##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l05n02 kx48-2.3420000000cd31.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16624912 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39342650355081 33.0 33.0 33.0 33.0 33.0 2 32.37617949496514 33.0 33.0 33.0 33.0 33.0 3 32.372565400646934 33.0 33.0 33.0 33.0 33.0 4 36.20530478597421 37.0 37.0 37.0 37.0 37.0 5 36.198962617065284 37.0 37.0 37.0 37.0 37.0 6 36.179665131460546 37.0 37.0 37.0 37.0 37.0 7 36.23618561108775 37.0 37.0 37.0 37.0 37.0 8 36.212757577303265 37.0 37.0 37.0 37.0 37.0 9 36.20913181375035 37.0 37.0 37.0 37.0 37.0 10-11 36.19611911329215 37.0 37.0 37.0 37.0 37.0 12-13 36.19079592722055 37.0 37.0 37.0 37.0 37.0 14-15 38.38879661438208 40.0 37.0 40.0 37.0 40.0 16-17 38.344007505122434 40.0 37.0 40.0 37.0 40.0 18-19 38.34719780772373 40.0 37.0 40.0 37.0 40.0 20-21 38.31614693659732 40.0 37.0 40.0 37.0 40.0 22-23 38.28481868054399 40.0 37.0 40.0 37.0 40.0 24-25 38.24527693740575 40.0 37.0 40.0 37.0 40.0 26-27 38.20165276664322 40.0 37.0 40.0 37.0 40.0 28-29 38.15535188998294 40.0 37.0 40.0 37.0 40.0 30-31 38.11303620734955 40.0 37.0 40.0 37.0 40.0 32-33 38.03898561989381 40.0 37.0 40.0 35.0 40.0 34-35 37.97408190792228 40.0 37.0 40.0 33.0 40.0 36-37 37.90126495105658 40.0 37.0 40.0 33.0 40.0 38-39 37.80901919962042 40.0 37.0 40.0 33.0 40.0 40-41 37.70479368552447 40.0 37.0 40.0 33.0 40.0 42-43 37.61444054561011 40.0 37.0 40.0 33.0 40.0 44-45 37.502309997189755 40.0 37.0 40.0 33.0 40.0 46-47 37.36620611886547 40.0 37.0 40.0 33.0 40.0 48-49 37.239566501164035 40.0 37.0 40.0 33.0 40.0 50-51 35.79080268214352 38.5 35.0 38.5 30.0 38.5 52-53 36.13323580299252 37.0 37.0 38.5 33.0 40.0 54-55 36.85594648561147 37.0 37.0 40.0 33.0 40.0 56-57 36.82471979400553 37.0 37.0 40.0 33.0 40.0 58-59 36.702289702345496 37.0 37.0 40.0 33.0 40.0 60-61 36.52589559571804 37.0 37.0 40.0 33.0 40.0 62-63 36.334600598186626 37.0 37.0 40.0 33.0 40.0 64-65 36.14784929387897 37.0 37.0 40.0 33.0 40.0 66-67 35.93763034655461 37.0 37.0 40.0 33.0 40.0 68-69 35.72816529795767 37.0 37.0 37.0 33.0 40.0 70-71 35.52284676754981 37.0 37.0 37.0 33.0 40.0 72-73 35.306606976325654 37.0 37.0 37.0 33.0 40.0 74-75 35.08507127736977 37.0 35.0 37.0 33.0 38.5 76-77 34.883320314718055 37.0 33.0 37.0 30.0 37.0 78-79 34.69882938327734 37.0 33.0 37.0 27.0 37.0 80-81 34.55928690028554 37.0 33.0 37.0 27.0 37.0 82-83 34.45004147991881 37.0 33.0 37.0 27.0 37.0 84-85 34.352580482832025 37.0 33.0 37.0 27.0 37.0 86-87 34.261517053443654 37.0 33.0 37.0 27.0 37.0 88-89 34.184578841680484 37.0 33.0 37.0 27.0 37.0 90-91 34.08374964631392 37.0 33.0 37.0 27.0 37.0 92-93 34.0174398517117 37.0 33.0 37.0 27.0 37.0 94-95 33.948037830215284 37.0 33.0 37.0 27.0 37.0 96-97 33.865067706824554 37.0 33.0 37.0 27.0 37.0 98-99 33.78782774910327 37.0 33.0 37.0 27.0 37.0 100-101 32.06957721039365 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1394934201317355 2306 2 0.25179591096669185 2306 3 0.28673835735799 2306 4 0.3861176596536282 2306 5 0.41246744223006715 2306 6 0.3914405292080261 2306 7 0.38746564606415745 2306 8 0.42201034039896257 2306 9 0.42962808422520027 2306 10-11 0.4267184946865683 2306 12-13 0.4237875959512465 2306 14-15 0.7294302485434159 2306 16-17 0.6934264445807088 2306 18-19 0.7044499148409784 2306 20-21 0.7238054387839412 2306 22-23 0.7320063992988892 2306 24-25 0.702714591363403 2306 26-27 0.7460787877887114 2306 28-29 0.6923839878580225 2306 30-31 0.7348548559548149 2306 32-33 0.7743209640049074 2306 34-35 0.7799182907053961 2306 36-37 0.7197106920597705 2306 38-39 0.7466402313427949 2306 40-41 0.8095611866708978 2306 42-43 0.7846084215890343 2306 44-45 0.7845727579353081 2306 46-47 0.8587436157466897 2306 48-49 0.8057167188266732 2306 50-51 0.664613393459625 2306 52-53 0.758107150219864 2306 54-55 0.8040640875157195 2306 56-57 0.7799454276815752 2306 58-59 0.8166173344922001 2306 60-61 0.7720145967872725 2306 62-63 0.7934840415956472 2306 64-65 0.8444431763066262 2306 66-67 0.8383862731230778 2306 68-69 0.8516130807944222 2306 70-71 0.8144866024489517 2306 72-73 0.8171325208441402 2306 74-75 0.8426701854080036 2306 76-77 0.8795734068995884 2306 78-79 0.8757798806836234 2306 80-81 0.7793659987546562 2306 82-83 0.856906345375883 2306 84-85 0.8667594182029745 2306 86-87 0.8857307517048127 2306 88-89 0.7796497339739901 2306 90-91 0.888907732143231 2306 92-93 0.9331032778304476 2306 94-95 0.9046129374954077 2306 96-97 0.9323436586384659 2306 98-99 0.8646968076052133 2306 100-101 0.6080746947832409 2307 1 -0.006605829245167172 2307 2 0.13116627936172165 2307 3 0.18206598822818165 2307 4 0.22253565925613117 2307 5 0.2632615605347013 2307 6 0.2742573478079109 2307 7 0.1711793336368217 2307 8 0.25026998739321016 2307 9 0.22433654764650157 2307 10-11 0.2535490647975891 2307 12-13 0.26759947723897426 2307 14-15 0.4560591777152112 2307 16-17 0.46802109282754856 2307 18-19 0.41736488401306104 2307 20-21 0.45752226755000436 2307 22-23 0.4403705171542285 2307 24-25 0.44237066208393117 2307 26-27 0.4467938914860383 2307 28-29 0.4446871452051937 2307 30-31 0.45721044254786136 2307 32-33 0.4325131996692235 2307 34-35 0.4787608772171055 2307 36-37 0.4742247058299114 2307 38-39 0.42508094686930775 2307 40-41 0.4827691175866917 2307 42-43 0.44334807559619094 2307 44-45 0.43644812994305937 2307 46-47 0.45115671607949537 2307 48-49 0.4215714744236223 2307 50-51 0.32823812494326887 2307 52-53 0.3155748553311568 2307 54-55 0.4883931485858213 2307 56-57 0.44122056241106833 2307 58-59 0.5315353069502677 2307 60-61 0.5338377927912035 2307 62-63 0.5360878694081634 2307 64-65 0.6060070378540416 2307 66-67 0.5633985241516797 2307 68-69 0.5579537048440315 2307 70-71 0.5229984073981484 2307 72-73 0.5258559317042 2307 74-75 0.5125771399184842 2307 76-77 0.5193239735895006 2307 78-79 0.5142646676261577 2307 80-81 0.5273096541396569 2307 82-83 0.5124811803523315 2307 84-85 0.5244663855776963 2307 86-87 0.5878430571037185 2307 88-89 0.5532800456733042 2307 90-91 0.5192974255175571 2307 92-93 0.46578876385018475 2307 94-95 0.5395580959458925 2307 96-97 0.4262470809370953 2307 98-99 0.39764200242298386 2307 100-101 0.42210166607208066 2201 1 0.21695975988875205 2201 2 0.24686548602423386 2201 3 0.28051200714032376 2201 4 0.31951462220254 2201 5 0.35389730860043755 2201 6 0.37485219973322614 2201 7 0.3358683802634772 2201 8 0.3871999225774587 2201 9 0.3958474448308138 2201 10-11 0.39044822196369466 2201 12-13 0.40419355546442404 2201 14-15 0.6152239571284284 2201 16-17 0.6646483042305746 2201 18-19 0.6654837520251249 2201 20-21 0.6584668277518091 2201 22-23 0.6285463282896657 2201 24-25 0.6656930911667231 2201 26-27 0.6326396405418038 2201 28-29 0.7082650368379007 2201 30-31 0.6489952242604318 2201 32-33 0.6893605991266654 2201 34-35 0.709240086524737 2201 36-37 0.6816955605303932 2201 38-39 0.6625927478646005 2201 40-41 0.7426462770896123 2201 42-43 0.7108697223319211 2201 44-45 0.6456671067288582 2201 46-47 0.7479958644155218 2201 48-49 0.7706059042128999 2201 50-51 0.7153052388107 2201 52-53 0.7489615479804854 2201 54-55 0.7902613380018622 2201 56-57 0.722072868708679 2201 58-59 0.7197984689556378 2201 60-61 0.7325216160406427 2201 62-63 0.7960325438039035 2201 64-65 0.8069836500920289 2201 66-67 0.8348917003900951 2201 68-69 0.7762842087015613 2201 70-71 0.7917216668278471 2201 72-73 0.7673034606825055 2201 74-75 0.7706804429779837 2201 76-77 0.8194819832740023 2201 78-79 0.8022826521163893 2201 80-81 0.771838476386904 2201 82-83 0.7964540406394391 2201 84-85 0.7786704677218026 2201 86-87 0.8009590454318385 2201 88-89 0.7670183756882025 2201 90-91 0.812502784114848 2201 92-93 0.7518141196152826 2201 94-95 0.8258439642103994 2201 96-97 0.8259065407524773 2201 98-99 0.7598454608143044 2201 100-101 0.6312988111879463 1101 1 0.2508187133525368 1101 2 0.2511760708113897 1101 3 0.2500933568739896 1101 4 0.32251993895991404 1101 5 0.3470692342786492 1101 6 0.30875241590193525 1101 7 0.3787483777067777 1101 8 0.3569292397329846 1101 9 0.36453511720191045 1101 10-11 0.3647526071282954 1101 12-13 0.3873230349041421 1101 14-15 0.577031850005163 1101 16-17 0.6208855298114955 1101 18-19 0.5868664873324718 1101 20-21 0.600912153344737 1101 22-23 0.599470807072926 1101 24-25 0.6124437093832995 1101 26-27 0.6172078056585946 1101 28-29 0.6287931685899508 1101 30-31 0.6281316193230211 1101 32-33 0.6412361411415972 1101 34-35 0.6150199728899324 1101 36-37 0.6599567199502232 1101 38-39 0.6270698757145468 1101 40-41 0.6516145964200533 1101 42-43 0.6043545383577182 1101 44-45 0.6066494110717926 1101 46-47 0.6617416402583913 1101 48-49 0.6490241107901227 1101 50-51 0.6653885455496891 1101 52-53 0.6685979501801498 1101 54-55 0.6058688734488058 1101 56-57 0.6034825001140476 1101 58-59 0.6529496969823398 1101 60-61 0.6787089643034747 1101 62-63 0.675341381074567 1101 64-65 0.6418182109598405 1101 66-67 0.6620317678907597 1101 68-69 0.674336463200703 1101 70-71 0.728250401386731 1101 72-73 0.6855751609160166 1101 74-75 0.7289012927233287 1101 76-77 0.7722415481282141 1101 78-79 0.7782893866296732 1101 80-81 0.7412110576201059 1101 82-83 0.6470213105867728 1101 84-85 0.7041206092276369 1101 86-87 0.7183312983745012 1101 88-89 0.7712009856712285 1101 90-91 0.7977644468341296 1101 92-93 0.81576241112041 1101 94-95 0.7660569817301521 1101 96-97 0.7760111190487109 1101 98-99 0.7495194354638741 1101 100-101 0.6873536019270503 2304 1 0.1341709450934374 2304 2 0.1821737223525659 2304 3 0.2255012472169895 2304 4 0.276804230467782 2304 5 0.3034176926308447 2304 6 0.3128885777407717 2304 7 0.32196032569189015 2304 8 0.31122049189152534 2304 9 0.33905234030959974 2304 10-11 0.3175701291198578 2304 12-13 0.33806102980243935 2304 14-15 0.5221825216542442 2304 16-17 0.5738397862466371 2304 18-19 0.5541094724665214 2304 20-21 0.5351791385131577 2304 22-23 0.5498846256733643 2304 24-25 0.5942621903198173 2304 26-27 0.5598511628067229 2304 28-29 0.5977075670587126 2304 30-31 0.6000813468737931 2304 32-33 0.6256273398442644 2304 34-35 0.6131646186810116 2304 36-37 0.58992436042913 2304 38-39 0.6012507121247879 2304 40-41 0.5893829059228253 2304 42-43 0.581242011046804 2304 44-45 0.6652816465184301 2304 46-47 0.6561679594713965 2304 48-49 0.6121849359286458 2304 50-51 0.5897802056794745 2304 52-53 0.6629502909643534 2304 54-55 0.7201935985571524 2304 56-57 0.696966300407901 2304 58-59 0.6928158805041136 2304 60-61 0.6432910358897814 2304 62-63 0.6782664895268056 2304 64-65 0.672252943458318 2304 66-67 0.6760289444690812 2304 68-69 0.6833555008350487 2304 70-71 0.6914311342615918 2304 72-73 0.6539281701705448 2304 74-75 0.6616845198437389 2304 76-77 0.6953279554425436 2304 78-79 0.7425160106269288 2304 80-81 0.6962800299572152 2304 82-83 0.7737289265341687 2304 84-85 0.7217565537303088 2304 86-87 0.6298531732485486 2304 88-89 0.7633129298434795 2304 90-91 0.7897339317546468 2304 92-93 0.768617645242621 2304 94-95 0.7339958598090277 2304 96-97 0.7532595231629102 2304 98-99 0.6745845678075924 2304 100-101 0.6913975678477584 2202 1 0.15803259567417172 2202 2 0.24102917918395406 2202 3 0.24811345455373868 2202 4 0.3261716267234007 2202 5 0.36670460138427785 2202 6 0.3655567749905302 2202 7 0.37641720719329896 2202 8 0.4067273310928954 2202 9 0.404665787871167 2202 10-11 0.40017067876220125 2202 12-13 0.40501158414544847 2202 14-15 0.6367167807003753 2202 16-17 0.6429890972733219 2202 18-19 0.6041964442533327 2202 20-21 0.6576349895568541 2202 22-23 0.6663364646813648 2202 24-25 0.6956118518590557 2202 26-27 0.6887665045939286 2202 28-29 0.7233067388930863 2202 30-31 0.6986341676691694 2202 32-33 0.704854078563919 2202 34-35 0.6739072641696637 2202 36-37 0.7570113504167963 2202 38-39 0.7953532341119143 2202 40-41 0.7724181404553505 2202 42-43 0.7965953190706259 2202 44-45 0.7766322301951973 2202 46-47 0.7903244259135747 2202 48-49 0.73112740909027 2202 50-51 0.672012940151447 2202 52-53 0.7142204955771163 2202 54-55 0.8214109720056513 2202 56-57 0.8416278852295491 2202 58-59 0.8536534418497794 2202 60-61 0.8432034372500539 2202 62-63 0.8358871080498105 2202 64-65 0.8300702758029601 2202 66-67 0.8571344970909678 2202 68-69 0.8641638219255725 2202 70-71 0.8221585677260208 2202 72-73 0.8471326626617142 2202 74-75 0.8408212159347386 2202 76-77 0.841020801817649 2202 78-79 0.859078065128088 2202 80-81 0.8568473955693108 2202 82-83 0.9043076041128586 2202 84-85 0.8651089156634484 2202 86-87 0.9117016159054714 2202 88-89 0.8855239321358681 2202 90-91 0.8653697840721151 2202 92-93 0.8899618279398354 2202 94-95 0.9224740066394546 2202 96-97 0.9240221058193683 2202 98-99 0.9689594345275978 2202 100-101 1.086954092149199 1102 1 0.04696259136525782 1102 2 0.0832434319299864 1102 3 0.11534104571592962 1102 4 0.1534230712248359 1102 5 0.12680089135820793 1102 6 0.136062729315249 1102 7 0.12578231949186147 1102 8 0.15772889456732742 1102 9 0.1841942884251182 1102 10-11 0.16340295258208215 1102 12-13 0.1432736970401649 1102 14-15 0.2497775106410316 1102 16-17 0.32722660505883994 1102 18-19 0.24035391487147706 1102 20-21 0.2821759977071565 1102 22-23 0.29613452369914484 1102 24-25 0.30427045023170507 1102 26-27 0.2775890686641205 1102 28-29 0.2946707747598083 1102 30-31 0.32788920704673785 1102 32-33 0.34147744793694557 1102 34-35 0.37485086439809123 1102 36-37 0.35204782061838813 1102 38-39 0.35514867671933104 1102 40-41 0.32818741114390804 1102 42-43 0.320177808077311 1102 44-45 0.3379176291420194 1102 46-47 0.3850006605645646 1102 48-49 0.34650385010714047 1102 50-51 0.2744338502381254 1102 52-53 0.3036976600294494 1102 54-55 0.3871113767428298 1102 56-57 0.41564364377549623 1102 58-59 0.4319989650203482 1102 60-61 0.4239789029969785 1102 62-63 0.3475054559161066 1102 64-65 0.39375624742307025 1102 66-67 0.43170380986310875 1102 68-69 0.39851983229205246 1102 70-71 0.4179541192509788 1102 72-73 0.4060122683105334 1102 74-75 0.4046635947336128 1102 76-77 0.44968862806450716 1102 78-79 0.4456716028932064 1102 80-81 0.43868804920994364 1102 82-83 0.3807444979977035 1102 84-85 0.39978442328488484 1102 86-87 0.46314954092680694 1102 88-89 0.5132122303003257 1102 90-91 0.5128356816322821 1102 92-93 0.49368112419514176 1102 94-95 0.4678482419268235 1102 96-97 0.5233344232281709 1102 98-99 0.5595933375905915 1102 100-101 0.545050539376561 2305 1 0.11040193416736344 2305 2 0.14011056103546338 2305 3 0.1216972038608759 2305 4 0.18597945601339205 2305 5 0.1411604856741988 2305 6 0.19427335636057563 2305 7 0.20271154754427556 2305 8 0.21908319370111684 2305 9 0.16553986224212736 2305 10-11 0.17126169031462268 2305 12-13 0.19713260681228206 2305 14-15 0.2991105722211387 2305 16-17 0.3247466985139269 2305 18-19 0.32041238056759624 2305 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1213 96-97 -1.2655604862256453 1213 98-99 -1.2931686575678683 1213 100-101 -1.1492807434462158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16402.0 3 1143.0 4 649.0 5 593.0 6 587.0 7 807.0 8 1375.0 9 3234.0 10 7665.0 11 12655.0 12 15489.0 13 18444.0 14 21101.0 15 23617.0 16 27113.0 17 31211.0 18 35392.0 19 39574.0 20 44585.0 21 50064.0 22 56011.0 23 63088.0 24 72111.0 25 82584.0 26 95662.0 27 112159.0 28 132291.0 29 160585.0 30 198416.0 31 253369.0 32 331688.0 33 447023.0 34 621010.0 35 948858.0 36 1967701.0 37 5326048.0 38 5398760.0 39 5848.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.757759873645856 13.671678222870225 11.35284673850537 54.21771516497855 2 21.418748372197356 18.46688917693782 40.952482464895915 19.161879985968906 3 23.464109411260157 23.933460247080763 25.6816254594421 26.920804882216988 4 26.40842052823249 29.006160931734705 18.828870709445752 25.756547830587056 5 27.964063792423723 30.658454257166568 22.007967006460742 19.369514943948975 6 21.03124575641179 33.416112974066195 23.458492400458027 22.094148869063982 7 19.086792671443018 16.038548772908065 41.57064612270552 23.304012432943395 8 20.855848124896497 21.11545097330744 28.40260150851362 29.626099393282445 9 21.2910574422556 20.857465198897636 31.585198379757383 26.266278979089382 10-11 24.643186144717216 28.89883869859074 21.71127347264679 24.74670168404525 12-13 22.700201893623994 23.370194864757167 28.344238941126576 25.585364300492262 14-15 23.350441584063763 25.096833088672305 26.761548099361963 24.791177227901965 16-17 24.478988836287286 25.184590663616095 25.647314755309665 24.68910574478695 18-19 24.179549913233558 25.539727151945122 25.645375114873374 24.635347819947945 20-21 24.04657997328547 25.60268412994403 25.922673434009997 24.4280624627605 22-23 23.89627115599324 25.608789920816577 25.8728280297748 24.622110893415382 24-25 23.9412742803069 25.59582972424046 25.825435341604724 24.637460653847914 26-27 23.77828996556617 25.66187417761553 26.03040964233971 24.52942621447859 28-29 24.04113374067144 25.54818837535457 25.609605301237764 24.801072582736225 30-31 24.0170117784272 25.47262282629868 25.694565328980385 24.81580006629373 32-33 24.050293819961766 25.661470861304593 25.647050138864934 24.641185179868707 34-35 24.033381024181644 25.666317798354676 25.440509982917536 24.859791194546137 36-37 23.949549744441732 25.56180088250969 25.62114416940688 24.867505203641695 38-39 24.05630423960727 25.643746986583913 25.79962699141442 24.500321782394394 40-41 24.2850491411835 25.488553607173902 25.63819068378902 24.588206567853575 42-43 24.03814499733304 25.460420842875347 25.89977147346385 24.60166268632776 44-45 24.104207981800823 25.520082799323557 25.89811401808498 24.477595200790645 46-47 24.275683301470423 25.473119512338094 25.608446269298106 24.642750916893377 48-49 23.970367618853118 25.590721800791798 25.61347371039594 24.82543686995914 50-51 23.866856454206157 25.629887669746065 26.057674743983224 24.445581132064557 52-53 24.200232576072523 25.579282043246526 25.691664805614465 24.528820575066483 54-55 24.03844017150764 25.55854734601749 25.671745182798816 24.731267299676052 56-57 23.884682724265048 25.563281189992924 26.037636556243392 24.514399529498636 58-59 24.279481197195178 25.470359928042214 25.605405734498216 24.64475314026439 60-61 24.105914353546957 25.340581544696335 25.799231920281194 24.754272181475514 62-63 24.01851304590978 25.538515738593848 25.95893753141565 24.48403368408072 64-65 24.137635857484597 25.58066109698992 25.569695214403932 24.712007831121554 66-67 24.084594632524205 25.470522972620536 25.538521399372026 24.906360995483233 68-69 24.121427758582335 25.595071306532645 25.743253239665453 24.540247695219563 70-71 24.26868370413631 25.55212152183645 25.4831541315336 24.696040642493642 72-73 24.180419377468418 25.58305781539137 25.648873773832616 24.587649033307596 74-75 24.384061109093537 25.517059275501396 25.704084769707425 24.39479484569764 76-77 24.552513534714343 25.53605880735916 25.47325855230167 24.438169105624823 78-79 24.279790397711448 25.516760000390455 25.58423408240129 24.619215519496805 80-81 24.350557074991546 25.60673928394024 25.5305124714029 24.512191169665318 82-83 24.320078460841987 25.640693226160373 25.384788448828516 24.654439864169124 84-85 24.240356555736348 25.50622844094744 25.465219580208643 24.788195423107574 86-87 24.265358786880668 25.813681033258757 25.559537032478218 24.36142314738235 88-89 24.429831636980786 25.705769258972584 25.387802722220698 24.476596381825935 90-91 24.47878621972818 25.49524431193348 25.446558147168112 24.579411321170237 92-93 24.369951286890103 25.72745897412712 25.69094348795671 24.211646251026067 94-95 24.35277094131525 25.627214489933518 25.497043010817443 24.52297155793379 96-97 24.172839512120827 25.742968213682644 25.449343140669328 24.6348491335272 98-99 24.411548411265247 25.59097037643696 25.611343499680416 24.386137712617373 100-101 24.55170479357802 25.6172896006544 25.400085075285123 24.43092053048246 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14696.0 1 7847.0 2 790.5 3 503.0 4 402.5 5 378.0 6 349.5 7 328.5 8 351.0 9 375.5 10 418.5 11 476.5 12 524.0 13 626.0 14 744.5 15 854.0 16 987.5 17 1204.5 18 1555.0 19 1940.5 20 2471.5 21 3355.5 22 4715.5 23 6682.5 24 9523.5 25 13609.0 26 19384.5 27 27120.0 28 37044.5 29 49784.0 30 66236.0 31 86233.5 32 109507.0 33 138396.0 34 175363.5 35 223635.5 36 279725.0 37 349353.5 38 425981.5 39 497823.5 40 570770.0 41 635907.0 42 686211.0 43 724353.0 44 752399.5 45 746088.5 46 715096.0 47 694010.5 48 652415.5 49 598134.0 50 569378.0 51 530592.5 52 486064.5 53 458600.5 54 437276.0 55 422019.0 56 416011.0 57 410962.0 58 387030.0 59 345287.5 60 315641.0 61 296102.0 62 270164.5 63 246542.5 64 217170.0 65 195170.0 66 188769.0 67 181539.5 68 169639.0 69 148340.5 70 123982.0 71 102054.0 72 81151.5 73 68320.5 74 59473.5 75 48993.0 76 35199.5 77 21923.5 78 15752.5 79 15927.5 80 13512.5 81 8187.0 82 3882.0 83 1313.0 84 804.0 85 578.0 86 465.0 87 372.0 88 298.5 89 247.0 90 213.0 91 176.0 92 124.5 93 102.5 94 83.5 95 52.5 96 39.0 97 25.0 98 11.0 99 7.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1288427872580619 2 0.11390736985555172 3 0.11042464465375816 4 0.12296606442187483 5 0.12591946351355124 6 0.1141479726328777 7 0.1163976086008756 8 0.11526076047801036 9 0.11282465735758482 10-11 0.1009178274146654 12-13 0.11064419468806812 14-15 0.1446774575408279 16-17 0.155306085229203 18-19 0.14450602806198312 20-21 0.16812720572596113 22-23 0.1505902707936138 24-25 0.13683380700000097 26-27 0.14313459223122504 28-29 0.13231348232098913 30-31 0.10229828584957322 32-33 0.10145918366364887 34-35 0.12480066059898542 36-37 0.12436156053036551 38-39 0.13279468787564108 40-41 0.16896029284245234 42-43 0.1364368124174131 44-45 0.13740824613086675 46-47 0.15758880407908324 48-49 0.18466563913240563 50-51 0.1563918052618865 52-53 0.11526376801272692 54-55 0.11711941693285353 56-57 0.1130622526001942 58-59 0.11467729874299484 60-61 0.11635550311484355 62-63 0.10194339675301739 64-65 0.11345323211334893 66-67 0.1342683798867627 68-69 0.12630743549198936 70-71 0.11571790575492971 72-73 0.12196455536125546 74-75 0.16667456645785556 76-77 0.1770716139730544 78-79 0.1738956573123515 80-81 0.14709250791823741 82-83 0.12914654826443592 84-85 0.13475259297613124 86-87 0.12595254639543355 88-89 0.12988038673528016 90-91 0.14247594212829517 92-93 0.11806679036857459 94-95 0.11734197450188008 96-97 0.12068033803727804 98-99 0.11850589043719449 100-101 0.11094194062500902 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6624912E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.252255916738825 #Duplication Level Percentage of deduplicated Percentage of total 1 75.38126039300667 40.89603430167726 2 14.644790969344285 15.890258950320234 3 4.360312819777811 7.096704209268687 4 2.0359401070264114 4.41817374870198 5 0.9161164688133154 2.4850692557799534 6 0.6770999354225 2.2040519386649278 7 0.40958500689009275 1.555463742942238 8 0.26815063594639027 1.1638221540479863 9 0.1957510682373445 0.9557943344988675 >10 0.9844071135130121 9.453512715886424 >50 0.06325499843392915 2.4022049343987866 >100 0.0538549161954082 5.78728174152309 >500 0.0061012514069870875 2.25716224987551 >1k 0.0032572110418414525 3.0138423687284894 >5k 1.0599105410173928E-4 0.3412324541223705 >10k+ 1.111388978404298E-5 0.07939089956322928 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.010754944146471272 3.0075347165747402E-5 0.0 2 0.010803064701936467 3.0075347165747402E-5 0.0 3 0.011043667479262446 4.210548603204637E-5 0.0 4 0.011963973102534317 4.210548603204637E-5 0.0 5 0.012204575879860296 4.8120555465195846E-5 0.0 6 0.012896308864672487 6.616576376464429E-5 1.804520829944844E-5 7 0.013046685600501224 7.819590263094325E-5 1.804520829944844E-5 8 0.013389544558190743 8.421097206409273E-5 1.804520829944844E-5 9 0.0138948103905753 8.421097206409273E-5 3.0075347165747402E-5 10-11 0.014836168756863194 9.02260414972422E-5 3.609041659889688E-5 12-13 0.01582865521333286 9.624111093039169E-5 4.210548603204637E-5 14-15 0.01692039031544949 1.1428631922984014E-4 5.4135624898345325E-5 16-17 0.018105358993779935 1.4736920111216227E-4 6.0150694331494804E-5 18-19 0.019197094095896566 1.714294788447602E-4 8.421097206409272E-5 20-21 0.02028582166329662 1.9248222186078338E-4 9.02260414972422E-5 22-23 0.021230187564301093 2.165424995933813E-4 9.924864564696644E-5 24-25 0.022168538395872413 2.3458770789282974E-4 1.1428631922984014E-4 26-27 0.02303170085952936 2.4060277732597922E-4 1.2330892337956436E-4 28-29 0.023834712628854816 2.466178467591287E-4 1.3233152752928858E-4 30-31 0.0246106565857311 2.4962538147570347E-4 1.5939933997846125E-4 32-33 0.025395623146757106 2.5864798562542766E-4 1.804520829944844E-4 34-35 0.026114423944018472 2.6466305505857716E-4 2.2255756902653077E-4 36-37 0.026923450782777074 3.12783610523773E-4 2.2857263845968027E-4 38-39 0.027582100885706944 3.12783610523773E-4 2.3458770789282974E-4 40-41 0.028343007169000352 3.1579114524034774E-4 2.4060277732597922E-4 42-43 0.029115943591160064 3.3082881882322144E-4 2.6466305505857716E-4 44-45 0.029906925221619217 3.3684388825637094E-4 2.7067812449172666E-4 46-47 0.03078813289357562 3.3684388825637094E-4 2.7067812449172666E-4 48-49 0.031660317961382294 3.398514229729457E-4 2.857157980746003E-4 50-51 0.032610698931819906 3.578966312723941E-4 3.0075347165747405E-4 52-53 0.033494914138492884 3.7894937428841727E-4 3.097760758071983E-4 54-55 0.03448439306024598 3.9097951315471627E-4 3.3082881882322144E-4 56-57 0.035437781565400166 3.9398704787129096E-4 3.398514229729457E-4 58-59 0.03644530569545271 4.0000211730444046E-4 3.609041659889689E-4 60-61 0.037840801803943384 4.240623950370384E-4 3.729343048552678E-4 62-63 0.039224267773567766 4.4511513805306155E-4 3.8496444372156677E-4 64-65 0.04080623103448608 4.6316034635251E-4 4.030096520210152E-4 66-67 0.042361126482955216 4.691754157856595E-4 4.1503979088731416E-4 68-69 0.044087451410269124 4.8722062408510793E-4 4.240623950370384E-4 70-71 0.046024303767743246 5.142884365342806E-4 4.3007746447018785E-4 72-73 0.04821980411084281 5.233110406840048E-4 4.5714527691936054E-4 74-75 0.050770193550498194 5.383487142668785E-4 4.6616788106908474E-4 76-77 0.05361832892709447 5.714315961492007E-4 5.052658323845564E-4 78-79 0.057025865760973654 5.774466655823501E-4 5.263185754005796E-4 80-81 0.0611281431143816 5.954918738817986E-4 5.684240614326259E-4 82-83 0.06613869595219513 5.984994085983734E-4 6.436124293469945E-4 84-85 0.07327858336934355 6.015069433149481E-4 6.556425682132934E-4 86-87 0.08225908203303572 6.105295474646723E-4 6.646651723630176E-4 88-89 0.09274936312444843 6.345898251972702E-4 6.736877765127419E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAT 3730 0.0 27.50343 1 ACATGCG 5025 0.0 26.275557 4 CATGCGT 5455 0.0 24.202808 5 GCGTATT 4835 0.0 20.628237 2 TAAGTCG 4920 0.0 20.273443 9 GTCCGTA 4125 0.0 19.688625 4 CGCGTGT 2315 0.0 18.66954 3 ATGCGTG 7685 0.0 18.103218 6 GCGTGCG 7390 0.0 17.54226 8 CGTGCGA 7460 0.0 17.445564 9 TGCGTGC 8455 0.0 16.959827 7 TCCGTAC 4795 0.0 16.936485 5 CTGACAT 12125 0.0 15.668238 1 TTAAGTC 7180 0.0 14.285541 8 CCGTACC 6555 0.0 13.980305 6 CGTATTT 7980 0.0 13.867437 3 CGCGGAT 5080 0.0 13.743442 1 TGTCCGT 6105 0.0 13.692105 3 GTATTTA 8100 0.0 13.251548 4 GCGTGTA 3685 0.0 12.759714 4 >>END_MODULE