FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n02 tsrexp_lib#40.3420000000ce66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n02 tsrexp_lib#40.3420000000ce66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7484917
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG3354.638501E-614.1743617
GGGCGCC5300.012.99789642-43
GGGGGCG5200.010.96590940-41
CGAAAAT51550.010.7766841
TAGGGTG5851.1013872E-610.5564069
CGAAAAC46250.010.1636911
GGGGCGC5154.5474735E-119.68641342-43
CAAAAAT82300.09.6348421
AAAAATC63800.09.3028762
CACGAAC13550.09.1109161
CTTGAAT49800.08.9624441
CGACGAT50800.08.6925511
CGGAAAT40150.08.633071
CACGAAT19150.08.4302041
CACATAG17700.08.3198799
TCGGGGG11300.08.20036338-39
TAGCCTA8209.029209E-68.1074238
CCGCGAT9407.8655466E-78.0820241
CGGGAAT19650.07.97405771
GATCCAC51500.07.92885455
Produced by FastQC (version 0.11.2)