FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n01 kx48-2.3410000000cd34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n01 kx48-2.3410000000cd34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16624912
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT42000.029.7688451
ACATGCG51000.026.2560064
CATGCGT53600.024.6280335
GCGTATT53550.023.14612
TAAGTCG54600.022.2639529
CGCGTGT24750.019.1860243
GTCCGTA50250.018.6157234
ATGCGTG79750.018.1601286
GCGTGCG73650.017.987938
TCCGTAC52500.017.9083525
CGTGCGA75500.017.5473779
TGCGTGC86550.016.8979267
CGTATTT83950.016.46013
TTAAGTC77000.016.2186538
CTGACAT117400.015.87352851
GTATTTA82550.015.760984
CCGTACC68500.015.1811026
TGTCCGT66850.014.1355233
GCGTGTA37600.013.8916744
AGTTACG32000.013.5033396
Produced by FastQC (version 0.11.2)