##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l05n01 m48-2.3410000000cc92.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22381281 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75998590071766 33.0 33.0 33.0 33.0 33.0 2 32.76310185283854 33.0 33.0 33.0 33.0 33.0 3 32.61853068195695 33.0 33.0 33.0 33.0 33.0 4 36.62195702739267 37.0 37.0 37.0 37.0 37.0 5 36.663139656751554 37.0 37.0 37.0 37.0 37.0 6 36.60159340298708 37.0 37.0 37.0 37.0 37.0 7 36.628635554863905 37.0 37.0 37.0 37.0 37.0 8 36.62491257761341 37.0 37.0 37.0 37.0 37.0 9 36.60812269860693 37.0 37.0 37.0 37.0 37.0 10-11 36.58845034383867 37.0 37.0 37.0 37.0 37.0 12-13 36.59942467100073 37.0 37.0 37.0 37.0 37.0 14-15 38.91163378896856 40.0 40.0 40.0 37.0 40.0 16-17 38.90207973350587 40.0 40.0 40.0 37.0 40.0 18-19 38.869284358656685 40.0 40.0 40.0 37.0 40.0 20-21 38.85635777505318 40.0 40.0 40.0 37.0 40.0 22-23 38.825494729278454 40.0 40.0 40.0 37.0 40.0 24-25 38.79313809160432 40.0 40.0 40.0 37.0 40.0 26-27 38.72554692021426 40.0 38.5 40.0 37.0 40.0 28-29 38.67385436964042 40.0 37.0 40.0 37.0 40.0 30-31 38.59924630766219 40.0 37.0 40.0 37.0 40.0 32-33 38.509109063060336 40.0 37.0 40.0 37.0 40.0 34-35 38.435951968075464 40.0 37.0 40.0 37.0 40.0 36-37 38.35201356437105 40.0 37.0 40.0 37.0 40.0 38-39 38.24218531101951 40.0 37.0 40.0 37.0 40.0 40-41 38.13047812142656 40.0 37.0 40.0 37.0 40.0 42-43 38.01198427829041 40.0 37.0 40.0 35.0 40.0 44-45 37.9000567706558 40.0 37.0 40.0 33.0 40.0 46-47 37.784549150694275 40.0 37.0 40.0 33.0 40.0 48-49 37.63217999005508 40.0 37.0 40.0 33.0 40.0 50-51 37.507294600340344 40.0 37.0 40.0 33.0 40.0 52-53 37.32813485966241 38.5 37.0 40.0 33.0 40.0 54-55 37.14426975828595 37.0 37.0 40.0 33.0 40.0 56-57 36.97322403038503 37.0 37.0 40.0 33.0 40.0 58-59 36.81399114286622 37.0 37.0 40.0 33.0 40.0 60-61 36.60858746199558 37.0 37.0 40.0 33.0 40.0 62-63 36.388685236559965 37.0 37.0 40.0 33.0 40.0 64-65 36.20827243981253 37.0 37.0 40.0 33.0 40.0 66-67 35.954814829410346 37.0 37.0 37.0 33.0 40.0 68-69 35.761863228472045 37.0 37.0 37.0 33.0 40.0 70-71 35.54503348132754 37.0 37.0 37.0 33.0 40.0 72-73 35.24401744475662 37.0 33.0 37.0 33.0 40.0 74-75 34.97377442336746 37.0 33.0 37.0 33.0 37.0 76-77 32.213316699790326 33.0 30.0 35.0 24.5 37.0 78-79 34.43430407312253 37.0 33.0 37.0 27.0 37.0 80-81 34.6844925230151 37.0 33.0 37.0 33.0 37.0 82-83 34.64719771848627 37.0 33.0 37.0 33.0 37.0 84-85 34.548778374213704 37.0 33.0 37.0 30.0 37.0 86-87 34.466670763840554 37.0 33.0 37.0 27.0 37.0 88-89 34.39163593897955 37.0 33.0 37.0 27.0 37.0 90-91 34.30596590963672 37.0 33.0 37.0 27.0 37.0 92-93 34.23417964771542 37.0 33.0 37.0 27.0 37.0 94-95 34.1574178216162 37.0 33.0 37.0 27.0 37.0 96-97 34.09100372762399 37.0 33.0 37.0 27.0 37.0 98-99 34.00505102902734 37.0 33.0 37.0 27.0 37.0 100-101 32.14293650126639 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.038347150194113055 2306 2 0.0509830590318785 2306 3 0.2094980995572584 2306 4 0.18889534953247988 2306 5 0.1612417314193877 2306 6 0.18100332390504548 2306 7 0.1582536960545582 2306 8 0.18988051219698576 2306 9 0.2044415275394229 2306 10-11 0.1903218777135507 2306 12-13 0.18893825580688883 2306 14-15 0.3699790746015452 2306 16-17 0.37086318151345665 2306 18-19 0.3299168635700056 2306 20-21 0.35069820745101055 2306 22-23 0.38124016172248787 2306 24-25 0.40003936636605886 2306 26-27 0.4163497753522378 2306 28-29 0.4496208244468036 2306 30-31 0.4383218146632686 2306 32-33 0.46475661391843914 2306 34-35 0.447365331787168 2306 36-37 0.4378574722701387 2306 38-39 0.43537187208855244 2306 40-41 0.47205986770953245 2306 42-43 0.4772452493814754 2306 44-45 0.4266633597347891 2306 46-47 0.42988751164629946 2306 48-49 0.4255049218274891 2306 50-51 0.36346203771449126 2306 52-53 0.32650394911755143 2306 54-55 0.38819124813323214 2306 56-57 0.3946593576365629 2306 58-59 0.382527215677932 2306 60-61 0.4172673293851261 2306 62-63 0.4429669480935985 2306 64-65 0.39749666588473076 2306 66-67 0.3633890901486865 2306 68-69 0.3589066535287273 2306 70-71 0.38482601024869467 2306 72-73 0.3883754529780745 2306 74-75 0.4005173086132672 2306 76-77 0.17025256896585006 2306 78-79 0.29450120488708365 2306 80-81 0.3585323846180728 2306 82-83 0.338965412741544 2306 84-85 0.3864401712059191 2306 86-87 0.41289465876013054 2306 88-89 0.3605051410627169 2306 90-91 0.40017901285708746 2306 92-93 0.3978708178855115 2306 94-95 0.33393801907570264 2306 96-97 0.32216785332540354 2306 98-99 0.3363230259285146 2306 100-101 0.13349671145272168 2307 1 1.1454044351211223E-4 2307 2 0.02747196890850745 2307 3 0.18089691437038624 2307 4 0.15743341490100704 2307 5 0.11085971373700687 2307 6 0.14183297093177316 2307 7 0.12247437442147913 2307 8 0.13278426964615875 2307 9 0.021556555173489755 2307 10-11 0.14953046991477947 2307 12-13 0.1399887030604745 2307 14-15 0.29986579235863076 2307 16-17 0.21378229503346802 2307 18-19 0.33225385760602677 2307 20-21 0.3176665971836101 2307 22-23 0.2964936532771745 2307 24-25 0.28910225870350814 2307 26-27 0.32967111298316354 2307 28-29 0.34287985932535037 2307 30-31 0.33592427249578805 2307 32-33 0.3836011770187895 2307 34-35 0.3857494487198778 2307 36-37 0.34643817394241694 2307 38-39 0.3383609432736776 2307 40-41 0.36534841418441744 2307 42-43 0.3237804470154728 2307 44-45 0.3127986713042574 2307 46-47 0.37726611966402857 2307 48-49 0.33281802959693607 2307 50-51 0.34323961156208327 2307 52-53 0.3584895860574946 2307 54-55 0.34935278778094414 2307 56-57 0.2918230902418415 2307 58-59 0.28390231366212504 2307 60-61 0.2995480021554897 2307 62-63 0.22271535163378076 2307 64-65 0.2894657879162139 2307 66-67 0.24685479096865492 2307 68-69 0.12853880913623783 2307 70-71 0.12049039571507336 2307 72-73 0.1951917010304598 2307 74-75 0.22264396140747778 2307 76-77 0.07707999410994404 2307 78-79 0.2533907884863993 2307 80-81 0.19282177830047686 2307 82-83 0.2736122373044054 2307 84-85 0.26592927674199274 2307 86-87 0.21575176698495824 2307 88-89 0.2828729378967978 2307 90-91 0.1803456136023769 2307 92-93 0.17714992984071642 2307 94-95 0.16029544051398403 2307 96-97 0.17433747772646058 2307 98-99 0.11076771022457876 2307 100-101 0.007328221675635405 2201 1 0.012252723073302718 2201 2 0.04368666628470663 2201 3 0.18305821041802517 2201 4 0.15224540213230142 2201 5 0.1242826606454912 2201 6 0.13905349279436763 2201 7 0.11581884491545225 2201 8 0.12199790633879815 2201 9 0.15306700134095053 2201 10-11 0.1635576975123243 2201 12-13 0.14566897286594127 2201 14-15 0.30608105932466856 2201 16-17 0.23544168610466443 2201 18-19 0.33265759137522366 2201 20-21 0.3166531796418752 2201 22-23 0.25350554109675016 2201 24-25 0.25797989428043167 2201 26-27 0.27148767125232354 2201 28-29 0.30753785379032195 2201 30-31 0.2871173004788403 2201 32-33 0.32565195175120465 2201 34-35 0.35013527564703395 2201 36-37 0.2951106394647667 2201 38-39 0.3144556384791173 2201 40-41 0.33544392190333383 2201 42-43 0.31323484986977945 2201 44-45 0.2560222472310656 2201 46-47 0.33170931434692363 2201 48-49 0.34318516834647994 2201 50-51 0.30451162080437655 2201 52-53 0.27115589075307867 2201 54-55 0.31267092823668463 2201 56-57 0.2902112253361864 2201 58-59 0.3277572145174048 2201 60-61 0.33280476416771876 2201 62-63 0.3865726989350833 2201 64-65 0.3176236306019433 2201 66-67 0.2571299749600442 2201 68-69 0.28519118743532346 2201 70-71 0.3488099019165958 2201 72-73 0.39316640019713134 2201 74-75 0.4018931062374165 2201 76-77 0.07968716045911606 2201 78-79 0.3964331253192128 2201 80-81 0.42332452287473643 2201 82-83 0.38944390848467947 2201 84-85 0.37717323715147444 2201 86-87 0.4143027351841013 2201 88-89 0.3960363776507023 2201 90-91 0.47052492228972653 2201 92-93 0.42109234644618 2201 94-95 0.46124889030362226 2201 96-97 0.3731532387870473 2201 98-99 0.3865002910677404 2201 100-101 0.2596155430267544 1101 1 0.015915362251831766 1101 2 0.016948883033926165 1101 3 0.17808556106425044 1101 4 0.13770811282801532 1101 5 0.12148416356337322 1101 6 0.09477175551683814 1101 7 0.09496536910953068 1101 8 0.08914602073382127 1101 9 0.14085751014939518 1101 10-11 0.14776598331396684 1101 12-13 0.1480214079955431 1101 14-15 0.2330360989391238 1101 16-17 0.21625323143578612 1101 18-19 0.2674772452494736 1101 20-21 0.28311571713874173 1101 22-23 0.2832000524776035 1101 24-25 0.21970055611265593 1101 26-27 0.26679709682498043 1101 28-29 0.2905053027966886 1101 30-31 0.25370022412631954 1101 32-33 0.30651426375527535 1101 34-35 0.29869313358715033 1101 36-37 0.2969571489635996 1101 38-39 0.3185617778422767 1101 40-41 0.26895910135500856 1101 42-43 0.3149937566944132 1101 44-45 0.28899715039850093 1101 46-47 0.2603527095852698 1101 48-49 0.2935369019017813 1101 50-51 0.2993325524380026 1101 52-53 0.33450538032769117 1101 54-55 0.300611779504365 1101 56-57 0.29902981485516733 1101 58-59 0.3153104501623858 1101 60-61 0.3225696224102066 1101 62-63 0.3522310784789937 1101 64-65 0.31268766102110135 1101 66-67 0.2932993217541764 1101 68-69 0.33947172502997347 1101 70-71 0.3164650675391627 1101 72-73 0.3644153647798589 1101 74-75 0.32983838678485 1101 76-77 -0.04501619397133538 1101 78-79 0.2989624666153148 1101 80-81 0.2409764253960418 1101 82-83 0.2620610013047937 1101 84-85 0.3214892027349876 1101 86-87 0.28159767520725865 1101 88-89 0.31152191559945663 1101 90-91 0.34349883681426974 1101 92-93 0.3626529904302842 1101 94-95 0.32772198092898464 1101 96-97 0.3100205847007942 1101 98-99 0.34390270442550985 1101 100-101 0.24305009963356383 2304 1 0.06050093217159258 2304 2 0.07415992872833499 2304 3 0.1897603915429258 2304 4 0.14928817730785937 2304 5 0.10813538137968948 2304 6 0.14181368703612662 2304 7 0.10912743039018835 2304 8 0.12593082756409757 2304 9 0.05776821987755909 2304 10-11 0.13081228445471282 2304 12-13 0.12658468103079912 2304 14-15 0.2416750432166026 2304 16-17 0.24606509538480736 2304 18-19 0.24110847818293735 2304 20-21 0.2562565221879183 2304 22-23 0.2922830963085019 2304 24-25 0.2292517221135597 2304 26-27 0.21555777017583466 2304 28-29 0.23099059118651155 2304 30-31 0.2675091746673459 2304 32-33 0.31144550497191403 2304 34-35 0.27698442827583847 2304 36-37 0.3382984182631503 2304 38-39 0.3298720013437091 2304 40-41 0.3151987734869408 2304 42-43 0.30736967686645045 2304 44-45 0.2527097336665136 2304 46-47 0.2689638155184184 2304 48-49 0.2344187912175073 2304 50-51 0.32692632228311425 2304 52-53 0.30232178755056793 2304 54-55 0.23852599619817738 2304 56-57 0.24077044613981258 2304 58-59 0.28969023497636925 2304 60-61 0.26746722005044177 2304 62-63 0.31056562649149555 2304 64-65 0.2937692302782011 2304 66-67 0.3223635553437063 2304 68-69 0.36133972033140793 2304 70-71 0.35855907235461615 2304 72-73 0.3775582308125678 2304 74-75 0.43617786980524187 2304 76-77 0.25891351857156053 2304 78-79 0.4435463791987857 2304 80-81 0.3952254186956381 2304 82-83 0.32109518349798094 2304 84-85 0.3783890721523022 2304 86-87 0.4163857211664279 2304 88-89 0.4102367897056638 2304 90-91 0.4648612674601722 2304 92-93 0.48791321675584953 2304 94-95 0.46696753621932885 2304 96-97 0.3926954042473554 2304 98-99 0.3651088689316637 2304 100-101 0.3748006791406482 2202 1 0.08211888729564265 2202 2 0.09345837082168629 2202 3 0.19726397427483278 2202 4 0.16435811751909313 2202 5 0.14225462382518117 2202 6 0.175140562494569 2202 7 0.12952674624077076 2202 8 0.1492141938098186 2202 9 0.15594353369124292 2202 10-11 0.1554096643540035 2202 12-13 0.15350613025299253 2202 14-15 0.3433149068250856 2202 16-17 0.31475700641235704 2202 18-19 0.32317905177133355 2202 20-21 0.35286439642164424 2202 22-23 0.3369759316553669 2202 24-25 0.34152722151171133 2202 26-27 0.39220460708820326 2202 28-29 0.3835204592957382 2202 30-31 0.40830011246487175 2202 32-33 0.45155453021362746 2202 34-35 0.41809144664722453 2202 36-37 0.43212936751221065 2202 38-39 0.4333282192038368 2202 40-41 0.48655928308907903 2202 42-43 0.4716032747399552 2202 44-45 0.4572132269239475 2202 46-47 0.5293914757099998 2202 48-49 0.4820533624711629 2202 50-51 0.543479500743544 2202 52-53 0.5362559184276989 2202 54-55 0.5188322488422514 2202 56-57 0.49446977258921976 2202 58-59 0.49387105342155735 2202 60-61 0.48622113268192635 2202 62-63 0.5435522475061703 2202 64-65 0.5293449887319639 2202 66-67 0.5716807082600823 2202 68-69 0.5791695726174666 2202 70-71 0.581044441927709 2202 72-73 0.5877897602645277 2202 74-75 0.6077768378763722 2202 76-77 0.723056504778981 2202 78-79 0.6573045876556805 2202 80-81 0.624509935949348 2202 82-83 0.6227604369884574 2202 84-85 0.6009276985510539 2202 86-87 0.6308335023046681 2202 88-89 0.6453977266534849 2202 90-91 0.6462299356026691 2202 92-93 0.6624259524557701 2202 94-95 0.6842512977440478 2202 96-97 0.6610907584506549 2202 98-99 0.6523912140274817 2202 100-101 0.7812210240437096 1102 1 0.03611910586575817 1102 2 0.031097213218451714 1102 3 0.16506772812910953 1102 4 0.09081747396479756 1102 5 0.09150454075619763 1102 6 0.08384631489801819 1102 7 0.07002691916406434 1102 8 0.01676042354736751 1102 9 0.07266094474369567 1102 10-11 0.07211941619981843 1102 12-13 0.08061664777272881 1102 14-15 0.17644235828525723 1102 16-17 0.16373981236733215 1102 18-19 0.09190750832502914 1102 20-21 0.08906746656064968 1102 22-23 0.1216960663011335 1102 24-25 0.1513994196813968 1102 26-27 0.14426641153971786 1102 28-29 0.1733673918503129 1102 30-31 0.16690005215761516 1102 32-33 0.15721454769511922 1102 34-35 0.15411758397259234 1102 36-37 0.14766450548485466 1102 38-39 0.1751603307309253 1102 40-41 0.10866000350685567 1102 42-43 0.028178223006896985 1102 44-45 0.12935135151425925 1102 46-47 0.1576487075431885 1102 48-49 0.12208379727969287 1102 50-51 0.131149799861781 1102 52-53 0.09651552338123537 1102 54-55 0.09171677082962759 1102 56-57 0.15929618206829588 1102 58-59 0.139700047394534 1102 60-61 0.17100510861413198 1102 62-63 0.19780473669692356 1102 64-65 0.1658007330135689 1102 66-67 0.12575645483224918 1102 68-69 0.013227311829396626 1102 70-71 0.13597343182949118 1102 72-73 0.11727666487173849 1102 74-75 0.1314048477903711 1102 76-77 -0.07917075138683316 1102 78-79 0.10883308000716596 1102 80-81 0.1267307203425716 1102 82-83 0.16893333447968928 1102 84-85 0.16134276226743083 1102 86-87 0.12968251374268647 1102 88-89 0.08107279216283558 1102 90-91 0.14151825127219553 1102 92-93 0.07238606700871486 1102 94-95 0.09181594401824356 1102 96-97 0.10093099305472464 1102 98-99 0.1413405595302848 1102 100-101 0.22493545499652612 2305 1 0.01953522327796975 2305 2 0.027035588568587343 2305 3 0.15116387508209783 2305 4 0.1151681119033725 2305 5 0.08692277561770823 2305 6 0.08027303230312555 2305 7 0.06202218252037284 2305 8 0.04040659050835416 2305 9 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-0.7208863445149305 1213 68-69 -0.7498086164140361 1213 70-71 -0.7829243205521905 1213 72-73 -0.689038098431439 1213 74-75 -0.7125141547465361 1213 76-77 -0.6749513080347853 1213 78-79 -0.700154237594937 1213 80-81 -0.6529716757294537 1213 82-83 -0.5975799946738363 1213 84-85 -0.5742938976121295 1213 86-87 -0.5726722439460659 1213 88-89 -0.638958232345388 1213 90-91 -0.6992066945904654 1213 92-93 -0.6503688514715336 1213 94-95 -0.5735661492832165 1213 96-97 -0.5454517526083009 1213 98-99 -0.6822596301435553 1213 100-101 -0.2223399196685527 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 58.0 8 693.0 9 2296.0 10 2485.0 11 1613.0 12 1028.0 13 870.0 14 1183.0 15 2299.0 16 4688.0 17 7893.0 18 12010.0 19 17399.0 20 24737.0 21 33416.0 22 44441.0 23 57771.0 24 75018.0 25 95920.0 26 121408.0 27 154227.0 28 195705.0 29 253305.0 30 327890.0 31 384193.0 32 478610.0 33 643739.0 34 903326.0 35 1416242.0 36 2987091.0 37 7635627.0 38 6491084.0 39 3013.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.54805715086961 12.743872834864812 15.004252390026574 49.70381762423901 2 23.495027569454034 17.682356152653053 36.45063113465986 22.37198514323305 3 25.377477764726823 22.389553728898743 25.82576667994003 26.407201826434402 4 27.772614981242583 27.07303929565068 18.74197459921977 26.41237112388697 5 29.419047842448652 28.947265068679062 21.517156640986716 20.116530447885562 6 22.227119171597014 32.704035126497004 22.27832267509621 22.79052302680977 7 19.897551887222185 16.177318000698886 40.90744403772063 23.0176860743583 8 21.74159386773232 20.861349306205902 27.39309816060565 30.003958665456132 9 22.75201316671731 20.46120595152708 30.18684676717119 26.599934114584418 10-11 25.35753203759874 28.82928595552685 20.998366894191626 24.81481511268278 12-13 23.352890301497936 23.01959838670539 27.628378375661338 25.999132936135332 14-15 23.85563409780404 24.997233146533677 26.046374385366455 25.10075837029583 16-17 24.970163643376537 24.727317448982294 25.134981015563206 25.167537892077963 18-19 24.84840468085329 24.96114696248845 25.523531360096918 24.666916996561344 20-21 24.707807287409235 24.88251191122194 25.700349914135543 24.709330887233282 22-23 24.56595312842013 25.370922692047877 25.158492938808998 24.904631240722995 24-25 24.631440890268973 25.05620210031767 25.144827501160457 25.1675295082529 26-27 24.45122555764346 24.889037852659104 25.21635379136699 25.443382798330443 28-29 24.74397913148939 25.176778308623177 24.751820505716363 25.327422054171073 30-31 24.821525221474154 24.711369066153217 25.248902594271076 25.218203118101556 32-33 24.661774127845735 25.225783781205646 24.782868017360457 25.329574073588162 34-35 24.80621047231331 25.098298226068444 24.62386541923607 25.471625882382178 36-37 24.79225610801938 24.993476175497975 24.99773115943284 25.2165365570498 38-39 25.095381109709216 24.823923142020227 25.054670123700262 25.0260256245703 40-41 25.173005320804425 24.89997942180939 24.94968713975734 24.977328117628844 42-43 24.636712341798265 25.121978019325336 25.449543750844324 24.791765888032078 44-45 24.725999526034872 24.663835182426066 25.415376530072876 25.194788761466185 46-47 25.044965209407362 24.62517818875553 25.014401805674364 25.315454796162744 48-49 24.677970503566033 25.162259789667647 25.09487450451372 25.064895202252597 50-51 24.443363177159558 25.028425165707972 25.719038088951095 24.809173568181368 52-53 24.910635277959862 24.927052034189405 24.87871579399219 25.28359689385854 54-55 24.974497644610906 24.963809921595203 24.98217707085138 25.079515362942512 56-57 24.306060007838433 24.801586654233994 25.609680662840358 25.28267267508722 58-59 24.85836027186658 24.656889145383566 25.36509169663609 25.119658886113765 60-61 24.889497994045424 24.423713144103505 25.454118268990733 25.23267059286034 62-63 24.621329462144008 24.58478287854568 25.463018175752307 25.330869483558004 64-65 24.8464211125195 24.69945306100868 25.313411057054182 25.140714769417638 66-67 24.76470631471896 25.24458423221823 24.734230818561638 25.25647863450117 68-69 24.5646024481146 25.431953156943816 24.965492927550017 25.03795146739157 70-71 24.814753352940787 25.405991996323014 24.667575631909084 25.111679018827115 72-73 24.746972360639397 25.488641036362296 24.982003438699532 24.782383164298775 74-75 25.078877356853457 25.253497127643776 24.877918016357306 24.78970749914546 76-77 25.265274063525194 25.18521489217214 24.729422991055593 24.82008805324707 78-79 24.85939161095385 25.20942389136357 24.774711246211474 25.156473251471105 80-81 25.010840003602127 25.311109611341777 24.59965374094115 25.078396644114946 82-83 24.957999695274385 25.306461478794983 24.57284349658792 25.16269532934271 84-85 24.941145791768836 25.156211754148078 24.515694410845317 25.386948043237766 86-87 24.84311049715549 25.53895549527948 24.688009877899063 24.929924129665967 88-89 24.9978330088953 25.435936057945614 24.686429685115193 24.8798012480439 90-91 25.21074195887179 25.15921899197816 24.70522129631454 24.92481775283551 92-93 25.052786299407977 25.400065349253243 24.99932421205024 24.547824139288544 94-95 24.913130216431647 25.326832341429412 24.799816363733846 24.96022107840509 96-97 24.711310417484086 25.577361851375418 24.64495778405572 25.066369947084777 98-99 24.972049719898486 25.17378321922485 24.923091351356195 24.931075709520474 100-101 25.109797214276586 25.32258237471885 24.72093617606873 24.846684234935836 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 2.0 3 3.0 4 3.0 5 6.5 6 10.5 7 11.5 8 17.0 9 28.5 10 39.5 11 66.5 12 109.5 13 152.0 14 186.0 15 241.5 16 363.0 17 555.0 18 765.0 19 1022.5 20 1476.5 21 2174.0 22 3142.0 23 4650.5 24 6673.0 25 9756.0 26 14035.5 27 19951.5 28 28646.0 29 39932.5 30 54267.5 31 72971.0 32 97446.0 33 128263.5 34 170455.5 35 230896.0 36 303986.5 37 400632.0 38 499448.0 39 583128.0 40 669943.5 41 753261.5 42 825618.0 43 887199.5 44 949585.0 45 960556.0 46 936047.0 47 946055.0 48 903665.5 49 845318.5 50 853291.0 51 807342.0 52 731361.0 53 689037.0 54 655141.0 55 646723.0 56 662742.0 57 668536.5 58 624780.5 59 535411.5 60 479388.5 61 438606.0 62 379643.5 63 343532.5 64 302370.5 65 275675.5 66 270053.5 67 267155.5 68 249466.0 69 209643.5 70 177232.5 71 152254.5 72 122445.5 73 100407.5 74 100068.0 75 89710.0 76 58719.5 77 35359.0 78 25483.0 79 26691.0 80 24417.0 81 15105.0 82 6668.0 83 1697.5 84 803.0 85 477.5 86 336.0 87 226.5 88 149.0 89 117.5 90 89.5 91 57.5 92 36.5 93 25.5 94 16.0 95 11.0 96 5.5 97 2.5 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1039529417462745 2 0.00886455069305461 3 0.0027925121890923048 4 0.0 5 2.6808117015286124E-5 6 0.0 7 0.0 8 4.468019502547687E-6 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001170621109667494 16-17 0.0020039067468926378 18-19 6.612668863770577E-4 20-21 3.3956948219362424E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.013622991463267897 32-33 0.045618479121011885 34-35 0.035918408780980855 36-37 0.033592804629904785 38-39 0.02348837852489319 40-41 0.04695665096202492 42-43 0.05034117573520479 44-45 0.03720519839771459 46-47 0.02896393642526538 48-49 0.011205792912389599 50-51 0.02815522489530425 52-53 0.034846084100369414 54-55 0.04386701547601319 56-57 0.030847206645589233 58-59 0.027319705248327834 60-61 0.048073655837661836 62-63 0.025595049720344425 64-65 0.03141241111266151 66-67 0.004492593609811699 68-69 0.019420246767823522 70-71 0.04328840695043327 72-73 0.061718987398442476 74-75 0.03618425594138244 76-77 0.009867621071376566 78-79 2.658471604015874E-4 80-81 2.7478319940668275E-4 82-83 0.0021937975757509143 84-85 0.006268631362074405 86-87 1.5638068258916906E-5 88-89 7.595633154331068E-5 90-91 0.0 92-93 0.0 94-95 3.6190957970636266E-4 96-97 4.669080380162333E-4 98-99 9.159439980222758E-5 100-101 9.382840955350142E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.2381281E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.20794359956367 #Duplication Level Percentage of deduplicated Percentage of total 1 66.73655013930365 26.833394440305735 2 17.979931118500097 14.458721126733831 3 6.272013607719949 7.565543081846987 4 3.1487781698131454 5.064235802375371 5 1.6484899108486863 3.3141194679926858 6 0.9354908954500912 2.2568499097297545 7 0.6725116544152221 1.8928217469543553 8 0.5373802654719442 1.728556432449159 9 0.32825822121904047 1.1878729240381445 >10 1.5277120961299806 10.752177121358836 >50 0.10622411787713391 2.9638280346343726 >100 0.08228775522012294 6.820509004119289 >500 0.012831448499277514 3.5382666408385255 >1k 0.010690963259407146 8.25418887635578 >5k 6.4884278657207E-4 1.6911138520161466 >10k+ 2.0079348572457235E-4 1.6778015382510134 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT 69141 0.3089233364256496 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTA 65383 0.29213251913507543 TruSeq Adapter, Index 7 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.3145619770378648 4.468019502547687E-6 0.0 2 0.31526792411926735 1.3404058507643062E-5 4.468019502547687E-6 3 0.3154153687628514 1.3404058507643062E-5 4.468019502547687E-6 4 0.3158040864595731 1.3404058507643062E-5 4.468019502547687E-6 5 0.3159247229861419 1.3404058507643062E-5 4.468019502547687E-6 6 0.31617046405878196 2.6808117015286124E-5 1.3404058507643062E-5 7 0.3163357807803762 2.6808117015286124E-5 1.3404058507643062E-5 8 0.31657258581401126 4.0212175522929186E-5 1.787207801019075E-5 9 0.3167691786721234 4.914821452802456E-5 1.787207801019075E-5 10-11 0.31716013037859625 5.5850243781846085E-5 2.0106087761464593E-5 12-13 0.3176404424751202 7.1488312040763E-5 2.457410726401228E-5 14-15 0.3181497966984106 8.265836079713221E-5 2.6808117015286124E-5 16-17 0.31864127884369087 8.936039005095375E-5 2.6808117015286124E-5 18-19 0.31902106050140744 9.382840955350143E-5 2.6808117015286124E-5 20-21 0.31976051772907904 1.0946647781241833E-4 3.57441560203815E-5 22-23 0.32013583136729307 1.2957256557388292E-4 4.0212175522929186E-5 24-25 0.3204686988202329 1.42976624081526E-4 5.5850243781846085E-5 26-27 0.32084401245844685 1.6308271184299058E-4 6.031826328439377E-5 28-29 0.32130421846720925 1.787207801019075E-4 7.1488312040763E-5 30-31 0.3216236818616414 1.898908288582767E-4 8.936039005095375E-5 32-33 0.3219855914413478 2.1893295562483667E-4 9.606241930477528E-5 34-35 0.3222559066212519 2.256349848786582E-4 1.072324680611445E-4 36-37 0.3225843060546892 2.4797508239139665E-4 1.1170048756369218E-4 38-39 0.32293057756613663 2.502090921426705E-4 1.1170048756369218E-4 40-41 0.32327238105808154 2.502090921426705E-4 1.1616850706623987E-4 42-43 0.3236789708328134 2.5467711164521816E-4 1.4074261433025216E-4 44-45 0.32407439055878884 2.725491896554089E-4 1.4744464358407367E-4 46-47 0.3244698102847643 2.7478319940668275E-4 1.4744464358407367E-4 48-49 0.3249054421862627 2.9488928716814734E-4 1.4744464358407367E-4 50-51 0.3255153268483605 3.1946339443215965E-4 1.4744464358407367E-4 52-53 0.3262034018517528 3.3063344318852883E-4 1.4744464358407367E-4 54-55 0.3269920072939525 3.351014626910765E-4 1.5191266308662137E-4 56-57 0.3278833771847107 3.41803491944898E-4 1.541466728378952E-4 58-59 0.3289065536507942 3.57441560203815E-4 1.5638068258916904E-4 60-61 0.32992079407787245 3.775476479652795E-4 1.586146923404429E-4 62-63 0.3318241703859578 4.311638819958518E-4 1.6978474109681212E-4 64-65 0.33336340310458545 4.579719990111379E-4 1.8095478985318133E-4 66-67 0.335798473733474 4.780780867726025E-4 1.8318879960445517E-4 68-69 0.33738685466662965 4.914821452802455E-4 1.8318879960445517E-4 70-71 0.3396096943691471 4.959501647827933E-4 1.8318879960445517E-4 72-73 0.3417766838278828 5.048862037878887E-4 1.8765681910700287E-4 74-75 0.34478366095309737 5.428643695595439E-4 1.8765681910700287E-4 76-77 0.34777276600030177 5.674384768235563E-4 1.898908288582767E-4 78-79 0.35185653582563037 5.786085255799255E-4 1.9659285811209824E-4 80-81 0.35705284250709335 5.9871461334139E-4 2.0106087761464592E-4 82-83 0.3634778545517569 6.121186718490331E-4 2.0329488736591975E-4 84-85 0.37186209314828766 6.255227303566762E-4 2.0552889711719362E-4 86-87 0.38222789839419824 6.2775674010795E-4 2.0552889711719362E-4 88-89 0.3951136666395458 6.478628278694146E-4 2.099969166197413E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTCACG 18665 0.0 38.180904 28-29 TATGCCG 19475 0.0 36.237705 48-49 CGTATGC 19785 0.0 35.788174 46-47 CACACGT 20230 0.0 35.481865 12-13 ACGAGTG 20135 0.0 35.134422 32-33 CTCGTAT 20515 0.0 34.29513 44-45 AGATCGG 21185 0.0 33.572952 1 CACGTCT 21520 0.0 33.28888 14-15 TCACGAG 22030 0.0 32.522278 30-31 CGCGTAT 7505 0.0 32.18099 1 AGCACAC 23985 0.0 30.193544 10-11 GTGGATA 24335 0.0 28.904688 36-37 CGTCTGA 25000 0.0 28.7872 16-17 GGATATC 25050 0.0 28.194153 38-39 GCGTATT 8690 0.0 27.980597 2 CCAGTCA 26810 0.0 26.836525 26-27 ATATCTC 26350 0.0 26.72197 40-41 GAGTGGA 26775 0.0 26.59864 34-35 ACATGCG 7600 0.0 26.429617 4 CCGTCTT 27040 0.0 26.187593 52-53 >>END_MODULE