FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n01 m12-1.3410000000cb6c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n01 m12-1.3410000000cb6c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20788236
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC634300.3051244944496493TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT37150.029.8183351
GAGCACA235400.029.573079
ATCGGAA229050.029.5248182
AGAGCAC234500.029.3618628
TCGGAAG233850.029.0991153
CGTATGC138300.027.77155344-45
TATGCCG138500.027.67963446-47
CACACGT150100.026.0854112-13
CTCGTAT147350.025.92428442-43
CGGAAGA285300.024.1004524
TCGTATG141300.023.65499142-43
CGTCTGA167400.023.5310516-17
GATCGGA290350.023.3989661
ACGTCTG147150.023.15540914-15
CACGTCT174000.022.63903614-15
GCGTATT47900.022.2085422
ACATGCG54600.022.0901934
TAAGTCG50050.021.5372719
ACACGTC159000.021.48925412-13
GTATGCC156850.021.44327244-45
Produced by FastQC (version 0.11.2)