FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n02 m12-1.3420000000cb69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n02 m12-1.3420000000cb69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20788236
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT36200.025.7149161
ACATGCG54000.022.163864
CATGCGT57450.021.3277115
GCGTATT45000.020.0528242
TAAGTCG44250.019.1068579
TCCGTAC55650.018.0066055
GTCCGTA60550.016.6287784
ATGCGTG97750.015.1559946
CTGACAT129750.014.861281
CCGTACC72850.014.6655916
TTAAAAA215850.014.35507156-57
AAAGGGC122000.014.3259172
CGCGTGT28350.014.239823
TTTAAAA129750.013.8893356-57
TTAAGTC64250.013.5257448
CGTGCGA88300.013.4481299
TAAAAAA224000.013.28123558-59
CGCAAAT61500.012.9739351
TGCGTGC116650.012.90381057
GACATAG99400.012.4242329
Produced by FastQC (version 0.11.2)