FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n02 mx0-1.3420000000ca34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n02 mx0-1.3420000000ca34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26242500
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTGCGGAAACCCGGAAGGCACAAGGAA276830.10548918738687244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT38750.037.6267551
ACATGCG87300.034.3276754
CATGCGT89100.033.0997665
GCGTATT47750.029.9378592
TAAGTCG48550.029.1553659
TCCGTAC94300.023.4181375
GTCCGTA95550.022.6654034
TTTAAAA183800.022.02498256-57
CTGACAT156650.021.8289181
TGTCCGT100500.021.4072763
GTTAGTT86750.019.9826681
TAAAAAA314950.018.73309158-59
ATGCGTG180600.018.45666
TTGTCCG122450.018.3067322
TTAAAAA329700.018.24838356-57
TACCCTA96300.017.9542189
CCGTACC139250.017.867696
TGCGTGC188150.017.7410147
CGTCAAT147600.017.5999725
CGTGCGA169850.017.4785699
Produced by FastQC (version 0.11.2)