FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n02 m48-2.3420000000cc9f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n02 m48-2.3420000000cc9f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22381281
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC661110.29538523733293015Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG213750.032.9732551
TCGGAAG226900.031.9624144
CCGTATC124450.031.4214448-49
ATCGGAA239550.031.3055233
CGCGTAT66900.030.8814141
AAGAGCG236350.030.3389138
GAGTGTA116550.030.19348528-29
GCCGTAT119450.029.93239648-49
TCGTGTA130600.029.28332314-15
CGTGTAG123900.028.6239216-17
GTAGGGA136400.028.5257918-19
CGCCGTA138150.028.42856446-47
TGTAGGG129850.027.56826818-19
AGAGCGT260250.027.2311849
CGTATCA130050.027.11799450-51
GTCGTGT131350.027.10873414-15
ACATGCG77000.027.0775174
GATCGGA279200.026.8254762
CATGCGT79850.026.1096655
GCGTATT81100.025.7083872
Produced by FastQC (version 0.11.2)