FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n01 kx12-1.3410000000cbe3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n01 kx12-1.3410000000cbe3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17814800
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT11950.017.1044731
CGAATTA58400.014.10771614-15
CGCGAAT24150.012.597141
CTCGGAT87850.012.4450081
CTCGGGT98750.012.2266021
TATGCCG74200.012.13263746-47
CGTATGC73950.012.04537544-45
CTCGGTT78650.011.6645421
CTCGATT98750.011.6489671
CACACGT78200.011.4768512-13
CTCGACT90800.011.0983691
CTCGTTT77600.011.0873061
TCGGGTC100650.010.8978978
CGTCTGA84650.010.6021816-17
CTCGCAT70750.010.5482971
CTCGTAT81900.010.44260142-43
CTGGAAT158950.010.377151
CCGCGTT50950.010.3559021
CTCGAAT81850.010.3373791
GAGTGTC85400.010.315557524-25
Produced by FastQC (version 0.11.2)