FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n02 k12-1.3420000000cad4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n02 k12-1.3420000000cad4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21857922
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC399090.1825836875069826Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT139800.029.321679
ATCGGAA192000.022.1640973
AAGAGCG190100.021.6827938
GCCGTAT100000.020.52540448-49
GAGTGTA105500.019.1992628-29
CCGTATC112850.019.1144748-49
CGTGTAG111150.018.65709116-17
TCGGAAG224150.018.6460654
CGCCGTA116200.018.32004746-47
GATCGGA235000.018.3107282
GTAGGGA120350.018.03996818-19
CGTATCA113600.017.99981150-51
AGATCGG226550.017.987611
TCGTGTA119500.017.90987614-15
TGTAGGG117000.017.66318718-19
GTCGTGT119200.017.45675514-15
AGTGTAG118950.017.26790228-29
CGGAAGA245150.016.9508915
GTGTAGA128100.016.01480730-31
GTGTAGG138950.015.79619916-17
Produced by FastQC (version 0.11.2)