FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n01 kx0-1.3410000000c9d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n01 kx0-1.3410000000c9d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18402123
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC466300.2533946762555603TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG87450.030.53277828-29
ACGGCTA89900.029.70072432-33
CGTATGC97800.026.95922344-45
TATGCCG101300.025.95720146-47
AGAGCAC203300.025.2953858
GAGCACA207400.025.1623349
ACGTCTG104050.024.1896114-15
CTCGTAT108050.024.05317742-43
CGTCTGA112800.024.0386616-17
ATCGGAA214150.023.550192
CACACGT116400.023.15278412-13
TCGTATG107750.023.15005142-43
CACGGCT111050.022.71808230-31
ACACGTC111850.022.56615812-13
CACGTCT121050.022.3813314-15
TCGGAAG227500.022.250343
GTCACGG114750.022.04736928-29
CGGAAGA237550.021.4883794
GTATGCC118650.021.04060644-45
GGCTACA127700.021.03934134-35
Produced by FastQC (version 0.11.2)