7 0.11427645994093552 8 0.11322473087548825 9 0.11067924168810135 10-11 0.0987101076498047 12-13 0.10844812803658188 14-15 0.14227303038963512 16-17 0.15267600266742878 18-19 0.14174335524435555 20-21 0.16494617509764695 22-23 0.14756025531104125 24-25 0.1342897970848814 26-27 0.14075259597980375 28-29 0.12978565304372677 30-31 0.10036391349909499 32-33 0.09911022196818138 34-35 0.12220634466990568 36-37 0.12174716585691149 38-39 0.1303591502334 40-41 0.16617509764694674 42-43 0.13416214156425646 44-45 0.13446508526245596 46-47 0.1546003620081928 48-49 0.18170524911879585 50-51 0.1535162427360198 52-53 0.11302657902257787 54-55 0.11470896446603791 56-57 0.11044298370963132 58-59 0.11255025245308183 60-61 0.11476231304182148 62-63 0.09997142040583025 64-65 0.1109269315042393 66-67 0.13144708011812897 68-69 0.12393064685148136 70-71 0.11337143945889303 72-73 0.11966276078879681 74-75 0.16357244927122036 76-77 0.17455082404496525 78-79 0.1708183290463942 80-81 0.14446984852815092 82-83 0.1264818519577022 84-85 0.13196151281318472 86-87 0.12354005906449461 88-89 0.12705534914737546 90-91 0.13990092407354482 92-93 0.11564828046108412 94-95 0.11484995713060873 96-97 0.11850052395922644 98-99 0.11621415642564542 100-101 0.10871677622177765 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.62425E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.91140738617953 #Duplication Level Percentage of deduplicated Percentage of total 1 73.41492906809344 21.959438515832165 2 13.092012893529976 7.832010623269802 3 4.713930953219821 4.23000927396239 4 2.469624329712903 2.954797576674528 5 1.4895130580707052 2.227671594349348 6 0.8484439944261426 1.522689237698267 7 0.5829593264966714 1.2205993733090348 8 0.4446493745846579 1.0640070869769318 9 0.32457153331892036 0.8737552223153253 >10 2.1579394568747605 12.004020218703529 >50 0.21352662475163983 4.496113712308536 >100 0.19072951360732626 11.975956586043406 >500 0.029502488419506707 6.190863111358957 >1k 0.024976850070698626 14.618521506459869 >5k 0.0021191817996523096 4.340825342502356 >10k+ 5.713530231886126E-4 2.488721018235996 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGACATGCGTGCGAGTCGACGGGTGCGGAAACCCGGAAGGCACAAGGAA 27683 0.10548918738687244 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.035415833095170046 1.1431837667905116E-5 7.621225111936744E-6 2 0.03554920453462894 1.1431837667905116E-5 7.621225111936744E-6 3 0.03601790987901305 1.1431837667905116E-5 1.1431837667905116E-5 4 0.03761074592740783 1.5242450223873488E-5 1.905306277984186E-5 5 0.037934647994665144 1.905306277984186E-5 1.905306277984186E-5 6 0.03893683909688483 3.0484900447746976E-5 2.6674287891778602E-5 7 0.039074021148899685 3.0484900447746976E-5 3.0484900447746976E-5 8 0.03944746117938459 3.429551300371535E-5 3.810612555968372E-5 9 0.040243879203581974 4.572735067162046E-5 3.810612555968372E-5 10-11 0.04143469562732209 5.1443269505573025E-5 4.382204439363628E-5 12-13 0.04255882633133276 6.478041345146232E-5 4.763265694960465E-5 14-15 0.04390587786986758 6.668571972944651E-5 5.3348575783557204E-5 16-17 0.045070020005715916 8.002286367533581E-5 5.3348575783557204E-5 18-19 0.04619034009717062 8.383347623130418E-5 6.096980089549395E-5 20-21 0.0471810993617224 8.383347623130418E-5 6.478041345146232E-5 22-23 0.048114699437934644 9.336000762122512E-5 6.85910260074307E-5 24-25 0.048937791750023815 1.0288653901114604E-4 6.85910260074307E-5 26-27 0.04966561874821378 1.1050776412308279E-4 6.85910260074307E-5 28-29 0.05036486615223397 1.1050776412308279E-4 7.240163856339907E-5 30-31 0.050982185386300845 1.1431837667905116E-4 8.383347623130418E-5 32-33 0.05154996665714014 1.1812898923501953E-4 8.383347623130418E-5 34-35 0.05211203200914547 1.2003429551300373E-4 9.907592645517767E-5 36-37 0.052740783080880246 1.2575021434695628E-4 1.0669715156711441E-4 38-39 0.053420977422120604 1.3337143945889302E-4 1.0669715156711441E-4 40-41 0.05398304277412594 1.3337143945889302E-4 1.086024578450986E-4 42-43 0.05457559302657902 1.352767457368772E-4 1.219396017909879E-4 44-45 0.05525769267409736 1.5242450223873488E-4 1.2575021434695628E-4 46-47 0.055884538439554154 1.5814042107268743E-4 1.2575021434695628E-4 48-49 0.056602838906354196 1.6766695246260837E-4 1.2575021434695628E-4 50-51 0.05750976469467467 1.7147756501857672E-4 1.2575021434695628E-4 52-53 0.05855958845384396 1.7147756501857672E-4 1.2765552062494047E-4 54-55 0.05953129465561589 1.8481470896446604E-4 1.3146613318090882E-4 56-57 0.060634466990568736 1.9624654663237117E-4 1.4099266457082976E-4 58-59 0.06195674954748975 2.0005715918833952E-4 1.4289797084881395E-4 60-61 0.06350766885776889 2.1529960941221303E-4 1.4480327712679814E-4 62-63 0.06554634657521197 2.2673144708011813E-4 1.505191959607507E-4 64-65 0.06773173287605982 2.3435267219205487E-4 1.5242450223873488E-4 66-67 0.06994950938363342 2.3625797847003907E-4 1.5623511479470324E-4 68-69 0.07274649899971421 2.4006859102600742E-4 1.6385633990663998E-4 70-71 0.07655330094312661 2.438792035819758E-4 1.6766695246260837E-4 72-73 0.0816747642183481 2.438792035819758E-4 1.6766695246260837E-4 74-75 0.08806516147470705 2.572163475278651E-4 1.7147756501857672E-4 76-77 0.09588453843955416 2.6483757263980187E-4 1.771934838525293E-4 78-79 0.10602648375726398 2.6674287891778603E-4 1.886253215204344E-4 80-81 0.12036391349909498 2.7245879775173864E-4 2.0005715918833952E-4 82-83 0.1407716490425836 2.7626941030770697E-4 2.0767838430027627E-4 84-85 0.16974373630561113 2.819853291416595E-4 2.2482614080213394E-4 86-87 0.20773173287605984 2.857959416976279E-4 2.3054205963608652E-4 88-89 0.2547966085548252 3.105649233114223E-4 2.3435267219205487E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAT 3875 0.0 37.626755 1 ACATGCG 8730 0.0 34.327675 4 CATGCGT 8910 0.0 33.099766 5 GCGTATT 4775 0.0 29.937859 2 TAAGTCG 4855 0.0 29.155365 9 TCCGTAC 9430 0.0 23.418137 5 GTCCGTA 9555 0.0 22.665403 4 TTTAAAA 18380 0.0 22.024982 56-57 CTGACAT 15665 0.0 21.828918 1 TGTCCGT 10050 0.0 21.407276 3 GTTAGTT 8675 0.0 19.982668 1 TAAAAAA 31495 0.0 18.733091 58-59 ATGCGTG 18060 0.0 18.4566 6 TTGTCCG 12245 0.0 18.306732 2 TTAAAAA 32970 0.0 18.248383 56-57 TACCCTA 9630 0.0 17.954218 9 CCGTACC 13925 0.0 17.86769 6 TGCGTGC 18815 0.0 17.741014 7 CGTCAAT 14760 0.0 17.599972 5 CGTGCGA 16985 0.0 17.478569 9 >>END_MODULE