FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n01 mx48-2.3410000000cd91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n01 mx48-2.3410000000cd91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15936244
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT258780.16238456188296316TruSeq Adapter, Index 25 (97% over 44bp)
CTGACATGCGTGCGAGTCGACGGGTGCGGAAACCCGGAAGGCACAAGGAA190340.11943843229307986No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA166780.10465452210696573TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG62200.031.9870014
CATGCGT63550.030.8591795
TATGCCG74350.028.72298848-49
CGTATGC78100.027.46378346-47
CGCGTGT47250.027.3359853
CACACGT80900.026.70875512-13
CTCGTAT84150.025.4048644-45
CGCGTAT68350.024.7594641
GCGTGTA56600.022.9870914
CACGTCT96400.022.88235914-15
GCGTATT77100.021.3116912
TCACACT104050.020.9589730-31
AGCACAC109400.020.40113310-11
TCCGTAC72400.020.3316695
GAGCACA135700.020.1562759
CTGACAT118750.020.0154881
TAAGTCG82800.019.6138749
ATGCGTG116650.019.4170636
CGTCTGA114650.019.21844916-17
AGTCACA114250.019.21222328-29
Produced by FastQC (version 0.11.2)