FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n02 kx0-1.3420000000c9d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n02 kx0-1.3420000000c9d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18402123
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT12800.015.9548681
CCGCGTT44750.012.523441
TTAAAAA164150.012.46843756-57
CTCGGTT83850.012.2344771
TCGGGGG88200.011.90455738-39
CCGGTAT32100.011.6884461
CGGAAAT58600.011.6707731
CCGGAAT63350.011.3954551
CGAATTA50650.011.20846414-15
CGCGAAT22900.011.1993541
CTCCCGT85700.011.0836781
CGGGTAT24550.011.0270181
CGGGGAT73150.010.9725381
CGGGAAT67650.010.7413371
CTCGTAT46050.010.7260141
TTTAAAA128750.010.60391556-57
CTCGGGT85100.010.5479221
TAAAAAA193700.010.5047958-59
CGCGCAT24900.010.4905441
CGACAAT66900.010.2228291
Produced by FastQC (version 0.11.2)