FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n01 mx0-1.3410000000ca37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n01 mx0-1.3410000000ca37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26242500
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT1476820.5627588834905212TruSeq Adapter, Index 27 (97% over 44bp)
CTGACATGCGTGCGAGTCGACGGGTGCGGAAACCCGGAAGGCACAAGGAA277910.10590073354291701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA333150.045.8664479
AGAGCAC379400.040.049228
TCGGAAG379500.039.4770553
CGCGTAT47550.037.7913741
TATGCCG211100.037.4382248-49
CGTATGC213500.037.20392646-47
CTCGTAT218700.036.1896644-45
ATCGGAA436850.034.2651942
ACGTCTG220000.034.01787614-15
TCGTATG220350.033.81658644-45
TATCTCG222200.033.19829640-41
CACACGT242250.033.0102412-13
ACATGCG81250.032.960764
CACGTCT247250.032.2469114-15
CGGAAGA473850.032.0062984
GATCGGA469750.031.9187831
GTATGCC235550.031.76492746-47
ACACGTC241150.031.19164512-13
GCGTATT57200.031.0509512
CATGCGT87450.030.7868085
Produced by FastQC (version 0.11.2)