FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n01 m48-2.3410000000cc92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n01 m48-2.3410000000cc92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22381281
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT691410.3089233364256496TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTA653830.29213251913507543TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG186650.038.18090428-29
TATGCCG194750.036.23770548-49
CGTATGC197850.035.78817446-47
CACACGT202300.035.48186512-13
ACGAGTG201350.035.13442232-33
CTCGTAT205150.034.2951344-45
AGATCGG211850.033.5729521
CACGTCT215200.033.2888814-15
TCACGAG220300.032.52227830-31
CGCGTAT75050.032.180991
AGCACAC239850.030.19354410-11
GTGGATA243350.028.90468836-37
CGTCTGA250000.028.787216-17
GGATATC250500.028.19415338-39
GCGTATT86900.027.9805972
CCAGTCA268100.026.83652526-27
ATATCTC263500.026.7219740-41
GAGTGGA267750.026.5986434-35
ACATGCG76000.026.4296174
CCGTCTT270400.026.18759352-53
Produced by FastQC (version 0.11.2)