FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n02 kx12-1.3420000000cbe0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n02 kx12-1.3420000000cbe0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17814800
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT46800.014.613921
CGCGAAT25650.014.4429981
CGAATTA63250.014.15842914-15
CGCGTAT12100.013.7382931
CTCGGGT103650.013.6551681
CTCGGAT90050.012.9748251
CTCGGTT77650.012.967151
CTCGATT92000.011.8738111
CTCGTTT75350.011.6610751
TCGGGTC98400.011.4855398
CTCGACT91800.011.3823011
CTCGAAT82400.011.1821331
TTAGGTG83900.010.95671218-19
CCCGGTT63600.010.9029961
CTCGCAT68900.010.8915071
AATTAGG86150.010.69818516-17
CGCGCAT28400.010.5359161
CCCGTAT27500.010.3625981
GAGTGTC88300.010.32900224-25
CTCGTCT155950.010.2330251
Produced by FastQC (version 0.11.2)