##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l05n02 m48-2.3420000000cc9f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22381281 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38570714518083 33.0 33.0 33.0 33.0 33.0 2 32.350924462277206 33.0 33.0 33.0 33.0 33.0 3 32.340378551165145 33.0 33.0 33.0 33.0 33.0 4 36.16967929583655 37.0 37.0 37.0 37.0 37.0 5 36.16816593295084 37.0 37.0 37.0 37.0 37.0 6 36.16979948556117 37.0 37.0 37.0 37.0 37.0 7 36.2145564858419 37.0 37.0 37.0 37.0 37.0 8 36.196021755859284 37.0 37.0 37.0 37.0 37.0 9 36.1763110431436 37.0 37.0 37.0 37.0 37.0 10-11 36.16402061615687 37.0 37.0 37.0 37.0 37.0 12-13 36.160094209978425 37.0 37.0 37.0 37.0 37.0 14-15 38.3622837316595 40.0 37.0 40.0 37.0 40.0 16-17 38.31540574018082 40.0 37.0 40.0 37.0 40.0 18-19 38.31660285664614 40.0 37.0 40.0 37.0 40.0 20-21 38.28273149334035 40.0 37.0 40.0 37.0 40.0 22-23 38.28432195190257 40.0 37.0 40.0 37.0 40.0 24-25 38.223085488270314 40.0 37.0 40.0 37.0 40.0 26-27 38.17453938405045 40.0 37.0 40.0 37.0 40.0 28-29 38.11274729985294 40.0 37.0 40.0 37.0 40.0 30-31 38.058221421731844 40.0 37.0 40.0 35.0 40.0 32-33 37.97425942688446 40.0 37.0 40.0 33.0 40.0 34-35 37.90708487597291 40.0 37.0 40.0 33.0 40.0 36-37 37.83344447531846 40.0 37.0 40.0 33.0 40.0 38-39 37.73765844770011 40.0 37.0 40.0 33.0 40.0 40-41 37.628539626485185 40.0 37.0 40.0 33.0 40.0 42-43 37.51424076217978 40.0 37.0 40.0 33.0 40.0 44-45 37.4025088420989 40.0 37.0 40.0 33.0 40.0 46-47 37.234948236430256 40.0 37.0 40.0 33.0 40.0 48-49 37.112902496510365 40.0 37.0 40.0 33.0 40.0 50-51 35.62768241460353 38.5 35.0 38.5 30.0 38.5 52-53 35.963578067761176 37.0 35.0 38.5 33.0 40.0 54-55 36.687620806869816 37.0 37.0 40.0 33.0 40.0 56-57 36.656356421243274 37.0 37.0 40.0 33.0 40.0 58-59 36.529151704944866 37.0 37.0 40.0 33.0 40.0 60-61 36.36437554669011 37.0 37.0 40.0 33.0 40.0 62-63 36.18260849323146 37.0 37.0 40.0 33.0 40.0 64-65 35.98839753631617 37.0 37.0 40.0 33.0 40.0 66-67 35.73664702212532 37.0 37.0 38.5 33.0 40.0 68-69 35.50161954536919 37.0 37.0 37.0 33.0 40.0 70-71 35.296476171314765 37.0 37.0 37.0 33.0 40.0 72-73 35.090123170340426 37.0 35.0 37.0 33.0 40.0 74-75 34.88764195400612 37.0 33.0 37.0 30.0 37.0 76-77 34.70219718433454 37.0 33.0 37.0 27.0 37.0 78-79 34.52912199261517 37.0 33.0 37.0 27.0 37.0 80-81 34.40157361859672 37.0 33.0 37.0 27.0 37.0 82-83 34.29616046105672 37.0 33.0 37.0 27.0 37.0 84-85 34.206082306012775 37.0 33.0 37.0 27.0 37.0 86-87 34.12011077471392 37.0 33.0 37.0 27.0 37.0 88-89 34.04228178449661 37.0 33.0 37.0 27.0 37.0 90-91 33.94396795250459 37.0 33.0 37.0 27.0 37.0 92-93 33.882194812709784 37.0 33.0 37.0 27.0 37.0 94-95 33.81282023133528 37.0 33.0 37.0 27.0 37.0 96-97 33.73099698806337 37.0 33.0 37.0 27.0 37.0 98-99 33.64985123058863 37.0 33.0 37.0 27.0 37.0 100-101 31.905008162848233 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10883189269382143 2306 2 0.25036678500359955 2306 3 0.2730926971198855 2306 4 0.3664447808929694 2306 5 0.39485877212538867 2306 6 0.3647324506529728 2306 7 0.38184588365220407 2306 8 0.37774763686271484 2306 9 0.40188072496842864 2306 10-11 0.4095506983588635 2306 12-13 0.3963631078775265 2306 14-15 0.6828323497874109 2306 16-17 0.6574986654867558 2306 18-19 0.6573214967269649 2306 20-21 0.7059391985888581 2306 22-23 0.6497759806733825 2306 24-25 0.6649912020227262 2306 26-27 0.7068227364075099 2306 28-29 0.6525137526969615 2306 30-31 0.7205506933653467 2306 32-33 0.7409783193757917 2306 34-35 0.7212845016857088 2306 36-37 0.6759045546268041 2306 38-39 0.6965635926199099 2306 40-41 0.7430281867576554 2306 42-43 0.7626759543494259 2306 44-45 0.7773647243330828 2306 46-47 0.8161481073550121 2306 48-49 0.8037372774859364 2306 50-51 0.6479552979677976 2306 52-53 0.7193456844613522 2306 54-55 0.7924627924055869 2306 56-57 0.7702069509370162 2306 58-59 0.7851218315240232 2306 60-61 0.7460510954030113 2306 62-63 0.7769808026934584 2306 64-65 0.7842141491936019 2306 66-67 0.7372104000922164 2306 68-69 0.7654318943104599 2306 70-71 0.7808507667214997 2306 72-73 0.7498969472123207 2306 74-75 0.7412976664586282 2306 76-77 0.8136497704305512 2306 78-79 0.7925179063778174 2306 80-81 0.6920475384721598 2306 82-83 0.7648089798332904 2306 84-85 0.8255062843785126 2306 86-87 0.8231376608859406 2306 88-89 0.7957342886531364 2306 90-91 0.8156094716779521 2306 92-93 0.82642841342971 2306 94-95 0.7879638126543611 2306 96-97 0.8015618148764148 2306 98-99 0.7663278104860041 2306 100-101 0.4648264390888244 2307 1 0.014178526969025995 2307 2 0.1306479342847453 2307 3 0.17923861847872757 2307 4 0.23632145481137456 2307 5 0.27650966786448805 2307 6 0.2834526436269016 2307 7 0.2200180326451857 2307 8 0.25817968219190846 2307 9 0.23381743835778934 2307 10-11 0.25074193168803305 2307 12-13 0.2845563965094442 2307 14-15 0.46780243047873427 2307 16-17 0.4974474043725152 2307 18-19 0.4437517750966933 2307 20-21 0.5147727972876055 2307 22-23 0.4928085884506004 2307 24-25 0.48109677714100485 2307 26-27 0.49608920300452297 2307 28-29 0.46303662780216115 2307 30-31 0.48951733556348387 2307 32-33 0.4262800059474756 2307 34-35 0.5161564385910253 2307 36-37 0.4937404911595351 2307 38-39 0.4634982478487757 2307 40-41 0.5015409125576937 2307 42-43 0.46737802659014704 2307 44-45 0.4519685115414944 2307 46-47 0.4770121864279204 2307 48-49 0.4452873478831805 2307 50-51 0.37284201080105106 2307 52-53 0.35127746533266446 2307 54-55 0.4752967331463154 2307 56-57 0.47956411484938855 2307 58-59 0.5396177338749553 2307 60-61 0.5438569464719691 2307 62-63 0.47789607394699374 2307 64-65 0.5274461387348452 2307 66-67 0.5974615363188462 2307 68-69 0.610228111415708 2307 70-71 0.5487600649264266 2307 72-73 0.5468744509523091 2307 74-75 0.5078265885278128 2307 76-77 0.5290923910823153 2307 78-79 0.547045466052495 2307 80-81 0.5398690188361996 2307 82-83 0.5608306911384275 2307 84-85 0.5556556290342556 2307 86-87 0.5571726451540187 2307 88-89 0.5921678058403117 2307 90-91 0.5321682278059967 2307 92-93 0.49019390698157395 2307 94-95 0.5804197208281536 2307 96-97 0.41484726551798445 2307 98-99 0.3734647769640347 2307 100-101 0.3768377703060324 2201 1 0.16605099134184087 2201 2 0.24196773209822453 2201 3 0.2424676943142785 2201 4 0.27594693386667757 2201 5 0.3199447531017938 2201 6 0.3312486834787407 2201 7 0.3219539422088218 2201 8 0.34493706251571865 2201 9 0.35740375613137587 2201 10-11 0.3588575610662659 2201 12-13 0.3559441618131629 2201 14-15 0.579653347012119 2201 16-17 0.6289082223723739 2201 18-19 0.6114285092680305 2201 20-21 0.6256492978953574 2201 22-23 0.5701147560576487 2201 24-25 0.6161352467612033 2201 26-27 0.5821255964538636 2201 28-29 0.6166888098107961 2201 30-31 0.600817734907956 2201 32-33 0.6359210755406295 2201 34-35 0.6560604673806978 2201 36-37 0.6351257584717729 2201 38-39 0.6144753918407488 2201 40-41 0.6708037284628503 2201 42-43 0.6140370981129166 2201 44-45 0.5688521027157378 2201 46-47 0.6762879635513102 2201 48-49 0.6633322966588509 2201 50-51 0.6371856686144639 2201 52-53 0.6988677168350677 2201 54-55 0.65673125920312 2201 56-57 0.6140897301350705 2201 58-59 0.6412712399084697 2201 60-61 0.708152313208636 2201 62-63 0.7310150211150557 2201 64-65 0.7148779099250078 2201 66-67 0.7333401906412007 2201 68-69 0.7627195786791461 2201 70-71 0.7463499129206213 2201 72-73 0.7755338250978809 2201 74-75 0.7679849410206785 2201 76-77 0.780688159519336 2201 78-79 0.7767688424641292 2201 80-81 0.7937248008097058 2201 82-83 0.7392090247427703 2201 84-85 0.7429255729661648 2201 86-87 0.8064447201104983 2201 88-89 0.8080358612635905 2201 90-91 0.8451013621187258 2201 92-93 0.7896812184780799 2201 94-95 0.7979751631897756 2201 96-97 0.7959381905532723 2201 98-99 0.7431757284139948 2201 100-101 0.5912232004083506 1101 1 0.20937183413991534 1101 2 0.220890597579384 1101 3 0.2494484421824552 1101 4 0.2925228483214397 1101 5 0.3445578802223892 1101 6 0.301611317779944 1101 7 0.3518801910004612 1101 8 0.34686265742222133 1101 9 0.36803337249924084 1101 10-11 0.37300999038944127 1101 12-13 0.3618518451547743 1101 14-15 0.5721629277981677 1101 16-17 0.628189237084527 1101 18-19 0.5622854230756431 1101 20-21 0.5821250100694684 1101 22-23 0.529715234030455 1101 24-25 0.5467630013393006 1101 26-27 0.5783416902228922 1101 28-29 0.6131912587856121 1101 30-31 0.6143613424918257 1101 32-33 0.6222000001212322 1101 34-35 0.6160304648435471 1101 36-37 0.6675993475670978 1101 38-39 0.6222087178487996 1101 40-41 0.6467684239960008 1101 42-43 0.6489412308479814 1101 44-45 0.6027776081165825 1101 46-47 0.6617478753132531 1101 48-49 0.6283914427409414 1101 50-51 0.659789680795484 1101 52-53 0.6884894661774794 1101 54-55 0.6208590643088527 1101 56-57 0.6081798200694308 1101 58-59 0.6260411552050087 1101 60-61 0.6716104352943049 1101 62-63 0.6496184658748447 1101 64-65 0.6403002104921143 1101 66-67 0.6700746126115789 1101 68-69 0.6749939904524425 1101 70-71 0.6878690340540601 1101 72-73 0.6802343239759239 1101 74-75 0.6587236222943957 1101 76-77 0.685753007294899 1101 78-79 0.7226720444247903 1101 80-81 0.6924054519458238 1101 82-83 0.6181625855112998 1101 84-85 0.678758888844591 1101 86-87 0.6886926880394171 1101 88-89 0.6957395214347386 1101 90-91 0.7304275680428134 1101 92-93 0.7520983800145586 1101 94-95 0.7055181082451227 1101 96-97 0.7043122589450235 1101 98-99 0.7170246427145557 1101 100-101 0.6893995819338272 2304 1 0.1137764033144677 2304 2 0.20113000523828362 2304 3 0.21335476911456652 2304 4 0.26639266059604694 2304 5 0.2857952524060181 2304 6 0.305227303219489 2304 7 0.32054716563501984 2304 8 0.3223722094957253 2304 9 0.3234313343329873 2304 10-11 0.31174456051845567 2304 12-13 0.3010989386494174 2304 14-15 0.5194926717078303 2304 16-17 0.5679409912330939 2304 18-19 0.5145002165224852 2304 20-21 0.5341494305869148 2304 22-23 0.5267696709054235 2304 24-25 0.5849955842765482 2304 26-27 0.5724980976391905 2304 28-29 0.6074083429304977 2304 30-31 0.5935671013885653 2304 32-33 0.5741281059238972 2304 34-35 0.5576352155752673 2304 36-37 0.5808138855108425 2304 38-39 0.5990075899631222 2304 40-41 0.5299672175953347 2304 42-43 0.5728012567795204 2304 44-45 0.6183236888631285 2304 46-47 0.6357899980043555 2304 48-49 0.593244981164446 2304 50-51 0.5593717237073861 2304 52-53 0.6097863987528385 2304 54-55 0.6473006805908668 2304 56-57 0.6262834615421085 2304 58-59 0.6454122840283247 2304 60-61 0.5859569409356311 2304 62-63 0.6328065235790206 2304 64-65 0.6078491208337908 2304 66-67 0.6535755459757198 2304 68-69 0.6981763725681773 2304 70-71 0.6884046129647885 2304 72-73 0.6635913879356394 2304 74-75 0.6742020605944674 2304 76-77 0.7275210529541383 2304 78-79 0.7658910301966415 2304 80-81 0.7253380941547434 2304 82-83 0.7483731001573162 2304 84-85 0.7003340086458323 2304 86-87 0.6903747196968482 2304 88-89 0.7856176185688071 2304 90-91 0.7965628511137552 2304 92-93 0.7941649354166174 2304 94-95 0.7605524406847834 2304 96-97 0.7462387922053395 2304 98-99 0.6895111398035922 2304 100-101 0.6804655687258716 2202 1 0.15526342546300498 2202 2 0.19523811892130283 2202 3 0.24486814269539536 2202 4 0.3199535493237349 2202 5 0.34202094905333524 2202 6 0.3329667382512653 2202 7 0.3371278057017477 2202 8 0.3785269645984002 2202 9 0.3939335383173699 2202 10-11 0.3562225445445577 2202 12-13 0.37815822884665096 2202 14-15 0.6004672956373227 2202 16-17 0.5991796924541148 2202 18-19 0.6083041545400931 2202 20-21 0.6172312645973577 2202 22-23 0.6048202398939964 2202 24-25 0.666449377018246 2202 26-27 0.653920270313499 2202 28-29 0.6819263742879969 2202 30-31 0.7096797194205564 2202 32-33 0.6831760315123674 2202 34-35 0.6755843759114128 2202 36-37 0.6983764253127234 2202 38-39 0.7566217434730902 2202 40-41 0.7200110210529473 2202 42-43 0.7579973830153861 2202 44-45 0.7602553585918486 2202 46-47 0.7573278689743645 2202 48-49 0.7370407561379508 2202 50-51 0.6744648131857645 2202 52-53 0.7046447093247252 2202 54-55 0.8330594676293117 2202 56-57 0.8482122428668006 2202 58-59 0.8313393461221068 2202 60-61 0.8574350997071676 2202 62-63 0.8285287919729996 2202 64-65 0.8085383369683825 2202 66-67 0.8139724042808965 2202 68-69 0.8641871908722507 2202 70-71 0.866034768293062 2202 72-73 0.856417093019985 2202 74-75 0.8433301596613205 2202 76-77 0.8794224492362659 2202 78-79 0.8398737872953816 2202 80-81 0.8665336637272958 2202 82-83 0.9012190300747918 2202 84-85 0.8861647318067227 2202 86-87 0.953631623417813 2202 88-89 0.9244865779537008 2202 90-91 0.8734224751288195 2202 92-93 0.9052826599487389 2202 94-95 0.9060223790644955 2202 96-97 0.938988128728127 2202 98-99 0.9552986209741761 2202 100-101 0.9997541701288171 1102 1 0.07658432812672089 1102 2 0.08708472382632237 1102 3 0.10048423282771068 1102 4 0.1345632752207777 1102 5 0.11046948473639873 1102 6 0.10397360316218851 1102 7 0.12620173668211265 1102 8 0.1665005657291232 1102 9 0.15717658930937972 1102 10-11 0.12134501834287192 1102 12-13 0.12546946178770213 1102 14-15 0.2275721124455501 1102 16-17 0.26671644585110243 1102 18-19 0.21442706311405146 1102 20-21 0.2451128226259769 1102 22-23 0.2883264648242232 1102 24-25 0.26577683442071987 1102 26-27 0.2868193925992628 1102 28-29 0.3107657700804367 1102 30-31 0.29910145805840216 1102 32-33 0.3099521835392878 1102 34-35 0.34295956631208924 1102 36-37 0.2812730853434644 1102 38-39 0.32336311910361104 1102 40-41 0.2686364581931713 1102 42-43 0.25978981728190575 1102 44-45 0.3299991689248074 1102 46-47 0.33987094610021273 1102 48-49 0.3123364117948313 1102 50-51 0.20779477612548192 1102 52-53 0.26387837897090805 1102 54-55 0.356513217741373 1102 56-57 0.35647021697477754 1102 58-59 0.3565931640052966 1102 60-61 0.4027878966438223 1102 62-63 0.33165798911542765 1102 64-65 0.3847718213542919 1102 66-67 0.4076907265219134 1102 68-69 0.37072257599566427 1102 70-71 0.3701304647853334 1102 72-73 0.36452641940458363 1102 74-75 0.3641733540090115 1102 76-77 0.39993475557655955 1102 78-79 0.38661376165091355 1102 80-81 0.39388158066982726 1102 82-83 0.34045596079526064 1102 84-85 0.3955718053770454 1102 86-87 0.40968082112362936 1102 88-89 0.42064233732753564 1102 90-91 0.4281757145105871 1102 92-93 0.45574468745280683 1102 94-95 0.3877280812975741 1102 96-97 0.4364113454386427 1102 98-99 0.4495272802655208 1102 100-101 0.45663489294408066 2305 1 0.14060840300764 2305 2 0.13045587537574477 2305 3 0.11470580948925857 2305 4 0.18077815113252882 2305 5 0.15324222124412756 2305 6 0.19661904937156294 2305 7 0.1871233060820785 2305 8 0.20678765546849576 2305 9 0.20574496453592417 2305 10-11 0.21258724399761775 2305 12-13 0.19651072578363937 2305 14-15 0.301697332031452 2305 16-17 0.37873531227798196 2305 18-19 0.3367963872915922 2305 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-1.219739361622807 1213 86-87 -1.150580204413444 1213 88-89 -1.1171041643552826 1213 90-91 -1.1928942021569071 1213 92-93 -1.1799652535755598 1213 94-95 -1.0650350501171104 1213 96-97 -1.2093682054381105 1213 98-99 -1.2285620225122145 1213 100-101 -1.0643250961116166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22075.0 3 1588.0 4 896.0 5 778.0 6 776.0 7 1191.0 8 2264.0 9 8231.0 10 28715.0 11 40087.0 12 29794.0 13 26395.0 14 27395.0 15 29644.0 16 33695.0 17 38801.0 18 44374.0 19 50070.0 20 56731.0 21 64841.0 22 73323.0 23 83653.0 24 96798.0 25 113016.0 26 136438.0 27 174569.0 28 193029.0 29 216132.0 30 267767.0 31 341971.0 32 451350.0 33 611428.0 34 859984.0 35 1332354.0 36 2801288.0 37 7467194.0 38 6647122.0 39 5524.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.115158257332837 12.680580125522845 14.96910872481564 50.235152892328685 2 23.741730208190752 17.557522860187174 36.32695994625026 22.373786985371808 3 25.440921554956695 22.50440267376969 25.41275499913872 26.641920772134892 4 27.687982156512735 26.917108827539053 18.737357112839444 26.657551903108768 5 29.154360167864514 29.018020691336343 21.534801528222552 20.292817612576588 6 22.23568489683385 32.65583977768113 22.234763429017924 22.873711896467096 7 20.093732817230713 15.879750630321906 41.00875839243803 23.017758160009347 8 21.718940452557717 20.827664138918312 27.4719532835567 29.981442124967273 9 22.539810725303703 20.593928074613437 30.221753264776265 26.644507935306592 10-11 25.266995346737826 28.795714316958083 21.07679181856674 24.86049851773735 12-13 23.548413992307925 22.886855069341767 27.657467318694433 25.907263619655872 14-15 24.09452816358654 24.69818835942273 26.316769626188286 24.890513850802446 16-17 25.08557415964007 24.632993572523763 25.250538039515703 25.030894228320467 18-19 24.975488141428496 25.01225956481526 25.492661789956202 24.519590503800046 20-21 24.640130783048374 25.170876353076725 25.660739912695686 24.528252951179216 22-23 24.951160102504495 24.908596072546434 25.254419866411954 24.885823958537124 24-25 24.846327164764993 25.21741171040538 25.084043729181456 24.852217395648175 26-27 24.374701862116478 25.51496726562934 25.166888979481488 24.943441892772693 28-29 24.773967498296262 25.208401770835398 24.849463220693732 25.168167510174612 30-31 24.96897929387154 24.75158500456181 25.224086389161183 25.05534931240547 32-33 24.877183767433106 25.032334302743713 25.1006547455906 24.989827184232578 34-35 24.683890654809986 25.320002506117493 24.730241749747794 25.265865089324723 36-37 24.369332908671137 25.163348920559415 25.21187162422129 25.255446546548164 38-39 24.449530203122862 24.903951169498683 25.386345623048523 25.26017300432993 40-41 25.255123973559385 24.64289491375802 24.91626704371542 25.18571406896718 42-43 24.971256428140936 24.82724719028859 25.41744256693749 24.78405381463299 44-45 25.20670853226165 24.6868609271138 25.22723828121461 24.879192259409944 46-47 24.985387104730496 24.6641059072927 25.136650121383862 25.21385686659294 48-49 24.650378229832814 25.02748898650018 24.8663532119455 25.4557795717215 50-51 24.475154534521845 24.914474910869156 25.530444846366752 25.07992570824225 52-53 24.70502639733667 25.17140432041815 25.293072502373583 24.8304967798716 54-55 24.530183782450994 25.082929926515828 25.186935658025416 25.199950633007766 56-57 24.134462777291592 25.042502654717545 25.83138636899745 24.99164819899341 58-59 24.72017081424166 25.043625704317318 25.306321532411268 24.929881949029753 60-61 24.588431469495593 25.10439989502194 25.23956116235782 25.06760747312465 62-63 24.33581825503028 25.2860204850506 25.396712410891077 24.981448849028048 64-65 24.535960667726478 25.41825162366044 24.91902749300011 25.12676021561297 66-67 24.508076333331424 25.42611968772012 24.833002494001224 25.232801484947228 68-69 24.505101021801458 25.370066860983425 25.06629808413149 25.058534033083625 70-71 24.739230469854057 25.31544195288118 24.797462431986013 25.147865145278747 72-73 24.660040868863504 25.393365830815394 25.101909341517782 24.84468395880332 74-75 25.00273621367328 25.15621480782225 25.001445029629544 24.83960394887493 76-77 25.227191849544923 25.099729649168317 24.826673093562928 24.846405407723836 78-79 24.7767517160404 25.16213320602909 24.88720822859729 25.173906849333218 80-81 24.937932483643905 25.28026810518088 24.713113925192513 25.0686854859827 82-83 24.93039715657708 25.25125819513742 24.661318132668388 25.157026515617115 84-85 24.869873568457134 25.11507351278214 24.608282966417786 25.406769952342938 86-87 24.74791203550647 25.525405637067948 24.77458170250135 24.952100624924228 88-89 24.950198425613937 25.38624489172321 24.72492311161248 24.938633571050374 90-91 25.151517998865735 25.115655618234555 24.74964008980137 24.983186293098335 92-93 24.98874293195694 25.368000778712656 25.074323936235025 24.568932353095374 94-95 24.85251425606805 25.31528681538599 24.831373611375902 25.00082531717006 96-97 24.663870700995886 25.535904665928477 24.717522733111057 25.082701899964583 98-99 24.924630517929682 25.127455833737365 25.000970153732073 24.94694349460088 100-101 25.041721288441327 25.30136961945001 24.78743607672839 24.86947301538028 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 19722.0 1 10503.5 2 1029.5 3 673.0 4 539.0 5 496.0 6 486.0 7 482.0 8 469.0 9 483.5 10 513.5 11 576.5 12 662.0 13 763.5 14 880.5 15 978.0 16 1138.0 17 1376.5 18 1698.0 19 2093.0 20 2615.0 21 3373.5 22 4452.5 23 6160.0 24 8371.5 25 11570.0 26 16239.0 27 22576.5 28 31470.0 29 43321.5 30 58994.0 31 79071.0 32 104342.0 33 137302.0 34 181346.5 35 240953.5 36 313940.5 37 410644.0 38 509127.0 39 590808.0 40 676270.5 41 757732.0 42 826637.0 43 886442.5 44 946583.0 45 956887.0 46 930642.0 47 936340.0 48 893519.5 49 835231.0 50 842691.5 51 799456.0 52 724631.5 53 681752.5 54 645393.0 55 635380.0 56 652829.0 57 656457.5 58 610850.0 59 521394.0 60 466410.5 61 429428.5 62 373434.0 63 339078.5 64 299507.5 65 275004.5 66 270495.0 67 266391.0 68 247719.5 69 209854.5 70 179854.0 71 156624.5 72 126300.0 73 100840.5 74 100302.5 75 90585.0 76 59160.0 77 36685.0 78 28047.5 79 31366.5 80 29426.0 81 18833.5 82 8396.5 83 2052.5 84 1257.5 85 923.0 86 749.5 87 614.0 88 525.0 89 433.0 90 341.5 91 292.5 92 246.5 93 180.5 94 132.0 95 102.0 96 71.0 97 53.0 98 32.5 99 16.0 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12963958586642116 2 0.11536873157528382 3 0.11161112717364122 4 0.12422434622933334 5 0.12623942302498237 6 0.11457789212333289 7 0.11633382378783412 8 0.1153285193997609 9 0.11307663757047685 10-11 0.10078958393847073 12-13 0.11066837505860366 14-15 0.14498499884792118 16-17 0.15581771213184803 18-19 0.14478393797030653 20-21 0.16850018548982965 22-23 0.15089842265954304 24-25 0.13723075100124965 26-27 0.14361108285088775 28-29 0.13262645690387428 30-31 0.10199594920415861 32-33 0.10130340618126371 34-35 0.125012951671533 36-37 0.12433381270714576 38-39 0.1329660263860679 40-41 0.16946304369262868 42-43 0.13686213939228947 44-45 0.13756361845418946 46-47 0.15832650508252857 48-49 0.18508770789303794 50-51 0.15665099776907318 52-53 0.1153017112827456 54-55 0.1173435961954099 56-57 0.1131503598922689 58-59 0.1151319265416488 60-61 0.11676722167958124 62-63 0.1020048852431637 64-65 0.11344524917943705 66-67 0.13425951803205546 68-69 0.12626399713224637 70-71 0.11554521834563447 72-73 0.12217352527766395 74-75 0.16732062834115707 76-77 0.17762834933353458 78-79 0.17427063267736997 80-81 0.14760996030566792 82-83 0.12923969812094313 84-85 0.134639299689772 86-87 0.1258685774062709 88-89 0.12991213505607654 90-91 0.14278003122341387 92-93 0.11812103158885319 94-95 0.11723636372734875 96-97 0.12085322551466111 98-99 0.11874432030945861 100-101 0.11120230338915811 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.2381281E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.8481529809262 #Duplication Level Percentage of deduplicated Percentage of total 1 67.49902703004342 27.572105821868863 2 17.382424133122175 14.200798403382361 3 6.131614712959811 7.513954074452409 4 2.999101547851557 4.900310353278918 5 1.7882651539146124 3.6523664288781807 6 0.9856556659549 2.415732805766547 7 0.6306644999460662 1.8033035981405365 8 0.5122544372646018 1.6739718094834173 9 0.3175136706999011 1.1672862294856485 >10 1.5723320261495393 11.097757969156463 >50 0.08256651905103148 2.350886691989121 >100 0.07452709073305276 6.634821070634119 >500 0.012246486928276009 3.4551045747197806 >1k 0.011017265747321488 8.606291062742061 >5k 6.024975546470668E-4 1.6093244902288368 >10k+ 1.8726207914722705E-4 1.345984615792581 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 66111 0.29538523733293015 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.31277923725634826 1.787207801019075E-5 4.468019502547687E-6 2 0.3134583762207355 2.2340097512738437E-5 4.468019502547687E-6 3 0.3135656086887967 2.2340097512738437E-5 4.468019502547687E-6 4 0.3138694340149699 2.2340097512738437E-5 4.468019502547687E-6 5 0.3139811345025336 2.2340097512738437E-5 4.468019502547687E-6 6 0.3141866633996508 2.6808117015286124E-5 8.936039005095375E-6 7 0.3143385760627374 2.6808117015286124E-5 8.936039005095375E-6 8 0.31455750901836227 4.468019502547687E-5 8.936039005095375E-6 9 0.3147317617789616 5.808425353311993E-5 8.936039005095375E-6 10-11 0.3151003733879218 7.372232179203683E-5 1.3404058507643062E-5 12-13 0.3154980271236486 9.606241930477528E-5 2.2340097512738437E-5 14-15 0.31596940318116734 1.0499845830987066E-4 2.9042126766559968E-5 16-17 0.3164430132484374 1.2063652656878754E-4 3.127613651783381E-5 18-19 0.31681832688665135 1.2733855582260908E-4 3.57441560203815E-5 20-21 0.3175510820850692 1.385086045789783E-4 3.57441560203815E-5 22-23 0.31796213987930355 1.586146923404429E-4 4.244618527420303E-5 24-25 0.31830170936149726 1.6978474109681212E-4 4.691420477675072E-5 26-27 0.31867702299971123 1.787207801019075E-4 4.914821452802456E-5 28-29 0.31910148485245327 1.9212483860955054E-4 4.914821452802456E-5 30-31 0.3194187142371342 2.14464936122289E-4 5.1382224279298404E-5 32-33 0.31982977203136853 2.3233701413247974E-4 7.1488312040763E-5 34-35 0.32017157552331343 2.412730531375751E-4 7.819034129458453E-5 36-37 0.3205558252005325 2.502090921426705E-4 8.042435104585837E-5 38-39 0.3209981591312847 2.680811701528612E-4 8.042435104585837E-5 40-41 0.32142485499377804 2.792512189092304E-4 9.382840955350143E-5 42-43 0.32191186911955577 2.9488928716814734E-4 1.2510454607133525E-4 44-45 0.32242792537209997 3.082933456757904E-4 1.2510454607133525E-4 46-47 0.32293057756613663 3.149953749296119E-4 1.3404058507643062E-4 48-49 0.32346897391619367 3.28399433437255E-4 1.385086045789783E-4 50-51 0.32427545143640346 3.440375016961719E-4 1.385086045789783E-4 52-53 0.3251556512784054 3.4627151144744575E-4 1.385086045789783E-4 54-55 0.32600904300339195 3.6190957970636266E-4 1.4521063383279983E-4 56-57 0.3269495611086783 3.775476479652795E-4 1.5191266308662137E-4 58-59 0.3280419918770512 3.8871769672164876E-4 1.541466728378952E-4 60-61 0.32909197646014987 4.1105779423438723E-4 1.5638068258916904E-4 62-63 0.33051727468146264 4.5350397950859024E-4 1.6084870209171674E-4 64-65 0.3320386353220801 4.6020600876241175E-4 1.6308271184299058E-4 66-67 0.3337744608988199 4.6020600876241175E-4 1.6531672159426442E-4 68-69 0.335358373812473 4.6914204776750716E-4 1.6755073134553825E-4 70-71 0.3376951480123055 4.892481355289717E-4 1.7201875084808596E-4 72-73 0.3399492638513408 4.914821452802455E-4 1.787207801019075E-4 74-75 0.3429584749863066 5.071202135391625E-4 1.8095478985318133E-4 76-77 0.34599896225779037 5.205242720468055E-4 1.8765681910700287E-4 78-79 0.34990401130301707 5.27226301300627E-4 1.943588483608244E-4 80-81 0.35484340686308347 5.406303598082702E-4 2.0329488736591975E-4 82-83 0.36136448132705185 5.540344183159132E-4 2.0552889711719362E-4 84-85 0.3698715904599026 5.585024378184608E-4 2.14464936122289E-4 86-87 0.3802083535790467 5.696724865748301E-4 2.1893295562483667E-4 88-89 0.39315444008767864 5.920125840875685E-4 2.1893295562483667E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGG 21375 0.0 32.973255 1 TCGGAAG 22690 0.0 31.962414 4 CCGTATC 12445 0.0 31.42144 48-49 ATCGGAA 23955 0.0 31.305523 3 CGCGTAT 6690 0.0 30.881414 1 AAGAGCG 23635 0.0 30.338913 8 GAGTGTA 11655 0.0 30.193485 28-29 GCCGTAT 11945 0.0 29.932396 48-49 TCGTGTA 13060 0.0 29.283323 14-15 CGTGTAG 12390 0.0 28.62392 16-17 GTAGGGA 13640 0.0 28.52579 18-19 CGCCGTA 13815 0.0 28.428564 46-47 TGTAGGG 12985 0.0 27.568268 18-19 AGAGCGT 26025 0.0 27.231184 9 CGTATCA 13005 0.0 27.117994 50-51 GTCGTGT 13135 0.0 27.108734 14-15 ACATGCG 7700 0.0 27.077517 4 GATCGGA 27920 0.0 26.825476 2 CATGCGT 7985 0.0 26.109665 5 GCGTATT 8110 0.0 25.708387 2 >>END_MODULE