FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l04n02 tsrexp_lib#37.3420000000ce09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l04n02 tsrexp_lib#37.3420000000ce09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416836
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC2850.019.16974344-45
GGGCGCC9750.016.81114242-43
GGGGGCG9350.015.75621340-41
TCGGGGG18050.012.90164938-39
GGGGGGC8900.012.54814840-41
GGGGCGC10600.012.1015442-43
CTCGGGG14650.012.00273236-37
CGGGCAT10750.011.92491
AGGGTAG19300.011.68866928-29
GTGTGCG13850.011.6562018
GGGTAGA19550.011.41773228-29
GCGGGCT11250.011.3949041
GCGGGAT23450.011.3382141
GTAGGGG11200.010.81820818-19
CGAAAAT59350.010.799721
GGGCGGG7450.010.522212-13
CAAAAAT93700.010.1342091
GGGGGTC9700.010.04347240-41
CACGAAT17350.09.8515011
CGCGAAT11850.09.6159531
Produced by FastQC (version 0.11.2)