FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l05n01 k12-1.3410000000cad7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l05n01 k12-1.3410000000cad7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21857922
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA367570.16816328651918513TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG125800.024.00003248-49
CGTATGC130050.023.28739446-47
CACACGT135050.022.96168112-13
ACCCGTC137550.022.3654932-33
GTCCCGA135900.022.27035736-37
CTCGTAT131700.021.87769344-45
ATCGGAA204700.019.9963933
CGTCTGA158450.019.70532816-17
CACGTCT161450.019.29553614-15
AGATCGG229550.017.8939251
AGCACAC182150.017.37559310-11
AGTCACC180600.017.3626528-29
AGAGCAC252750.016.5894439
TCGGAAG250100.016.5179774
TCGTATG127950.016.39235746-47
GATCGGA252450.016.2718052
CGGAAGA265700.015.5660315
CCGTCCC200900.015.466784534-35
CCAGTCA204500.015.390511526-27
TCACCCG206850.015.03335330-31
Produced by FastQC (version 0.11.2)