FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n02 tsrexp_lib#9.3420000000cb19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n02 tsrexp_lib#9.3420000000cb19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19586394
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCGC22600.022.59764942-43
TCGGGGG39850.020.98872438-39
GGCGCCC8150.020.98412744-45
GGGGGCG30650.017.2868840-41
GGGGGGC31550.016.64313540-41
CTCGGGG41300.014.32553836-37
CGGGGGG39750.014.22694138-39
GGGCGCC42650.014.14646542-43
AGGGTAG44950.014.0003328-29
GGGCCCG6900.013.76977744-45
CGGCAAC53050.012.709481
GTAGGGG31550.012.12223118-19
GGGTAGA55850.011.60811128-29
CGAAAAT101700.011.3918361
AGGGGCG23300.011.21402810-11
GGGGCCC11550.011.10523644-45
CGGGGGT17250.010.74427738-39
CGGGTAG23950.010.4145214-15
GATCGGG59500.010.374121
GGGCGGG27250.010.28607912-13
Produced by FastQC (version 0.11.2)