FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n02 tsrexp_lib#6.3420000000caae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n02 tsrexp_lib#6.3420000000caae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8903821
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCGC9200.021.68837242-43
GGCGCCC4950.019.1939644-45
TCGGGGG17150.017.59671638-39
GGGGGCG13650.015.84041340-41
GGGGGGC12950.015.77925440-41
GGGCGCC16200.014.5162942-43
GGGGCCC4600.013.42534144-45
CGGGGGG14800.013.32626338-39
AGGGTAG19150.013.02071828-29
CTCGGGG18000.012.54082336-37
GTAGGGG12250.012.41147418-19
GGGCCCG2551.8680657E-712.1091344-45
GGGTAGA23850.011.25134728-29
GCGCCCG7600.010.93866244-45
GGGTAGG10500.010.86010116-17
CGAAAAT49000.010.8522161
CGACTAT21800.010.67176151
CGGGTAG10500.010.6335514-15
CAAAAAT72550.010.60166551
CGGAAAT47050.010.4947041
Produced by FastQC (version 0.11.2)