##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l05n02 kx12-1.3420000000cbe0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17814800 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.409663313649325 33.0 33.0 33.0 33.0 33.0 2 32.380390798661786 33.0 33.0 33.0 33.0 33.0 3 32.37780968632822 33.0 33.0 33.0 33.0 33.0 4 36.208501751352806 37.0 37.0 37.0 37.0 37.0 5 36.1976616633361 37.0 37.0 37.0 37.0 37.0 6 36.17243359453937 37.0 37.0 37.0 37.0 37.0 7 36.23512349282619 37.0 37.0 37.0 37.0 37.0 8 36.213844724611 37.0 37.0 37.0 37.0 37.0 9 36.2111532545973 37.0 37.0 37.0 37.0 37.0 10-11 36.19556475514741 37.0 37.0 37.0 37.0 37.0 12-13 36.189901458338014 37.0 37.0 37.0 37.0 37.0 14-15 38.37863231694995 40.0 37.0 40.0 37.0 40.0 16-17 38.3323653647529 40.0 37.0 40.0 37.0 40.0 18-19 38.334537547432475 40.0 37.0 40.0 37.0 40.0 20-21 38.29859302377798 40.0 37.0 40.0 37.0 40.0 22-23 38.268891315086336 40.0 37.0 40.0 37.0 40.0 24-25 38.22808541212924 40.0 37.0 40.0 37.0 40.0 26-27 38.177453830522936 40.0 37.0 40.0 37.0 40.0 28-29 38.12697091743943 40.0 37.0 40.0 37.0 40.0 30-31 38.082486949053596 40.0 37.0 40.0 37.0 40.0 32-33 38.0035693917417 40.0 37.0 40.0 33.0 40.0 34-35 37.928812504209986 40.0 37.0 40.0 33.0 40.0 36-37 37.84439126456654 40.0 37.0 40.0 33.0 40.0 38-39 37.737723914947125 40.0 37.0 40.0 33.0 40.0 40-41 37.619802664077056 40.0 37.0 40.0 33.0 40.0 42-43 37.51374584615039 40.0 37.0 40.0 33.0 40.0 44-45 37.38416128724431 40.0 37.0 40.0 33.0 40.0 46-47 37.22980131688259 40.0 37.0 40.0 33.0 40.0 48-49 37.089125895322994 40.0 37.0 40.0 33.0 40.0 50-51 35.607127220064214 38.5 35.0 38.5 30.0 38.5 52-53 35.94215343422323 37.0 35.0 38.5 33.0 40.0 54-55 36.66185185912836 37.0 37.0 40.0 33.0 40.0 56-57 36.62745253946157 37.0 37.0 40.0 33.0 40.0 58-59 36.50334631879112 37.0 37.0 40.0 33.0 40.0 60-61 36.32558577699441 37.0 37.0 40.0 33.0 40.0 62-63 36.13541516042841 37.0 37.0 40.0 33.0 40.0 64-65 35.94754967779599 37.0 37.0 40.0 33.0 40.0 66-67 35.73372041785482 37.0 37.0 38.5 33.0 40.0 68-69 35.52709135101152 37.0 37.0 37.0 33.0 40.0 70-71 35.32953813683005 37.0 37.0 37.0 33.0 40.0 72-73 35.120980757572354 37.0 33.0 37.0 33.0 40.0 74-75 34.90658842647686 37.0 33.0 37.0 27.0 37.0 76-77 34.72141045647439 37.0 33.0 37.0 27.0 37.0 78-79 34.55291953881043 37.0 33.0 37.0 27.0 37.0 80-81 34.41228649774345 37.0 33.0 37.0 27.0 37.0 82-83 34.308470204549025 37.0 33.0 37.0 27.0 37.0 84-85 34.211215590407974 37.0 33.0 37.0 27.0 37.0 86-87 34.1233768832656 37.0 33.0 37.0 27.0 37.0 88-89 34.05205348923367 37.0 33.0 37.0 27.0 37.0 90-91 33.94958744976087 37.0 33.0 37.0 27.0 37.0 92-93 33.87492169432157 37.0 33.0 37.0 27.0 37.0 94-95 33.79942239037205 37.0 33.0 37.0 27.0 37.0 96-97 33.70880116532321 37.0 33.0 37.0 27.0 37.0 98-99 33.63779408132564 37.0 33.0 37.0 27.0 37.0 100-101 31.854525142016747 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11083650123310207 2306 2 0.2517139785013782 2306 3 0.27468641873150546 2306 4 0.37717171064988264 2306 5 0.4079172469289034 2306 6 0.3841915527627222 2306 7 0.38188910222974926 2306 8 0.403514750394919 2306 9 0.41448766703168616 2306 10-11 0.39814190420956663 2306 12-13 0.41215422771245613 2306 14-15 0.6799191125043293 2306 16-17 0.7028263070784035 2306 18-19 0.711541587649485 2306 20-21 0.7197056136003752 2306 22-23 0.6906583748594883 2306 24-25 0.6935607667429906 2306 26-27 0.7397869000291308 2306 28-29 0.6914930757741047 2306 30-31 0.7249542303672101 2306 32-33 0.7589998864821297 2306 34-35 0.7673926784817979 2306 36-37 0.720047118752511 2306 38-39 0.7628273088206257 2306 40-41 0.807261639013511 2306 42-43 0.7934541471925485 2306 44-45 0.8230842428995615 2306 46-47 0.8292586783310014 2306 48-49 0.8172094419904568 2306 50-51 0.7261770581877016 2306 52-53 0.7529352371158922 2306 54-55 0.800073515403966 2306 56-57 0.8219175554307157 2306 58-59 0.8451013553977802 2306 60-61 0.827097458527291 2306 62-63 0.8664238434497236 2306 64-65 0.9034128627082865 2306 66-67 0.855341018850126 2306 68-69 0.8675992733914768 2306 70-71 0.8459698784287113 2306 72-73 0.8338119801035973 2306 74-75 0.8188092152116155 2306 76-77 0.8775105187176635 2306 78-79 0.9138901291629722 2306 80-81 0.8079552841164599 2306 82-83 0.856961865273945 2306 84-85 0.8994515440457178 2306 86-87 0.9240308446106127 2306 88-89 0.8488266058029552 2306 90-91 0.9094141361469283 2306 92-93 0.9043756812993422 2306 94-95 0.8853885880558678 2306 96-97 0.9561159444331366 2306 98-99 0.8763226416985006 2306 100-101 0.5275254810856502 2307 1 0.028922773510153377 2307 2 0.16405629743982786 2307 3 0.20245158323001533 2307 4 0.261553187278011 2307 5 0.32245453177532823 2307 6 0.26431235088649174 2307 7 0.19428116507643267 2307 8 0.25416132545875314 2307 9 0.27117510571614645 2307 10-11 0.2667183603624821 2307 12-13 0.3024229533258662 2307 14-15 0.47088065714416416 2307 16-17 0.5107228014520047 2307 18-19 0.49441251327127844 2307 20-21 0.5225727180049233 2307 22-23 0.5026796455929414 2307 24-25 0.47100087246049327 2307 26-27 0.5383192845261391 2307 28-29 0.4651608464661763 2307 30-31 0.47940070385816114 2307 32-33 0.4647264251975898 2307 34-35 0.523782260090421 2307 36-37 0.5355486545797206 2307 38-39 0.5266917211393292 2307 40-41 0.584108083738947 2307 42-43 0.4958343017135576 2307 44-45 0.47080801229633806 2307 46-47 0.49347091503455687 2307 48-49 0.4483568199141672 2307 50-51 0.35608105116823197 2307 52-53 0.37846493633545464 2307 54-55 0.5132649381754817 2307 56-57 0.5194186367968996 2307 58-59 0.5708707742982995 2307 60-61 0.5547786562448493 2307 62-63 0.5057345520280805 2307 64-65 0.5606009000004732 2307 66-67 0.5545498928399581 2307 68-69 0.5441413717801566 2307 70-71 0.538693799950785 2307 72-73 0.5556616726509347 2307 74-75 0.5215722361263957 2307 76-77 0.5522874317571009 2307 78-79 0.5612756591324057 2307 80-81 0.5713181084811083 2307 82-83 0.5608986445537525 2307 84-85 0.5239998755290713 2307 86-87 0.5932396526440229 2307 88-89 0.6152326275817614 2307 90-91 0.570740619799416 2307 92-93 0.5211418464206119 2307 94-95 0.5578967065467992 2307 96-97 0.4742590137003617 2307 98-99 0.4365528232297251 2307 100-101 0.39888247915780894 2201 1 0.22726355447748148 2201 2 0.2916579236576524 2201 3 0.28771180133551155 2201 4 0.34169579143632944 2201 5 0.3601430633929752 2201 6 0.3798641200852586 2201 7 0.3621770026892577 2201 8 0.40675014004203547 2201 9 0.4157199744374296 2201 10-11 0.3771672250966773 2201 12-13 0.40553541223244594 2201 14-15 0.637397528821289 2201 16-17 0.6604858773600597 2201 18-19 0.6627611298388061 2201 20-21 0.65849991138235 2201 22-23 0.6314201132317194 2201 24-25 0.6746964022653046 2201 26-27 0.6510618607317937 2201 28-29 0.6764311499603863 2201 30-31 0.6367404360572735 2201 32-33 0.6346132267467439 2201 34-35 0.7149327224666706 2201 36-37 0.7126390391069393 2201 38-39 0.688633638795018 2201 40-41 0.7378991250846667 2201 42-43 0.7097170729572753 2201 44-45 0.6184497872481742 2201 46-47 0.7155540537992451 2201 48-49 0.7065298963400721 2201 50-51 0.6690502051114038 2201 52-53 0.7311019123765945 2201 54-55 0.6873351192316477 2201 56-57 0.6651060591114089 2201 58-59 0.6751083395884478 2201 60-61 0.7319193141907974 2201 62-63 0.7722455385863896 2201 64-65 0.7658676199757011 2201 66-67 0.7640073967801015 2201 68-69 0.7474423374460955 2201 70-71 0.7363341934442289 2201 72-73 0.7433878814913228 2201 74-75 0.7408488268207165 2201 76-77 0.7904908376910669 2201 78-79 0.7898429552606245 2201 80-81 0.8310894540483673 2201 82-83 0.7686370348874547 2201 84-85 0.7746356115455768 2201 86-87 0.8552135228225168 2201 88-89 0.8262356546726366 2201 90-91 0.8436302584822926 2201 92-93 0.7861655445306397 2201 94-95 0.815129827809919 2201 96-97 0.7983476217145125 2201 98-99 0.8427592027197406 2201 100-101 0.6449381171427397 1101 1 0.2199266570528451 1101 2 0.19922382227784396 1101 3 0.23619925076329196 1101 4 0.2872215365597768 1101 5 0.3152697059258216 1101 6 0.2818580233154293 1101 7 0.30630483502714156 1101 8 0.3300862602467163 1101 9 0.31948732854660733 1101 10-11 0.31712991307524874 1101 12-13 0.3413627210467709 1101 14-15 0.5563257432463828 1101 16-17 0.6133037957331098 1101 18-19 0.5664521703850909 1101 20-21 0.5832118803264308 1101 22-23 0.6051439759487565 1101 24-25 0.6233555864146325 1101 26-27 0.5926494042591699 1101 28-29 0.6291465211408465 1101 30-31 0.6123271321048165 1101 32-33 0.6153645301040385 1101 34-35 0.6054237781528613 1101 36-37 0.6455188267907843 1101 38-39 0.6186828513829497 1101 40-41 0.6367792690043146 1101 42-43 0.6181169541816587 1101 44-45 0.6292315933854766 1101 46-47 0.6912468645494059 1101 48-49 0.6681890002249276 1101 50-51 0.6581633266673563 1101 52-53 0.700763417558143 1101 54-55 0.6537158091810227 1101 56-57 0.6304326052573757 1101 58-59 0.682349606177084 1101 60-61 0.6965016405986688 1101 62-63 0.6449747884892147 1101 64-65 0.6667634070704409 1101 66-67 0.709540714250636 1101 68-69 0.7172815561485777 1101 70-71 0.7164622189488483 1101 72-73 0.7292534486308213 1101 74-75 0.7078577519939842 1101 76-77 0.7439132947540301 1101 78-79 0.7699749646345708 1101 80-81 0.7073088819999569 1101 82-83 0.7017008443845469 1101 84-85 0.7408650222948836 1101 86-87 0.7141751164411758 1101 88-89 0.737745994287252 1101 90-91 0.805611702325912 1101 92-93 0.7881906681165489 1101 94-95 0.7842491163955785 1101 96-97 0.8005195733502646 1101 98-99 0.7620725524693341 1101 100-101 0.6895970016571731 2304 1 0.0841173804460027 2304 2 0.16223906371507013 2304 3 0.21227339592115158 2304 4 0.2891425519631099 2304 5 0.3024645036279239 2304 6 0.31442200988061586 2304 7 0.30505302210242746 2304 8 0.32957210155885974 2304 9 0.31885234636377646 2304 10-11 0.3218708701261974 2304 12-13 0.29945353964593835 2304 14-15 0.5139011189893097 2304 16-17 0.5600183676127557 2304 18-19 0.5319097940064879 2304 20-21 0.571850021876152 2304 22-23 0.5451660652083135 2304 24-25 0.5742140691714681 2304 26-27 0.5646126444589896 2304 28-29 0.5865941305045013 2304 30-31 0.5907846379512094 2304 32-33 0.6151085395115743 2304 34-35 0.5816605771949881 2304 36-37 0.5939136715850211 2304 38-39 0.6230791153060906 2304 40-41 0.5357147648801828 2304 42-43 0.5735872596916849 2304 44-45 0.6269082619855837 2304 46-47 0.6240081233868437 2304 48-49 0.5634104857154014 2304 50-51 0.5566637714195366 2304 52-53 0.5923784668904801 2304 54-55 0.6216568469581318 2304 56-57 0.6191423029028371 2304 58-59 0.6393973282974272 2304 60-61 0.5989928144814414 2304 62-63 0.6080711279516038 2304 64-65 0.6222834015219476 2304 66-67 0.6380689772167045 2304 68-69 0.663236631762139 2304 70-71 0.6616679739349465 2304 72-73 0.6138664498039148 2304 74-75 0.6360120455847422 2304 76-77 0.6850365332783994 2304 78-79 0.7074599922082854 2304 80-81 0.6237403318241661 2304 82-83 0.7381185895637543 2304 84-85 0.6511804861869237 2304 86-87 0.5497619354420706 2304 88-89 0.6564423179331556 2304 90-91 0.7123785818455346 2304 92-93 0.7282050970325216 2304 94-95 0.663558069431069 2304 96-97 0.6403975052169599 2304 98-99 0.6187587130726371 2304 100-101 0.6677433590873072 2202 1 0.16063616621814703 2202 2 0.2204755077513596 2202 3 0.2423033061155735 2202 4 0.3041748711093746 2202 5 0.32041417655359083 2202 6 0.3633555209953627 2202 7 0.34766456782212884 2202 8 0.3906291421273522 2202 9 0.3751014504913357 2202 10-11 0.3608971842508524 2202 12-13 0.3844801893183032 2202 14-15 0.6008959240480678 2202 16-17 0.623687400249544 2202 18-19 0.6266216941318064 2202 20-21 0.6378836859953196 2202 22-23 0.6338886566526156 2202 24-25 0.6999909546283831 2202 26-27 0.6798824234777285 2202 28-29 0.6823591351052798 2202 30-31 0.6881005769144295 2202 32-33 0.6670499381745358 2202 34-35 0.6508402126672976 2202 36-37 0.6938437436382685 2202 38-39 0.7123198098148222 2202 40-41 0.7234468797584626 2202 42-43 0.7609553162040186 2202 44-45 0.768461429300622 2202 46-47 0.7547028692106679 2202 48-49 0.7008839421869197 2202 50-51 0.6467046092920583 2202 52-53 0.710707463546008 2202 54-55 0.7928335295139277 2202 56-57 0.8048658551457635 2202 58-59 0.7358079114282816 2202 60-61 0.76989433945743 2202 62-63 0.7946605843752153 2202 64-65 0.8322375981724903 2202 66-67 0.8211422227794287 2202 68-69 0.8921331007818623 2202 70-71 0.837109493136218 2202 72-73 0.8045797680839755 2202 74-75 0.8385932408095087 2202 76-77 0.8871328778043619 2202 78-79 0.8642685199099063 2202 80-81 0.8359863712666211 2202 82-83 0.9072129107637039 2202 84-85 0.892371597051806 2202 86-87 0.9362468023497499 2202 88-89 0.9574074772197321 2202 90-91 0.9008495185643142 2202 92-93 0.9271605143073742 2202 94-95 0.9230681371321126 2202 96-97 0.9865134559376543 2202 98-99 0.9828653790167579 2202 100-101 1.0320048741250822 1102 1 0.05665633347411614 1102 2 0.08392849053910112 1102 3 0.09475419120900597 1102 4 0.15801242122337555 1102 5 0.14860205382721858 1102 6 0.12706676993063581 1102 7 0.16492791535598883 1102 8 0.18195513936171892 1102 9 0.17703816062591216 1102 10-11 0.16053442498959924 1102 12-13 0.14693702108214524 1102 14-15 0.2463037687167784 1102 16-17 0.305743730640188 1102 18-19 0.24778879814660826 1102 20-21 0.2621138688746356 1102 22-23 0.28979833105218944 1102 24-25 0.2575552858180927 1102 26-27 0.3083945182097665 1102 28-29 0.3158265025388687 1102 30-31 0.3140021807223192 1102 32-33 0.32288321942318277 1102 34-35 0.3612016894346013 1102 36-37 0.33946843232174473 1102 38-39 0.33645792261339125 1102 40-41 0.3370183450977038 1102 42-43 0.28636559189178 1102 44-45 0.30349485890430117 1102 46-47 0.36312922028249517 1102 48-49 0.310288930433984 1102 50-51 0.20987264810014494 1102 52-53 0.2910093818921027 1102 54-55 0.3726166844686176 1102 56-57 0.3819732480148801 1102 58-59 0.3711882662076391 1102 60-61 0.3708307223473639 1102 62-63 0.3000282079932788 1102 64-65 0.39868246054243883 1102 66-67 0.46413840945964324 1102 68-69 0.39070239865996115 1102 70-71 0.3734953767578091 1102 72-73 0.40426634799826644 1102 74-75 0.41099447602275774 1102 76-77 0.43070135100123963 1102 78-79 0.42046839656583046 1102 80-81 0.41816024900326454 1102 82-83 0.37084128451757437 1102 84-85 0.3648684241803295 1102 86-87 0.41697808823053606 1102 88-89 0.42390660469617103 1102 90-91 0.43594541113656504 1102 92-93 0.4592175841029231 1102 94-95 0.3947100911005208 1102 96-97 0.39758100771236116 1102 98-99 0.44693874055602834 1102 100-101 0.524922171196696 2305 1 0.10289648031602638 2305 2 0.13351779266987762 2305 3 0.1051763033908415 2305 4 0.18462537884126817 2305 5 0.17038328244067458 2305 6 0.2008276709353325 2305 7 0.17549687760108412 2305 8 0.1728690892938829 2305 9 0.19098966278963303 2305 10-11 0.18398657739975732 2305 12-13 0.18288425387479634 2305 14-15 0.3205254083814779 2305 16-17 0.35197138901517633 2305 18-19 0.3132470782670893 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-1.336023052898618 1213 100-101 -1.1360320356079647 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17221.0 3 1251.0 4 714.0 5 620.0 6 632.0 7 1025.0 8 1787.0 9 3644.0 10 8570.0 11 13253.0 12 15767.0 13 18787.0 14 21849.0 15 24934.0 16 28752.0 17 33408.0 18 38549.0 19 43493.0 20 48568.0 21 55729.0 22 62320.0 23 70689.0 24 81044.0 25 93246.0 26 107964.0 27 130040.0 28 153225.0 29 183360.0 30 227022.0 31 289783.0 32 378387.0 33 505684.0 34 696880.0 35 1084916.0 36 2291815.0 37 5807927.0 38 5267497.0 39 4448.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.65409469585732 12.004400798663461 9.794339943430346 57.54716456204887 2 21.03787351435658 18.43710927689775 42.19558587314414 18.32943133560153 3 23.89474231366296 23.135505714265644 24.295728524967863 28.67402344710353 4 27.729396058791377 28.18912729802393 17.440523138529944 26.640953504654753 5 28.747185511585222 29.597070807588306 21.631418048043603 20.024325632782876 6 20.713574909220224 32.28987915061508 23.584916687609216 23.41162925255548 7 18.987154257372747 14.273867847474072 41.86363243491849 24.875345460234687 8 20.9127625506073 19.281982860322312 28.393277585013486 31.4119770040569 9 22.003828253889367 18.84422068194439 31.415430059289495 27.736521004876746 10-11 25.365089559333693 27.716177992643765 21.2466132198769 25.672119228145647 12-13 23.13390723720038 22.149573112334284 27.773570080994386 26.942949569470947 14-15 24.094398026235353 23.70660150410175 26.009279144746955 26.189721324915936 16-17 25.296781003519637 24.051905709320508 24.598908294199777 26.052404992960078 18-19 24.817174506904703 24.208051783989898 24.636637591078276 26.338136118027123 20-21 24.784121618059974 24.46243257204907 25.008167611390096 25.74527819850086 22-23 24.80368789385536 24.47617967719826 24.815690194699243 25.90444223424713 24-25 24.64477230859131 24.55072599158819 24.798195147105492 26.00630655271501 26-27 24.59081037906783 24.65588205881713 25.1013554932308 25.651952068884242 28-29 24.901102727457825 24.337290685601477 24.736881671612128 26.02472491532857 30-31 24.68670581461489 24.35110581706475 24.818621817435826 26.143566550884533 32-33 24.91997252122997 24.49621037508993 24.889953437127534 25.693863666552563 34-35 25.158102122584648 24.517347620656068 24.482030222116315 25.842520034642973 36-37 24.84537898314176 24.567084476548416 24.49182744178181 26.095709098528015 38-39 25.039128274980126 24.613548213690123 24.848143622886504 25.499179888443248 40-41 25.1950948292638 24.431971559964147 24.598760342172415 25.774173268599633 42-43 24.79500891866762 24.263261396047188 24.87248446863526 26.069245216649932 44-45 25.043227699723158 24.519706277602943 24.922695461375387 25.514370561298517 46-47 25.243024931814716 24.38391213814012 24.572232127233285 25.80083080281188 48-49 24.934857279300996 24.280254161701173 24.645114132161126 26.139774426836702 50-51 25.013614319737286 24.54356083365484 24.85206681438411 25.590758032223764 52-53 25.28196821019361 24.33118276560644 24.605591802031444 25.781257222168506 54-55 24.872842715204154 24.320087088557173 24.720853041656426 26.086217154582247 56-57 24.904841637158516 24.561165317683116 24.996932414753932 25.537060630404433 58-59 25.23076961117761 24.447665888632823 24.5146832545067 25.806881245682867 60-61 24.897622182102193 24.461692460013502 24.69721623375288 25.943469124131425 62-63 25.046735551901495 24.642418945634713 24.873441754943997 25.4374037475198 64-65 25.133413923837338 24.591278270985555 24.572590332831588 25.702717472345523 66-67 25.030666001734275 24.454903686679135 24.62740670887554 25.887023602711047 68-69 25.099298824949035 24.67278240024347 24.791893498615227 25.436025276192264 70-71 25.343687154128187 24.52058571270738 24.487204660579327 25.648522472585107 72-73 25.139917502703174 24.4458186719071 24.594943926358443 25.819319899031278 74-75 25.16704282079018 24.681616067304773 24.740383091616085 25.41095802028896 76-77 25.36576357820326 24.57529864128348 24.45688325657856 25.602054523934697 78-79 25.268795574002272 24.469197886314046 24.548088166044753 25.71391837363893 80-81 25.263268874675216 24.741800298956164 24.59181628224192 25.403114544126698 82-83 25.494359078351952 24.628262566456357 24.317276719232236 25.560101635959455 84-85 25.16059740827876 24.564905151294468 24.599599368213955 25.674898072212816 86-87 25.2912307359673 24.849091022212 24.634393168111444 25.225285073709248 88-89 25.443949927113245 24.573664747000674 24.27671079290539 25.705674532980694 90-91 25.35900307118458 24.43518278861739 24.407191896913698 25.79862224328433 92-93 25.356883993577096 24.727229454557257 24.57775875152034 25.338127800345312 94-95 25.583666009047146 24.56769063433229 24.342553053923854 25.50609030269671 96-97 25.274107322772117 24.583230616293527 24.49750046695098 25.645161593983374 98-99 25.45885692472549 24.742251905806867 24.52135688082018 25.277534288647463 100-101 25.809574756500233 24.57095030832641 24.14055686640082 25.478918068772533 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15461.0 1 8229.0 2 784.0 3 505.5 4 415.0 5 381.0 6 359.5 7 348.5 8 353.0 9 352.0 10 372.5 11 407.0 12 442.5 13 514.0 14 589.5 15 663.5 16 778.0 17 897.5 18 1066.0 19 1276.5 20 1651.0 21 2267.5 22 3049.5 23 4374.5 24 6369.5 25 9276.5 26 13645.0 27 19501.0 28 27315.0 29 37682.0 30 50312.5 31 66057.5 32 85539.5 33 110251.5 34 141572.0 35 180270.0 36 228387.5 37 287908.0 38 358364.0 39 433709.0 40 510498.5 41 582158.5 42 644546.0 43 687861.5 44 708979.0 45 711187.0 46 694039.5 47 665567.0 48 632109.5 49 597670.5 50 563604.0 51 535236.0 52 513736.0 53 501287.5 54 500175.0 55 497134.0 56 490997.0 57 485167.5 58 468869.5 59 455495.5 60 445657.0 61 426400.0 62 412853.5 63 391538.0 64 350734.0 65 319105.0 66 300375.5 67 281407.5 68 259759.0 69 227380.5 70 192651.5 71 159150.5 72 128937.5 73 106235.5 74 81319.0 75 58701.5 76 42711.0 77 30017.0 78 20029.0 79 13713.0 80 9080.0 81 5586.0 82 3444.5 83 2120.0 84 1343.5 85 967.5 86 722.0 87 546.5 88 455.0 89 376.0 90 312.5 91 253.0 92 196.0 93 155.5 94 96.5 95 68.0 96 54.0 97 34.5 98 14.5 99 5.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12672047960123042 2 0.11265352403619462 3 0.10903855221501223 4 0.12112400925073535 5 0.12375103846240204 6 0.11276017693154007 7 0.11463502256550734 8 0.11329344140826728 9 0.11081797157419673 10-11 0.0991310595684487 12-13 0.10872420683925724 14-15 0.1428306801086737 16-17 0.1533303769899185 18-19 0.14237319532074455 20-21 0.16622415070615443 22-23 0.14899970810786536 24-25 0.1352695511597099 26-27 0.14138244605608818 28-29 0.1304112311112109 30-31 0.10030424141724859 32-33 0.0998495632844601 34-35 0.12276309585288636 36-37 0.1222522846172845 38-39 0.13089116914026538 40-41 0.16688932797449313 42-43 0.13486539281945348 44-45 0.13541549722702473 46-47 0.15597985944271053 48-49 0.18242416417809912 50-51 0.15415553360127535 52-53 0.11336922109706536 54-55 0.11506724745717044 56-57 0.11147192222197273 58-59 0.11315591530637448 60-61 0.11461256932438198 62-63 0.10023126838359117 64-65 0.11170206794350764 66-67 0.13201102454139255 68-69 0.12443305566158475 70-71 0.11380425264386913 72-73 0.1199480207467948 74-75 0.16455699755259673 76-77 0.17522228708714102 78-79 0.1718037811258055 80-81 0.145409996182949 82-83 0.12726497069852033 84-85 0.13275759480881066 86-87 0.12417203673350248 88-89 0.12807328737903317 90-91 0.14080708175225093 92-93 0.11606922334238946 94-95 0.11546298583200484 96-97 0.11887026517277768 98-99 0.11691121988459034 100-101 0.10920414486831173 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.78148E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.97336638045215 #Duplication Level Percentage of deduplicated Percentage of total 1 76.8336692457683 40.701381113105626 2 11.876351214056008 12.582606082502332 3 4.283478865549409 6.8073088598301705 4 2.0836947184341454 4.415212949785002 5 1.2364499601379193 3.2749458374740734 6 0.7378221803124376 2.345095480878887 7 0.5689715584120919 2.10982371766744 8 0.4276623919875678 1.8123773262318374 9 0.312518288183568 1.4899631202485892 >10 1.4819079019506223 14.047540298632091 >50 0.09907587932814897 3.65262990054518 >100 0.05525987733436443 5.118465946525641 >500 0.002470611769910995 0.8640510093225914 >1k 6.117766472691819E-4 0.5561604527619297 >5k 4.486272703997723E-5 0.15519241449313098 >10k+ 1.0667401183537733E-5 0.06724548999551477 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.043862406538383816 4.490648225071289E-5 5.613310281339111E-6 2 0.0443339246020163 5.0519792532052004E-5 5.613310281339111E-6 3 0.04604598423782473 5.0519792532052004E-5 5.613310281339111E-6 4 0.05295596919415318 5.0519792532052004E-5 5.613310281339111E-6 5 0.05397759166535689 5.0519792532052004E-5 1.6839930844017334E-5 6 0.05704245907896805 6.174641309473022E-5 1.6839930844017334E-5 7 0.057637469968789996 6.735972337606933E-5 1.6839930844017334E-5 8 0.059461795810225204 7.297303365740845E-5 2.8066551406695557E-5 9 0.06177447964613692 7.858634393874755E-5 3.929317196937378E-5 10-11 0.06641107393852302 7.858634393874755E-5 3.929317196937378E-5 12-13 0.07066034982149674 7.858634393874755E-5 5.332644767272156E-5 14-15 0.07516503132227137 8.700630936075622E-5 5.8939757954060666E-5 16-17 0.07990266519972158 1.0384624020477356E-4 6.455306823539977E-5 18-19 0.0843259537014168 1.1226620562678223E-4 6.735972337606933E-5 20-21 0.08785111255809776 1.2910613647079954E-4 6.735972337606933E-5 22-23 0.09109841255585244 1.4875272245548645E-4 7.5779688798078E-5 24-25 0.09393874755821002 1.571726878774951E-4 8.981296450142578E-5 26-27 0.09636931091002987 1.6839930844017334E-4 1.0665289534544313E-4 28-29 0.09873812784875496 1.824325841435211E-4 1.1226620562678223E-4 30-31 0.10078698610144374 1.9085254956552978E-4 1.3471944675213867E-4 32-33 0.10250185239239284 2.02079170128208E-4 1.6839930844017334E-4 34-35 0.10411567909827785 2.1330579069088622E-4 1.76819273862182E-4 36-37 0.10549936008262792 2.1891910097222533E-4 1.824325841435211E-4 38-39 0.10695320744549476 2.1891910097222533E-4 1.9365920470619936E-4 40-41 0.10840424815322092 2.3014572153490356E-4 2.1330579069088622E-4 42-43 0.10981318903383704 2.582122729415991E-4 2.2453241125356446E-4 44-45 0.11121090329389047 2.890854794889643E-4 2.3575903181624266E-4 46-47 0.11266194400161664 2.975054449109729E-4 2.3575903181624266E-4 48-49 0.11489323483844893 3.087320654736511E-4 2.3575903181624266E-4 50-51 0.11729853829400275 3.227653411769989E-4 2.4979230751959045E-4 52-53 0.1197908480589173 3.367986168803467E-4 2.5540561780092955E-4 54-55 0.12123066214608078 3.424119271616858E-4 2.610189280822687E-4 56-57 0.12269854278465096 3.480252374430249E-4 2.722455486449469E-4 58-59 0.12404293059703168 3.5083189258369447E-4 2.7505220378561644E-4 60-61 0.12704605159754812 3.620585131463727E-4 2.7505220378561644E-4 62-63 0.1289265105417967 3.7889844399039004E-4 2.834721692076251E-4 64-65 0.13139917372072657 3.845117542717291E-4 2.975054449109729E-4 66-67 0.133391898870602 3.929317196937378E-4 3.087320654736511E-4 68-69 0.13556424994948021 4.069649953970856E-4 3.115387206143207E-4 70-71 0.13801165323214407 4.1257830567842466E-4 3.227653411769989E-4 72-73 0.14126175988503942 4.181916159597638E-4 3.3960527202101627E-4 74-75 0.14490760491276916 4.2941823652244203E-4 3.452185823023553E-4 76-77 0.14896602824617733 4.3222489166311155E-4 3.6486516828704226E-4 78-79 0.153810315018973 4.3222489166311155E-4 3.7047847856838136E-4 80-81 0.15986427015739724 4.434515122257898E-4 3.929317196937378E-4 82-83 0.1682645889934212 4.54678132788468E-4 4.0977165053775514E-4 84-85 0.17923861059343915 4.687114084918158E-4 4.1257830567842466E-4 86-87 0.1924102431686014 4.8555133933583317E-4 4.2099827110043335E-4 88-89 0.20956171273323304 4.995846150391809E-4 4.2380492624110287E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGTAT 4680 0.0 14.61392 1 CGCGAAT 2565 0.0 14.442998 1 CGAATTA 6325 0.0 14.158429 14-15 CGCGTAT 1210 0.0 13.738293 1 CTCGGGT 10365 0.0 13.655168 1 CTCGGAT 9005 0.0 12.974825 1 CTCGGTT 7765 0.0 12.96715 1 CTCGATT 9200 0.0 11.873811 1 CTCGTTT 7535 0.0 11.661075 1 TCGGGTC 9840 0.0 11.485539 8 CTCGACT 9180 0.0 11.382301 1 CTCGAAT 8240 0.0 11.182133 1 TTAGGTG 8390 0.0 10.956712 18-19 CCCGGTT 6360 0.0 10.902996 1 CTCGCAT 6890 0.0 10.891507 1 AATTAGG 8615 0.0 10.698185 16-17 CGCGCAT 2840 0.0 10.535916 1 CCCGTAT 2750 0.0 10.362598 1 GAGTGTC 8830 0.0 10.329002 24-25 CTCGTCT 15595 0.0 10.233025 1 >>END_MODULE