FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n02 kx48-2.3420000000cd31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n02 kx48-2.3420000000cd31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16624912
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT37300.027.503431
ACATGCG50250.026.2755574
CATGCGT54550.024.2028085
GCGTATT48350.020.6282372
TAAGTCG49200.020.2734439
GTCCGTA41250.019.6886254
CGCGTGT23150.018.669543
ATGCGTG76850.018.1032186
GCGTGCG73900.017.542268
CGTGCGA74600.017.4455649
TGCGTGC84550.016.9598277
TCCGTAC47950.016.9364855
CTGACAT121250.015.6682381
TTAAGTC71800.014.2855418
CCGTACC65550.013.9803056
CGTATTT79800.013.8674373
CGCGGAT50800.013.7434421
TGTCCGT61050.013.6921053
GTATTTA81000.013.2515484
GCGTGTA36850.012.7597144
Produced by FastQC (version 0.11.2)