FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n01 mx12-1.3410000000cc42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n01 mx12-1.3410000000cc42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19582760
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA238700.12189293031217255TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTA99650.018.88036236-37
TATGCCG99300.018.85030248-49
CGTATGC102950.018.45770846-47
CACACGT103050.018.43341812-13
CCGTATC103050.018.09652538-39
CTCGTAT110850.017.01390544-45
CGTCTGA116050.016.40903916-17
AGTTCCG129850.014.81814534-35
CGAATTA60250.013.67549114-15
AGTCACA144300.013.51577428-29
GTATCTC141500.013.48129240-41
AGCACAC153600.013.01566810-11
AGAGCAC193350.012.9921489
TCCGTAT97350.012.51857738-39
CGTATCT98600.012.504440-41
ATCGGAA194550.012.4237183
CACGTCT155900.012.32176614-15
TCGTATG104000.012.10477546-47
CGCGTAT19100.011.9476721
TCACAGT162850.011.87417830-31
Produced by FastQC (version 0.11.2)