##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	C5B16ACXX l04n02 tsrexp_lib#34.3420000000cdc4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7233938
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67904466419259	33.0	33.0	33.0	33.0	33.0
2	32.64461404009821	33.0	33.0	33.0	33.0	33.0
3	32.635030186877465	33.0	33.0	33.0	33.0	33.0
4	36.54942453197691	37.0	37.0	37.0	37.0	37.0
5	36.54677106715595	37.0	37.0	37.0	37.0	37.0
6	36.56018464631574	37.0	37.0	37.0	37.0	37.0
7	36.55947175660062	37.0	37.0	37.0	37.0	37.0
8	36.57460348706334	37.0	37.0	37.0	37.0	37.0
9	36.54881849968855	37.0	37.0	37.0	37.0	37.0
10-11	36.541821480913995	37.0	37.0	37.0	37.0	37.0
12-13	36.532776545776315	37.0	37.0	37.0	37.0	37.0
14-15	39.026761080893976	40.0	40.0	40.0	37.0	40.0
16-17	39.00811957470468	40.0	40.0	40.0	37.0	40.0
18-19	39.01048972219557	40.0	40.0	40.0	37.0	40.0
20-21	38.98760744147931	40.0	40.0	40.0	37.0	40.0
22-23	38.99139700948501	40.0	40.0	40.0	37.0	40.0
24-25	38.95456865955998	40.0	40.0	40.0	37.0	40.0
26-27	38.94147226586681	40.0	40.0	40.0	37.0	40.0
28-29	38.90103502407679	40.0	40.0	40.0	37.0	40.0
30-31	38.880050741380416	40.0	40.0	40.0	37.0	40.0
32-33	38.85777131349481	40.0	40.0	40.0	37.0	40.0
34-35	38.83119906474178	40.0	40.0	40.0	37.0	40.0
36-37	38.797561646229205	40.0	40.0	40.0	37.0	40.0
38-39	38.768494767303785	40.0	40.0	40.0	37.0	40.0
40-41	38.713459529235664	40.0	40.0	40.0	37.0	40.0
42-43	38.65826994923097	40.0	40.0	40.0	37.0	40.0
44-45	38.604459700926384	40.0	40.0	40.0	37.0	40.0
46-47	38.524763966735684	40.0	40.0	40.0	37.0	40.0
48-49	38.40728708208448	40.0	37.0	40.0	37.0	40.0
50-51	37.11706369338526	38.5	37.0	38.5	35.0	40.0
52-53	37.466946081097184	38.5	37.0	40.0	35.0	40.0
54-55	38.06920932969014	40.0	37.0	40.0	37.0	40.0
56-57	38.0058934704721	40.0	37.0	40.0	37.0	40.0
58-59	37.809153879947544	40.0	37.0	40.0	37.0	40.0
60-61	37.60853528188934	38.5	37.0	40.0	37.0	40.0
62-63	37.37555042633763	37.0	37.0	40.0	37.0	40.0
64-65	37.10483197395388	37.0	37.0	40.0	33.0	40.0
66-67	36.83816491100698	37.0	37.0	40.0	33.0	40.0
68-69	36.55936538300439	37.0	37.0	37.0	33.0	40.0
70-71	36.31477723475098	37.0	37.0	37.0	33.0	40.0
72-73	36.08058618970746	37.0	37.0	37.0	33.0	40.0
74-75	35.883466031917884	37.0	37.0	37.0	33.0	37.0
76-77	35.722090720158235	37.0	37.0	37.0	33.0	37.0
78-79	35.59742535808297	37.0	37.0	37.0	33.0	37.0
80-81	35.51583743183865	37.0	37.0	37.0	33.0	37.0
82-83	35.44370949820139	37.0	37.0	37.0	33.0	37.0
84-85	35.381792393023	37.0	37.0	37.0	33.0	37.0
86-87	35.32050758521845	37.0	37.0	37.0	33.0	37.0
88-89	35.263789031645004	37.0	37.0	37.0	33.0	37.0
90-91	35.203740341705995	37.0	37.0	37.0	33.0	37.0
92-93	35.15989962866699	37.0	37.0	37.0	33.0	37.0
94-95	35.04831020116567	37.0	37.0	37.0	33.0	37.0
96-97	35.03627249777369	37.0	37.0	37.0	33.0	37.0
98-99	34.990939236692384	37.0	33.0	37.0	33.0	37.0
100-101	33.185280894030335	35.0	33.0	37.0	27.5	37.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2306	1	0.09873992323930025
2306	2	0.13841543774611154
2306	3	0.11929614078695039
2306	4	0.13475905553507062
2306	5	0.10332176976857
2306	6	0.14346302572516834
2306	7	0.14408308682098436
2306	8	0.15192310997591818
2306	9	0.1503144459377026
2306	10-11	0.10974789820526354
2306	12-13	0.15452416628122023
2306	14-15	0.2495860313472491
2306	16-17	0.2887952875266109
2306	18-19	0.28055024982572263
2306	20-21	0.29271375454826654
2306	22-23	0.2601432865560227
2306	24-25	0.34625616185748953
2306	26-27	0.3170135079734777
2306	28-29	0.3544463564730691
2306	30-31	0.3085274091613073
2306	32-33	0.3377972864436103
2306	34-35	0.3312684860604733
2306	36-37	0.29458663071140734
2306	38-39	0.3130822643354563
2306	40-41	0.34886797539500236
2306	42-43	0.3316704331612499
2306	44-45	0.33540231019984645
2306	46-47	0.36030063099651244
2306	48-49	0.40898496399853457
2306	50-51	0.12481826659789164
2306	52-53	0.20808757931524724
2306	54-55	0.4506924905312317
2306	56-57	0.47860344988163916
2306	58-59	0.4620446325846643
2306	60-61	0.44766182984130864
2306	62-63	0.4690060729264687
2306	64-65	0.44679524400626036
2306	66-67	0.39014426449912065
2306	68-69	0.399537888155983
2306	70-71	0.441324112384315
2306	72-73	0.43807317635461374
2306	74-75	0.41892424233810743
2306	76-77	0.4123907941127456
2306	78-79	0.4197947782726672
2306	80-81	0.4510646115124928
2306	82-83	0.41737004302647307
2306	84-85	0.4194666754877545
2306	86-87	0.48504153204461886
2306	88-89	0.4405993740551821
2306	90-91	0.48425836174371995
2306	92-93	0.484251603383143
2306	94-95	0.6491430470720019
2306	96-97	0.519683004706124
2306	98-99	0.4846402619435324
2306	100-101	0.41809478950045076
2307	1	0.13193546901236886
2307	2	0.10522882550577606
2307	3	0.10560736979903851
2307	4	0.0942638389545607
2307	5	0.13839027128540238
2307	6	0.15117851187421394
2307	7	0.15031544410901887
2307	8	0.12756932518732356
2307	9	0.18439376173954258
2307	10-11	0.15886738485523466
2307	12-13	0.17930586728757447
2307	14-15	0.2585864843585739
2307	16-17	0.22967089393075213
2307	18-19	0.24049342606043922
2307	20-21	0.29660406441028897
2307	22-23	0.29287924267710963
2307	24-25	0.29378481372729226
2307	26-27	0.3045239419336312
2307	28-29	0.3179158417180403
2307	30-31	0.2902136670652524
2307	32-33	0.30180903712527396
2307	34-35	0.326851178981336
2307	36-37	0.3302304517360355
2307	38-39	0.32060413646606634
2307	40-41	0.38061312152242266
2307	42-43	0.34096837281246906
2307	44-45	0.3327004850466224
2307	46-47	0.30048681231571805
2307	48-49	0.37998850346420454
2307	50-51	0.25123944953081434
2307	52-53	0.30955163914457984
2307	54-55	0.372048327920794
2307	56-57	0.3659105891224428
2307	58-59	0.33031425115042623
2307	60-61	0.4071227577364951
2307	62-63	0.3812526306332771
2307	64-65	0.3607170015277674
2307	66-67	0.3677288938850154
2307	68-69	0.36588965591531775
2307	70-71	0.36600224938214154
2307	72-73	0.4258054055682763
2307	74-75	0.41054986754327416
2307	76-77	0.34836570810312395
2307	78-79	0.38604990145065443
2307	80-81	0.37377923923025946
2307	82-83	0.40391167596905575
2307	84-85	0.35838096538803654
2307	86-87	0.41775440716568824
2307	88-89	0.404469812491854
2307	90-91	0.40786585545085785
2307	92-93	0.4441307830379486
2307	94-95	0.583762744147279
2307	96-97	0.5070728359744265
2307	98-99	0.42334063434190483
2307	100-101	0.597544691791974
2201	1	0.1341898759162703
2201	2	0.10984740535893422
2201	3	0.1061066929002763
2201	4	0.1174799440085863
2201	5	0.13830792231549083
2201	6	0.08558714192896844
2201	7	0.09524675749298694
2201	8	0.08111175061910814
2201	9	0.10503398612753756
2201	10-11	0.13188421762001923
2201	12-13	0.09444393425473407
2201	14-15	0.1917855375555746
2201	16-17	0.19376806258745205
2201	18-19	0.2133406003984959
2201	20-21	0.19685993756569786
2201	22-23	0.25191788760697165
2201	24-25	0.2822200616082071
2201	26-27	0.23677048390000976
2201	28-29	0.2840262594198677
2201	30-31	0.2817165240369164
2201	32-33	0.24908419279292815
2201	34-35	0.2667496996370673
2201	36-37	0.2882182309534116
2201	38-39	0.2723432172003655
2201	40-41	0.3298600585194933
2201	42-43	0.34928671838892456
2201	44-45	0.3482193561316791
2201	46-47	0.2979078478395891
2201	48-49	0.35491321769006134
2201	50-51	0.283717809956471
2201	52-53	0.33241926388408416
2201	54-55	0.3460224369159377
2201	56-57	0.36942862508630725
2201	58-59	0.3207724491272259
2201	60-61	0.35214929780762105
2201	62-63	0.3339065424463783
2201	64-65	0.36525596923137726
2201	66-67	0.39056045124185346
2201	68-69	0.38065834498960527
2201	70-71	0.4290571000028294
2201	72-73	0.40059046864783454
2201	74-75	0.4002188105114044
2201	76-77	0.3506953042593821
2201	78-79	0.4484933170787073
2201	80-81	0.4136888733315516
2201	82-83	0.42361741793058627
2201	84-85	0.4032011149933936
2201	86-87	0.4537364108124038
2201	88-89	0.4230991287935595
2201	90-91	0.435430254911104
2201	92-93	0.39891083773623137
2201	94-95	0.5573808465719026
2201	96-97	0.49880629449774005
2201	98-99	0.47912553707311645
2201	100-101	0.5993151702091879
1101	1	0.13206441804197055
1101	2	0.15021798970168732
1101	3	0.13891473429433177
1101	4	0.20237830437774562
1101	5	0.1380465755272553
1101	6	0.19352410619909222
1101	7	0.14106200849418116
1101	8	0.1698028637694975
1101	9	0.16621144360323825
1101	10-11	0.15180243324279985
1101	12-13	0.18322556890439046
1101	14-15	0.27126659468459025
1101	16-17	0.25520305427320267
1101	18-19	0.29815944127973637
1101	20-21	0.24636007806465443
1101	22-23	0.20975502115121714
1101	24-25	0.24229563605807414
1101	26-27	0.28518096776672763
1101	28-29	0.30960136069610655
1101	30-31	0.2991268039415971
1101	32-33	0.3659559231531091
1101	34-35	0.31033799103952475
1101	36-37	0.32775166027077063
1101	38-39	0.3190727462371541
1101	40-41	0.29544173660203654
1101	42-43	0.36250150653877
1101	44-45	0.3330476696357536
1101	46-47	0.37961074481950874
1101	48-49	0.42987109741554974
1101	50-51	0.23905753049840683
1101	52-53	0.2927100420330433
1101	54-55	0.42223119086560956
1101	56-57	0.36202318214511564
1101	58-59	0.4032232214444562
1101	60-61	0.4195889476360435
1101	62-63	0.3735394909475076
1101	64-65	0.40421276243744586
1101	66-67	0.3544278634541982
1101	68-69	0.42697249907936907
1101	70-71	0.4485083510491634
1101	72-73	0.42429745018436194
1101	74-75	0.4180314590249452
1101	76-77	0.3855896755824517
1101	78-79	0.4172390864785527
1101	80-81	0.38002075787064626
1101	82-83	0.40703941295122803
1101	84-85	0.3834168624750518
1101	86-87	0.40987178374289357
1101	88-89	0.41665260373200397
1101	90-91	0.39119966390789074
1101	92-93	0.39110550980853986
1101	94-95	0.5031991868507362
1101	96-97	0.4461044815536326
1101	98-99	0.4181312358535507
1101	100-101	0.24520950933230523
2304	1	0.09547994536863769
2304	2	0.09263984636962164
2304	3	0.0700225610598153
2304	4	0.12392121096090847
2304	5	0.07016884938776968
2304	6	0.14391066516189
2304	7	0.09671661707348989
2304	8	0.14943002929041427
2304	9	0.10379038931844065
2304	10-11	0.12542999653427245
2304	12-13	0.11796239112194229
2304	14-15	0.2086102874166329
2304	16-17	0.22402629020044174
2304	18-19	0.2170945054696034
2304	20-21	0.17379212313246484
2304	22-23	0.2305874376788708
2304	24-25	0.21710037783142866
2304	26-27	0.24178642369217584
2304	28-29	0.2108568026342752
2304	30-31	0.2463495669603759
2304	32-33	0.25217091860549345
2304	34-35	0.2277257658615781
2304	36-37	0.2334303170191916
2304	38-39	0.2692936776142645
2304	40-41	0.2991110844620337
2304	42-43	0.28694807598541416
2304	44-45	0.29131253086779196
2304	46-47	0.2898973511011391
2304	48-49	0.35866252880332183
2304	50-51	0.20693009401768592
2304	52-53	0.30582364160311215
2304	54-55	0.3152487548148386
2304	56-57	0.33449846648964154
2304	58-59	0.3501664332442189
2304	60-61	0.4040626978115611
2304	62-63	0.3648910913519998
2304	64-65	0.3369342190178628
2304	66-67	0.3247469763605437
2304	68-69	0.30153857463120914
2304	70-71	0.29822041445981995
2304	72-73	0.33768085108530244
2304	74-75	0.2967531328397115
2304	76-77	0.31209480096026
2304	78-79	0.30777451083893936
2304	80-81	0.36110274821001553
2304	82-83	0.37810487046589714
2304	84-85	0.34134889409205726
2304	86-87	0.3354247788945415
2304	88-89	0.34737125134942914
2304	90-91	0.36593246721221107
2304	92-93	0.364418553681638
2304	94-95	0.44191134325075865
2304	96-97	0.37709329204083986
2304	98-99	0.3374748195796684
2304	100-101	0.4079664371565457
2202	1	0.0742065069955089
2202	2	0.0905888105309458
2202	3	0.1233899775871592
2202	4	0.1320068937385983
2202	5	0.12727083895526192
2202	6	0.1213826280664847
2202	7	0.12489198776528809
2202	8	0.1099852230810825
2202	9	0.1365038977586508
2202	10-11	0.11507125343092639
2202	12-13	0.1667784838559001
2202	14-15	0.2358256427584564
2202	16-17	0.2435069966645358
2202	18-19	0.25737459417106834
2202	20-21	0.24752649089796108
2202	22-23	0.24663699268905503
2202	24-25	0.22893496010892989
2202	26-27	0.2859593892493777
2202	28-29	0.2808823279663102
2202	30-31	0.28799879478558665
2202	32-33	0.29840047952302484
2202	34-35	0.29485784527093273
2202	36-37	0.2961354491134287
2202	38-39	0.29192164354780203
2202	40-41	0.34998883314656126
2202	42-43	0.2935332581210446
2202	44-45	0.3312103668168547
2202	46-47	0.352624222350606
2202	48-49	0.41024740587801745
2202	50-51	0.232172888307403
2202	52-53	0.2697188249475815
2202	54-55	0.3640093948839933
2202	56-57	0.3754902452400657
2202	58-59	0.3898723944199318
2202	60-61	0.39066425060021714
2202	62-63	0.37193407871392736
2202	64-65	0.3536548368033152
2202	66-67	0.34577964468005007
2202	68-69	0.3920339736281022
2202	70-71	0.35963666411484496
2202	72-73	0.3742118977351083
2202	74-75	0.34814684790025296
2202	76-77	0.40361671573935354
2202	78-79	0.3119291128684196
2202	80-81	0.3366211807328199
2202	82-83	0.36502823402782525
2202	84-85	0.380135538467421
2202	86-87	0.42629772643318375
2202	88-89	0.45956239853819625
2202	90-91	0.4319790143786264
2202	92-93	0.41313393354236894
2202	94-95	0.5442359788312388
2202	96-97	0.47902804834562573
2202	98-99	0.3937347554427504
2202	100-101	0.3924825991136771
1102	1	0.05697310060346439
1102	2	0.06372885447777321
1102	3	0.08483607130838777
1102	4	0.10412310674565362
1102	5	0.09554623866711864
1102	6	0.08994929543992924
1102	7	0.09196465290902722
1102	8	0.008039239840691437
1102	9	0.10698968144163246
1102	10-11	0.08356255808686086
1102	12-13	0.10587344632031659
1102	14-15	0.1903692997076405
1102	16-17	0.1637548280596164
1102	18-19	0.1851973028375653
1102	20-21	0.17850777276581908
1102	22-23	0.2143003885463628
1102	24-25	0.22733016317605603
1102	26-27	0.2141650493277396
1102	28-29	0.20567253294732524
1102	30-31	0.2085936922448539
1102	32-33	0.2757987785645142
1102	34-35	0.25488491586595785
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1213	88-89	-0.8507263281749005
1213	90-91	-0.9214155500808161
1213	92-93	-0.8233329767372197
1213	94-95	-0.785183606495174
1213	96-97	-0.8019683902996491
1213	98-99	-0.9127128623465097
1213	100-101	-0.9303854628364903
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12547.0
3	791.0
4	583.0
5	427.0
6	524.0
7	1318.0
8	2007.0
9	2753.0
10	4764.0
11	7040.0
12	6478.0
13	6130.0
14	5622.0
15	5495.0
16	5832.0
17	6097.0
18	6940.0
19	7659.0
20	8374.0
21	9259.0
22	10594.0
23	11902.0
24	13598.0
25	15394.0
26	17030.0
27	19843.0
28	24099.0
29	29865.0
30	37639.0
31	49051.0
32	66414.0
33	93413.0
34	136032.0
35	215641.0
36	478406.0
37	2678236.0
38	3234754.0
39	1387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.899186584249403	14.844747422844614	20.65843325018663	37.597632742719355
2	25.048944222975145	24.90028341668901	31.260885727011452	18.789886633324397
3	23.320789303085476	27.516172603234118	29.847151917009697	19.31588617667071
4	25.47226516374269	31.59359006284127	21.284028865934836	21.650115907481204
5	26.932360961274536	34.19908791648606	21.52759447321153	17.34095664902788
6	18.962903135565007	37.71653501869329	22.553890359645756	20.766671486095948
7	17.949387141737446	16.078203047100086	43.49426317234531	22.478146638817154
8	22.012395087779264	21.521690988126625	28.031861211801328	28.43405271229278
9	24.27345353367308	23.15945509413243	26.81440104802589	25.752690324168604
10-11	24.133814579950627	31.57513989232242	21.850959603290647	22.440085924436307
12-13	22.736288770654898	25.586776266565543	28.132040380290302	23.544894582489263
14-15	22.938567094222094	26.884481042712977	27.902150868517506	22.274800994547423
16-17	23.795526648218214	27.314197583350847	26.871862932874635	22.018412835556305
18-19	23.657607250803014	27.52717433290452	26.94822338711281	21.866995029179655
20-21	23.813073675858604	27.65910170216553	26.75586501219397	21.7719596097819
22-23	23.901903299014208	27.555729344340236	26.818450520538295	21.723916836107268
24-25	23.383295603082846	28.0178253435596	26.78377109153797	21.81510796181958
26-27	23.699841245195284	28.073701362070913	26.60538050816919	21.621076884564616
28-29	23.772691348879178	27.529016388411005	26.88531784360745	21.812974419102368
30-31	23.518718413648664	27.591113532782842	26.93422892097004	21.955939132598463
32-33	23.636660349999424	27.756287378944204	26.786691194490132	21.820361076566236
34-35	23.771162937383767	27.5907143370825	26.808609019811126	21.829513705722604
36-37	23.38666832948806	27.613303956822072	27.077050986260936	21.92297672742893
38-39	23.724410927335175	27.60845540217413	26.773585598544393	21.893548071946302
40-41	24.10091661460677	27.413929258991526	26.724159922449473	21.760994203952226
42-43	23.794294617576696	27.422600513533062	26.99154485334274	21.791560015547496
44-45	24.097228289892204	27.487370719942582	26.712759170731264	21.70264181943395
46-47	24.033844160218376	27.37844784247531	26.76449127249368	21.82321672481263
48-49	23.740224787187735	27.421657096037162	26.905265147937207	21.932852968837892
50-51	24.203679696627745	27.570753697377086	26.64638171386259	21.579184892132584
52-53	24.227798385337117	27.422579650814434	26.74308434552816	21.606537618320292
54-55	24.008597381549087	27.49728829325475	26.712940365727462	21.781173959468696
56-57	24.27789420854308	27.511189563581052	26.680588040839293	21.530328187036574
58-59	24.429639932730023	27.621514729835784	26.515006537289494	21.433838800144702
60-61	24.25683180253243	27.766514452453457	26.515086967972394	21.461566777041714
62-63	24.57748615980268	27.790810043867616	26.21829098422175	21.413412812107957
64-65	24.70767195968793	27.735600917271668	26.270902173622385	21.285824949418018
66-67	24.540382780381705	27.85836956893611	26.329915761832744	21.271331888849442
68-69	24.97174991763062	27.830107623550088	26.027497831938206	21.170644626881085
70-71	25.17186643321082	27.77771814341246	25.95443705186073	21.095978371515987
72-73	25.10351039965298	27.880379857371363	26.022511010293353	20.993598732682305
74-75	25.48127523986423	27.989598198440007	25.716752040026414	20.812374521669348
76-77	25.738922452838164	27.795531208697227	25.699555948604534	20.765990389860075
78-79	25.737646268635306	27.91674049290683	25.64197300485351	20.703640233604357
80-81	26.246217804316863	27.94788893247003	25.24053025763474	20.565363005578373
82-83	26.480972509413597	27.837030622172875	25.271852955060737	20.410143913352794
84-85	26.479017982923825	27.906555063994787	25.297035038041706	20.317391915039682
86-87	26.849741277243133	27.960021809057135	24.9695414486396	20.220695465060132
88-89	27.226643042152325	27.878007819315147	24.95235394976853	19.942995188763998
90-91	27.27185280621878	27.981208319550877	24.918027742601186	19.828911131629155
92-93	27.689816514403415	28.04386849925258	24.613779893075677	19.652535093268327
94-95	28.043693415893024	28.002507328629505	24.542197068880732	19.41160218659674
96-97	28.003942611133965	28.15701773766256	24.525898378484435	19.313141272719047
98-99	28.467359455228152	28.24145887697754	24.294957000119023	18.99622466767529
100-101	28.639458335335593	28.240102235161647	24.1759969777035	18.944442451799258
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10732.0
1	5962.0
2	845.5
3	454.0
4	381.0
5	339.0
6	300.0
7	266.0
8	267.5
9	270.0
10	286.0
11	310.5
12	311.5
13	322.0
14	361.0
15	393.0
16	408.5
17	447.0
18	531.5
19	613.0
20	703.0
21	896.0
22	1173.0
23	1532.5
24	2237.0
25	3330.5
26	4883.0
27	7054.0
28	10130.0
29	14374.0
30	19211.5
31	25018.0
32	32035.0
33	40074.5
34	50483.0
35	64618.0
36	84791.5
37	110529.0
38	141709.5
39	179608.0
40	225499.0
41	276973.0
42	331738.0
43	387453.5
44	437320.5
45	476214.5
46	502945.0
47	514110.5
48	505492.5
49	480204.5
50	439939.5
51	387998.0
52	328979.5
53	272185.0
54	216724.0
55	165696.5
56	126174.0
57	95003.0
58	70833.5
59	51520.5
60	36384.0
61	24911.0
62	17844.5
63	12987.0
64	9108.5
65	6773.5
66	5073.0
67	3748.0
68	3048.5
69	2402.5
70	1832.0
71	1283.5
72	748.0
73	450.0
74	413.5
75	360.0
76	281.0
77	142.5
78	60.0
79	43.5
80	32.5
81	29.5
82	25.5
83	18.5
84	18.5
85	19.5
86	17.0
87	12.0
88	6.5
89	5.5
90	3.5
91	3.5
92	6.0
93	5.5
94	4.0
95	2.0
96	0.5
97	0.5
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19032510369870465
2	0.18626092731234356
3	0.1830123509490958
4	0.18695211377260906
5	0.1812014424232002
6	0.18200321871710817
7	0.20929126016838961
8	0.18657887308406568
9	0.1972507920305648
10-11	0.14673197364976034
12-13	0.1932557342902303
14-15	0.24872344772653565
16-17	0.2584968242746897
18-19	0.2422401187292454
20-21	0.272548645011887
22-23	0.25273232919607547
24-25	0.23996611527497194
26-27	0.2414106949769268
28-29	0.23892242371997105
30-31	0.19282028682026303
32-33	0.2138392670769365
34-35	0.23686959993298257
36-37	0.23898463050139496
38-39	0.2449979527057047
40-41	0.29157009639839326
42-43	0.25751533950111266
44-45	0.24702312903428256
46-47	0.22569311487048963
48-49	0.26361160408065426
50-51	0.23403573544589404
52-53	0.2172122570030321
54-55	0.22337764022860024
56-57	0.18174056786220727
58-59	0.20690666688047368
60-61	0.21870521975720555
62-63	0.21762005701458872
64-65	0.22735887423972947
66-67	0.197527266614671
68-69	0.20642283635828781
70-71	0.19373265294781347
72-73	0.18868007992327276
74-75	0.23919889830407723
76-77	0.27209246194811176
78-79	0.2520549664650153
80-81	0.224483538565025
82-83	0.2017435040222905
84-85	0.20398294815355067
86-87	0.23158893537655423
88-89	0.2447491255800091
90-91	0.2647175024170791
92-93	0.2319276167420843
94-95	0.23078715908264627
96-97	0.23353116932990026
98-99	0.23258424387933652
100-101	0.17886523218750286
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7233938.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.73706780798617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.61359713657063	37.56814598836545
2	13.729145588667619	14.206114725332217
3	5.131285307212357	7.964329676441066
4	2.7693138861767754	5.73104721242902
5	1.3896355127135476	3.5947833374823213
6	0.9341562157159493	2.8998302085448695
7	0.6464223638543508	2.341079836993188
8	0.5087389192218554	2.1056527968274183
9	0.34804926819614224	1.6206343730265396
>10	1.8080731339977192	16.09099354022864
>50	0.09308113195158647	3.3435426259105774
>100	0.027860824772413695	2.2386496683490638
>500	5.454406187271163E-4	0.17247985569390833
>1k	6.847587300643049E-5	0.048744965562012396
>5k	2.679445735259596E-5	0.07397118881375549
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.7647458410619497E-4	1.382372920530975E-5	0.0
2	3.179457717221242E-4	1.382372920530975E-5	0.0
3	3.4559323013274375E-4	1.382372920530975E-5	0.0
4	4.8383052218584126E-4	1.382372920530975E-5	0.0
5	4.8383052218584126E-4	1.382372920530975E-5	0.0
6	5.391254390070803E-4	1.382372920530975E-5	0.0
7	5.391254390070803E-4	1.382372920530975E-5	0.0
8	5.529491682123899E-4	1.382372920530975E-5	1.382372920530975E-5
9	6.08244085033629E-4	1.382372920530975E-5	1.382372920530975E-5
10-11	7.948644293053106E-4	1.382372920530975E-5	1.382372920530975E-5
12-13	9.261898567557533E-4	1.382372920530975E-5	1.382372920530975E-5
14-15	0.001098986471822125	1.382372920530975E-5	1.382372920530975E-5
16-17	0.0012925186806964615	1.382372920530975E-5	1.382372920530975E-5
18-19	0.0016726712338424798	1.382372920530975E-5	1.382372920530975E-5
20-21	0.0027854814348699146	1.382372920530975E-5	1.382372920530975E-5
22-23	0.008370268033815053	2.76474584106195E-5	1.382372920530975E-5
24-25	0.015821258075477008	2.76474584106195E-5	1.382372920530975E-5
26-27	0.029140421164792953	2.76474584106195E-5	1.382372920530975E-5
28-29	0.059469683041242546	4.147118761592925E-5	1.382372920530975E-5
30-31	0.10864759968913199	5.5294916821239E-5	1.382372920530975E-5
32-33	0.17535400496935416	5.5294916821239E-5	1.382372920530975E-5
34-35	0.24611076290673212	5.5294916821239E-5	1.382372920530975E-5
36-37	0.33280628061783224	5.5294916821239E-5	1.382372920530975E-5
38-39	0.4427118396646474	7.603051062920362E-5	1.382372920530975E-5
40-41	0.5815366402089706	8.29423752318585E-5	1.382372920530975E-5
42-43	0.7511537975581212	8.29423752318585E-5	1.382372920530975E-5
44-45	0.9442229115040798	8.29423752318585E-5	1.382372920530975E-5
46-47	1.162354446499265	8.29423752318585E-5	1.382372920530975E-5
48-49	1.4102830297964952	8.29423752318585E-5	1.382372920530975E-5
50-51	1.6947200819249488	8.29423752318585E-5	1.382372920530975E-5
52-53	2.021257301348173	8.985423983451338E-5	1.382372920530975E-5
54-55	2.383473565850302	1.10589833642478E-4	1.382372920530975E-5
56-57	2.784071414490973	1.1750169824513288E-4	1.382372920530975E-5
58-59	3.2358516205143033	1.3823729205309748E-4	1.382372920530975E-5
60-61	3.747585340101063	1.3823729205309748E-4	1.382372920530975E-5
62-63	4.329377719300331	1.3823729205309748E-4	1.382372920530975E-5
64-65	4.9846916022780405	1.3823729205309748E-4	1.382372920530975E-5
66-67	5.7187800061322065	1.3823729205309748E-4	1.382372920530975E-5
68-69	6.549731280527977	1.3823729205309748E-4	1.382372920530975E-5
70-71	7.471566662584059	1.3823729205309748E-4	1.382372920530975E-5
72-73	8.493672464430855	1.3823729205309748E-4	1.382372920530975E-5
74-75	9.605667065435174	1.3823729205309748E-4	1.382372920530975E-5
76-77	10.793422614349197	1.5206102125840725E-4	1.382372920530975E-5
78-79	12.056199541660435	1.5897288586106214E-4	1.382372920530975E-5
80-81	13.403888725615287	1.65884750463717E-4	1.382372920530975E-5
82-83	14.821346270869338	1.65884750463717E-4	1.382372920530975E-5
84-85	16.30337445524139	1.7279661506637187E-4	1.382372920530975E-5
86-87	17.850201370263335	1.935322088743365E-4	1.382372920530975E-5
88-89	19.437794462711736	2.1426780268230113E-4	1.382372920530975E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCGCC	870	0.0	27.577007	42-43
GGCGCCC	295	0.0	27.376116	44-45
GGGGCGC	890	0.0	23.487547	42-43
GGGGGCG	1005	0.0	20.807272	40-41
TCGGGGG	1480	0.0	19.910797	38-39
GGGCCCG	240	0.0	17.814604	44-45
CGGGTAG	680	0.0	16.76907	14-15
CTCGGGG	1250	0.0	16.729511	36-37
GGGGGGC	1260	0.0	15.841901	40-41
GGGTAGA	1825	0.0	15.484322	28-29
GTAGGGG	1140	0.0	15.419984	18-19
CGGGGGG	1610	0.0	13.874925	38-39
AGGGTAG	1925	0.0	13.569694	28-29
GAGCGGC	1765	0.0	13.457028	9
GGGGCCC	460	0.0	12.909134	44-45
GGGCGGG	800	0.0	12.17441	12-13
GCGCCCG	540	0.0	11.876403	44-45
TAGGGGA	1555	0.0	11.762981	18-19
CGCGAAT	970	0.0	11.25782	1
CGAAAAT	5025	0.0	11.149212	1
>>END_MODULE